{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,27]],"date-time":"2026-09-27T20:15:12Z","timestamp":1790540112556,"version":"4.1.0"},"update-to":[{"DOI":"10.1371\/journal.ppat.1007315","type":"new_version","label":"New version","source":"publisher","updated":{"date-parts":[[2018,10,5]],"date-time":"2018-10-05T00:00:00Z","timestamp":1538697600000}}],"reference-count":91,"publisher":"Public Library of Science (PLoS)","issue":"9","license":[{"start":{"date-parts":[[2018,9,25]],"date-time":"2018-09-25T00:00:00Z","timestamp":1537833600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100004440","name":"Wellcome Trust","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100004440","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100000265","name":"Medical Research Council","doi-asserted-by":"publisher","award":["MR\/K008749\/1"],"award-info":[{"award-number":["MR\/K008749\/1"]}],"id":[{"id":"10.13039\/501100000265","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000865","name":"Bill and Melinda Gates Foundation","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100000865","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100000278","name":"Department for International Development","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100000278","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100011264","name":"FP7 People: Marie-Curie Actions","doi-asserted-by":"publisher","award":["172 290080"],"award-info":[{"award-number":["172 290080"]}],"id":[{"id":"10.13039\/100011264","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100007316","name":"Klaus Tschira Stiftung","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100007316","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["www.plospathogens.org"],"crossmark-restriction":false},"short-container-title":["PLoS Pathog"],"DOI":"10.1371\/journal.ppat.1007315","type":"journal-article","created":{"date-parts":[[2018,9,25]],"date-time":"2018-09-25T13:38:15Z","timestamp":1537882695000},"page":"e1007315","update-policy":"https:\/\/doi.org\/10.1371\/journal.ppat.corrections_policy","source":"Crossref","is-referenced-by-count":66,"title":["The trypanocidal benzoxaborole AN7973 inhibits trypanosome mRNA processing"],"prefix":"10.1371","volume":"14","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-8280-0778","authenticated-orcid":true,"given":"Daniela","family":"Begolo","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2663-4389","authenticated-orcid":true,"given":"Isabel M.","family":"Vincent","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9964-281X","authenticated-orcid":true,"given":"Federica","family":"Giordani","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ina","family":"P\u00f6hner","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4094-7154","authenticated-orcid":true,"given":"Michael J.","family":"Witty","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1005-1349","authenticated-orcid":true,"given":"Timothy G.","family":"Rowan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zakaria","family":"Bengaly","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kirsten","family":"Gillingwater","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yvonne","family":"Freund","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5951-8670","authenticated-orcid":true,"given":"Rebecca C.","family":"Wade","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michael P.","family":"Barrett","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6384-0731","authenticated-orcid":true,"given":"Christine","family":"Clayton","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"340","published-online":{"date-parts":[[2018,9,25]]},"reference":[{"issue":"10","key":"ref1","doi-asserted-by":"crossref","first-page":"1115","DOI":"10.1016\/j.ijpara.2009.02.013","article-title":"Models for <italic>Trypanosoma evansi<\/italic> (surra), its control and economic impact on small-hold livestock owners in the Philippines","volume":"39","author":"RJ Dobson","year":"2009","journal-title":"Int J Parasitol"},{"issue":"2","key":"ref2","doi-asserted-by":"crossref","first-page":"197","DOI":"10.1016\/j.prevetmed.2013.10.024","article-title":"Mapping