{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,16]],"date-time":"2026-05-16T11:32:23Z","timestamp":1778931143956,"version":"3.51.4"},"update-to":[{"DOI":"10.1371\/journal.ppat.1007956","type":"new_version","label":"New version","source":"publisher","updated":{"date-parts":[[2019,10,17]],"date-time":"2019-10-17T00:00:00Z","timestamp":1571270400000}}],"reference-count":45,"publisher":"Public Library of Science (PLoS)","issue":"10","license":[{"start":{"date-parts":[[2019,10,7]],"date-time":"2019-10-07T00:00:00Z","timestamp":1570406400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100000265","name":"Medical Research Council","doi-asserted-by":"publisher","award":["MR\/L000148\/.1"],"award-info":[{"award-number":["MR\/L000148\/.1"]}],"id":[{"id":"10.13039\/501100000265","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100004440","name":"Wellcome Trust","doi-asserted-by":"publisher","award":["105395\/Z\/14\/Z"],"award-info":[{"award-number":["105395\/Z\/14\/Z"]}],"id":[{"id":"10.13039\/100004440","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100000761","name":"Imperial College London","doi-asserted-by":"publisher","award":["PS3314"],"award-info":[{"award-number":["PS3314"]}],"id":[{"id":"10.13039\/501100000761","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100000761","name":"Imperial College London","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100000761","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["www.plospathogens.org"],"crossmark-restriction":false},"short-container-title":["PLoS Pathog"],"DOI":"10.1371\/journal.ppat.1007956","type":"journal-article","created":{"date-parts":[[2019,10,7]],"date-time":"2019-10-07T13:30:31Z","timestamp":1570455031000},"page":"e1007956","update-policy":"https:\/\/doi.org\/10.1371\/journal.ppat.corrections_policy","source":"Crossref","is-referenced-by-count":8,"title":["Analysis of a fully infectious bio-orthogonally modified human virus reveals novel features of virus cell entry"],"prefix":"10.1371","volume":"15","author":[{"given":"Remigiusz A.","family":"Serwa","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6555-7879","authenticated-orcid":true,"given":"Eiki","family":"Sekine","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jonathan","family":"Brown","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1758-9515","authenticated-orcid":true,"given":"Su Hui Catherine","family":"Teo","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Edward W.","family":"Tate","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4508-5602","authenticated-orcid":true,"given":"Peter","family":"O\u2019Hare","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"340","published-online":{"date-parts":[[2019,10,7]]},"reference":[{"issue":"44","key":"ppat.1007956.ref001","doi-asserted-by":"crossref","first-page":"7364","DOI":"10.1002\/anie.200602114","article-title":"Fluorescence visualization of newly synthesized proteins in mammalian cells","volume":"45","author":"KE Beatty","year":"2006","journal-title":"Angew Chem Int Ed Engl"},{"issue":"3","key":"ppat.1007956.ref002","doi-asserted-by":"crossref","first-page":"532","DOI":"10.1038\/nprot.2007.52","article-title":"Labeling, detection and identification of newly synthesized proteomes with bioorthogonal non-canonical amino-acid tagging","volume":"2","author":"DC Dieterich","year":"2007","journal-title":"Nat Protoc"},{"issue":"11","key":"ppat.1007956.ref003","doi-asserted-by":"crossref","first-page":"2004","DOI":"10.1002\/1521-3773(20010601)40:11<2004::AID-ANIE2004>3.0.CO;2-5","article-title":"Click