{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,16]],"date-time":"2026-10-16T21:24:37Z","timestamp":1792185877252,"version":"4.4.0"},"reference-count":58,"publisher":"Public Library of Science (PLoS)","issue":"7","license":[{"start":{"date-parts":[[2018,7,5]],"date-time":"2018-07-05T00:00:00Z","timestamp":1530748800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/publicdomain\/zero\/1.0\/"}],"funder":[{"DOI":"10.13039\/100000060","name":"National Institute of Allergy and Infectious Diseases","doi-asserted-by":"publisher","award":["R01-AI117235"],"award-info":[{"award-number":["R01-AI117235"]}],"id":[{"id":"10.13039\/100000060","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["www.plospathogens.org"],"crossmark-restriction":false},"short-container-title":["PLoS Pathog"],"DOI":"10.1371\/journal.ppat.1007081","type":"journal-article","created":{"date-parts":[[2018,7,5]],"date-time":"2018-07-05T17:46:39Z","timestamp":1530812799000},"page":"e1007081","update-policy":"https:\/\/doi.org\/10.1371\/journal.ppat.corrections_policy","source":"Crossref","is-referenced-by-count":33,"title":["SliC is a surface-displayed lipoprotein that is required for the anti-lysozyme strategy during Neisseria gonorrhoeae infection"],"prefix":"10.1371","volume":"14","author":[{"given":"Ryszard A.","family":"Zielke","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Adriana","family":"Le Van","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Benjamin I.","family":"Baarda","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7593-907X","authenticated-orcid":true,"given":"Marco F.","family":"Herrera","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christopher J.","family":"Acosta","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5118-3172","authenticated-orcid":true,"given":"Ann E.","family":"Jerse","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7977-2596","authenticated-orcid":true,"given":"Aleksandra E.","family":"Sikora","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"340","published-online":{"date-parts":[[2018,7,5]]},"reference":[{"key":"ref1","first-page":"75","article-title":"Plant lysozymes","volume":"75","author":"JJ Beintema","year":"1996","journal-title":"EXS"},{"issue":"2","key":"ref2","doi-asserted-by":"crossref","first-page":"327","DOI":"10.1007\/s12038-012-9201-y","article-title":"Invertebrate lysozymes: diversity and distribution, molecular mechanism and in vivo function","volume":"37","author":"JM Van Herreweghe","year":"2012","journal-title":"J Biosci"},{"issue":"1","key":"ref3","doi-asserted-by":"crossref","first-page":"127","DOI":"10.1007\/s12038-010-0015-5","article-title":"Lysozymes in the animal kingdom","volume":"35","author":"L Callewaert","year":"2010","journal-title":"J Biosci"},{"issue":"2","key":"ref4","doi-asserted-by":"crossref","first-page":"149","DOI":"10.1111\/j.1574-6976.2007.00094.x","article-title":"Peptidoglycan structure and architecture","volume":"32","author":"W Vollmer","year":"2008","journal-title":"FEMS Microbiol Rev"},{"key":"ref5","first-page":"9","article-title":"Animal lysozymes c and g: an overview","volume":"75","author":"EM Prager","year":"1996","journal-title":"EXS"},{"key":"ref6","doi-asserted-by":"crossref","first-page":"166","DOI":"10.1186\/1471-2148-11-166","article-title":"Evolution of the mammalian lysozyme gene family","volume":"11","author":"DM Irwin","year":"2011","journal-title":"BMC Evol Biol"},{"issue":"2","key":"ref7","doi-asserted-by":"crossref","first-page":"562","DOI":"10.1128\/IAI.00651-10","article-title":"Modifications to the peptidoglycan backbone help bacteria to establish infection","volume":"79","author":"KM Davis","year":"2011","journal-title":"Infection and immunity"},{"issue":"10","key":"ref8","doi-asserted-by":"crossref","first-page":"501","DOI":"10.1016\/j.tim.2012.06.005","article-title":"Guards of the great wall: bacterial lysozyme inhibitors","volume":"20","author":"L