the economic benefits to livestock keepers from intervening against bovine trypanosomosis in Eastern Africa","volume":"113","author":"AP Shaw","year":"2014","journal-title":"Preventive veterinary medicine"},{"issue":"14","key":"ref3","doi-asserted-by":"crossref","first-page":"1862","DOI":"10.1017\/S0031182016001268","article-title":"The animal trypanosomiases and their chemotherapy: a review","volume":"143","author":"F Giordani","year":"2016","journal-title":"Parasitology"},{"key":"ref4","first-page":"257","article-title":"Epidemiology of human African trypanosomiasis","volume":"6","author":"JR Franco","year":"2014","journal-title":"Clinical epidemiology"},{"key":"ref5","doi-asserted-by":"crossref","first-page":"e1859","DOI":"10.1371\/journal.pntd.0001859","article-title":"Estimating and Mapping the Population at Risk of Sleeping Sickness","volume":"6","author":"P Simarro","year":"2012","journal-title":"PLoS Negl Trop Dis"},{"key":"ref6","unstructured":"Dndi. The Drugs for Neglected Diseases initiative. Available from: <ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" ext-link-type=\"uri\" xlink:href=\"http:\/\/www.dndi.org\" xlink:type=\"simple\">www.dndi.org<\/ext-link>."},{"key":"ref7","doi-asserted-by":"crossref","first-page":"194176","DOI":"10.1155\/2013\/194176","article-title":"<italic>Trypanosoma evansi<\/italic> and surra: a review and perspectives on origin, history, distribution, taxonomy, morphology, hosts, and pathogenic effects","volume":"2013","author":"M Desquesnes","year":"2013","journal-title":"BioMed research international"},{"issue":"16","key":"ref8","doi-asserted-by":"crossref","first-page":"4462","DOI":"10.1016\/j.bmc.2014.04.065","article-title":"The unique chemistry of benzoxaboroles: current and emerging applications in biotechnology and therapeutic treatments","volume":"22","author":"CT Liu","year":"2014","journal-title":"Bioorganic & medicinal chemistry"},{"issue":"10","key":"ref9","doi-asserted-by":"crossref","first-page":"1439","DOI":"10.1517\/14656566.2014.921158","article-title":"Tavaborole for the treatment of onychomycosis","volume":"15","author":"BE Elewski","year":"2014","journal-title":"Expert opinion on pharmacotherapy"},{"issue":"5832","key":"ref10","doi-asserted-by":"crossref","first-page":"1759","DOI":"10.1126\/science.1142189","article-title":"An antifungal agent inhibits an aminoacyl-tRNA synthetase by trapping tRNA in the editing site","volume":"316","author":"FL Rock","year":"2007","journal-title":"Science (New York, NY)"},{"issue":"3","key":"ref11","doi-asserted-by":"crossref","first-page":"1394","DOI":"10.1128\/AAC.02058-12","article-title":"Discovery of a novel class of boron-based antibacterials with activity against gram-negative bacteria","volume":"57","author":"V Hernandez","year":"2013","journal-title":"Antimicrobial agents and chemotherapy"},{"issue":"10","key":"ref12","doi-asserted-by":"crossref","first-page":"6271","DOI":"10.1128\/AAC.01339-16","article-title":"Discovery of Novel Oral Protein Synthesis Inhibitors of Mycobacterium tuberculosis That Target Leucyl-tRNA Synthetase","volume":"60","author":"A Palencia","year":"2016","journal-title":"Antimicrobial agents and chemotherapy"},{"issue":"1","key":"ref13","doi-asserted-by":"crossref","first-page":"48","DOI":"10.1016\/j.ab.2012.08.024","article-title":"Time-dependent, reversible, oxaborole inhibition of Escherichia coli leucyl-tRNA synthetase measured with a continuous fluorescence assay","volume":"431","author":"AB Shapiro","year":"2012","journal-title":"Analytical biochemistry"},{"issue":"8","key":"ref14","doi-asserted-by":"crossref","first-page":"2129","DOI":"10.1016\/j.bmcl.2009.03.007","article-title":"Discovery and structure-activity study of a novel benzoxaborole anti-inflammatory agent (AN2728) for the potential topical treatment of psoriasis and atopic dermatitis","volume":"19","author":"T Akama","year":"2009","journal-title":"Bioorganic & medicinal chemistry letters"},{"issue":"3","key":"ref15","doi-asserted-by":"crossref","first-page":"413","DOI":"10.1124\/jpet.116.232819","article-title":"Treatment of Skin Inflammation with Benzoxaborole Phosphodiesterase Inhibitors: Selectivity, Cellular Activity, and Effect