{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,6]],"date-time":"2026-05-06T09:23:38Z","timestamp":1778059418144,"version":"3.51.4"},"update-to":[{"DOI":"10.1371\/journal.ppat.1007322","type":"new_version","label":"New version","source":"publisher","updated":{"date-parts":[[2018,10,10]],"date-time":"2018-10-10T00:00:00Z","timestamp":1539129600000}}],"reference-count":37,"publisher":"Public Library of Science (PLoS)","issue":"9","license":[{"start":{"date-parts":[[2018,9,28]],"date-time":"2018-09-28T00:00:00Z","timestamp":1538092800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/publicdomain\/zero\/1.0\/"}],"funder":[{"DOI":"10.13039\/100000060","name":"National Institute of Allergy and Infectious Diseases","doi-asserted-by":"publisher","award":["1U19AI109740"],"award-info":[{"award-number":["1U19AI109740"]}],"id":[{"id":"10.13039\/100000060","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000060","name":"National Institute of Allergy and Infectious Diseases","doi-asserted-by":"publisher","award":["F31AI131452"],"award-info":[{"award-number":["F31AI131452"]}],"id":[{"id":"10.13039\/100000060","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["www.plospathogens.org"],"crossmark-restriction":false},"short-container-title":["PLoS Pathog"],"DOI":"10.1371\/journal.ppat.1007322","type":"journal-article","created":{"date-parts":[[2018,9,28]],"date-time":"2018-09-28T19:58:46Z","timestamp":1538164726000},"page":"e1007322","update-policy":"https:\/\/doi.org\/10.1371\/journal.ppat.corrections_policy","source":"Crossref","is-referenced-by-count":24,"title":["Critical role for cholesterol in Lassa fever virus entry identified by a novel small molecule inhibitor targeting the viral receptor LAMP1"],"prefix":"10.1371","volume":"14","author":[{"given":"May Kwang-Mei","family":"Wang","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tao","family":"Ren","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hu","family":"Liu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sun-Young","family":"Lim","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kyungae","family":"Lee","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9165-148X","authenticated-orcid":true,"given":"Anna","family":"Honko","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7239-9821","authenticated-orcid":true,"given":"Huanying","family":"Zhou","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9856-4183","authenticated-orcid":true,"given":"Julie","family":"Dyall","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lisa","family":"Hensley","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8072-6650","authenticated-orcid":true,"given":"Ashley K.","family":"Gartin","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0541-4862","authenticated-orcid":true,"given":"James M.","family":"Cunningham","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"340","published-online":{"date-parts":[[2018,9,28]]},"reference":[{"key":"ref1","first-page":"75","article-title":"Lassa fever","volume":"262","author":"JB McCormick","year":"2002","journal-title":"Curr Top Microbiol Immunol"},{"issue":"12","key":"ref2","doi-asserted-by":"crossref","first-page":"1971","DOI":"10.3201\/eid1212.060812","article-title":"Mastomys natalensis and Lassa fever, West Africa","volume":"12","author":"E Lecompte","year":"2006","journal-title":"Emerg Infect Dis"},{"issue":"5396","key":"ref3","doi-asserted-by":"crossref","first-page":"2079","DOI":"10.1126\/science.282.5396.2079","article-title":"Identification of alpha-dystroglycan as a receptor for lymphocytic choriomeningitis virus and Lassa fever virus","volume":"282","author":"W