Callewaert","year":"2012","journal-title":"Trends Microbiol"},{"issue":"6","key":"ref9","doi-asserted-by":"crossref","first-page":"e1006448","DOI":"10.1371\/journal.ppat.1006448","article-title":"Structure of the Neisseria Adhesin Complex Protein (ACP) and its role as a novel lysozyme inhibitor","volume":"13","author":"MV Humbert","year":"2017","journal-title":"PLoS Pathog"},{"issue":"4","key":"ref10","doi-asserted-by":"crossref","first-page":"295","DOI":"10.1007\/s10858-006-9032-y","article-title":"The solution structure of the protein ydhA from Escherichia coli","volume":"35","author":"M Revington","year":"2006","journal-title":"J Biomol NMR"},{"issue":"2","key":"ref11","doi-asserted-by":"crossref","first-page":"244","DOI":"10.1016\/j.bbrc.2008.11.039","article-title":"Structural basis for the recognition of lysozyme by MliC, a periplasmic lysozyme inhibitor in Gram-negative bacteria","volume":"378","author":"S Yum","year":"2009","journal-title":"Biochem Biophys Res Commun"},{"issue":"51","key":"ref12","doi-asserted-by":"crossref","first-page":"9385","DOI":"10.1021\/bi401241c","article-title":"Structural basis for the inhibition of human lysozyme by PliC from Brucella abortus","volume":"52","author":"SH Um","year":"2013","journal-title":"Biochemistry"},{"issue":"9","key":"ref13","doi-asserted-by":"crossref","first-page":"e45954","DOI":"10.1371\/journal.pone.0045954","article-title":"Role of lysozyme inhibitors in the virulence of avian pathogenic Escherichia coli","volume":"7","author":"L Vanderkelen","year":"2012","journal-title":"PloS one"},{"issue":"2","key":"ref14","doi-asserted-by":"crossref","first-page":"583","DOI":"10.1128\/IAI.02473-14","article-title":"Edwardsiella tarda MliC, a lysozyme inhibitor that participates in pathogenesis in a manner that parallels Ivy","volume":"83","author":"MF Li","year":"2015","journal-title":"Infection and immunity"},{"issue":"5","key":"ref15","doi-asserted-by":"crossref","first-page":"1299","DOI":"10.1074\/mcp.M113.029538","article-title":"Quantitative proteomics of the Neisseria gonorrhoeae cell envelope and membrane vesicles for the discovery of potential therapeutic targets","volume":"13","author":"RA Zielke","year":"2014","journal-title":"Mol Cell Proteomics"},{"key":"ref16","article-title":"Proteomics-driven antigen discovery for development of vaccines against gonorrhea","author":"RA Zielke","year":"2016","journal-title":"Mol Cell Proteomics"},{"key":"ref17","article-title":"The novel 2016 WHO Neisseria gonorrhoeae reference strains for global quality assurance of laboratory investigations: phenotypic, genetic and reference genome characterization","author":"M Unemo","year":"2016","journal-title":"The Journal of antimicrobial chemotherapy"},{"issue":"21","key":"ref18","doi-asserted-by":"crossref","first-page":"18437","DOI":"10.1074\/jbc.M010297200","article-title":"Escherichia coli ykfE ORFan gene encodes a potent inhibitor of C-type lysozyme","volume":"276","author":"V Monchois","year":"2001","journal-title":"The Journal of biological chemistry"},{"issue":"5","key":"ref19","doi-asserted-by":"crossref","first-page":"1233","DOI":"10.1016\/j.jmb.2010.12.007","article-title":"Molecular basis of bacterial defense against host lysozymes: X-ray structures of periplasmic lysozyme inhibitors PliI and PliC","volume":"405","author":"S Leysen","year":"2011","journal-title":"J Mol Biol"},{"issue":"18","key":"ref20","doi-asserted-by":"crossref","first-page":"4769","DOI":"10.1073\/pnas.1702248114","article-title":"Redefining the essential trafficking pathway for outer membrane lipoproteins","volume":"114","author":"M Grabowicz","year":"2017","journal-title":"Proc Natl Acad Sci U S A"},{"issue":"23","key":"ref21","doi-asserted-by":"crossref","first-page":"4247","DOI":"10.1111\/febs.12041","article-title":"Lipoproteins in bacteria: structures and biosynthetic pathways","volume":"279","author":"H Nakayama","year":"2012","journal-title":"FEBS