on Cytokines Associated with Skin Inflammation and Skin Architecture Changes","volume":"358","author":"C Dong","year":"2016","journal-title":"The Journal of pharmacology and experimental therapeutics"},{"issue":"19","key":"ref16","doi-asserted-by":"crossref","first-page":"3410","DOI":"10.1016\/j.febslet.2012.07.058","article-title":"Boron-based phosphodiesterase inhibitors show novel binding of boron to PDE4 bimetal center","volume":"586","author":"YR Freund","year":"2012","journal-title":"FEBS letters"},{"issue":"3","key":"ref17","doi-asserted-by":"crossref","first-page":"615","DOI":"10.1124\/jpet.113.207662","article-title":"Linking phenotype to kinase: identification of a novel benzoxaborole hinge-binding motif for kinase inhibition and development of high-potency rho kinase inhibitors","volume":"347","author":"T Akama","year":"2013","journal-title":"The Journal of pharmacology and experimental therapeutics"},{"issue":"10","key":"ref18","doi-asserted-by":"crossref","first-page":"4379","DOI":"10.1128\/AAC.00498-10","article-title":"Discovery of novel orally bioavailable oxaborole 6-carboxamides that demonstrate cure in a murine model of late-stage central nervous system african trypanosomiasis","volume":"54","author":"B Nare","year":"2010","journal-title":"Antimicrobial agents and chemotherapy"},{"issue":"4","key":"ref19","doi-asserted-by":"crossref","first-page":"165","DOI":"10.1021\/ml100013s","article-title":"Discovery of novel benzoxaborole-based potent antitrypanosomal agents","volume":"1","author":"D Ding","year":"2010","journal-title":"ACS medicinal chemistry letters"},{"issue":"6","key":"ref20","doi-asserted-by":"crossref","first-page":"e1151","DOI":"10.1371\/journal.pntd.0001151","article-title":"SCYX-7158, an orally-active benzoxaborole for the treatment of stage 2 human African trypanosomiasis","volume":"5","author":"RT Jacobs","year":"2011","journal-title":"PLoS Negl Trop Dis"},{"key":"ref21","article-title":"Identification of a 4-fluorobenzyl l-valinate amide benzoxaborole (AN11736) as a potential development candidate for the treatment of Animal African Trypanosomiasis (AAT)","author":"T Akama","year":"2017","journal-title":"Bioorganic & medicinal chemistry letters"},{"issue":"12","key":"ref22","doi-asserted-by":"crossref","first-page":"e0004299","DOI":"10.1371\/journal.pntd.0004299","article-title":"Genomic and Proteomic Studies on the Mode of Action of Oxaboroles against the African Trypanosome","volume":"9","author":"DC Jones","year":"2015","journal-title":"PLoS Negl Trop Dis"},{"issue":"2","key":"ref23","doi-asserted-by":"crossref","first-page":"e1006850","DOI":"10.1371\/journal.ppat.1006850","article-title":"Host-parasite co-metabolic activation of antitrypanosomal aminomethyl-benzoxaboroles","volume":"14","author":"N Zhang","year":"2018","journal-title":"Plos Pathogens"},{"issue":"3","key":"ref24","doi-asserted-by":"crossref","first-page":"1299","DOI":"10.1016\/j.bmcl.2011.12.096","article-title":"Benzoxaborole antimalarial agents. Part 2: Discovery of fluoro-substituted 7-(2-carboxyethyl)-1,3-dihydro-1-hydroxy-2,1-benzoxaboroles","volume":"22","author":"YK Zhang","year":"2012","journal-title":"Bioorganic & medicinal chemistry letters"},{"issue":"13","key":"ref25","doi-asserted-by":"crossref","first-page":"5889","DOI":"10.1021\/acs.jmedchem.7b00621","article-title":"Benzoxaborole Antimalarial Agents. Part 5. Lead Optimization of Novel Amide Pyrazinyloxy Benzoxaboroles and Identification of a Preclinical Candidate","volume":"60","author":"YK Zhang","year":"2017","journal-title":"Journal of medicinal chemistry"},{"issue":"3","key":"ref26","doi-asserted-by":"crossref","first-page":"385","DOI":"10.15252\/emmm.201607370","article-title":"Targeting Toxoplasma gondii CPSF3 as a new approach to control toxoplasmosis","volume":"9","author":"A Palencia","year":"2017","journal-title":"EMBO molecular medicine"},{"key":"ref27","doi-asserted-by":"crossref","first-page":"14574","DOI":"10.1038\/ncomms14574","article-title":"A potent antimalarial benzoxaborole targets a <italic>Plasmodium falciparum<\/italic> cleavage and polyadenylation specificity factor homologue","volume":"8","author":"E Sonoiki","year":"2017","journal-title":"Nature