{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,24]],"date-time":"2026-09-24T18:30:48Z","timestamp":1790274648487,"version":"4.1.0"},"update-to":[{"DOI":"10.1371\/journal.ppat.1007652","type":"new_version","label":"New version","source":"publisher","updated":{"date-parts":[[2019,8,22]],"date-time":"2019-08-22T00:00:00Z","timestamp":1566432000000}}],"reference-count":131,"publisher":"Public Library of Science (PLoS)","issue":"8","license":[{"start":{"date-parts":[[2019,8,12]],"date-time":"2019-08-12T00:00:00Z","timestamp":1565568000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["www.plospathogens.org"],"crossmark-restriction":false},"short-container-title":["PLoS Pathog"],"DOI":"10.1371\/journal.ppat.1007652","type":"journal-article","created":{"date-parts":[[2019,8,12]],"date-time":"2019-08-12T13:37:16Z","timestamp":1565617036000},"page":"e1007652","update-policy":"https:\/\/doi.org\/10.1371\/journal.ppat.corrections_policy","source":"Crossref","is-referenced-by-count":69,"title":["Transposon-insertion sequencing screens unveil requirements for EHEC growth and intestinal colonization"],"prefix":"10.1371","volume":"15","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-1151-0108","authenticated-orcid":true,"given":"Alyson R.","family":"Warr","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Troy P.","family":"Hubbard","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Diana","family":"Munera","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7099-6347","authenticated-orcid":true,"given":"Carlos J.","family":"Blondel","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pia","family":"Abel zur Wiesch","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4041-6989","authenticated-orcid":true,"given":"S\u00f6ren","family":"Abel","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaoxue","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Brigid M.","family":"Davis","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Matthew K.","family":"Waldor","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"340","published-online":{"date-parts":[[2019,8,12]]},"reference":[{"key":"ppat.1007652.ref001","unstructured":"World Health Organization. Shiga toxin-producing Escherichia coli (STEC) and food: attribution, characterization, and monitoring. 2018. (Microbiological Risk Assessment Series). Report No.: 31."},{"issue":"15","key":"ppat.1007652.ref002","first-page":"1","article-title":"Enteropathogenic and enterohemorrhagic E. coli: ecology, pathogenesis, and evolution","volume":"3","author":"EL Hartland","year":"2015","journal-title":"Frontiers in Cellular and Infection Microbiology"},{"key":"ppat.1007652.ref003","first-page":"321","article-title":"Shiga Toxin\/Verocytotoxin-Producing Escherichia coli Infections: Practical Clinical Perspectives","author":"KT Davis","year":"2014","journal-title":"Enterohemorrhagic Escherichia coli and Other Shiga Toxin-Producing E coli"},{"issue":"8325","key":"ppat.1007652.ref004","doi-asserted-by":"crossref","first-page":"619","DOI":"10.1016\/S0140-6736(83)91795-6","article-title":"Sporadic Cases of Haemolytic-Uraemic Syndrome Associated with Faecal Cytotoxin-producing Escherichia coli in stools","volume":"321","author":"MA Karmali","year":"1983","journal-title":"The Lancet"},{"issue":"6","key":"ppat.1007652.ref005","doi-asserted-by":"crossref","first-page":"364","DOI":"10.1056\/NEJM199508103330608","article-title":"Escherichia coli O157:H7 and the Hemolytic\u2013Uremic Syndrome","volume":"333","author":"TG Boyce","year":"1995","journal-title":"New England Journal of Medicine"},{"issue":"12","key":"ppat.1007652.ref006","doi-asserted-by":"crossref","first-page":"681","DOI":"10.1056\/NEJM198303243081203","article-title":"Hemorrhagic colitis associated with a rare Escherichia coli serotype","volume":"308","author":"L Riley","year":"1983","journal-title":"New England Journal of Medicine"},{"issue":"6819","key":"ppat.1007652.ref007","doi-asserted-by":"crossref","first-page":"529","DOI":"10.1038\/35054089","article-title":"Genome sequence of enterohaemorrhagic Escherichia coli O157:H7","volume":"409","author":"NT Perna","year":"2001","journal-title":"Nature"},{"issue":"4","key":"ppat.1007652.ref008","doi-asserted-by":"crossref","DOI":"10.1128\/genomeA.00821-14","article-title":"A Gapless, Unambiguous Genome Sequence of the Enterohemorrhagic Escherichia coli O157:H7 Strain EDL933","volume":"2","author":"H Latif","year":"2014","journal-title":"Genome Announcements"},{"key":"ppat.1007652.ref009","doi-asserted-by":"crossref","first-page":"21","DOI":"10.1016\/B978-0-12-397048-0.00002-4","volume-title":"Escherichia coli","author":"JW Sahl","year":"2013","edition":"Second Edition"},{"key":"ppat.1007652.ref010","doi-asserted-by":"crossref","first-page":"45","DOI":"10.1016\/B978-0-12-397048-0.00003-6","volume-title":"Escherichia coli","author":"S Chattopadhyay","year":"2013","edition":"Second Edition"},{"issue":"20","key":"ppat.1007652.ref011","doi-asserted-by":"crossref","first-page":"6881","DOI":"10.1128\/JB.00619-08","article-title":"The pangenome structure of Escherichia coli: comparative genomic analysis of E. coli commensal and pathogenic isolates","volume":"190","author":"DA Rasko","year":"2008","journal-title":"J Bacteriol"},{"issue":"1","key":"ppat.1007652.ref012","doi-asserted-by":"crossref","first-page":"e1000344","DOI":"10.1371\/journal.pgen.1000344","article-title":"Organised Genome Dynamics in the Escherichia coli Species Results in Highly Diverse Adaptive Paths.","volume":"5","author":"M Touchon","year":"2009","journal-title":"PLOS Genetics"},{"issue":"4","key":"ppat.1007652.ref013","doi-asserted-by":"crossref","first-page":"708","DOI":"10.1007\/s00248-010-9717-3","article-title":"Comparison