{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,29]],"date-time":"2026-08-29T07:27:36Z","timestamp":1787988456086,"version":"build-2784847793"},"update-to":[{"DOI":"10.1371\/journal.ppat.1007818","type":"new_version","label":"New version","source":"publisher","updated":{"date-parts":[[2019,6,5]],"date-time":"2019-06-05T00:00:00Z","timestamp":1559692800000}}],"reference-count":45,"publisher":"Public Library of Science (PLoS)","issue":"5","license":[{"start":{"date-parts":[[2019,5,23]],"date-time":"2019-05-23T00:00:00Z","timestamp":1558569600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Ministry of Education","award":["2016R1D1A1A02937406"],"award-info":[{"award-number":["2016R1D1A1A02937406"]}]},{"DOI":"10.13039\/501100003653","name":"Korea National Institute of Health","doi-asserted-by":"publisher","award":["2013E-61002-00"],"award-info":[{"award-number":["2013E-61002-00"]}],"id":[{"id":"10.13039\/501100003653","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003052","name":"Ministry of Trade, Industry and Energy","doi-asserted-by":"publisher","award":["10067407"],"award-info":[{"award-number":["10067407"]}],"id":[{"id":"10.13039\/501100003052","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["www.plospathogens.org"],"crossmark-restriction":false},"short-container-title":["PLoS Pathog"],"DOI":"10.1371\/journal.ppat.1007818","type":"journal-article","created":{"date-parts":[[2019,5,23]],"date-time":"2019-05-23T18:49:00Z","timestamp":1558637340000},"page":"e1007818","update-policy":"https:\/\/doi.org\/10.1371\/journal.ppat.corrections_policy","source":"Crossref","is-referenced-by-count":43,"title":["Clonorchis sinensis excretory-secretory products increase malignant characteristics of cholangiocarcinoma cells in three-dimensional co-culture with biliary ductal plates"],"prefix":"10.1371","volume":"15","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-0491-8620","authenticated-orcid":true,"given":"Jihee","family":"Won","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1221-8261","authenticated-orcid":true,"given":"Youngkyu","family":"Cho","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dahyun","family":"Lee","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bo Young","family":"Jeon","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jung-Won","family":"Ju","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Seok","family":"Chung","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5104-5995","authenticated-orcid":true,"given":"Jhang Ho","family":"Pak","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"340","published-online":{"date-parts":[[2019,5,23]]},"reference":[{"key":"ref1","doi-asserted-by":"crossref","first-page":"630543","DOI":"10.1155\/2012\/630543","article-title":"Molecular pathogenesis of cholangiocarcinoma","volume":"2012","author":"G Fava","year":"2012","journal-title":"Int J Hepatol"},{"key":"ref2","doi-asserted-by":"crossref","first-page":"173","DOI":"10.1002\/hep.24351","article-title":"Risk factors for cholangiocarcinoma","volume":"54","author":"GL Tyson","year":"2011","journal-title":"Hepatology"},{"issue":"2","key":"ref3","doi-asserted-by":"crossref","first-page":"137","DOI":"10.1007\/s12029-011-9284-y","article-title":"Risk factors for gallbladder cancer and cholangiocarcinoma: similarities, differences and updates","volume":"43","author":"T Rustagi","year":"2012","journal-title":"J Gastrointest Cancer"},{"key":"ref4","doi-asserted-by":"crossref","first-page":"395","DOI":"10.1016\/j.pt.2012.07.006","article-title":"The tumorigenic liver fluke Opisthorchis viverrini\u2014multiple pathways to cancer","volume":"28","author":"B Sripa","year":"2012","journal-title":"Trends Parasitol"},{"key":"ref5","first-page":"1","article-title":"Biological agents. Volume 100 B. A review of human carcinogens","volume":"100","year":"2012","journal-title":"IARC Monogr Eval Carcinog Risks Hum"},{"issue":"11","key":"ref6","doi-asserted-by":"crossref","first-page":"590","DOI":"10.5483\/BMBRep.2016.49.11.109","article-title":"Clonorchis sinensis, an oriental liver fluke, as a human biological agent of cholangiocarcinoma: a brief review","volume":"49","author":"T-S Kim","year":"2016","journal-title":"BMB