Comm"},{"issue":"7121","key":"ref28","doi-asserted-by":"crossref","first-page":"953","DOI":"10.1038\/nature05363","article-title":"Polyadenylation factor CPSF-73 is the pre-mRNA 3&apos;-end-processing endonuclease","volume":"444","author":"CR Mandel","year":"2006","journal-title":"Nature"},{"issue":"4","key":"ref29","doi-asserted-by":"crossref","first-page":"459","DOI":"10.2217\/fmb.11.20","article-title":"Trans-splicing in trypanosomes: machinery and its impact on the parasite transcriptome","volume":"6","author":"S. Michaeli","year":"2011","journal-title":"Future Microbiol"},{"key":"ref30","doi-asserted-by":"crossref","first-page":"3777","DOI":"10.1093\/nar\/12.9.3777","article-title":"Many trypanosome messenger RNAs share a common 5&apos; terminal sequence","volume":"12","author":"T De Lange","year":"1984","journal-title":"Nucleic Acids Res"},{"key":"ref31","doi-asserted-by":"crossref","first-page":"2387","DOI":"10.1002\/j.1460-2075.1984.tb02144.x","article-title":"Discontinuous synthesis of mRNA in trypanosomes","volume":"3","author":"J Kooter","year":"1984","journal-title":"EMBO J"},{"issue":"1","key":"ref32","doi-asserted-by":"crossref","first-page":"720","DOI":"10.1128\/MCB.13.1.720","article-title":"Temporal order of RNA-processing reactions in trypanosomes: rapid <italic>trans<\/italic> splicing precedes polyadenylation of newly synthesized tubulin transcripts","volume":"13","author":"E Ullu","year":"1993","journal-title":"Mol Cell Biol"},{"key":"ref33","doi-asserted-by":"crossref","first-page":"491","DOI":"10.1101\/gad.8.4.491","article-title":"A common pyrimidine-rich motif governs <italic>trans<\/italic>-splicing and polyadenylation of tubulin polycistronic pre-mRNA in trypanosomes","volume":"8","author":"KR Matthews","year":"1994","journal-title":"Genes Dev"},{"key":"ref34","doi-asserted-by":"crossref","first-page":"1359","DOI":"10.1093\/nar\/22.8.1359","article-title":"Control of polyadenylation and alternative splicing of transcripts from adjacent genes in a procyclin expression site: a dual role for polypyrimidine tracts in trypanosomes?","volume":"22","author":"E Vassella","year":"1994","journal-title":"Nucleic Acids Res"},{"key":"ref35","doi-asserted-by":"crossref","first-page":"7428","DOI":"10.1128\/MCB.14.11.7428","article-title":"Hierarchies of RNA processing signals in a trypanosome surface antigen mRNA precursor","volume":"14","author":"M Hug","year":"1994","journal-title":"Mol Cell Biol"},{"key":"ref36","doi-asserted-by":"crossref","first-page":"26870","DOI":"10.1074\/jbc.M302405200","article-title":"<italic>Tb<\/italic>CPSF30 depletion by RNA interference disrupts polycistronic RNA processing in <italic>Trypanosoma brucei<\/italic>","volume":"278","author":"EF Hendriks","year":"2003","journal-title":"J Biol Chem"},{"issue":"2","key":"ref37","doi-asserted-by":"crossref","first-page":"221","DOI":"10.1080\/15476286.2015.1130208","article-title":"The polyadenylation complex of <italic>Trypanosoma brucei<\/italic>: Characterization of the functional poly(A) polymerase","volume":"13","author":"H Koch","year":"2016","journal-title":"RNA Biol"},{"key":"ref38","doi-asserted-by":"crossref","first-page":"542","DOI":"10.1128\/EC.2.3.542-551.2003","article-title":"RNA polymerase I transcribes procyclin genes and variant surface glycoprotein gene expression sites in <italic>Trypanosoma brucei<\/italic>","volume":"2","author":"A Gunzl","year":"2003","journal-title":"Eukaryot Cell"},{"key":"ref39","doi-asserted-by":"crossref","first-page":"891","DOI":"10.1016\/0092-8674(83)90546-9","article-title":"Tandem repetition of the 5&apos; mini-exon of variant surface glycoprotein genes: a multiple promoter for VSG gene transcription?","volume":"34","author":"T de Lange","year":"1983","journal-title":"Cell"},{"issue":"16","key":"ref40","doi-asserted-by":"crossref","first-page":"7314","DOI":"10.1128\/MCB.25.16.7314-7322.2005","article-title":"Trypanosomal TBP functions with the multisubunit transcription factor tSNAP to direct spliced-leader RNA gene expression","volume":"25","author":"A Das","year":"2005","journal-title":"Mol Cell Biol"},{"issue":"6","key":"ref41","doi-asserted-by":"crossref","first-page":"1676","DOI":"10.1093\/nar\/gkl090","article-title":"A