of 61 Sequenced Escherichia coli Genomes","volume":"60","author":"O Lukjancenko","year":"2010","journal-title":"Microb Ecol"},{"issue":"4","key":"ppat.1007652.ref014","doi-asserted-by":"crossref","first-page":"1072","DOI":"10.1093\/gbe\/evx064","article-title":"Core Genes Evolve Rapidly in the Long-Term Evolution Experiment with Escherichia coli","volume":"9","author":"R Maddamsetti","year":"2017","journal-title":"Genome Biol Evol"},{"issue":"10","key":"ppat.1007652.ref015","doi-asserted-by":"crossref","first-page":"3257","DOI":"10.1128\/JCM.01471-13","article-title":"Enterohemorrhagic Escherichia coli (EHEC) from Non-EHEC Strains Based on Detection of Various Combinations of Type III Effector Genes","volume":"51","author":"S Delannoy","year":"2013","journal-title":"Journal of Clinical Microbiology"},{"issue":"12","key":"ppat.1007652.ref016","doi-asserted-by":"crossref","first-page":"7129","DOI":"10.1128\/IAI.71.12.7129-7139.2003","article-title":"Critical Roles for stx2, eae, and tir in Enterohemorrhagic Escherichia coli-Induced Diarrhea and Intestinal Inflammation in Infant Rabbits","volume":"71","author":"JM Ritchie","year":"2003","journal-title":"Infection and Immunity"},{"issue":"90","key":"ppat.1007652.ref017","first-page":"1","article-title":"Enterohemorrhagic E. coli (EHEC) pathogenesis","volume":"2","author":"Y Nguyen","year":"2012","journal-title":"Frontiers in Cellular and Infection Microbiology"},{"issue":"6","key":"ppat.1007652.ref018","doi-asserted-by":"crossref","first-page":"1420","DOI":"10.1111\/j.1365-2958.2011.07661.x","article-title":"Enteropathogenic and enterohaemorrhagic Escherichia coli: even more subversive elements","volume":"80","author":"ARC Wong","year":"2011","journal-title":"Molecular Microbiology"},{"key":"ppat.1007652.ref019","article-title":"From ingestion to colonization: the influence of the host environment on regulation of the LEE encoded type III secretion system in enterohaemorrhagic Escherichia coli.","volume":"6","author":"JPR Connolly","year":"2015","journal-title":"Frontiers in Microbiology"},{"issue":"3","key":"ppat.1007652.ref020","doi-asserted-by":"crossref","first-page":"1418","DOI":"10.1172\/JCI116718","article-title":"The role of the eae gene of enterohemorrhagic Escherichia coli in intimate attachment in vitro and in a porcine model","volume":"92","author":"MS Donnenberg","year":"1993","journal-title":"Journal of Clinical Investigation"},{"issue":"5","key":"ppat.1007652.ref021","doi-asserted-by":"crossref","first-page":"2070","DOI":"10.1128\/IAI.63.5.2070-2074.1995","article-title":"Investigation of Enterohemorrhagic Escherichia coli O157:H7 Adherence Characteristics and Invasion Potential Reveals a New Attachment Pattern Shared by Intestinal E. coli","volume":"63","author":"ML Mckee","year":"1995","journal-title":"Infection and Immunity"},{"key":"ppat.1007652.ref022","doi-asserted-by":"crossref","first-page":"7","DOI":"10.1128\/IAI.63.9.3621-3627.1995","article-title":"The Role of the eaeA Gene in Diarrhea and Neurological Complications in a Gnotobiotic Piglet Model of Enterohemorrhagic Escherichia coli Infection","volume":"63","author":"S Tzipori","year":"1995","journal-title":"Infection and Immunity"},{"issue":"0","key":"ppat.1007652.ref023","doi-asserted-by":"crossref","first-page":"4560","DOI":"10.1128\/IAI.66.9.4560-4563.1998","article-title":"Escherichia coli O157:H7 Requires Intimin for Enteropathogenicity in Calves","volume":"66","author":"EA Dean-Nystrom","year":"1998","journal-title":"Infection and Immunity"},{"issue":"5","key":"ppat.1007652.ref024","doi-asserted-by":"crossref","first-page":"2704","DOI":"10.1128\/IAI.70.5.2704-2707.2002","article-title":"Intimin Facilitates Colonization by Escherichia coli O157:H7 in Adult Ruminants","volume":"70","author":"N Cornick","year":"2002","journal-title":"Infection and Immunity"},{"issue":"4","key":"ppat.1007652.ref025","doi-asserted-by":"crossref","first-page":"315","DOI":"10.4161\/viru.24318","article-title":"Modulation of the enterohemorrhagic E. coli virulence program through the human gastrointestinal tract","volume":"4","author":"D Barnett Foster","year":"2013","journal-title":"Virulence"},{"issue":"8","key":"ppat.1007652.ref026","doi-asserted-by":"crossref","first-page":"e1000553","DOI":"10.1371\/journal.ppat.1000553","article-title":"The QseC Adrenergic Signaling Cascade in Enterohemorrhagic E. coli (EHEC).","volume":"5","author":"DT Hughes","year":"2009","journal-title":"PLoS Pathogens"},{"issue":"15","key":"ppat.1007652.ref027","doi-asserted-by":"crossref","first-page":"8951","DOI":"10.1073\/pnas.1537100100","article-title":"Bacteria-host communication: The language of hormones","volume":"100","author":"V Sperandio","year":"2003","journal-title":"Proceedings of the National Academy of Sciences"},{"issue":"2","key":"ppat.1007652.ref028","doi-asserted-by":"crossref","first-page":"541","DOI":"10.1099\/mic.0.020420-0","article-title":"Secreted indole serves as a signal for expression of type III secretion system translocators in enterohaemorrhagic Escherichia coli O157:H7","volume":"155","author":"H Hirakawa","year":"2009","journal-title":"Microbiology"},{"key":"ppat.1007652.ref029","article-title":"Interkingdom Chemical Signaling in Enterohemorrhagic Escherichia coli O157:H7","author":"M Kendall","journal-title":"Microbial Endocrinology: Interkingdom Signaling in Infectious Disease and Health"},{"issue":"7","key":"ppat.1007652.ref030","doi-asserted-by":"crossref","first-page":"1771","DOI":"10.1128\/JB.01292-10","article-title":"Retrospective Application of Transposon-Directed Insertion Site Sequencing to a Library of Signature-Tagged Mini-Tn5Km2 Mutants of Escherichia coli O157:H7 Screened in