Rep"},{"key":"ref7","doi-asserted-by":"crossref","first-page":"773","DOI":"10.1016\/j.ijpara.2015.06.001","article-title":"Excretory\/secretory products of the carcinogenic liver fluke are endocytosed by human cholangiocytes and drive cell proliferation and IL6 production","volume":"45","author":"S Chaiyadet","year":"2015","journal-title":"Int J Parasitol"},{"key":"ref8","doi-asserted-by":"crossref","first-page":"616","DOI":"10.1016\/j.parint.2010.09.008","article-title":"Opisthorchis viverrini excretory\/secretory products induce toll-like receptor 4 upregulation and production of interleukin 6 and 8 in cholangiocyte","volume":"59","author":"K Ninlawan","year":"2010","journal-title":"Parasitol Int"},{"key":"ref9","doi-asserted-by":"crossref","first-page":"4447","DOI":"10.1007\/s00436-014-4127-y","article-title":"Induction of cancer-related microRNA expression profiling using excretory-secretory products of Clonorchis sinensis","volume":"113","author":"JH Pak","year":"2014","journal-title":"Parasitol Res"},{"key":"ref10","doi-asserted-by":"crossref","first-page":"145","DOI":"10.3389\/fonc.2018.00145","article-title":"Cell Adhesion and Matrix Stiffness: Coordinating Cancer Cell Invasion and Metastasis","volume":"8","author":"V Gkretsi","year":"2018","journal-title":"Frontiers in oncology"},{"issue":"9","key":"ref11","doi-asserted-by":"crossref","first-page":"455","DOI":"10.4254\/wjh.v9.i9.455","article-title":"Tumor reactive stroma in cholangiocarcinoma: The fuel behind cancer aggressiveness","volume":"9","author":"S Brivio","year":"2017","journal-title":"World journal of hepatology"},{"issue":"10","key":"ref12","doi-asserted-by":"crossref","first-page":"754","DOI":"10.1002\/jhbp.126","article-title":"Molecular mechanism of cholangiocarcinoma carcinogenesis","volume":"21","author":"K Maemura","year":"2014","journal-title":"Journal of Hepato-Biliary-Pancreatic Sciences"},{"key":"ref13","doi-asserted-by":"crossref","first-page":"1725","DOI":"10.1016\/j.ejca.2012.12.002","article-title":"Role of crosstalk between interleukin-6 and transforming growth factor-beta 1 in epithelial-mesenchymal transition and chemoresistance in biliary tract cancer","volume":"49","author":"D Yamada","year":"2013","journal-title":"Eur J Cancer"},{"key":"ref14","doi-asserted-by":"crossref","first-page":"e110705","DOI":"10.1371\/journal.pone.0110705","article-title":"Clonorchis sinensis infestation promotes three-dimensional aggregation and invasion of cholangiocarcinoma cells","volume":"9","author":"J Won","year":"2014","journal-title":"PLoS One"},{"key":"ref15","doi-asserted-by":"crossref","first-page":"2325","DOI":"10.1053\/j.gastro.2009.02.051","article-title":"Intrahepatic bile ducts develop according to a new mode of tubulogenesis regulated by the transcription factor SOX9","volume":"136","author":"A Antoniou","year":"2009","journal-title":"Gastroenterology"},{"key":"ref16","doi-asserted-by":"crossref","first-page":"1472","DOI":"10.1091\/mbc.e06-09-0848","article-title":"Liver progenitor cells develop cholangiocyte-type epithelial polarity in three-dimensional culture","volume":"18","author":"N Tanimizu","year":"2007","journal-title":"Mol Biol Cell"},{"key":"ref17","doi-asserted-by":"crossref","first-page":"214","DOI":"10.1115\/1.1449904","article-title":"Tensile mechanical properties of three-dimensional type I collagen extracellular matrices with varied microstructure","volume":"124","author":"BA Roeder","year":"2002","journal-title":"J Biomech Eng"},{"key":"ref18","doi-asserted-by":"crossref","first-page":"195","DOI":"10.1016\/j.ijpara.2015.11.003","article-title":"Oxidative stress-mediated mouse liver lesions caused by Clonorchis sinensis infection","volume":"46","author":"S Maeng","year":"2016","journal-title":"Int J Parasitol"},{"key":"ref19","doi-asserted-by":"crossref","first-page":"70","DOI":"10.1186\/s13071-015-0675-y","article-title":"The expression dynamics of transforming growth factor-\u03b2\/Smad signaling in the liver fibrosis experimentally caused by Clonorchis sinensis","volume":"8","author":"C Yan","year":"2015","journal-title":"Parasit Vectors"},{"key":"ref20","doi-asserted-by":"crossref","first-page":"684","DOI":"10.1038\/labinvest.2015.51","article-title":"Development and characterization of human-induced pluripotent stem cell-derived cholangiocytes","volume":"95","author":"TM De