Cao","year":"1998","journal-title":"Science"},{"issue":"10","key":"ref4","doi-asserted-by":"crossref","first-page":"5979","DOI":"10.1128\/JVI.79.10.5979-5987.2005","article-title":"Characterization of the interaction of lassa fever virus with its cellular receptor alpha-dystroglycan","volume":"79","author":"S Kunz","year":"2005","journal-title":"J Virol"},{"issue":"14","key":"ref5","doi-asserted-by":"crossref","first-page":"6412","DOI":"10.1128\/JVI.00257-16","article-title":"Lassa Virus Cell Entry via Dystroglycan Involves an Unusual Pathway of Macropinocytosis","volume":"90","author":"J Oppliger","year":"2016","journal-title":"J Virol"},{"issue":"16","key":"ref6","doi-asserted-by":"crossref","DOI":"10.1128\/JVI.00093-18","article-title":"TIM-1 Mediates Dystroglycan-Independent Entry of Lassa Virus","volume":"92","author":"RB Brouillette","year":"2018","journal-title":"J Virol"},{"issue":"5","key":"ref7","doi-asserted-by":"crossref","DOI":"10.1128\/JVI.01613-17","article-title":"Axl Can Serve as Entry Factor for Lassa Virus Depending on the Functional Glycosylation of Dystroglycan","volume":"92","author":"C Fedeli","year":"2018","journal-title":"J Virol"},{"issue":"4","key":"ref8","doi-asserted-by":"crossref","first-page":"2067","DOI":"10.1128\/JVI.06451-11","article-title":"Identification of cell surface molecules involved in dystroglycan-independent Lassa virus cell entry","volume":"86","author":"M Shimojima","year":"2012","journal-title":"J Virol"},{"issue":"6191","key":"ref9","doi-asserted-by":"crossref","first-page":"1506","DOI":"10.1126\/science.1252480","article-title":"Virus entry. Lassa virus entry requires a trigger-induced receptor switch","volume":"344","author":"LT Jae","year":"2014","journal-title":"Science"},{"issue":"2","key":"ref10","doi-asserted-by":"crossref","first-page":"e1005418","DOI":"10.1371\/journal.ppat.1005418","article-title":"Acidic pH-Induced Conformations and LAMP1 Binding of the Lassa Virus Glycoprotein Spike","volume":"12","author":"S Li","year":"2016","journal-title":"PLoS Pathog"},{"issue":"15","key":"ref11","doi-asserted-by":"crossref","first-page":"7584","DOI":"10.1128\/JVI.00651-15","article-title":"Molecular Mechanism for LAMP1 Recognition by Lassa Virus","volume":"89","author":"H Cohen-Dvashi","year":"2015","journal-title":"J Virol"},{"issue":"22","key":"ref12","doi-asserted-by":"crossref","first-page":"10329","DOI":"10.1128\/JVI.01624-16","article-title":"Role of LAMP1 Binding and pH Sensing by the Spike Complex of Lassa Virus","volume":"90","author":"H Cohen-Dvashi","year":"2016","journal-title":"J Virol"},{"issue":"1","key":"ref13","doi-asserted-by":"crossref","DOI":"10.1128\/mBio.01818-17","article-title":"Lamp1 Increases the Efficiency of Lassa Virus Infection by Promoting Fusion in Less Acidic Endosomal Compartments","volume":"9","author":"CE Hulseberg","year":"2018","journal-title":"MBio"},{"issue":"10","key":"ref14","doi-asserted-by":"crossref","first-page":"2031","DOI":"10.3390\/v4102031","article-title":"Pathogenesis of Lassa fever","volume":"4","author":"NE Yun","year":"2012","journal-title":"Viruses"},{"key":"ref15","article-title":"Lysosomal membrane glycoproteins bind cholesterol and contribute to lysosomal cholesterol export","volume":"5","author":"J Li","year":"2016","journal-title":"Elife"},{"key":"ref16","doi-asserted-by":"crossref","first-page":"62","DOI":"10.1186\/1741-7007-10-62","article-title":"Crystal structure of the conserved domain of the DC lysosomal associated membrane protein: implications for the lysosomal glycocalyx","volume":"10","author":"S Wilke","year":"2012","journal-title":"BMC