Cattle","volume":"193","author":"SE Eckert","year":"2011","journal-title":"Journal of Bacteriology"},{"issue":"10","key":"ppat.1007652.ref031","doi-asserted-by":"crossref","first-page":"767","DOI":"10.1038\/nmeth.1377","article-title":"Tn-seq: high-throughput parallel sequencing for fitness and genetic interaction studies in microorganisms","volume":"6","author":"T van Opijnen","year":"2009","journal-title":"Nature Methods"},{"issue":"3","key":"ppat.1007652.ref032","doi-asserted-by":"crossref","first-page":"279","DOI":"10.1016\/j.chom.2009.08.003","article-title":"Identifying Genetic Determinants Needed to Establish a Human Gut Symbiont in Its Habitat.","volume":"6","author":"AL Goodman","year":"2009","journal-title":"Cell Host & Microbe"},{"issue":"38","key":"ppat.1007652.ref033","doi-asserted-by":"crossref","first-page":"16422","DOI":"10.1073\/pnas.0906627106","article-title":"Tracking insertion mutants within libraries by deep sequencing and a genome-wide screen for Haemophilus genes required in the lung","volume":"106","author":"JD Gawronski","year":"2009","journal-title":"Proceedings of the National Academy of Sciences"},{"issue":"12","key":"ppat.1007652.ref034","doi-asserted-by":"crossref","first-page":"2308","DOI":"10.1101\/gr.097097.109","article-title":"Simultaneous assay of every Salmonella Typhi gene using one million transposon mutants","volume":"19","author":"GC Langridge","year":"2009","journal-title":"Genome Research"},{"issue":"5","key":"ppat.1007652.ref035","doi-asserted-by":"crossref","first-page":"e2001390","DOI":"10.1371\/journal.pbio.2001390","article-title":"Metabolic and fitness determinants for in vitro growth and intestinal colonization of the bacterial pathogen Campylobacter jejuni","volume":"15","author":"B Gao","year":"2017","journal-title":"PLOS Biology"},{"issue":"9","key":"ppat.1007652.ref036","doi-asserted-by":"crossref","first-page":"e2002860","DOI":"10.1371\/journal.pbio.2002860","article-title":"Genome-wide identification of bacterial plant colonization genes","volume":"15","author":"BJ Cole","year":"2017","journal-title":"PLOS Biology"},{"key":"ppat.1007652.ref037","first-page":"200","article-title":"Genome-Wide Identification by Transposon Insertion Sequencing of Escherichia coli K1 Genes Essential for In Vitro Growth, Gastrointestinal Colonizing Capacity, and Survival in Serum","author":"AJ McCarthy","year":"2018","journal-title":"Journal of Bacteriology"},{"issue":"5","key":"ppat.1007652.ref038","doi-asserted-by":"crossref","DOI":"10.1128\/AEM.03028-16","article-title":"Salmonella Persistence in Tomatoes Requires a Distinct Set of Metabolic Functions Identified by Transposon Insertion Sequencing","volume":"83","author":"MH de Moraes","year":"2017","journal-title":"Applied and Environmental Microbiology"},{"issue":"6","key":"ppat.1007652.ref039","doi-asserted-by":"crossref","first-page":"e1006434","DOI":"10.1371\/journal.ppat.1006434","article-title":"Genome-wide transposon mutagenesis of Proteus mirabilis: Essential genes, fitness factors for catheter-associated urinary tract infection, and the impact of polymicrobial infection on fitness requirements","volume":"13","author":"CE Armbruster","year":"2017","journal-title":"PLOS Pathogens"},{"issue":"8","key":"ppat.1007652.ref040","doi-asserted-by":"crossref","first-page":"1808","DOI":"10.1080\/21505594.2017.1391446","article-title":"Peripheral blood vessels are a niche for blood-borne meningococci","volume":"8","author":"E Capel","year":"2017","journal-title":"Virulence"},{"issue":"6","key":"ppat.1007652.ref041","first-page":"13","article-title":"Novel Genes Required for the Fitness of Streptococcus pyogenes in Human Saliva","volume":"2","author":"L Zhu","year":"2017"},{"issue":"5","key":"ppat.1007652.ref042","doi-asserted-by":"crossref","DOI":"10.1128\/AEM.01913-17","article-title":"Interactions of Salmonella enterica Serovar Typhimurium and Pectobacterium carotovorum within a Tomato Soft Rot","volume":"84","author":"AS George","year":"2017","journal-title":"Applied and Environmental Microbiology"},{"key":"ppat.1007652.ref043","article-title":"A Metagenome-Wide Association Study and Arrayed Mutant Library Confirm Acetobacter Lipopolysaccharide Genes Are Necessary for Association with Drosophila melanogaster.","author":"KM White","year":"2018","journal-title":"G3: Genes|Genomes|Genetics"},{"issue":"7706","key":"ppat.1007652.ref044","doi-asserted-by":"crossref","first-page":"503","DOI":"10.1038\/s41586-018-0124-0","article-title":"Mutant phenotypes for thousands of bacterial genes of unknown function","volume":"557","author":"MN Price","year":"2018","journal-title":"Nature"},{"issue":"11","key":"ppat.1007652.ref045","doi-asserted-by":"crossref","first-page":"e1004782","DOI":"10.1371\/journal.pgen.1004782","article-title":"ARTIST: High-Resolution Genome-Wide Assessment of Fitness Using Transposon-Insertion Sequencing.","volume":"10","author":"JR Pritchard","year":"2014","journal-title":"PLoS Genetics"},{"issue":"2","key":"ppat.1007652.ref046","doi-asserted-by":"crossref","first-page":"119","DOI":"10.1038\/nrmicro.2015.7","article-title":"The design and analysis of transposon insertion sequencing experiments","volume":"14","author":"MC Chao","year":"2016","journal-title":"Nature Reviews Microbiology"},{"issue":"4","key":"ppat.1007652.ref047","doi-asserted-by":"crossref","DOI":"10.1128\/mBio.01351-16","article-title":"The Nucleoid Binding Protein H-NS Biases Genome-Wide Transposon Insertion Landscapes","volume":"7","author":"S Kimura","year":"2016","journal-title":"mBio"},{"issue":"1","key":"ppat.1007652.ref048","doi-asserted-by":"crossref","DOI":"10.1128\/mBio.02133-16","article-title":"Comprehensive Essentiality Analysis of the Mycobacterium tuberculosis Genome