J"},{"issue":"2","key":"ref22","doi-asserted-by":"crossref","DOI":"10.1093\/femspd\/ftx010","article-title":"Neisserial surface lipoproteins: structure, function and biogenesis","volume":"75","author":"Y Hooda","year":"2017","journal-title":"Pathogens and disease"},{"issue":"4","key":"ref23","doi-asserted-by":"crossref","first-page":"1106","DOI":"10.1074\/jbc.RA117.000437","article-title":"Structural and functional insights into the role of BamD and BamE within the beta-barrel assembly machinery in Neisseria gonorrhoeae","volume":"293","author":"AE Sikora","year":"2018","journal-title":"The Journal of biological chemistry"},{"issue":"8","key":"ref24","doi-asserted-by":"crossref","first-page":"791","DOI":"10.2174\/138620709789104915","article-title":"Label-free detection of biomolecular interactions using BioLayer interferometry for kinetic characterization","volume":"12","author":"J Concepcion","year":"2009","journal-title":"Comb Chem High Throughput Screen"},{"issue":"84","key":"ref25","first-page":"e51383","article-title":"Bio-layer interferometry for measuring kinetics of protein-protein interactions and allosteric ligand effects","author":"NB Shah","year":"2014","journal-title":"J Vis Exp"},{"issue":"3","key":"ref26","doi-asserted-by":"crossref","first-page":"178","DOI":"10.1038\/nrmicro2713","article-title":"A bacterial siren song: intimate interactions between Neisseria and neutrophils","volume":"10","author":"AK Criss","year":"2012","journal-title":"Nature reviews Microbiology"},{"issue":"3","key":"ref27","doi-asserted-by":"crossref","DOI":"10.1111\/cmi.12662","article-title":"Two lytic transglycosylases in Neisseria gonorrhoeae impart resistance to killing by lysozyme and human neutrophils","volume":"19","author":"SA Ragland","year":"2017","journal-title":"Cellular microbiology"},{"issue":"1679","key":"ref28","doi-asserted-by":"crossref","DOI":"10.1098\/rstb.2015.0030","article-title":"Outer membrane lipoprotein biogenesis: Lol is not the end","volume":"370","author":"A Konovalova","year":"2015","journal-title":"Philos Trans R Soc Lond B Biol Sci"},{"issue":"10","key":"ref29","doi-asserted-by":"crossref","first-page":"e0162878","DOI":"10.1371\/journal.pone.0162878","article-title":"Neisserial Heparin Binding Antigen (NHBA) Contributes to the Adhesion of Neisseria meningitidis to Human Epithelial Cells","volume":"11","author":"I Vacca","year":"2016","journal-title":"PloS one"},{"issue":"2","key":"ref30","article-title":"The adhesin complex protein (ACP) of Neisseria meningitidis is a new adhesin with vaccine potential","volume":"4","author":"MC Hung","year":"2013","journal-title":"mBio"},{"issue":"6","key":"ref31","doi-asserted-by":"crossref","first-page":"1979","DOI":"10.1128\/JB.01549-08","article-title":"The Rcs two-component system regulates expression of lysozyme inhibitors and is induced by exposure to lysozyme","volume":"191","author":"L Callewaert","year":"2009","journal-title":"Journal of bacteriology"},{"issue":"9","key":"ref32","doi-asserted-by":"crossref","first-page":"e1006512","DOI":"10.1371\/journal.ppat.1006512","article-title":"From bacterial killing to immune modulation: Recent insights into the functions of lysozyme","volume":"13","author":"SA Ragland","year":"2017","journal-title":"PLoS Pathog"},{"issue":"9","key":"ref33","doi-asserted-by":"crossref","first-page":"710","DOI":"10.1038\/nri1180","article-title":"Defensins: antimicrobial peptides of innate immunity","volume":"3","author":"T Ganz","year":"2003","journal-title":"Nat Rev Immunol"},{"issue":"6","key":"ref34","doi-asserted-by":"crossref","first-page":"2938","DOI":"10.1074\/jbc.M115.662593","article-title":"Lipoprotein LprI of Mycobacterium tuberculosis Acts as a Lysozyme Inhibitor","volume":"291","author":"D Sethi","year":"2016","journal-title":"The Journal of biological chemistry"},{"issue":"1","key":"ref35","doi-asserted-by":"crossref","first-page":"137","DOI":"10.1067\/mob.2002.123034","article-title":"Antimicrobial