chemistry: Diverse chemical function from a few good reactions","volume":"40","author":"HC Kolb","year":"2001","journal-title":"Angewandte Chemie-International Edition"},{"issue":"14","key":"ppat.1007956.ref004","doi-asserted-by":"crossref","first-page":"2596","DOI":"10.1002\/1521-3773(20020715)41:14<2596::AID-ANIE2596>3.0.CO;2-4","article-title":"A stepwise Huisgen cycloaddition process: Copper(I)-catalyzed regioselective \"ligation\" of azides and terminal alkynes","volume":"41","author":"VV Rostovtsev","year":"2002","journal-title":"Angewandte Chemie-International Edition"},{"issue":"11","key":"ppat.1007956.ref005","first-page":"1","article-title":"Metabolic labelling with noncanonical amino acids and visualisation by Chemoselective fluorescent tagging","volume":"7","author":"S tom Dieck","year":"2012","journal-title":"Curr Protoc Cell Biol"},{"issue":"1","key":"ppat.1007956.ref006","doi-asserted-by":"crossref","first-page":"40","DOI":"10.1021\/cn2000876","article-title":"Noncanonical Amino Acid Labeling in Vivo to Visualize and Affinity Purify Newly Synthesized Proteins in Larval Zebrafish","volume":"3","author":"FI Hinz","year":"2012","journal-title":"Acs Chemical Neuroscience"},{"key":"ppat.1007956.ref007","article-title":"Cell-selective metabolic labeling of proteins","volume":"239","author":"DA Tirrell","year":"2010","journal-title":"Abstracts of Papers of the American Chemical Society"},{"issue":"7","key":"ppat.1007956.ref008","doi-asserted-by":"crossref","first-page":"897","DOI":"10.1038\/nn.2580","article-title":"In situ visualization and dynamics of newly synthesized proteins in rat hippocampal neurons","volume":"13","author":"DC Dieterich","year":"2010","journal-title":"Nature Neuroscience"},{"issue":"15","key":"ppat.1007956.ref009","doi-asserted-by":"crossref","first-page":"2092","DOI":"10.1002\/cbic.201000419","article-title":"Live-Cell Imaging of Cellular Proteins by a Strain-Promoted Azide-Alkyne Cycloaddition","volume":"11","author":"KE Beatty","year":"2010","journal-title":"Chembiochem"},{"issue":"11","key":"ppat.1007956.ref010","doi-asserted-by":"crossref","first-page":"4815","DOI":"10.1021\/acs.jproteome.5b00653","article-title":"Pulsed Azidohomoalanine Labeling in Mammals (PALM) Detects Changes in Liver-Specific LKB1 Knockout Mice","volume":"14","author":"DB McClatchy","year":"2015","journal-title":"J Proteome Res"},{"issue":"11","key":"ppat.1007956.ref011","doi-asserted-by":"crossref","first-page":"e1006721","DOI":"10.1371\/journal.ppat.1006721","article-title":"Spatiotemporal dynamics of HSV genome nuclear entry and compaction state transitions using bioorthogonal chemistry and super-resolution microscopy","volume":"13","author":"E Sekine","year":"2017","journal-title":"PLoS Pathog"},{"issue":"10","key":"ppat.1007956.ref012","doi-asserted-by":"crossref","first-page":"e1005927","DOI":"10.1371\/journal.ppat.1005927","article-title":"Spatial and Temporal Resolution of Global Protein Synthesis during HSV Infection Using Bioorthogonal Precursors and Click Chemistry","volume":"12","author":"C Su Hui Teo","year":"2016","journal-title":"PLoS Pathog"},{"issue":"8","key":"ppat.1007956.ref013","first-page":"1008","article-title":"Systems Analysis of Protein Fatty Acylation in Herpes Simplex Virus-Infected Cells Using Chemical Proteomics","volume":"22","author":"RA Serwa","year":"2015","journal-title":"Cell Chem Biol"},{"issue":"21","key":"ppat.1007956.ref014","doi-asserted-by":"crossref","first-page":"11107","DOI":"10.1128\/JVI.01950-15","article-title":"Remote Activation of Host Cell DNA Synthesis