{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,16]],"date-time":"2026-09-16T22:56:44Z","timestamp":1789599404757,"version":"build-2803163510"},"reference-count":50,"publisher":"Public Library of Science (PLoS)","issue":"5","license":[{"start":{"date-parts":[[2020,5,28]],"date-time":"2020-05-28T00:00:00Z","timestamp":1590624000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100004440","name":"Wellcome Trust","doi-asserted-by":"publisher","award":["083811\/Z\/07\/Z"],"award-info":[{"award-number":["083811\/Z\/07\/Z"]}],"id":[{"id":"10.13039\/100004440","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100004440","name":"Wellcome Trust","doi-asserted-by":"publisher","award":["107046\/Z\/15\/Z"],"award-info":[{"award-number":["107046\/Z\/15\/Z"]}],"id":[{"id":"10.13039\/100004440","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100004440","name":"Wellcome Trust","doi-asserted-by":"publisher","award":["WT104111AIA"],"award-info":[{"award-number":["WT104111AIA"]}],"id":[{"id":"10.13039\/100004440","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100011102","name":"Seventh Framework Programme","doi-asserted-by":"publisher","award":["242095"],"award-info":[{"award-number":["242095"]}],"id":[{"id":"10.13039\/100011102","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["www.plospathogens.org"],"crossmark-restriction":false},"short-container-title":["PLoS Pathog"],"DOI":"10.1371\/journal.ppat.1008091","type":"journal-article","created":{"date-parts":[[2020,5,28]],"date-time":"2020-05-28T13:22:55Z","timestamp":1590672175000},"page":"e1008091","update-policy":"https:\/\/doi.org\/10.1371\/journal.ppat.corrections_policy","source":"Crossref","is-referenced-by-count":5,"title":["Zygote morphogenesis but not the establishment of cell polarity in Plasmodium berghei is controlled by the small GTPase, RAB11A"],"prefix":"10.1371","volume":"16","author":[{"given":"Harshal","family":"Patil","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3597-1056","authenticated-orcid":true,"given":"Katie R.","family":"Hughes","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2832-6806","authenticated-orcid":true,"given":"Leandro","family":"Lemgruber","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nisha","family":"Philip","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0492-5855","authenticated-orcid":true,"given":"Nicholas","family":"Dickens","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"G. Lucas","family":"Starnes","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8900-2982","authenticated-orcid":true,"given":"Andrew. P.","family":"Waters","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"340","published-online":{"date-parts":[[2020,5,28]]},"reference":[{"issue":"6933","key":"ppat.1008091.ref001","doi-asserted-by":"crossref","first-page":"766","DOI":"10.1038\/nature01602","article-title":"Adaptation of core mechanisms to generate cell polarity","volume":"422","author":"WJ Nelson","year":"2003","journal-title":"Nature"},{"issue":"9","key":"ppat.1008091.ref002","doi-asserted-by":"crossref","first-page":"328","DOI":"10.1016\/0168-9525(94)90036-1","article-title":"Cell polarity in yeast","volume":"10","author":"J. Chant","year":"1994","journal-title":"Trends Genet"},{"key":"ppat.1008091.ref003","doi-asserted-by":"crossref","first-page":"599","DOI":"10.1146\/annurev-cellbio-092910-154244","article-title":"Cell polarity as a regulator of cancer cell behavior plasticity","volume":"28","author":"SK Muthuswamy","year":"2012","journal-title":"Annu Rev Cell Dev Biol"},{"issue":"12","key":"ppat.1008091.ref004","doi-asserted-by":"crossref","first-page":"483","DOI":"10.1016\/S0962-8924(01)02145-6","article-title":"Protein trafficking in the exocytic pathway of