TFIIB-like protein is indispensable for spliced leader RNA gene transcription in Trypanosoma brucei","volume":"34","author":"B Schimanski","year":"2006","journal-title":"Nucleic Acids Res"},{"key":"ref42","doi-asserted-by":"crossref","first-page":"641","DOI":"10.1128\/EC.00411-06","article-title":"Spliced leader RNA gene transcription in <italic>Trypanosoma brucei<\/italic> requires transcription factor TFIIH","volume":"6","author":"JH Lee","year":"2007","journal-title":"Eukaryot Cell"},{"key":"ref43","doi-asserted-by":"crossref","first-page":"28994","DOI":"10.1074\/jbc.M004193200","article-title":"Cotranscriptional cap 4 formation on the <italic>Trypanosoma brucei<\/italic> spliced leader RNA","volume":"275","author":"G Mair","year":"2000","journal-title":"J Biol Chem"},{"key":"ref44","doi-asserted-by":"crossref","first-page":"8190","DOI":"10.1073\/pnas.84.23.8190","article-title":"Trypanosome mRNAs have unusual &quot;cap 4&quot; structures acquired by addition of a spliced leader","volume":"84","author":"K Perry","year":"1987","journal-title":"Proceedings of the National Academy of Sciences of the United States of America"},{"key":"ref45","doi-asserted-by":"crossref","first-page":"9861","DOI":"10.1093\/nar\/15.23.9861","article-title":"Direct analysis of the mini-exon donor RNA of <italic>Trypanosoma brucei<\/italic>: detection of a novel cap structure also present in messenger RNA","volume":"15","author":"M Freistadt","year":"1987","journal-title":"Nucleic Acids Res"},{"key":"ref46","doi-asserted-by":"crossref","first-page":"15071","DOI":"10.1016\/S0021-9258(18)68147-5","article-title":"Discontinuously synthesized mRNA from <italic>Trypanosoma brucei<\/italic> contains the highly methylated 5&apos; cap structure, m7GpppA*A*C(2&apos;-O)mU*A","volume":"263","author":"M Freistadt","year":"1988","journal-title":"J Biol Chem"},{"key":"ref47","doi-asserted-by":"crossref","first-page":"4844","DOI":"10.1128\/MCB.12.11.4844","article-title":"<italic>Trypanosoma brucei<\/italic> spliced-leader RNA methylations are required for <italic>trans<\/italic> splicing <italic>in vivo<\/italic>","volume":"12","author":"KP McNally","year":"1992","journal-title":"Mol Cell Biol"},{"key":"ref48","doi-asserted-by":"crossref","first-page":"10074","DOI":"10.1073\/pnas.88.22.10074","article-title":"Trans splicing in trypanosomes requires methylation of the 5&apos; end of the spliced leader RNA","volume":"88","author":"E Ullu","year":"1991","journal-title":"Proc Natl Acad Sci U S A"},{"key":"ref49","doi-asserted-by":"crossref","first-page":"1503","DOI":"10.1093\/nar\/gki298","article-title":"Developmentally regulated instability of the GPI-PLC mRNA is dependent on a short-lived protein factor","volume":"33","author":"H Webb","year":"2005","journal-title":"Nucleic Acids Res"},{"issue":"5","key":"ref50","doi-asserted-by":"crossref","first-page":"e0006450","DOI":"10.1371\/journal.pntd.0006450","article-title":"Benzoxaborole treatment perturbs S-adenosyl-L-methionine metabolism in <italic>Trypanosoma brucei<\/italic>","volume":"12","author":"PC Steketee","year":"2018","journal-title":"PLoS Negl Trop Dis"},{"key":"ref51","article-title":"Drug target identification using a trypanosome over-expression library","author":"D Begolo","year":"2014","journal-title":"Antimicrobial agents and chemotherapy"},{"key":"ref52","doi-asserted-by":"crossref","first-page":"3837","DOI":"10.1128\/MCB.8.9.3837","article-title":"Polycistronic transcripts in trypanosomes and their accumulation during heat shock: evidence for a precursor role in mRNA synthesis","volume":"8","author":"M Muhich","year":"1988","journal-title":"Mol Cell Biol"},{"key":"ref53","doi-asserted-by":"crossref","first-page":"2495","DOI":"10.1074\/jbc.M705782200","article-title":"Control and regulation of gene expression: quantitative analysis of the expression of phosphoglycerate kinase in bloodstream form <italic>Trypanosoma brucei<\/italic>","volume":"283","author":"J Haanstra","year":"2008","journal-title":"J Biol Chem"},{"key":"ref54","doi-asserted-by":"crossref","first-page":"137","DOI":"10.1016\/j.molbiopara.2008.12.009","article-title":"Functional characterization and