via Saturating Transposon Mutagenesis.","volume":"8","author":"MA DeJesus","year":"2017","journal-title":"mBio"},{"issue":"3","key":"ppat.1007652.ref049","doi-asserted-by":"crossref","first-page":"1466","DOI":"10.1128\/IAI.73.3.1466-1474.2005","article-title":"The Locus of Enterocyte Effacement-Encoded Effector Proteins All Promote Enterohemorrhagic Escherichia coli Pathogenicity in Infant Rabbits","volume":"73","author":"JM Ritchie","year":"2005","journal-title":"Infection and Immunity"},{"issue":"22","key":"ppat.1007652.ref050","doi-asserted-by":"crossref","first-page":"6283","DOI":"10.1073\/pnas.1601718113","article-title":"Genetic analysis of Vibrio parahaemolyticus intestinal colonization","volume":"113","author":"TP Hubbard","year":"2016","journal-title":"Proceedings of the National Academy of Sciences"},{"issue":"4","key":"ppat.1007652.ref051","doi-asserted-by":"crossref","first-page":"e1003456","DOI":"10.1371\/journal.pgen.1003456","article-title":"Comprehensive Assignment of Roles for Salmonella Typhimurium Genes in Intestinal Colonization of Food-Producing Animals.","volume":"9","author":"RR Chaudhuri","year":"2013","journal-title":"PLoS Genetics"},{"issue":"2","key":"ppat.1007652.ref052","doi-asserted-by":"crossref","first-page":"115","DOI":"10.2174\/138920210790886871","article-title":"DNA Transposons: Nature and Applications in Genomics.","volume":"11","author":"M Munoz-Lopez","year":"2010","journal-title":"Current Genomics"},{"issue":"D1","key":"ppat.1007652.ref053","doi-asserted-by":"crossref","first-page":"D457","DOI":"10.1093\/nar\/gkv1070","article-title":"KEGG as a reference resource for gene and protein annotation","volume":"44","author":"M Kanehisa","year":"2016","journal-title":"Nucleic Acids Research"},{"issue":"1","key":"ppat.1007652.ref054","doi-asserted-by":"crossref","first-page":"33","DOI":"10.1093\/nar\/28.1.33","article-title":"The COG database: a tool for genome-scale analysis of protein functions and evolution","volume":"28","author":"RL Tatusov","year":"2000","journal-title":"Nucleic Acids Research"},{"issue":"D1","key":"ppat.1007652.ref055","doi-asserted-by":"crossref","first-page":"D261","DOI":"10.1093\/nar\/gku1223","article-title":"Expanded microbial genome coverage and improved protein family annotation in the COG database","volume":"43","author":"MY Galperin","year":"2015","journal-title":"Nucleic Acids Research"},{"issue":"1","key":"ppat.1007652.ref056","doi-asserted-by":"crossref","first-page":"e00031","DOI":"10.1128\/mSphere.00031-18","article-title":"Genomewide Identification of Essential Genes and Fitness Determinants of Streptococcus mutans UA159","volume":"3","author":"RC Shields","year":"2018","journal-title":"mSphere"},{"issue":"D1","key":"ppat.1007652.ref057","doi-asserted-by":"crossref","first-page":"D574","DOI":"10.1093\/nar\/gkt1131","article-title":"DEG 10, an update of the database of essential genes that includes both protein-coding genes and noncoding genomic elements","volume":"42","author":"H Luo","year":"2014","journal-title":"Nucleic Acids Research"},{"issue":"5331","key":"ppat.1007652.ref058","doi-asserted-by":"crossref","first-page":"1453","DOI":"10.1126\/science.277.5331.1453","article-title":"The Complete Genome Sequence of Escherichia coli K-12","volume":"277","author":"F Blattner","year":"1997","journal-title":"Science"},{"key":"ppat.1007652.ref059","first-page":"2","article-title":"Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection.","author":"T Baba","year":"2006","journal-title":"Molecular Systems Biology"},{"key":"ppat.1007652.ref060","doi-asserted-by":"crossref","DOI":"10.1038\/msb4100174","article-title":"Construction of consecutive deletions of the Escherichia coli chromosome","volume":"3","author":"J Kato","year":"2007","journal-title":"Molecular Systems Biology"},{"key":"ppat.1007652.ref061","first-page":"5","article-title":"Update on the Keio collection of Escherichia coli single-gene deletion mutants","author":"N Yamamoto","year":"2009","journal-title":"Molecular Systems Biology"},{"issue":"6","key":"ppat.1007652.ref062","doi-asserted-by":"crossref","first-page":"547","DOI":"10.1016\/j.resmic.2005.11.014","article-title":"Non-essential KDO biosynthesis and new essential cell envelope biogenesis genes in the Escherichia coli yrbG\u2013yhbG locus","volume":"157","author":"P Sperandeo","year":"2006","journal-title":"Research in Microbiology"},{"issue":"1","key":"ppat.1007652.ref063","first-page":"18","volume":"9","author":"ECA Goodall","year":"2018","journal-title":"The Essential Genome of Escherichia coli K-12"},{"key":"ppat.1007652.ref064","doi-asserted-by":"crossref","first-page":"6A.6.1","DOI":"10.1002\/9780471729259.mc06a06s38","article-title":"Infant Rabbit Model for Diarrheal Diseases: Infant Rabbit Model for Diarrheal Diseases.","author":"S Abel","year":"2015","journal-title":"Current Protocols in Microbiology"},{"issue":"1","key":"ppat.1007652.ref065","doi-asserted-by":"crossref","DOI":"10.1128\/mBio.00047-10","article-title":"Back to the Future: Studying Cholera Pathogenesis Using Infant Rabbits","volume":"1","author":"JM Ritchie","year":"2010","journal-title":"mBio"},{"issue":"6","key":"ppat.1007652.ref066","doi-asserted-by":"crossref","first-page":"e1004823","DOI":"10.1371\/journal.ppat.1004823","article-title":"Analysis of Bottlenecks in Experimental Models of Infection","volume":"11","author":"S Abel","year":"2015","journal-title":"PLOS Pathogens"},{"issue":"1","key":"ppat.1007652.ref067","first-page":"13","article-title":"Unsupervised Learning Approach for Comparing Multiple Transposon Insertion Sequencing Studies","volume":"4","author":"TP Hubbard","year":"2019"},{"key":"ppat.1007652.ref068","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1155\/2015\/534738","article-title":"Locus