in Uninfected Cells Signaled by Infected Cells in Advance of Virus Transmission","volume":"89","author":"N Schmidt","year":"2015","journal-title":"J Virol"},{"key":"ppat.1007956.ref015","doi-asserted-by":"crossref","first-page":"21","DOI":"10.1016\/j.virusres.2018.08.003","article-title":"Recent trends in click chemistry as a promising technology for virus-related research","volume":"256","author":"T Ouyang","year":"2018","journal-title":"Virus Res"},{"issue":"13","key":"ppat.1007956.ref016","doi-asserted-by":"crossref","first-page":"1896","DOI":"10.1002\/1873-3468.12131","article-title":"Labeling of virus components for advanced, quantitative imaging analyses","volume":"590","author":"V Sakin","year":"2016","journal-title":"FEBS Lett"},{"issue":"17","key":"ppat.1007956.ref017","doi-asserted-by":"crossref","first-page":"4166","DOI":"10.1002\/anie.201108231","article-title":"Amino acids for Diels-Alder reactions in living cells","volume":"51","author":"T Plass","year":"2012","journal-title":"Angew Chem Int Ed Engl"},{"issue":"19","key":"ppat.1007956.ref018","doi-asserted-by":"crossref","first-page":"8364","DOI":"10.1021\/ac301918t","article-title":"A mild and reliable method to label enveloped virus with quantum dots by copper-free click chemistry","volume":"84","author":"J Hao","year":"2012","journal-title":"Anal Chem"},{"issue":"15","key":"ppat.1007956.ref019","doi-asserted-by":"crossref","first-page":"7546","DOI":"10.1128\/JVI.00118-11","article-title":"Unnatural amino acid incorporation onto adenoviral (Ad) coat proteins facilitates chemoselective modification and retargeting of Ad type 5 vectors","volume":"85","author":"PS Banerjee","year":"2011","journal-title":"J Virol"},{"issue":"5","key":"ppat.1007956.ref020","doi-asserted-by":"crossref","first-page":"1897","DOI":"10.1128\/JVI.63.5.1897-1906.1989","article-title":"Control of mRNA stability by the virion host shutoff function of herpes simplex virus","volume":"63","author":"AA Oroskar","year":"1989","journal-title":"J Virol"},{"issue":"3","key":"ppat.1007956.ref021","doi-asserted-by":"crossref","first-page":"1063","DOI":"10.1128\/JVI.78.3.1063-1068.2004","article-title":"Herpes simplex virus virion host shutoff protein: immune evasion mediated by a viral RNase?","volume":"78","author":"JR Smiley","year":"2004","journal-title":"J Virol"},{"key":"ppat.1007956.ref022","article-title":"Multifunctional reagents for quantitative proteome-wide analysis of protein modification in human cells and dynamic profiling of protein lipidation during vertebrate development","author":"M Broncel","year":"2015","journal-title":"Angewandte Chemie-International Edition"},{"issue":"8","key":"ppat.1007956.ref023","first-page":"20","article-title":"Identification of newly synthesized proteins using bioorthogonal noncanonical amino acid tagging (BONCAT)","volume":"6","author":"DC Dieterich","year":"2007","journal-title":"Molecular & Cellular Proteomics"},{"issue":"3","key":"ppat.1007956.ref024","doi-asserted-by":"crossref","first-page":"532","DOI":"10.1038\/nprot.2007.52","article-title":"Labeling, detection and identification of newly synthesized proteomes with bioorthogonal non-canonical amino-acid tagging","volume":"2","author":"DC Dieterich","year":"2007","journal-title":"Nature Protocols"},{"issue":"5","key":"ppat.1007956.ref025","doi-asserted-by":"crossref","first-page":"1352","DOI":"10.1074\/mcp.M113.031914","article-title":"Quantitative, time-resolved proteomic analysis by combining bioorthogonal noncanonical amino acid tagging and pulsed stable isotope labeling by amino acids