polarized epithelial cells","volume":"11","author":"WJ Nelson","year":"2001","journal-title":"Trends Cell Biol"},{"issue":"2","key":"ppat.1008091.ref005","doi-asserted-by":"crossref","first-page":"157","DOI":"10.1016\/S0955-0674(99)00071-X","article-title":"The Ras branch of small GTPases: Ras family members don't fall far from the tree","volume":"12","author":"GW Reuther","year":"2000","journal-title":"Curr Opin Cell Biol"},{"key":"ppat.1008091.ref006","article-title":"RAB GTPases and RAB-interacting proteins and their role in the control of cognitive functions","author":"P D'Adamo","year":"2014","journal-title":"Neurosci Biobehav Rev"},{"key":"ppat.1008091.ref007","doi-asserted-by":"crossref","first-page":"13","DOI":"10.1016\/S0378-1119(03)00381-0","article-title":"The Plasmodium falciparum family of Rab GTPases","volume":"306","author":"E Quevillon","year":"2003","journal-title":"Gene"},{"issue":"4","key":"ppat.1008091.ref008","doi-asserted-by":"crossref","first-page":"913","DOI":"10.1083\/jcb.135.4.913","article-title":"Rab11 regulates recycling through the pericentriolar recycling endosome","volume":"135","author":"O Ullrich","year":"1996","journal-title":"J Cell Biol"},{"issue":"1","key":"ppat.1008091.ref009","doi-asserted-by":"crossref","first-page":"e1000270","DOI":"10.1371\/journal.ppat.1000270","article-title":"Rab11A-controlled assembly of the inner membrane complex is required for completion of apicomplexan cytokinesis","volume":"5","author":"C Agop-Nersesian","year":"2009","journal-title":"PLoS Pathog"},{"issue":"6187","key":"ppat.1008091.ref010","doi-asserted-by":"crossref","first-page":"1035","DOI":"10.1126\/science.1253397","article-title":"Structures of PI4KIII\u03b2 complexes show simultaneous recruitment of Rab11 and its effectors","volume":"344","author":"JE Burke","year":"2014","journal-title":"Science"},{"issue":"7479","key":"ppat.1008091.ref011","doi-asserted-by":"crossref","first-page":"248","DOI":"10.1038\/nature12782","article-title":"Targeting Plasmodium PI(4)K to eliminate malaria","volume":"504","author":"CW McNamara","year":"2013","journal-title":"Nature"},{"issue":"6","key":"ppat.1008091.ref012","doi-asserted-by":"crossref","first-page":"1360","DOI":"10.1042\/BST20120157","article-title":"Rab11 proteins in health and disease","volume":"40","author":"EE Kelly","year":"2012","journal-title":"Biochem Soc Trans"},{"issue":"40","key":"ppat.1008091.ref013","doi-asserted-by":"crossref","first-page":"34245","DOI":"10.1074\/jbc.M504158200","article-title":"Proteomic analysis of rhoptry organelles reveals many novel constituents for host-parasite interactions in Toxoplasma gondii","volume":"280","author":"PJ Bradley","year":"2005","journal-title":"J Biol Chem"},{"issue":"12","key":"ppat.1008091.ref014","doi-asserted-by":"crossref","first-page":"1003","DOI":"10.1038\/nmeth1134","article-title":"Rapid control of protein level in the apicomplexan Toxoplasma gondii","volume":"4","author":"A Herm-Gotz","year":"2007","journal-title":"Nature methods"},{"issue":"5787","key":"ppat.1008091.ref015","doi-asserted-by":"crossref","first-page":"667","DOI":"10.1126\/science.1125129","article-title":"Regulation of sexual development of Plasmodium by translational repression","volume":"313","author":"GR Mair","year":"2006","journal-title":"Science"},{"issue":"11","key":"ppat.1008091.ref016","doi-asserted-by":"crossref","first-page":"493","DOI":"10.1186\/s13059-014-0493-0","article-title":"Genome-wide RIP-Chip analysis of translational repressor-bound mRNAs in the Plasmodium gametocyte","volume":"15","author":"A Guerreiro","year":"2014","journal-title":"Genome Biol"},{"issue":"1","key":"ppat.1008091.ref017","doi-asserted-by":"crossref","first-page":"86","DOI":"10.1186\/s12915-014-0086-0","article-title":"A