Assuncao","year":"2015","journal-title":"Lab Invest"},{"key":"ref21","doi-asserted-by":"crossref","first-page":"2","DOI":"10.1016\/S0168-8278(01)00078-2","article-title":"Ductular morphogenesis and functional polarization of normal human biliary epithelial cells in three-dimensional culture","volume":"35","author":"Y Ishida","year":"2001","journal-title":"J Hepatol"},{"key":"ref22","doi-asserted-by":"crossref","first-page":"2486","DOI":"10.1091\/mbc.e08-02-0177","article-title":"Liver progenitor cells fold up a cell monolayer into a double-layered structure during tubular morphogenesis","volume":"20","author":"N Tanimizu","year":"2009","journal-title":"Mol Biol Cell"},{"key":"ref23","first-page":"27","article-title":"Molecular Pathology of Liver Diseases","author":"Y Mizuguchi","year":"2011"},{"key":"ref24","doi-asserted-by":"crossref","first-page":"50","DOI":"10.1016\/j.biomaterials.2015.11.030","article-title":"Modelling the tumour microenvironment in long-term microencapsulated 3D co-cultures recapitulates phenotypic features of disease progression","volume":"78","author":"MF Estrada","year":"2016","journal-title":"Biomaterials"},{"key":"ref25","doi-asserted-by":"crossref","first-page":"2555","DOI":"10.1245\/s10434-009-0568-4","article-title":"Hepatic stellate cells may relate to progression of intrahepatic cholangiocarcinoma","volume":"16","author":"H Okabe","year":"2009","journal-title":"Ann Surg Oncol"},{"key":"ref26","doi-asserted-by":"crossref","first-page":"700","DOI":"10.1002\/hep.27165","article-title":"Generation of functional cholangiocyte-like cells from human pluripotent stem cells and HepaRG cells","volume":"60","author":"N Dianat","year":"2014","journal-title":"Hepatology"},{"key":"ref27","doi-asserted-by":"crossref","first-page":"218","DOI":"10.1111\/jcmm.12153","article-title":"The role of EGF-EGFR signalling pathway in hepatocellular carcinoma inflammatory microenvironment","volume":"18","author":"P Huang","year":"2014","journal-title":"J Cell Mol Med"},{"key":"ref28","doi-asserted-by":"crossref","first-page":"e0125793","DOI":"10.1371\/journal.pone.0125793","article-title":"Lipopolysaccharide (LPS)-induced biliary epithelial cell NRas activation requires epidermal growth factor receptor (EGFR)","volume":"10","author":"CE Trussoni","year":"2015","journal-title":"PLoS One"},{"key":"ref29","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1080\/15476278.2015.1019687","article-title":"Tissue stiffness dictates development, homeostasis, and disease progression","volume":"11","author":"AM Handorf","year":"2015","journal-title":"Organogenesis"},{"key":"ref30","doi-asserted-by":"crossref","first-page":"1394","DOI":"10.1002\/hep.22193","article-title":"The role of matrix stiffness in regulating cell behavior","volume":"47","author":"RG Wells","year":"2008","journal-title":"Hepatology"},{"key":"ref31","doi-asserted-by":"crossref","first-page":"899","DOI":"10.1056\/NEJM198404053101407","article-title":"Current concepts. Primary sclerosing cholangitis","volume":"310","author":"NF LaRusso","year":"1984","journal-title":"N Engl J Med"},{"key":"ref32","first-page":"259","article-title":"Ductular reaction and its diagnostic significance","volume":"15","author":"T Roskams","year":"1998","journal-title":"Semin Diagn Pathol"},{"key":"ref33","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.molbiopara.2017.03.002","article-title":"Clonorchis sinensis excretory-secretory products promote the migration and invasion of cholangiocarcinoma cells by activating the integrin beta4-FAK\/Src signaling pathway","volume":"214","author":"JH Pak","year":"2017","journal-title":"Mol Biochem Parasitol"},{"key":"ref34","doi-asserted-by":"crossref","first-page":"51","DOI":"10.1016\/j.ijpara.2016.10.004","article-title":"Clonorchis sinensis excretory-secretory products regulate migration and invasion in cholangiocarcinoma cells via extracellular signal-regulated kinase 1\/2\/nuclear factor-kappaB-dependent matrix metalloproteinase-9 expression","volume":"47","author":"JH Pak","year":"2017","journal-title":"Int J Parasitol"},{"key":"ref35","doi-asserted-by":"crossref","first-page":"149185","DOI":"10.1155\/2014\/149185","article-title":"Chronic inflammation and cytokines in the tumor microenvironment","volume":"2014","author":"G