factors in the cervical mucus plug","volume":"187","author":"M Hein","year":"2002","journal-title":"American journal of obstetrics and gynecology"},{"issue":"3","key":"ref36","doi-asserted-by":"crossref","first-page":"561","DOI":"10.1067\/mob.2002.125280","article-title":"Antimicrobial components of vaginal fluid","volume":"187","author":"EV Valore","year":"2002","journal-title":"American journal of obstetrics and gynecology"},{"issue":"11","key":"ref37","doi-asserted-by":"crossref","first-page":"7560","DOI":"10.4049\/jimmunol.175.11.7560","article-title":"Cationic polypeptides are required for anti-HIV-1 activity of human vaginal fluid","volume":"175","author":"N Venkataraman","year":"2005","journal-title":"Journal of immunology"},{"issue":"4","key":"ref38","doi-asserted-by":"crossref","first-page":"1258","DOI":"10.1021\/pr0605419","article-title":"Comprehensive proteomic analysis of human cervical-vaginal fluid","volume":"6","author":"S Dasari","year":"2007","journal-title":"J Proteome Res"},{"issue":"2","key":"ref39","doi-asserted-by":"crossref","first-page":"227","DOI":"10.1111\/j.1365-2958.1988.tb00024.x","article-title":"Recombination among protein II genes of Neisseria gonorrhoeae generates new coding sequences and increases structural variability in the protein II family","volume":"2","author":"TD Connell","year":"1988","journal-title":"Molecular microbiology"},{"issue":"Suppl. 2","key":"ref40","first-page":"A111","article-title":"Phenotypic, genetic and genomic characterisation of the WHO Neisseria gonorrhoeae reference strains for quality assurance of laboratory investigations globally","volume":"91","author":"MG, D. Unemo","year":"2015","journal-title":"Sex Transm Infect"},{"issue":"8740","key":"ref41","doi-asserted-by":"crossref","first-page":"514","DOI":"10.1016\/0140-6736(91)91297-8","article-title":"Point mutation in meningococcal por A gene associated with increased endemic disease","volume":"337","author":"BT McGuinness","year":"1991","journal-title":"Lancet"},{"issue":"1","key":"ref42","doi-asserted-by":"crossref","first-page":"129","DOI":"10.1186\/s12866-015-0453-1","article-title":"The Neisseria gonorrhoeae Obg protein is an essential ribosome-associated GTPase and a potential drug target","volume":"15","author":"RA Zielke","year":"2015","journal-title":"BMC Microbiol"},{"key":"ref43","first-page":"1274","article-title":",","volume":"85","author":"DS Kellogg JR.","year":"1963","journal-title":"J Bacteriol"},{"key":"ref44","doi-asserted-by":"crossref","DOI":"10.1002\/9780471729259.mc04a01s8","article-title":"Laboratory maintenance of Neisseria gonorrhoeae","author":"JM Spence","year":"2008","journal-title":"Curr Protoc Microbiol"},{"issue":"12","key":"ref45","doi-asserted-by":"crossref","first-page":"5008","DOI":"10.1128\/IAI.64.12.5008-5014.1996","article-title":"Identification and purification of a hemoglobin-binding outer membrane protein from Neisseria gonorrhoeae","volume":"64","author":"CJ Chen","year":"1996","journal-title":"Infection and immunity"},{"issue":"7","key":"ref46","doi-asserted-by":"crossref","first-page":"4368","DOI":"10.1128\/IAI.68.7.4368-4369.2000","article-title":"Expression of AniA, the major anaerobically induced outer membrane protein of Neisseria gonorrhoeae, provides protection against killing by normal human sera","volume":"68","author":"JA Cardinale","year":"2000","journal-title":"Infection and immunity"},{"issue":"2","key":"ref47","doi-asserted-by":"crossref","first-page":"179","DOI":"10.1016\/0022-2836(80)90283-1","article-title":"Analysis of gene control signals by DNA fusion and cloning in Escherichia coli","volume":"138","author":"MJ Casadaban","year":"1980","journal-title":"J Mol Biol"},{"key":"ref48","doi-asserted-by":"crossref","DOI":"10.1002\/9780471729259.mc04a03s34","article-title":"Isolation of Cell Envelopes and Naturally Released Membrane Vesicles of Neisseria gonorrhoeae","volume":"34","author":"RA