comprehensive evaluation of rodent malaria parasite genomes and gene expression","volume":"12","author":"TD Otto","year":"2014","journal-title":"BMC Biol"},{"issue":"1","key":"ppat.1008091.ref018","doi-asserted-by":"crossref","first-page":"23","DOI":"10.1016\/j.molbiopara.2004.04.007","article-title":"A Plasmodium berghei reference line that constitutively expresses GFP at a high level throughout the complete life cycle","volume":"137","author":"B Franke-Fayard","year":"2004","journal-title":"Mol Biochem Parasitol"},{"issue":"12","key":"ppat.1008091.ref019","doi-asserted-by":"crossref","first-page":"1996","DOI":"10.1111\/j.1462-5822.2011.01686.x","article-title":"Stage-specific depletion of myosin A supports an essential role in motility of malarial ookinetes","volume":"13","author":"I Siden-Kiamos","year":"2011","journal-title":"Cell Microbiol"},{"issue":"12","key":"ppat.1008091.ref020","doi-asserted-by":"crossref","first-page":"1956","DOI":"10.1111\/j.1462-5822.2011.01683.x","article-title":"Experimentally controlled downregulation of the histone chaperone FACT in Plasmodium berghei reveals that it is critical to male gamete fertility","volume":"13","author":"EC Laurentino","year":"2011","journal-title":"Cell Microbiol"},{"issue":"1","key":"ppat.1008091.ref021","doi-asserted-by":"crossref","first-page":"60","DOI":"10.1016\/j.molbiopara.2005.09.007","article-title":"High efficiency transfection of Plasmodium berghei facilitates novel selection procedures","volume":"145","author":"CJ Janse","year":"2006","journal-title":"Mol Biochem Parasitol"},{"issue":"12","key":"ppat.1008091.ref022","doi-asserted-by":"crossref","first-page":"e1006094","DOI":"10.1371\/journal.ppat.1006094","article-title":"Stage-Specific Changes in Plasmodium Metabolism Required for Differentiation and Adaptation to Different Host and Vector Environments","volume":"12","author":"A Srivastava","year":"2016","journal-title":"PLoS Pathog"},{"issue":"7","key":"ppat.1008091.ref023","doi-asserted-by":"crossref","first-page":"905","DOI":"10.1111\/cmi.12604","article-title":"The development of malaria parasites in the mosquito midgut","volume":"18","author":"S Bennink","year":"2016","journal-title":"Cell Microbiol"},{"issue":"1","key":"ppat.1008091.ref024","doi-asserted-by":"crossref","DOI":"10.1101\/cshperspect.a027979","article-title":"Trafficking Ion Transporters to the Apical Membrane of Polarized Intestinal Enterocytes","volume":"10","author":"AC Engevik","year":"2018","journal-title":"Cold Spring Harb Perspect Biol"},{"issue":"2\u20133","key":"ppat.1008091.ref025","doi-asserted-by":"crossref","first-page":"197","DOI":"10.1016\/S0014-5793(01)02483-8","article-title":"Prenylation of Rab GTPases: molecular mechanisms and involvement in genetic disease","volume":"498","author":"JB Pereira-Leal","year":"2001","journal-title":"FEBS Lett"},{"issue":"44","key":"ppat.1008091.ref026","doi-asserted-by":"crossref","first-page":"42066","DOI":"10.1074\/jbc.M202860200","article-title":"Protein farnesyltransferase and protein prenylation in Plasmodium falciparum","volume":"277","author":"D Chakrabarti","year":"2002","journal-title":"J Biol Chem"},{"issue":"1","key":"ppat.1008091.ref027","doi-asserted-by":"crossref","first-page":"9","DOI":"10.1016\/j.chom.2012.05.014","article-title":"A Plasmodium calcium-dependent protein kinase controls zygote development and transmission by translationally activating repressed mRNAs","volume":"12","author":"S Sebastian","year":"2012","journal-title":"Cell Host Microbe"},{"issue":"9","key":"ppat.1008091.ref028","doi-asserted-by":"crossref","first-page":"e1000599","DOI":"10.1371\/journal.ppat.1000599","article-title":"A cyclic GMP signalling module that regulates gliding motility in