Landskron","year":"2014","journal-title":"J Immunol Res"},{"key":"ref36","doi-asserted-by":"crossref","first-page":"e1654","DOI":"10.1371\/journal.pntd.0001654","article-title":"Elevated Plasma IL-6 Associates with Increased Risk of Advanced Fibrosis and Cholangiocarcinoma in Individuals Infected by Opisthorchis viverrini","volume":"6","author":"B Sripa","year":"2012","journal-title":"PLoS Negl Trop Dis"},{"key":"ref37","doi-asserted-by":"crossref","first-page":"245","DOI":"10.1007\/s00441-011-1246-y","article-title":"TGF-\u03b2 in progression of liver disease","volume":"347","author":"S Dooley","year":"2012","journal-title":"Cell Tissue Res"},{"key":"ref38","doi-asserted-by":"crossref","first-page":"237","DOI":"10.1016\/j.cyto.2012.04.021","article-title":"Predominance of IL-10 and TGF-\u03b2 production from the mouse macrophage cell line, RAW264.7, in response to crude antigens from Clonorchis sinensis","volume":"59","author":"HJ Wi","year":"2012","journal-title":"Cytokine"},{"key":"ref39","doi-asserted-by":"crossref","first-page":"1885","DOI":"10.1038\/bjc.2011.452","article-title":"E\/N-cadherin switch mediates cancer progression via TGF-beta-induced epithelial-to-mesenchymal transition in extrahepatic cholangiocarcinoma","volume":"105","author":"K Araki","year":"2011","journal-title":"Br J Cancer"},{"issue":"10","key":"ref40","doi-asserted-by":"crossref","first-page":"9667","DOI":"10.1007\/s13277-014-2246-9","article-title":"BMP-7 blocks the effects of TGF-\u03b2-induced EMT in cholangiocarcinoma","volume":"35","author":"K Duangkumpha","year":"2014","journal-title":"Tumor Biology"},{"key":"ref41","doi-asserted-by":"crossref","first-page":"103","DOI":"10.1016\/j.ijpara.2011.11.001","article-title":"Free radicals enzymatically triggered by Clonorchis sinensis excretory-secretory products cause NF-kappaB-mediated inflammation in human cholangiocarcinoma cells","volume":"42","author":"JH Nam","year":"2012","journal-title":"Int J Parasitol"},{"key":"ref42","doi-asserted-by":"crossref","first-page":"503","DOI":"10.1007\/BF02623562","article-title":"A new human cholangiocellular carcinoma cell line (HuCC-T1) producing carbohydrate antigen 19\/9 in serum-free medium","volume":"25","author":"M Miyagiwa","year":"1989","journal-title":"In Vitro Cell Dev Biol"},{"key":"ref43","first-page":"G1060","article-title":"Regulation of intracellular pH by immortalized human intrahepatic biliary epithelial cell lines","volume":"266","author":"SA Grubman","year":"1994","journal-title":"Am J Physiol"},{"key":"ref44","doi-asserted-by":"crossref","first-page":"149","DOI":"10.1186\/1756-3305-4-149","article-title":"Molecular characterization of cathepsin B from Clonorchis sinensis excretory\/secretory products and assessment of its potential for serodiagnosis of clonorchiasis","volume":"4","author":"W Chen","year":"2011","journal-title":"Parasit Vectors"},{"key":"ref45","doi-asserted-by":"crossref","first-page":"717","DOI":"10.1007\/s10544-011-9541-7","article-title":"Microfluidic assay of endothelial cell migration in 3D interpenetrating polymer semi-network HA-Collagen hydrogel","volume":"13","author":"GS Jeong","year":"2011","journal-title":"Biomed Microdevices"}],"updated-by":[{"DOI":"10.1371\/journal.ppat.1007818","type":"new_version","label":"New version","source":"publisher","updated":{"date-parts":[[2019,6,5]],"date-time":"2019-06-05T00:00:00Z","timestamp":1559692800000}}],"container-title":["PLOS Pathogens"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/dx.plos.org\/10.1371\/journal.ppat.1007818","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2020,5,21]],"date-time":"2020-05-21T10:22:37Z","timestamp":1590056557000},"score":1,"resource":{"primary":{"URL":"https:\/\/dx.plos.org\/10.1371\/journal.ppat.1007818"}},"subtitle":[],"editor":[{"given":"Paul J.","family":"Brindley","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2019,5,23]]},"references-count":45,"journal-issue":{"issue":"5","published-online":{"date-parts":[[2019,5,23]]}},"URL":"https:\/\/doi.org\/10.1371\/journal.ppat.1007818","relation":{"new_version":[{"id-type":"doi","id":"10.1371\/journal.ppat.1007818","asserted-by":"object"}]},"ISSN":["1553-7374"],"issn-type":[{"value":"1553-7374","type":"electronic"}],"subject":[],"published":{"date-parts":[[2019,5,23]]}}}