of Enterocyte Effacement: A Pathogenicity Island Involved in the Virulence of Enteropathogenic and Enterohemorragic Escherichia coli Subjected to a Complex Network of Gene Regulation.","volume":"2015","author":"FM Franzin","year":"2015","journal-title":"BioMed Research International"},{"issue":"6","key":"ppat.1007652.ref069","doi-asserted-by":"crossref","first-page":"652","DOI":"10.1016\/j.chom.2013.11.001","article-title":"Tn-Seq Analysis of Vibrio cholerae Intestinal Colonization Reveals a Role for T6SS-Mediated Antibacterial Activity in the Host.","volume":"14","author":"Y Fu","year":"2013","journal-title":"Cell Host & Microbe"},{"issue":"6","key":"ppat.1007652.ref070","doi-asserted-by":"crossref","first-page":"e1004866","DOI":"10.1371\/journal.ppat.1004866","article-title":"Host Delivery of Favorite Meals for Intracellular Pathogens","volume":"11","author":"Y Abu Kwaik","year":"2015","journal-title":"PLOS Pathogens"},{"issue":"2","key":"ppat.1007652.ref071","doi-asserted-by":"crossref","first-page":"195","DOI":"10.1016\/j.chembiol.2016.12.015","article-title":"Carbon Sources Tune Antibiotic Susceptibility in Pseudomonas aeruginosa via Tricarboxylic Acid Cycle Control","volume":"24","author":"S Meylan","year":"2017","journal-title":"Cell Chemical Biology"},{"issue":"11","key":"ppat.1007652.ref072","doi-asserted-by":"crossref","first-page":"1423","DOI":"10.1016\/j.chembiol.2014.08.020","article-title":"Targeting Bacterial Central Metabolism for Drug Development.","volume":"21","author":"P Murima","year":"2014","journal-title":"Chemistry & Biology"},{"issue":"8","key":"ppat.1007652.ref073","doi-asserted-by":"crossref","first-page":"2166","DOI":"10.1093\/emboj\/17.8.2166","article-title":"A novel EspA-associated surface organelle of enteropathogenic Escherichia coli involved in protein translocation into epithelial cells","volume":"17","author":"S Knutton","year":"1998","journal-title":"The EMBO Journal"},{"key":"ppat.1007652.ref074","doi-asserted-by":"crossref","first-page":"9","DOI":"10.1128\/IAI.67.9.4834-4842.1999","article-title":"The EspD Protein of Enterohemorrhagic Escherichia coli Is Required for the Formation of Bacterial Surface Appendages and Is Incorporated in the Cytoplasmic Membranes of Target Cells","volume":"67","author":"AU Kresse","year":"1999","journal-title":"Infection and Immunity"},{"key":"ppat.1007652.ref075","doi-asserted-by":"crossref","first-page":"10","DOI":"10.1128\/JB.180.17.4370-4379.1998","article-title":"Pas, a Novel Protein Required for Protein Secretion and Attaching and Effacing Activities of Enterohemorrhagic Escherichia coli","volume":"180","author":"AU Kresse","year":"1998","journal-title":"Journal of Bacteriology"},{"issue":"10","key":"ppat.1007652.ref076","doi-asserted-by":"crossref","first-page":"5943","DOI":"10.1128\/IAI.68.10.5943-5952.2000","article-title":"Isolation and Characterization of Mini-Tn5Km2 Insertion Mutants of Enterohemorrhagic Escherichia coli O157:H7 Deficient in Adherence to Caco-2 Cells","volume":"68","author":"I Tatsuno","year":"2000","journal-title":"Infection and Immunity"},{"issue":"11","key":"ppat.1007652.ref077","doi-asserted-by":"crossref","first-page":"2481","DOI":"10.1128\/JB.00118-13","article-title":"EscE and EscG Are Cochaperones for the Type III Needle Protein EscF of Enteropathogenic Escherichia coli","volume":"195","author":"N Sal-Man","year":"2013","journal-title":"Journal of Bacteriology"},{"issue":"5","key":"ppat.1007652.ref078","doi-asserted-by":"crossref","first-page":"1233","DOI":"10.1046\/j.1365-2958.2003.03911.x","article-title":"Identification and characterization of NleA, a non-LEE-encoded type III translocated virulence factor of enterohaemorrhagic Escherichia coli O157:H7: Identification and characterization of NleA","volume":"51","author":"S Gruenheid","year":"2004","journal-title":"Molecular Microbiology"},{"issue":"9","key":"ppat.1007652.ref079","doi-asserted-by":"crossref","first-page":"e1005121","DOI":"10.1371\/journal.ppat.1005121","article-title":"Enteropathogenic Escherichia coli Uses NleA to Inhibit NLRP3 Inflammasome Activation","volume":"11","author":"H Yen","year":"2015","journal-title":"PLOS Pathogens"},{"issue":"7","key":"ppat.1007652.ref080","doi-asserted-by":"crossref","first-page":"1429","DOI":"10.1111\/j.1462-5822.2008.01136.x","article-title":"Subversion of actin dynamics by EspM effectors of attaching and effacing bacterial pathogens","volume":"10","author":"A Arbeloa","year":"2008","journal-title":"Cellular Microbiology"},{"issue":"4","key":"ppat.1007652.ref081","doi-asserted-by":"crossref","first-page":"489","DOI":"10.1111\/j.1462-5822.2009.01410.x","article-title":"EspM inhibits pedestal formation by enterohaemorrhagic Escherichia coli and enteropathogenic E. coli and disrupts the architecture of a polarized epithelial monolayer","volume":"12","author":"M Simovitch","year":"2010","journal-title":"Cellular Microbiology"},{"issue":"13","key":"ppat.1007652.ref082","doi-asserted-by":"crossref","first-page":"3534","DOI":"10.1128\/JB.00046-10","article-title":"N-Terminal Type III Secretion Signal of Enteropathogenic Escherichia coli Translocator Proteins","volume":"192","author":"D Munera","year":"2010","journal-title":"Journal of Bacteriology"},{"issue":"12","key":"ppat.1007652.ref083","doi-asserted-by":"crossref","first-page":"4086","DOI":"10.1128\/JB.187.12.4086-4094.2005","article-title":"ClpXP Protease Controls Expression of the Type III Protein Secretion System through Regulation of RpoS and GrlR Levels in Enterohemorrhagic Escherichia coli","volume":"187","author":"S Iyoda","year":"2005","journal-title":"Journal of