protein\u2013protein interactions of trypanosome splicing factors U2AF35, U2AF65 and SF1","volume":"164","author":"M Vazquez","year":"2009","journal-title":"Mol Biochem Parasitol"},{"key":"ref55","doi-asserted-by":"crossref","first-page":"27982","DOI":"10.1074\/jbc.M109.095349","article-title":"Analysis of spliceosomal proteins in trypanosomatids reveals novel functions in mRNA processing","volume":"285","author":"I Tkacz","year":"2010","journal-title":"J Biol Chem"},{"key":"ref56","doi-asserted-by":"crossref","first-page":"1211","DOI":"10.1093\/nar\/gks1133","article-title":"Trypanosome CNOT10 is essential for the integrity of the NOT deadenylase complex and for degradation of many mRNAs","volume":"41","author":"V F\u00e4rber","year":"2013","journal-title":"Nucleic Acids Res"},{"issue":"12","key":"ref57","doi-asserted-by":"crossref","first-page":"4075","DOI":"10.1016\/S0021-9258(17)34682-3","article-title":"Sinefungin, a potent inhibitor of virion mRNA(guanine-7-)-methyltransferase, mRNA(nucleoside-2&apos;-)-methyltransferase, and viral multiplication","volume":"253","author":"CS Pugh","year":"1978","journal-title":"J Biol Chem"},{"key":"ref58","doi-asserted-by":"crossref","first-page":"99","DOI":"10.1016\/j.molbiopara.2010.03.016","article-title":"Processing of a reporter mRNA in trypanosomes is not coupled to transcription by RNA polymerase II","volume":"172","author":"M Stewart","year":"2010","journal-title":"Mol Biochem Parasitol"},{"key":"ref59","doi-asserted-by":"crossref","first-page":"3002","DOI":"10.1242\/jcs.031823","article-title":"Heat shock causes a decrease in polysomes and appearance of stress granules in trypanosomes independently of eIF2\u03b1 phosphorylation at threonine 169, the residue equivalent to serine 51 in mammalian eIF2\u03b1","volume":"121","author":"S Kramer","year":"2008","journal-title":"J Cell Sci"},{"key":"ref60","doi-asserted-by":"crossref","first-page":"608","DOI":"10.1006\/expr.1995.1156","article-title":"Heat shock does not affect the transcriptional efficiency of the hsp 70 genes of <italic>Trypanosoma brucei<\/italic>","volume":"81","author":"MG-S Lee","year":"1995","journal-title":"Exp Parasit"},{"issue":"11","key":"ref61","doi-asserted-by":"crossref","first-page":"3319","DOI":"10.1093\/nar\/18.11.3319","article-title":"Permeable trypanosome cells as a model system for transcription and trans-splicing","volume":"18","author":"E Ullu","year":"1990","journal-title":"Nucleic Acids Res"},{"issue":"11","key":"ref62","doi-asserted-by":"crossref","first-page":"3811","DOI":"10.1093\/nar\/gkp236","article-title":"Transcriptionally active TFIIH of the early-diverged eukaryote <italic>Trypanosoma brucei<\/italic> harbors two novel core subunits but not a cyclin-activating kinase complex","volume":"37","author":"JH Lee","year":"2009","journal-title":"Nucleic Acids Res"},{"issue":"2","key":"ref63","doi-asserted-by":"crossref","first-page":"263","DOI":"10.1002\/wrna.1207","article-title":"RNA in development: how ribonucleoprotein granules regulate the life cycles of pathogenic protozoa","volume":"5","author":"S. Kramer","year":"2014","journal-title":"Wiley interdisciplinary reviews RNA"},{"issue":"Pt 12","key":"ref64","doi-asserted-by":"crossref","first-page":"2896","DOI":"10.1242\/jcs.099275","article-title":"Inhibition of mRNA maturation in trypanosomes causes the formation of novel foci at the nuclear periphery containing cytoplasmic regulators of mRNA fate","volume":"125","author":"S Kramer","year":"2012","journal-title":"J Cell Sci"},{"issue":"5","key":"ref65","doi-asserted-by":"crossref","first-page":"885","DOI":"10.1111\/mmi.12910","article-title":"The spliceosomal PRP19 complex of trypanosomes","volume":"95","author":"DL Ambrosio","year":"2015","journal-title":"Mol Microbiol"},{"key":"ref66","doi-asserted-by":"crossref","first-page":"e02356","DOI":"10.1128\/AAC.02356-16","article-title":"<italic>In vitro<\/italic>, <italic>ex vivo<\/italic>, and <italic>in vivo<\/italic> activities of diamidines against <italic>Trypanosoma congolense<\/italic> and <italic>Trypanosoma vivax<\/italic>","volume":"61","author":"K Gillingwater","year":"2017","journal-title":"Antimicrob Agents