Zielke","year":"2014","journal-title":"Curr Protoc Microbiol"},{"issue":"5987","key":"ref49","doi-asserted-by":"crossref","first-page":"52","DOI":"10.1126\/science.1190719","article-title":"Creation of a bacterial cell controlled by a chemically synthesized genome","volume":"329","author":"DG Gibson","year":"2010","journal-title":"Science"},{"issue":"2","key":"ref50","doi-asserted-by":"crossref","first-page":"523","DOI":"10.1093\/genetics\/154.2.523","article-title":"A homologue of the recombination-dependent growth gene, rdgC, is involved in gonococcal pilin antigenic variation","volume":"154","author":"IJ Mehr","year":"2000","journal-title":"Genetics"},{"issue":"3","key":"ref51","doi-asserted-by":"crossref","first-page":"354","DOI":"10.1016\/j.jsb.2013.06.013","article-title":"A dodecameric ring-like structure of the N0 domain of the type II secretin from enterotoxigenic Escherichia coli","volume":"183","author":"KV Korotkov","year":"2013","journal-title":"Journal of structural biology"},{"issue":"1","key":"ref52","doi-asserted-by":"crossref","first-page":"113","DOI":"10.1016\/0022-2836(86)90385-2","article-title":"Use of bacteriophage T7 RNA polymerase to direct selective high-level expression of cloned genes","volume":"189","author":"FW Studier","year":"1986","journal-title":"J Mol Biol"},{"issue":"1","key":"ref53","doi-asserted-by":"crossref","first-page":"269","DOI":"10.1128\/JB.154.1.269-277.1983","article-title":"Morphological heterogeneity among Salmonella lipopolysaccharide chemotypes in silver-stained polyacrylamide gels","volume":"154","author":"PJ Hitchcock","year":"1983","journal-title":"Journal of bacteriology"},{"issue":"1","key":"ref54","doi-asserted-by":"crossref","first-page":"115","DOI":"10.1016\/0003-2697(82)90673-X","article-title":"A sensitive silver stain for detecting lipopolysaccharides in polyacrylamide gels","volume":"119","author":"CM Tsai","year":"1982","journal-title":"Anal Biochem"},{"issue":"45","key":"ref55","doi-asserted-by":"crossref","first-page":"5741","DOI":"10.1016\/j.vaccine.2008.08.020","article-title":"Local and humoral immune responses against primary and repeat Neisseria gonorrhoeae genital tract infections of 17beta-estradiol-treated mice","volume":"26","author":"W Song","year":"2008","journal-title":"Vaccine"},{"key":"ref56","article-title":"Functional and structural studies on the Neisseria gonorrhoeae GmhA, the first enzyme in the glycero-manno-heptose biosynthesis pathways, demonstrate a critical role in lipooligosaccharide synthesis and gonococcal viability","author":"IH Wierzbicki","year":"2017","journal-title":"Microbiologyopen"},{"issue":"6","key":"ref57","doi-asserted-by":"crossref","first-page":"1051","DOI":"10.1128\/JB.02329-14","article-title":"A metalloprotease secreted by the type II secretion system links Vibrio cholerae with collagen","volume":"197","author":"BR Park","year":"2015","journal-title":"Journal of bacteriology"},{"issue":"3","key":"ref58","first-page":"275","article-title":"The rapid generation of mutation data matrices from protein sequences","volume":"8","author":"DT Jones","year":"1992","journal-title":"Comput Appl Biosci"}],"container-title":["PLOS Pathogens"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/dx.plos.org\/10.1371\/journal.ppat.1007081","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,8,27]],"date-time":"2022-08-27T00:52:36Z","timestamp":1661561556000},"score":1,"resource":{"primary":{"URL":"https:\/\/dx.plos.org\/10.1371\/journal.ppat.1007081"}},"subtitle":[],"editor":[{"given":"Christoph","family":"Tang","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2018,7,5]]},"references-count":58,"journal-issue":{"issue":"7","published-online":{"date-parts":[[2018,7,5]]}},"URL":"https:\/\/doi.org\/10.1371\/journal.ppat.1007081","relation":{},"ISSN":["1553-7374"],"issn-type":[{"value":"1553-7374","type":"electronic"}],"subject":[],"published":{"date-parts":[[2018,7,5]]}}}