Bacteriology"},{"issue":"1","key":"ppat.1007652.ref084","doi-asserted-by":"crossref","first-page":"59","DOI":"10.1016\/j.femsle.2005.09.020","article-title":"ClpXP controls the expression of LEE genes in enterohaemorrhagic Escherichia coli","volume":"253","author":"T Tomoyasu","year":"2005","journal-title":"FEMS Microbiology Letters"},{"issue":"4","key":"ppat.1007652.ref085","doi-asserted-by":"crossref","first-page":"1465","DOI":"10.1128\/IAI.01265-07","article-title":"Activation of the Cpx Envelope Stress Response Down-Regulates Expression of Several Locus of Enterocyte Effacement-Encoded Genes in Enteropathogenic Escherichia coli","volume":"76","author":"DM MacRitchie","year":"2008","journal-title":"Infection and Immunity"},{"issue":"5","key":"ppat.1007652.ref086","doi-asserted-by":"crossref","first-page":"1766","DOI":"10.1128\/IAI.05679-11","article-title":"DegP Is Involved in Cpx-Mediated Posttranscriptional Regulation of the Type III Secretion Apparatus in Enteropathogenic Escherichia coli","volume":"80","author":"DM MacRitchie","year":"2012","journal-title":"Infection and Immunity"},{"issue":"15","key":"ppat.1007652.ref087","doi-asserted-by":"crossref","first-page":"4799","DOI":"10.1128\/jb.173.15.4799-4813.1991","article-title":"Cloning, mapping, and characterization of the Escherichia coli prc gene, which is involved in C-terminal processing of penicillin-binding protein 3","volume":"173","author":"H Hara","year":"1991","journal-title":"Journal of Bacteriology"},{"issue":"6","key":"ppat.1007652.ref088","doi-asserted-by":"crossref","first-page":"1286","DOI":"10.1128\/JB.00827-13","article-title":"Salinity-Dependent Impacts of ProQ, Prc, and Spr Deficiencies on Escherichia coli Cell Structure","volume":"196","author":"CH Kerr","year":"2014","journal-title":"Journal of Bacteriology"},{"issue":"5","key":"ppat.1007652.ref089","doi-asserted-by":"crossref","first-page":"782","DOI":"10.1016\/j.bbapap.2008.12.015","article-title":"Drug transport mechanism of the AcrB efflux pump","volume":"1794","author":"KM Pos","year":"2009","journal-title":"Biochimica et Biophysica Acta (BBA)\u2014Proteins and Proteomic"},{"issue":"4","key":"ppat.1007652.ref090","doi-asserted-by":"crossref","first-page":"625","DOI":"10.1016\/j.femsre.2004.09.003","article-title":"The interaction between bacteria and bile","volume":"29","author":"M Begley","year":"2005","journal-title":"FEMS Microbiology Reviews"},{"issue":"10","key":"ppat.1007652.ref091","doi-asserted-by":"crossref","first-page":"3484","DOI":"10.1073\/pnas.86.10.3484","article-title":"OxyR, a positive regulator of hydrogen peroxide-inducible genes in Escherichia coli and Salmonella typhimurium, is homologous to a family of bacterial regulatory proteins","volume":"86","author":"MF Christman","year":"1989","journal-title":"Proceedings of the National Academy of Sciences"},{"issue":"4","key":"ppat.1007652.ref092","doi-asserted-by":"crossref","first-page":"238","DOI":"10.1016\/j.micres.2011.09.004","article-title":"The role of OxyR and SoxRS in oxidative stress survival in Shigella flexneri","volume":"167","author":"A Daugherty","year":"2012","journal-title":"Microbiological Research"},{"issue":"6","key":"ppat.1007652.ref093","doi-asserted-by":"crossref","first-page":"e1002107","DOI":"10.1371\/journal.pgen.1002107","article-title":"Trade-Off between Bile Resistance and Nutritional Competence Drives Escherichia coli Diversification in the Mouse Gut","volume":"7","author":"M De Paepe","year":"2011","journal-title":"PLoS Genetics"},{"issue":"1","key":"ppat.1007652.ref094","doi-asserted-by":"crossref","first-page":"843","DOI":"10.1146\/annurev-biochem-060614-034251","article-title":"The Twin-Arginine Protein Translocation Pathway","volume":"84","author":"BC Berks","year":"2015","journal-title":"Annual Review of Biochemistry"},{"key":"ppat.1007652.ref095","first-page":"215","volume-title":"Virulence Mechanisms of Bacterial Pathogens","author":"ER Green","year":"2016","edition":"Fifth Edition"},{"issue":"8","key":"ppat.1007652.ref096","doi-asserted-by":"crossref","first-page":"3493","DOI":"10.1128\/IAI.00090-10","article-title":"Salmonella enterica Serovar Enteritidis tatB and tatC Mutants Are Impaired in Caco-2 Cell Invasion In Vitro and Show Reduced Systemic Spread in Chickens","volume":"78","author":"CS Mickael","year":"2010","journal-title":"Infection and Immunity"},{"issue":"5","key":"ppat.1007652.ref097","doi-asserted-by":"crossref","first-page":"887","DOI":"10.1111\/mmi.12318","article-title":"Twin-arginine translocation system (tat) mutants of Salmonella are attenuated due to envelope defects, not respiratory defects: Role of Tat in Salmonella virulence","volume":"89","author":"M Craig","year":"2013","journal-title":"Molecular Microbiology"},{"issue":"10","key":"ppat.1007652.ref098","doi-asserted-by":"crossref","first-page":"e1007391","DOI":"10.1371\/journal.ppat.1007391","article-title":"Tat-exported peptidoglycan amidase-dependent cell division contributes to Salmonella Typhimurium fitness in the inflamed gut","volume":"14","author":"M Fujimoto","year":"2018","journal-title":"PLOS Pathogens"},{"issue":"4","key":"ppat.1007652.ref099","doi-asserted-by":"crossref","DOI":"10.1128\/IAI.00867-16","article-title":"The Tat Substrate SufI Is Critical for the Ability of Yersinia pseudotuberculosis To Cause Systemic Infection","volume":"85","author":"U Avican","year":"2017","journal-title":"Infection and Immunity"},{"issue":"3","key":"ppat.1007652.ref100","doi-asserted-by":"crossref","first-page":"1768","DOI":"10.1128\/IAI.74.3.1768-1776.2006","article-title":"The Twin Arginine Translocation System Is Essential for Virulence of Yersinia pseudotuberculosis","volume":"74","author":"M Lavander","year":"2006","journal-title":"Infection and