Chemother"},{"key":"ref67","doi-asserted-by":"crossref","first-page":"e20836","DOI":"10.1371\/journal.pone.0020836","article-title":"Efficacy study of novel diamidine compounds in a <italic>Trypanosoma evansi<\/italic> goat model","volume":"6","author":"K Gillingwater","year":"2011","journal-title":"PLoS One"},{"issue":"3","key":"ref68","doi-asserted-by":"crossref","first-page":"171","DOI":"10.1016\/S0304-4017(01)00415-0","article-title":"Standardised tests in mice and cattle for the detection of drug resistance in tsetse-transmitted trypanosomes of African domestic cattle","volume":"97","author":"MC Eisler","year":"2001","journal-title":"Vet Parasitol"},{"key":"ref69","doi-asserted-by":"crossref","first-page":"2768","DOI":"10.1128\/AAC.00044-13","article-title":"Metabolomics guides rational development of a simplified cell culture medium for drug screening against <italic>Trypanosoma brucei<\/italic>","volume":"57","author":"D Creek","year":"2013","journal-title":"Antimicrobial agents and chemotherapy"},{"issue":"2","key":"ref70","doi-asserted-by":"crossref","first-page":"139","DOI":"10.1016\/S0001-706X(97)00079-X","article-title":"The Alamar Blue assay to determine drug sensitivity of African trypanosomes (<italic>T<\/italic>.<italic>b<\/italic>. <italic>rhodesiense<\/italic> and <italic>T<\/italic>.<italic>b<\/italic>. <italic>gambiense<\/italic>) <italic>in vitro<\/italic>","volume":"68","author":"B Raz","year":"1997","journal-title":"Acta Trop"},{"issue":"4","key":"ref71","doi-asserted-by":"crossref","first-page":"665","DOI":"10.4269\/ajtmh.2009.09-0015","article-title":"Development of an Alamar Blue viability assay in 384-well format for high throughput whole cell screening of <italic>Trypanosoma brucei brucei<\/italic> bloodstream form strain 427","volume":"81","author":"ML Sykes","year":"2009","journal-title":"Am J Trop Med Hyg"},{"key":"ref72","article-title":"Import of fructose bisphosphate aldolase into the glycosomes of Trypanosoma brucei","author":"CE Clayton","year":"1987","journal-title":"J Cell Biol"},{"key":"ref73","doi-asserted-by":"crossref","first-page":"408","DOI":"10.1038\/sj.embor.7400930","article-title":"Spliced-leader RNA silencing: a novel stress-induced mechanism in <italic>Trypanosoma brucei<\/italic>","volume":"8","author":"Y Lustig","year":"2007","journal-title":"EMBO Rep"},{"key":"ref74","doi-asserted-by":"crossref","first-page":"911","DOI":"10.1186\/1471-2164-15-911","article-title":"Extensive stage-regulation of translation revealed by ribosome profiling of <italic>Trypanosoma brucei<\/italic>","volume":"15","author":"BC Jensen","year":"2014","journal-title":"BMC Genomics"},{"key":"ref75","doi-asserted-by":"crossref","first-page":"306","DOI":"10.1186\/s12864-016-2624-3","article-title":"Integrative analysis of the <italic>Trypanosoma brucei<\/italic> gene expression cascade predicts differential regulation of mRNA processing and unusual control of ribosomal protein expression","volume":"17","author":"E Antwi","year":"2016","journal-title":"BMC Genomics"},{"key":"ref76","doi-asserted-by":"crossref","first-page":"2281","DOI":"10.1128\/AAC.02109-15","article-title":"Untargeted metabolomics to ascertain antibiotic modes of action","volume":"60","author":"I Vincent","year":"2016","journal-title":"Antimicrob Agents Chemother"},{"issue":"7","key":"ref77","doi-asserted-by":"crossref","first-page":"1048","DOI":"10.1093\/bioinformatics\/bts069","article-title":"IDEOM: an Excel interface for analysis of LC-MS-based metabolomics data","volume":"28","author":"DJ Creek","year":"2012","journal-title":"Bioinformatics"},{"key":"ref78","doi-asserted-by":"crossref","first-page":"76","DOI":"10.1016\/j.molbiopara.2008.05.006","article-title":"Single-locus targeting constructs for reliable regulated RNAi and transgene expression in <italic>Trypanosoma brucei<\/italic>","volume":"161","author":"S Alsford","year":"2008","journal-title":"Mol Biochem Parasitol"},{"key":"ref79","unstructured":"Maestro v11.1, Schr\u00f6dinger, LLC, New York, NY, 2017."