a malaria parasite","volume":"5","author":"RW Moon","year":"2009","journal-title":"PLoS Pathog"},{"issue":"15","key":"ppat.1008091.ref029","doi-asserted-by":"crossref","first-page":"e100984","DOI":"10.15252\/embj.2018100984","article-title":"Microtubule number and length determine cellular shape and function in Plasmodium","volume":"38","author":"B Spreng","year":"2019","journal-title":"Embo j"},{"issue":"1","key":"ppat.1008091.ref030","doi-asserted-by":"crossref","first-page":"27","DOI":"10.1016\/0035-9203(63)90007-5","article-title":"Electron microscope studies of motile stages of malaria parasites. IV. The fine structure of the sporozoites of four species of plasmodium","volume":"57","author":"PCC Garnham","year":"1963","journal-title":"Transactions of The Royal Society of Tropical Medicine and Hygiene"},{"issue":"1","key":"ppat.1008091.ref031","doi-asserted-by":"crossref","first-page":"21","DOI":"10.1128\/MMBR.66.1.21-38.2002","article-title":"Cytoskeleton of apicomplexan parasites","volume":"66","author":"NS Morrissette","year":"2002","journal-title":"Microbiol Mol Biol Rev"},{"issue":"Pt 5","key":"ppat.1008091.ref032","doi-asserted-by":"crossref","first-page":"425","DOI":"10.1017\/S003118209800328X","article-title":"Microtubules in Plasmodium falciparum merozoites and their importance for invasion of erythrocytes","volume":"117","author":"RE Fowler","year":"1998","journal-title":"Parasitology"},{"issue":"11","key":"ppat.1008091.ref033","doi-asserted-by":"crossref","first-page":"1160","DOI":"10.1242\/bio.20136163","article-title":"Unique apicomplexan IMC sub-compartment proteins are early markers for apical polarity in the malaria parasite","volume":"2","author":"B Poulin","year":"2013","journal-title":"Biol Open"},{"issue":"46","key":"ppat.1008091.ref034","doi-asserted-by":"crossref","first-page":"31871","DOI":"10.1074\/jbc.M800028200","article-title":"Centrins, cell cycle regulation proteins in human malaria parasite Plasmodium falciparum","volume":"283","author":"B Mahajan","year":"2008","journal-title":"J Biol Chem"},{"issue":"9","key":"ppat.1008091.ref035","doi-asserted-by":"crossref","first-page":"586","DOI":"10.1002\/cm.20469","article-title":"RNG1 is a late marker of the apical polar ring in Toxoplasma gondii","volume":"67","author":"JQ Tran","year":"2010","journal-title":"Cytoskeleton (Hoboken)"},{"key":"ppat.1008091.ref036","article-title":"Cell division in apicomplexan parasites","author":"ME Francia","year":"2014","journal-title":"Nat Rev Microbiol"},{"key":"ppat.1008091.ref037","doi-asserted-by":"crossref","first-page":"103","DOI":"10.1186\/1475-2875-11-103","article-title":"Improved negative selection protocol for Plasmodium berghei in the rodent malarial model","volume":"11","author":"RY Orr","year":"2012","journal-title":"Malar J"},{"issue":"4","key":"ppat.1008091.ref038","doi-asserted-by":"crossref","first-page":"e1000853","DOI":"10.1371\/journal.ppat.1000853","article-title":"Three members of the 6-cys protein family of Plasmodium play a role in gamete fertility","volume":"6","author":"MR van Dijk","year":"2010","journal-title":"PLoS Pathog"},{"issue":"1","key":"ppat.1008091.ref039","doi-asserted-by":"crossref","first-page":"153","DOI":"10.1016\/S0092-8674(01)00199-4","article-title":"A central role for P48\/45 in malaria parasite male gamete fertility","volume":"104","author":"MR van Dijk","year":"2001","journal-title":"Cell"},{"key":"ppat.1008091.ref040","doi-asserted-by":"crossref","first-page":"183","DOI":"10.1016\/B978-0-12-391857-4.00009-4","article-title":"Methods for cell and particle tracking","volume":"504","author":"E Meijering","year":"2012","journal-title":"Methods Enzymol"},{"issue":"9","key":"ppat.1008091.ref041","doi-asserted-by":"crossref","first-page":"e44617","DOI":"10.1371\/journal.pone.0044617","article-title":"A