Immunity"},{"issue":"8","key":"ppat.1007652.ref101","doi-asserted-by":"crossref","first-page":"935","DOI":"10.1089\/fpd.2009.0298","article-title":"Functional Characterization of the Twin-Arginine Translocation System in Campylobacter jejuni.","volume":"6","author":"G Rajashekara","year":"2009","journal-title":"Foodborne Pathogens and Disease"},{"issue":"1","key":"ppat.1007652.ref102","doi-asserted-by":"crossref","first-page":"114","DOI":"10.1186\/1471-2180-9-114","article-title":"Pleiotropic effects of the twin-arginine translocation system on biofilm formation, colonization, and virulence in Vibrio cholerae","volume":"9","author":"L Zhang","year":"2009","journal-title":"BMC Microbiology"},{"issue":"9","key":"ppat.1007652.ref103","doi-asserted-by":"crossref","first-page":"4908","DOI":"10.1128\/IAI.71.9.4908-4916.2003","article-title":"Contribution of the Twin Arginine Translocation System to the Virulence of Enterohemorrhagic Escherichia coli O157:H7","volume":"71","author":"N Pradel","year":"2003","journal-title":"Infection and Immunity"},{"issue":"22","key":"ppat.1007652.ref104","doi-asserted-by":"crossref","first-page":"8044","DOI":"10.1128\/JB.00773-07","article-title":"Role of SufI (FtsP) in Cell Division of Escherichia coli: Evidence for Its Involvement in Stabilizing the Assembly of the Divisome","volume":"189","author":"H Samaluru","year":"2007","journal-title":"Journal of Bacteriology"},{"issue":"2","key":"ppat.1007652.ref105","doi-asserted-by":"crossref","first-page":"504","DOI":"10.1016\/j.jmb.2008.12.043","article-title":"The Escherichia coli Cell Division Protein and Model Tat Substrate SufI (FtsP) Localizes to the Septal Ring and Has a Multicopper Oxidase-Like Structure","volume":"386","author":"M Tarry","year":"2009","journal-title":"Journal of Molecular Biology"},{"issue":"1","key":"ppat.1007652.ref106","doi-asserted-by":"crossref","first-page":"139","DOI":"10.1128\/JB.183.1.139-144.2001","article-title":"Escherichia coli Strains Blocked in Tat-Dependent Protein Export Exhibit Pleiotropic Defects in the Cell Envelope","volume":"183","author":"NR Stanley","year":"2001","journal-title":"Journal of Bacteriology"},{"issue":"5","key":"ppat.1007652.ref107","doi-asserted-by":"crossref","first-page":"1255","DOI":"10.1111\/j.1365-2958.2004.04063.x","article-title":"Screening for synthetic lethal mutants in Escherichia coli and identification of EnvC (YibP) as a periplasmic septal ring factor with murein hydrolase activity: E. coli synthetic lethal screen","volume":"52","author":"TG Bernhardt","year":"2004","journal-title":"Molecular Microbiology"},{"issue":"6","key":"ppat.1007652.ref108","doi-asserted-by":"crossref","first-page":"3158","DOI":"10.1128\/jb.171.6.3158-3161.1989","article-title":"Characterization of a purF operon mutation which affects colicin V production","volume":"171","author":"MJ Fath","year":"1989","journal-title":"Journal of Bacteriology"},{"issue":"22","key":"ppat.1007652.ref109","doi-asserted-by":"crossref","first-page":"6195","DOI":"10.1128\/JB.01012-12","article-title":"Transposon Mutagenesis Identifies Uropathogenic Escherichia coli Biofilm Factors","volume":"194","author":"M Hadjifrangiskou","year":"2012","journal-title":"Journal of Bacteriology"},{"key":"ppat.1007652.ref110","doi-asserted-by":"crossref","DOI":"10.3389\/fmed.2017.00163","article-title":"Interactions between Bacteria and Bile Salts in the Gastrointestinal and Hepatobiliary Tracts","volume":"4","author":"V Urdaneta","year":"2017","journal-title":"Frontiers in Medicine"},{"issue":"1","key":"ppat.1007652.ref111","doi-asserted-by":"crossref","DOI":"10.1128\/IAI.00471-16","article-title":"Purine Biosynthesis Metabolically Constrains Intracellular Survival of Uropathogenic Escherichia coli","volume":"85","author":"CL Shaffer","year":"2017","journal-title":"Infection and Immunity"},{"issue":"1","key":"ppat.1007652.ref112","doi-asserted-by":"crossref","first-page":"31","DOI":"10.1007\/s00253-015-7037-8","article-title":"Transposon sequencing: methods and expanding applications","volume":"100","author":"YM Kwon","year":"2016","journal-title":"Applied Microbiology and Biotechnology"},{"issue":"7","key":"ppat.1007652.ref113","doi-asserted-by":"crossref","first-page":"1161","DOI":"10.4161\/rna.24765","article-title":"Approaches to querying bacterial genomes with transposon-insertion sequencing","volume":"10","author":"L Barquist","year":"2013","journal-title":"RNA Biology"},{"key":"ppat.1007652.ref114","doi-asserted-by":"crossref","first-page":"8","DOI":"10.1128\/IAI.59.12.4310-4317.1991","article-title":"Construction of an eae Deletion Mutant of Enteropathogenic Escherichia coli by Using a Positive-Selection Suicide Vector","volume":"59","author":"MS Donnenberg","year":"1991","journal-title":"Infection and Immunity"},{"issue":"3","key":"ppat.1007652.ref115","doi-asserted-by":"crossref","first-page":"261","DOI":"10.2144\/000112242","article-title":"Gentamicin and other cassettes for chromosomal gene replacement in Escherichia coli","volume":"41","author":"AR Poteete","year":"2006","journal-title":"BioTechniques"},{"issue":"24","key":"ppat.1007652.ref116","doi-asserted-by":"crossref","first-page":"6418","DOI":"10.1128\/JB.00621-10","article-title":"Silent Mischief: Bacteriophage Mu Insertions Contaminate Products of Escherichia coli Random Mutagenesis Performed Using Suicidal Transposon Delivery Plasmids Mobilized by Broad-Host-Range RP4 Conjugative Machinery","volume":"192","author":"L Ferrieres","year":"2010","journal-title":"Journal of Bacteriology"},{"issue":"25","key":"ppat.1007652.ref117","doi-asserted-by":"crossref","first-page":"8736","DOI":"10.1073\/pnas.0803281105","article-title":"A defined transposon mutant library and its use in identifying motility genes in