},{"issue":"3","key":"ref80","doi-asserted-by":"crossref","first-page":"403","DOI":"10.1016\/S0022-2836(05)80360-2","article-title":"Basic local alignment search tool","volume":"215","author":"SF Altschul","year":"1990","journal-title":"J Mol Biol"},{"issue":"D1","key":"ref81","doi-asserted-by":"crossref","first-page":"D279","DOI":"10.1093\/nar\/gkv1344","article-title":"The Pfam protein families database: towards a more sustainable future","volume":"44","author":"RD Finn","year":"2016","journal-title":"Nucleic Acids Res"},{"issue":"4","key":"ref82","doi-asserted-by":"crossref","first-page":"704","DOI":"10.1002\/prot.20660","article-title":"Very fast empirical prediction and rationalization of protein pKa values","volume":"61","author":"H Li","year":"2005","journal-title":"Proteins"},{"issue":"1","key":"ref83","doi-asserted-by":"crossref","first-page":"281","DOI":"10.1021\/acs.jctc.5b00864","article-title":"OPLS3: A Force Field Providing Broad Coverage of Drug-like Small Molecules and Proteins","volume":"12","author":"E Harder","year":"2016","journal-title":"J Chem Theory Comput"},{"issue":"5","key":"ref84","doi-asserted-by":"crossref","first-page":"631","DOI":"10.1016\/S0959-440X(98)80156-5","article-title":"Validation of protein models derived from experiment","volume":"8","author":"RA Laskowski","year":"1998","journal-title":"Curr Opin Struct Biol"},{"key":"ref85","doi-asserted-by":"crossref","first-page":"W52","DOI":"10.1093\/nar\/gkl082","article-title":"NOMAD-Ref: visualization, deformation and refinement of macromolecular structures based on all-atom normal mode analysis","volume":"34","author":"E Lindahl","year":"2006","journal-title":"Nucleic Acids Res"},{"issue":"1","key":"ref86","doi-asserted-by":"crossref","first-page":"48","DOI":"10.1021\/ml200215j","article-title":"Ring Structure and Aromatic Substituent Effects on the pK a of the Benzoxaborole Pharmacophore","volume":"3","author":"JW Tomsho","year":"2012","journal-title":"ACS medicinal chemistry letters"},{"key":"ref87","doi-asserted-by":"crossref","first-page":"6705","DOI":"10.1016\/S0040-4039(99)01303-9","article-title":"Synthesis and structure of benzoboroxoles: novel organoboron heterocycles","volume":"40","author":"V Zhdankin","year":"1999","journal-title":"Tetrahedron Lett"},{"key":"ref88","unstructured":"Induced Fit Docking Protocol: Glide v7.4, Prime v4.7. Schr\u00f6dinger, LLC, New York, NY, 2017."},{"issue":"1","key":"ref89","doi-asserted-by":"crossref","first-page":"83","DOI":"10.1111\/j.1747-0285.2005.00327.x","article-title":"Use of an induced fit receptor structure in virtual screening","volume":"67","author":"W Sherman","year":"2006","journal-title":"Chem Biol Drug Des"},{"issue":"2","key":"ref90","doi-asserted-by":"crossref","first-page":"534","DOI":"10.1021\/jm050540c","article-title":"Novel procedure for modeling ligand\/receptor induced fit effects","volume":"49","author":"W Sherman","year":"2006","journal-title":"Journal of medicinal chemistry"},{"key":"ref91","doi-asserted-by":"crossref","first-page":"307","DOI":"10.1111\/mmi.12764","article-title":"Transcriptome-wide analysis of mRNA decay reveals complex degradation kinetics and suggests a role for co-transcriptional degradation in determining mRNA levels","volume":"94","author":"A Fadda","year":"2014","journal-title":"Mol Microbiol"}],"updated-by":[{"DOI":"10.1371\/journal.ppat.1007315","type":"new_version","label":"New version","source":"publisher","updated":{"date-parts":[[2018,10,5]],"date-time":"2018-10-05T00:00:00Z","timestamp":1538697600000}}],"container-title":["PLOS Pathogens"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/dx.plos.org\/10.1371\/journal.ppat.1007315","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,9,2]],"date-time":"2022-09-02T14:41:33Z","timestamp":1662129693000},"score":1,"resource":{"primary":{"URL":"https:\/\/dx.plos.org\/10.1371\/journal.ppat.1007315"}},"subtitle":[],"editor":[{"given":"Margaret A.","family":"Phillips","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2018,9,25]]},"references-count":91,"journal-issue":{"issue":"9","published-online":{"date-parts":[[2018,9,25]]}},"URL":"https:\/\/doi.org\/10.1371\/journal.ppat.1007315","relation":{"has-preprint":[{"id-type":"doi","id":"10.1101\/295550","asserted-by":"object"}]},"ISSN":["1553-7374"],"issn-type":[{"value":"1553-7374","type":"electronic"}],"subject":[],"published":{"date-parts":[[2018,9,25]]}}}