in cell culture","volume":"13","author":"JD Bagert","year":"2014","journal-title":"Mol Cell Proteomics"},{"issue":"Pt 1","key":"ppat.1007956.ref026","doi-asserted-by":"crossref","first-page":"124","DOI":"10.1099\/vir.0.036145-0","article-title":"Fusion of a fluorescent protein to the pUL25 minor capsid protein of pseudorabies virus allows live-cell capsid imaging with negligible impact on infection","volume":"93","author":"KP Bohannon","year":"2012","journal-title":"J Gen Virol"},{"issue":"10","key":"ppat.1007956.ref027","doi-asserted-by":"crossref","DOI":"10.1128\/JVI.00320-17","article-title":"Quantitative Evaluation of Protein Heterogeneity within Herpes Simplex Virus 1 Particles.","volume":"91","author":"N El Bilali","year":"2017","journal-title":"J Virol"},{"issue":"Pt 2","key":"ppat.1007956.ref028","doi-asserted-by":"crossref","first-page":"269","DOI":"10.1099\/0022-1317-73-2-269","article-title":"Characterization of enveloped tegument structures (L particles) produced by alphaherpesviruses: integrity of the tegument does not depend on the presence of capsid or envelope","volume":"73","author":"J McLauchlan","year":"1992","journal-title":"J Gen Virol"},{"issue":"Pt 3","key":"ppat.1007956.ref029","doi-asserted-by":"crossref","first-page":"661","DOI":"10.1099\/0022-1317-72-3-661","article-title":"Identification and characterization of a novel non-infectious herpes simplex virus-related particle","volume":"72","author":"JF Szilagyi","year":"1991","journal-title":"J Gen Virol"},{"key":"ppat.1007956.ref030","volume-title":"Principles of Virology","author":"SJ Flint","year":"2009"},{"key":"ppat.1007956.ref031","doi-asserted-by":"crossref","first-page":"1007","DOI":"10.1083\/jcb.136.5.1007","article-title":"Microtubule-mediated transport of incoming herpes simplex virus 1 capsids to the nucleus","volume":"136","author":"B Sodeik","year":"1997","journal-title":"J Cell Biol"},{"key":"ppat.1007956.ref032","doi-asserted-by":"crossref","first-page":"627","DOI":"10.1146\/annurev.cellbio.16.1.627","article-title":"Viral entry into the nucleus","volume":"16","author":"GR Whittaker","year":"2000","journal-title":"Annu Rev Cell Dev Biol"},{"key":"ppat.1007956.ref033","doi-asserted-by":"crossref","first-page":"469538","DOI":"10.1155\/2013\/469538","article-title":"Virus entry by endocytosis","volume":"2013","author":"AV Nicola","year":"2013","journal-title":"Adv Virol"},{"issue":"7","key":"ppat.1007956.ref034","first-page":"e1007196 2018","article-title":"A Bimodal Switch in Global Protein Translation Coupled to eIF4H Relocalisation during Advancing Cell-Cell Transmission of Herpes Simplex Virus PLoS Pathog","volume":"14","author":"C Su Hui Teo","year":"2018"},{"issue":"3","key":"ppat.1007956.ref035","doi-asserted-by":"crossref","first-page":"135","DOI":"10.1006\/smvy.1993.1009","article-title":"Stucture and assembly of herpesviruses","volume":"4","author":"F Rixon","year":"1993","journal-title":"Seminars in Virology"},{"issue":"10","key":"ppat.1007956.ref036","doi-asserted-by":"crossref","first-page":"e25464","DOI":"10.1371\/journal.pone.0025464","article-title":"Entry pathways of herpes simplex virus type 1 into human keratinocytes are dynamin- and cholesterol-dependent","volume":"6","author":"E Rahn","year":"2011","journal-title":"PLoS One"},{"issue":"24","key":"ppat.1007956.ref037","doi-asserted-by":"crossref","first-page":"12844","DOI":"10.1128\/JVI.05853-11","article-title":"Microvesicles and viral infection","volume":"85","author":"DG Meckes","year":"2011","journal-title":"J