Biol"},{"issue":"3","key":"ref17","doi-asserted-by":"crossref","first-page":"489","DOI":"10.1016\/j.bbrc.2016.09.093","article-title":"Lysosome-associated membrane proteins-1 and -2 (LAMP-1 and LAMP-2) assemble via distinct modes","volume":"479","author":"K Terasawa","year":"2016","journal-title":"Biochem Biophys Res Commun"},{"issue":"12","key":"ref18","doi-asserted-by":"crossref","first-page":"1129","DOI":"10.1038\/nmeth.3652","article-title":"Thermal proteome profiling monitors ligand interactions with cellular membrane proteins","volume":"12","author":"FB Reinhard","year":"2015","journal-title":"Nat Methods"},{"issue":"3","key":"ref19","doi-asserted-by":"crossref","first-page":"259","DOI":"10.1038\/nmeth.2368","article-title":"Proteome-wide mapping of cholesterol-interacting proteins in mammalian cells","volume":"10","author":"JJ Hulce","year":"2013","journal-title":"Nat Methods"},{"issue":"4","key":"ref20","doi-asserted-by":"crossref","first-page":"679","DOI":"10.1016\/S0092-8674(00)00170-7","article-title":"Retroviral entry mediated by receptor priming and low pH triggering of an envelope glycoprotein","volume":"103","author":"W Mothes","year":"2000","journal-title":"Cell"},{"issue":"3","key":"ref21","doi-asserted-by":"crossref","first-page":"1403","DOI":"10.1128\/JVI.78.3.1403-1410.2004","article-title":"The mature avian leukosis virus subgroup A envelope glycoprotein is metastable, and refolding induced by the synergistic effects of receptor binding and low pH is coupled to infection","volume":"78","author":"JG Smith","year":"2004","journal-title":"J Virol"},{"issue":"4746","key":"ref22","doi-asserted-by":"crossref","first-page":"34","DOI":"10.1126\/science.3513311","article-title":"A receptor-mediated pathway for cholesterol homeostasis","volume":"232","author":"MS Brown","year":"1986","journal-title":"Science"},{"issue":"7364","key":"ref23","doi-asserted-by":"crossref","first-page":"344","DOI":"10.1038\/nature10380","article-title":"Small molecule inhibitors reveal Niemann-Pick C1 is essential for Ebola virus infection","volume":"477","author":"M Cote","year":"2011","journal-title":"Nature"},{"issue":"2","key":"ref24","doi-asserted-by":"crossref","first-page":"239","DOI":"10.1021\/ml300370k","article-title":"Inhibition of Ebola Virus Infection: Identification of Niemann-Pick C1 as the Target by Optimization of a Chemical Probe","volume":"4","author":"K Lee","year":"2013","journal-title":"ACS Med Chem Lett"},{"issue":"5","key":"ref25","doi-asserted-by":"crossref","first-page":"4272","DOI":"10.1128\/JVI.73.5.4272-4278.1999","article-title":"The cholesterol requirement for sindbis virus entry and exit and characterization of a spike protein region involved in cholesterol dependence","volume":"73","author":"YE Lu","year":"1999","journal-title":"J Virol"},{"issue":"1","key":"ref26","doi-asserted-by":"crossref","first-page":"91","DOI":"10.1083\/jcb.140.1.91","article-title":"A single point mutation controls the cholesterol dependence of Semliki Forest virus entry and exit","volume":"140","author":"M Vashishtha","year":"1998","journal-title":"J Cell Biol"},{"issue":"4","key":"ref27","doi-asserted-by":"crossref","first-page":"e00801","DOI":"10.1128\/mBio.00801-15","article-title":"Haploid Genetic Screen Reveals a Profound and Direct Dependence on Cholesterol for Hantavirus Membrane Fusion","volume":"6","author":"LM Kleinfelter","year":"2015","journal-title":"MBio"},{"issue":"Pt 1","key":"ref28","doi-asserted-by":"crossref","first-page":"210","DOI":"10.1099\/vir.0.006387-0","article-title":"Crimean-Congo hemorrhagic fever virus entry and replication is clathrin-, pH- and cholesterol-dependent","volume":"90","author":"M