unique Kelch domain phosphatase in Plasmodium regulates ookinete morphology, motility and invasion","volume":"7","author":"N Philip","year":"2012","journal-title":"PLoS One"},{"issue":"9","key":"ppat.1008091.ref042","doi-asserted-by":"crossref","first-page":"e108930","DOI":"10.1371\/journal.pone.0108930","article-title":"P. berghei telomerase subunit TERT is essential for parasite survival","volume":"9","author":"AA Religa","year":"2014","journal-title":"PLoS One"},{"issue":"2","key":"ppat.1008091.ref043","doi-asserted-by":"crossref","first-page":"e1000767","DOI":"10.1371\/journal.ppat.1000767","article-title":"Universal features of post-transcriptional gene regulation are critical for Plasmodium zygote development","volume":"6","author":"GR Mair","year":"2010","journal-title":"PLoS Pathog"},{"key":"ppat.1008091.ref044","article-title":"Cutadapt removes adapter sequences from high-throughput sequencing reads","volume":"17","author":"M. Martin","year":"2011","journal-title":"EMBLnet Journal Bioinformatics in action"},{"key":"ppat.1008091.ref045","doi-asserted-by":"crossref","first-page":"D539","DOI":"10.1093\/nar\/gkn814","article-title":"PlasmoDB: a functional genomic database for malaria parasites","volume":"37","author":"C Aurrecoechea","year":"2009","journal-title":"Nucleic Acids Res"},{"issue":"4","key":"ppat.1008091.ref046","doi-asserted-by":"crossref","first-page":"R36","DOI":"10.1186\/gb-2013-14-4-r36","article-title":"TopHat2: accurate alignment of transcriptomes in the presence of insertions, deletions and gene fusions","volume":"14","author":"D Kim","year":"2013","journal-title":"Genome Biol"},{"issue":"4","key":"ppat.1008091.ref047","doi-asserted-by":"crossref","first-page":"357","DOI":"10.1038\/nmeth.1923","article-title":"Fast gapped-read alignment with Bowtie2","volume":"9","author":"B Langmead","year":"2012","journal-title":"Nat Methods"},{"issue":"1","key":"ppat.1008091.ref048","doi-asserted-by":"crossref","first-page":"46","DOI":"10.1038\/nbt.2450","article-title":"Differential analysis of gene regulation at transcript resolution with RNA-seq","volume":"31","author":"C Trapnell","year":"2013","journal-title":"Nat Biotechnol"},{"key":"ppat.1008091.ref049","doi-asserted-by":"crossref","first-page":"86","DOI":"10.1186\/s12915-014-0086-0","article-title":"A comprehensive evaluation of rodent malaria parasite genomes and gene expression","volume":"12","author":"TD Otto","year":"2014","journal-title":"BMC Biol"},{"issue":"1","key":"ppat.1008091.ref050","doi-asserted-by":"crossref","first-page":"734","DOI":"10.1186\/s12864-017-4100-0","article-title":"Comparative transcriptomics of female and male gametocytes in Plasmodium berghei and the evolution of sex in alveolates","volume":"18","author":"LM Yeoh","year":"2017","journal-title":"BMC Genomics"}],"container-title":["PLOS Pathogens"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/dx.plos.org\/10.1371\/journal.ppat.1008091","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2020,5,28]],"date-time":"2020-05-28T13:23:33Z","timestamp":1590672213000},"score":1,"resource":{"primary":{"URL":"https:\/\/dx.plos.org\/10.1371\/journal.ppat.1008091"}},"subtitle":[],"editor":[{"given":"Michael J.","family":"Blackman","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2020,5,28]]},"references-count":50,"journal-issue":{"issue":"5","published-online":{"date-parts":[[2020,5,28]]}},"URL":"https:\/\/doi.org\/10.1371\/journal.ppat.1008091","relation":{"has-preprint":[{"id-type":"doi","id":"10.1101\/811802","asserted-by":"object"}]},"ISSN":["1553-7374"],"issn-type":[{"value":"1553-7374","type":"electronic"}],"subject":[],"published":{"date-parts":[[2020,5,28]]}}}