Virol"},{"issue":"7","key":"ppat.1007956.ref038","doi-asserted-by":"crossref","first-page":"1370","DOI":"10.1021\/bc200477z","article-title":"Altering adenoviral tropism via click modification with ErbB specific ligands","volume":"23","author":"YH Oum","year":"2012","journal-title":"Bioconjug Chem"},{"key":"ppat.1007956.ref039","doi-asserted-by":"crossref","first-page":"95","DOI":"10.1016\/j.virol.2015.09.009","article-title":"Surface modification via strain-promoted click reaction facilitates targeted lentiviral transduction","volume":"487","author":"Y Chu","year":"2016","journal-title":"Virology"},{"issue":"1","key":"ppat.1007956.ref040","doi-asserted-by":"crossref","first-page":"105","DOI":"10.1038\/nprot.2011.425","article-title":"Multifunctional protein labeling via enzymatic N-terminal tagging and elaboration by click chemistry","volume":"7","author":"WP Heal","year":"2012","journal-title":"Nature Protocols"},{"key":"ppat.1007956.ref041","doi-asserted-by":"crossref","first-page":"453","DOI":"10.1007\/BF01025868","article-title":"On the Histogram as a Density Estimator: LZ Theory","volume":"57","author":"D Freedman","year":"1981","journal-title":"Zeitschrgt her Wahrsheinlichkeitstheorie und Verwandte Gebeite"},{"issue":"1","key":"ppat.1007956.ref042","doi-asserted-by":"crossref","first-page":"59","DOI":"10.1080\/00031305.1997.10473591","article-title":"Data-Based Choice of Histogram Bin Width","volume":"51","author":"MP Wand","year":"1997","journal-title":"The American Statistician"},{"issue":"12","key":"ppat.1007956.ref043","doi-asserted-by":"crossref","first-page":"1367","DOI":"10.1038\/nbt.1511","article-title":"MaxQuant enables high peptide identification rates, individualized p.p.b.-range mass accuracies and proteome-wide protein quantification","volume":"26","author":"J Cox","year":"2008","journal-title":"Nature Biotechnology"},{"issue":"4","key":"ppat.1007956.ref044","doi-asserted-by":"crossref","first-page":"M111","DOI":"10.1074\/mcp.M111.010587","article-title":"PEAKS DB: de novo sequencing assisted database search for sensitive and accurate peptide identification","volume":"11","author":"J Zhang","year":"2012","journal-title":"Mol Cell Proteomics"},{"key":"ppat.1007956.ref045","doi-asserted-by":"crossref","first-page":"D115","DOI":"10.1093\/nar\/gkh131","article-title":"UniProt: the Universal Protein knowledgebase","volume":"32","author":"R Apweiler","year":"2004","journal-title":"Nucleic Acids Res"}],"updated-by":[{"DOI":"10.1371\/journal.ppat.1007956","type":"new_version","label":"New version","source":"publisher","updated":{"date-parts":[[2019,10,17]],"date-time":"2019-10-17T00:00:00Z","timestamp":1571270400000}}],"container-title":["PLOS Pathogens"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/dx.plos.org\/10.1371\/journal.ppat.1007956","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,7,24]],"date-time":"2024-07-24T13:47:24Z","timestamp":1721828844000},"score":1,"resource":{"primary":{"URL":"https:\/\/dx.plos.org\/10.1371\/journal.ppat.1007956"}},"subtitle":[],"editor":[{"given":"Lindsey","family":"Hutt-Fletcher","sequence":"first","affiliation":[],"role":[{"role":"editor","vocabulary":"crossref"}]}],"short-title":[],"issued":{"date-parts":[[2019,10,7]]},"references-count":45,"journal-issue":{"issue":"10","published-online":{"date-parts":[[2019,10,7]]}},"URL":"https:\/\/doi.org\/10.1371\/journal.ppat.1007956","relation":{"has-preprint":[{"id-type":"doi","id":"10.1101\/693036","asserted-by":"object"}]},"ISSN":["1553-7374"],"issn-type":[{"value":"1553-7374","type":"electronic"}],"subject":[],"published":{"date-parts":[[2019,10,7]]}}}