Simon","year":"2009","journal-title":"J Gen Virol"},{"issue":"2","key":"ref29","doi-asserted-by":"crossref","first-page":"249","DOI":"10.1111\/j.1462-5822.2008.01250.x","article-title":"Hepatitis B virus infection is dependent on cholesterol in the viral envelope","volume":"11","author":"CM Bremer","year":"2009","journal-title":"Cell Microbiol"},{"issue":"18","key":"ref30","doi-asserted-by":"crossref","first-page":"12692","DOI":"10.1074\/jbc.274.18.12692","article-title":"Normal lysosomal morphology and function in LAMP-1-deficient mice","volume":"274","author":"N Andrejewski","year":"1999","journal-title":"J Biol Chem"},{"key":"ref31","article-title":"Identification of Potent Ebola Virus Entry Inhibitors with Suitable Properties for in Vivo Studies","author":"H Liu","year":"2018","journal-title":"J Med Chem"},{"issue":"11","key":"ref32","doi-asserted-by":"crossref","first-page":"2281","DOI":"10.1038\/nprot.2013.143","article-title":"Genome engineering using the CRISPR-Cas9 system","volume":"8","author":"FA Ran","year":"2013","journal-title":"Nat Protoc"},{"key":"ref33","article-title":"Biochemical Basis for Increased Activity of Ebola Glycoprotein in the 2013\u201316 Epidemic","author":"MK Wang","year":"2017","journal-title":"Cell Host Microbe"},{"issue":"6","key":"ref34","doi-asserted-by":"crossref","first-page":"845","DOI":"10.1038\/nprot.2015.053","article-title":"The Phyre2 web portal for protein modeling, prediction and analysis","volume":"10","author":"LA Kelley","year":"2015","journal-title":"Nat Protoc"},{"issue":"Web Server issue","key":"ref35","doi-asserted-by":"crossref","first-page":"W270","DOI":"10.1093\/nar\/gkr366","article-title":"SwissDock, a protein-small molecule docking web service based on EADock DSS","volume":"39","author":"A Grosdidier","year":"2011","journal-title":"Nucleic Acids Res"},{"issue":"13","key":"ref36","doi-asserted-by":"crossref","first-page":"1605","DOI":"10.1002\/jcc.20084","article-title":"UCSF Chimera\u2014a visualization system for exploratory research and analysis","volume":"25","author":"EF Pettersen","year":"2004","journal-title":"J Comput Chem"},{"issue":"2","key":"ref37","doi-asserted-by":"crossref","first-page":"308","DOI":"10.1016\/j.celrep.2012.07.010","article-title":"Dispatched and scube mediate the efficient secretion of the cholesterol-modified hedgehog ligand","volume":"2","author":"H Tukachinsky","year":"2012","journal-title":"Cell Rep"}],"updated-by":[{"DOI":"10.1371\/journal.ppat.1007322","type":"new_version","label":"New version","source":"publisher","updated":{"date-parts":[[2018,10,10]],"date-time":"2018-10-10T00:00:00Z","timestamp":1539129600000}}],"container-title":["PLOS Pathogens"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/dx.plos.org\/10.1371\/journal.ppat.1007322","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2020,11,11]],"date-time":"2020-11-11T09:40:02Z","timestamp":1605087602000},"score":1,"resource":{"primary":{"URL":"https:\/\/dx.plos.org\/10.1371\/journal.ppat.1007322"}},"subtitle":[],"editor":[{"given":"Matthias Johannes","family":"Schnell","sequence":"first","affiliation":[],"role":[{"role":"editor","vocabulary":"crossref"}]}],"short-title":[],"issued":{"date-parts":[[2018,9,28]]},"references-count":37,"journal-issue":{"issue":"9","published-online":{"date-parts":[[2018,9,28]]}},"URL":"https:\/\/doi.org\/10.1371\/journal.ppat.1007322","relation":{"new_version":[{"id-type":"doi","id":"10.1371\/journal.ppat.1007322","asserted-by":"object"}]},"ISSN":["1553-7374"],"issn-type":[{"value":"1553-7374","type":"electronic"}],"subject":[],"published":{"date-parts":[[2018,9,28]]}}}