Vibrio cholerae","volume":"105","author":"DE Cameron","year":"2008","journal-title":"Proceedings of the National Academy of Sciences"},{"issue":"1\u20132","key":"ppat.1007652.ref118","doi-asserted-by":"crossref","first-page":"179","DOI":"10.1016\/S0378-1119(02)00856-9","article-title":"Construction of a mariner -based transposon for epitope-tagging and genomic targeting","volume":"296","author":"SL Chiang","year":"2002","journal-title":"Gene"},{"issue":"445","key":"ppat.1007652.ref119","doi-asserted-by":"crossref","first-page":"eaap8423","DOI":"10.1126\/scitranslmed.aap8423","article-title":"A live vaccine rapidly protects against cholera in an infant rabbit model","volume":"10","author":"TP Hubbard","year":"2018","journal-title":"Science Translational Medicine"},{"issue":"3","key":"ppat.1007652.ref120","doi-asserted-by":"crossref","first-page":"223","DOI":"10.1038\/nmeth.3253","article-title":"Sequence tag\u2013based analysis of microbial population dynamics","volume":"12","author":"S Abel","year":"2015","journal-title":"Nature Methods"},{"issue":"3","key":"ppat.1007652.ref121","doi-asserted-by":"crossref","DOI":"10.18637\/jss.v036.i03","article-title":"Conducting Meta-Analyses in R with the metafor Package","volume":"36","author":"W Viechtbauer","year":"2010","journal-title":"Journal of Statistical Software"},{"issue":"2","key":"ppat.1007652.ref122","doi-asserted-by":"crossref","first-page":"200","DOI":"10.1136\/gut.42.2.200","article-title":"Rapid modulation of electrolyte transport in Caco-2 cell monolayers by enteropathogenic Escherichia coli (EPEC) infection","volume":"42","author":"GK Collington","year":"1998","journal-title":"Gut"},{"issue":"17","key":"ppat.1007652.ref123","doi-asserted-by":"crossref","first-page":"7512","DOI":"10.1093\/nar\/gkr338","article-title":"A systems biology approach sheds new light on Escherichia coli acid resistance","volume":"39","author":"A Stincone","year":"2011","journal-title":"Nucleic Acids Research"},{"issue":"5","key":"ppat.1007652.ref124","doi-asserted-by":"crossref","first-page":"2115","DOI":"10.1128\/IAI.00012-14","article-title":"Differential Requirement for PBP1a and PBP1b in In Vivo and In Vitro Fitness of Vibrio cholerae","volume":"82","author":"T D\u00f6rr","year":"2014","journal-title":"Infection and Immunity"},{"issue":"16","key":"ppat.1007652.ref125","doi-asserted-by":"crossref","first-page":"E1610","DOI":"10.1073\/pnas.1401941111","article-title":"Bile salts act as effective protein-unfolding agents and instigators of disulfide stress in vivo","volume":"111","author":"CM Cremers","year":"2014","journal-title":"Proceedings of the National Academy of Sciences"},{"issue":"1","key":"ppat.1007652.ref126","doi-asserted-by":"crossref","first-page":"39","DOI":"10.1016\/0378-1119(91)90007-X","article-title":"A series of wide-host-range low-copy-number vectors that allow direct screening for recombinants","volume":"97","author":"VM Morales","year":"1991","journal-title":"Gene"},{"issue":"5","key":"ppat.1007652.ref127","doi-asserted-by":"crossref","first-page":"1027","DOI":"10.1016\/j.jmb.2004.03.016","article-title":"A Combined Transmembrane Topology and Signal Peptide Prediction Method","volume":"338","author":"L K\u00e4ll","year":"2004","journal-title":"Journal of Molecular Biology"},{"issue":"15","key":"ppat.1007652.ref128","doi-asserted-by":"crossref","first-page":"2237","DOI":"10.1016\/j.jmb.2017.12.007","article-title":"A Completely Reimplemented MPI Bioinformatics Toolkit with a New HHpred Server at its Core","volume":"430","author":"L Zimmermann","year":"2018","journal-title":"Journal of Molecular Biology"},{"issue":"6","key":"ppat.1007652.ref129","doi-asserted-by":"crossref","first-page":"845","DOI":"10.1038\/nprot.2015.053","article-title":"The Phyre2 web portal for protein modeling, prediction and analysis","volume":"10","author":"LA Kelley","year":"2015","journal-title":"Nature Protocols"},{"issue":"6","key":"ppat.1007652.ref130","doi-asserted-by":"crossref","first-page":"884","DOI":"10.1093\/bioinformatics\/btt607","article-title":"Protter: interactive protein feature visualization and integration with experimental proteomic data","volume":"30","author":"U Omasits","year":"2014","journal-title":"Bioinformatics"},{"issue":"22","key":"ppat.1007652.ref131","doi-asserted-by":"crossref","first-page":"2811","DOI":"10.1093\/bioinformatics\/btq530","article-title":"Combined prediction of Tat and Sec signal peptides with hidden Markov models","volume":"26","author":"PG Bagos","year":"2010","journal-title":"Bioinformatics"}],"updated-by":[{"DOI":"10.1371\/journal.ppat.1007652","type":"new_version","label":"New version","source":"publisher","updated":{"date-parts":[[2019,8,22]],"date-time":"2019-08-22T00:00:00Z","timestamp":1566432000000}}],"container-title":["PLOS Pathogens"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/dx.plos.org\/10.1371\/journal.ppat.1007652","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,1,16]],"date-time":"2021-01-16T09:23:02Z","timestamp":1610788982000},"score":1,"resource":{"primary":{"URL":"https:\/\/dx.plos.org\/10.1371\/journal.ppat.1007652"}},"subtitle":[],"editor":[{"given":"Vanessa","family":"Sperandio","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2019,8,12]]},"references-count":131,"journal-issue":{"issue":"8","published-online":{"date-parts":[[2019,8,12]]}},"URL":"https:\/\/doi.org\/10.1371\/journal.ppat.1007652","relation":{"has-review":[{"id-type":"doi","id":"10.3410\/f.736413515.793564769","asserted-by":"object"}],"has-preprint":[{"id-type":"doi","id":"10.1101\/563007","asserted-by":"object"}]},"ISSN":["1553-7374"],"issn-type":[{"value":"1553-7374","type":"electronic"}],"subject":[],"published":{"date-parts":[[2019,8,12]]}}}