{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,22]],"date-time":"2026-04-22T16:55:30Z","timestamp":1776876930774,"version":"3.51.2"},"update-to":[{"DOI":"10.1371\/journal.ppat.1008299","type":"new_version","label":"New version","source":"publisher","updated":{"date-parts":[[2021,2,10]],"date-time":"2021-02-10T00:00:00Z","timestamp":1612915200000}}],"reference-count":46,"publisher":"Public Library of Science (PLoS)","issue":"1","license":[{"start":{"date-parts":[[2021,1,19]],"date-time":"2021-01-19T00:00:00Z","timestamp":1611014400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["R01AI136538"],"award-info":[{"award-number":["R01AI136538"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["R01AI121090"],"award-info":[{"award-number":["R01AI121090"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000861","name":"Burroughs Wellcome Fund","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100000861","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["F32AI138414"],"award-info":[{"award-number":["F32AI138414"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["www.plospathogens.org"],"crossmark-restriction":false},"short-container-title":["PLoS Pathog"],"abstract":"<jats:p>\n                    Host resistance against intracellular pathogens requires a rapid IFN-\u03b3 mediated immune response. We reveal that T-bet-dependent production of IFN-\u03b3 is essential for the maintenance of inflammatory DCs at the site of infection with a common protozoan parasite,\n                    <jats:italic>Toxoplasma gondii<\/jats:italic>\n                    . A detailed analysis of the cellular sources for T-bet-dependent IFN-\u03b3 identified that ILC1s and to a lesser degree NK, but not T\n                    <jats:sub>H<\/jats:sub>\n                    1 cells, were involved in the regulation of inflammatory DCs via IFN-\u03b3. Mechanistically, we established that T-bet dependent innate IFN-\u03b3 is critical for the induction of IRF8, an essential transcription factor for cDC1s. Failure to upregulate IRF8 in DCs resulted in acute susceptibility to\n                    <jats:italic>T<\/jats:italic>\n                    .\n                    <jats:italic>gondii<\/jats:italic>\n                    infection. Our data identifies that T-bet dependent production of IFN-\u03b3 by ILC1 and NK cells is indispensable for host resistance against intracellular infection via maintaining IRF8+ inflammatory DCs at the site of infection.\n                  <\/jats:p>","DOI":"10.1371\/journal.ppat.1008299","type":"journal-article","created":{"date-parts":[[2021,1,19]],"date-time":"2021-01-19T13:45:29Z","timestamp":1611063929000},"page":"e1008299","update-policy":"https:\/\/doi.org\/10.1371\/journal.ppat.corrections_policy","source":"Crossref","is-referenced-by-count":47,"title":["T-bet-dependent ILC1- and NK cell-derived IFN-\u03b3 mediates cDC1-dependent host resistance against Toxoplasma gondii"],"prefix":"10.1371","volume":"17","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-6797-2179","authenticated-orcid":true,"given":"Am\u00e9rico H.","family":"L\u00f3pez-Yglesias","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4309-7725","authenticated-orcid":true,"given":"Elise","family":"Burger","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0151-077X","authenticated-orcid":true,"given":"Ellie","family":"Camanzo","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5790-3054","authenticated-orcid":true,"given":"Andrew T.","family":"Martin","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7568-074X","authenticated-orcid":true,"given":"Alessandra M.","family":"Araujo","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Samantha F.","family":"Kwok","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5825-8002","authenticated-orcid":true,"given":"Felix","family":"Yarovinsky","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"340","published-online":{"date-parts":[[2021,1,19]]},"reference":[{"issue":"7","key":"ppat.1008299.ref001","doi-asserted-by":"crossref","first-page":"4593","DOI":"10.4049\/jimmunol.175.7.4593","article-title":"Increased susceptibility of mice lacking T-bet to infection with Mycobacterium tuberculosis correlates with increased IL-10 and decreased IFN-gamma production","volume":"175","author":"BM Sullivan","year":"2005","journal-title":"Journal of immunology"},{"issue":"5553","key":"ppat.1008299.ref002","doi-asserted-by":"crossref","first-page":"338","DOI":"10.1126\/science.1065543","article-title":"Distinct effects of T-bet in TH1 lineage commitment and IFN-gamma production in CD4 and CD8 T cells","volume":"295","author":"SJ Szabo","year":"2002","journal-title":"Science"},{"issue":"7","key":"ppat.1008299.ref003","doi-asserted-by":"crossref","first-page":"4603","DOI":"10.4049\/jimmunol.175.7.4603","article-title":"Expression of T-bet by CD4 T cells is essential for resistance to Salmonella infection","volume":"175","author":"R Ravindran","year":"2005","journal-title":"Journal of immunology"},{"issue":"3","key":"ppat.1008299.ref004","doi-asserted-by":"crossref","first-page":"1131","DOI":"10.4049\/jimmunol.1401617","article-title":"Diverse roles for T-bet in the effector responses required for resistance to infection","volume":"194","author":"G Harms Pritchard","year":"2015","journal-title":"Journal of immunology"},{"key":"ppat.1008299.ref005","article-title":"T-bet-independent Th1 response induces intestinal immunopathology during Toxoplasma gondii infection","author":"AH L\u00f3pez-Yglesias","year":"2018","journal-title":"Mucosal immunology"},{"key":"ppat.1008299.ref006","article-title":"The evolving role of T-bet in resistance to infection","author":"GH Pritchard","year":"2019","journal-title":"Nat Rev Immunol"},{"issue":"6","key":"ppat.1008299.ref007","doi-asserted-by":"crossref","first-page":"655","DOI":"10.1016\/S0092-8674(00)80702-3","article-title":"A novel transcription factor, T-bet, directs Th1 lineage commitment","volume":"100","author":"SJ Szabo","year":"2000","journal-title":"Cell"},{"issue":"6","key":"ppat.1008299.ref008","doi-asserted-by":"crossref","first-page":"983","DOI":"10.1016\/j.immuni.2017.05.005","article-title":"The Transcription Factor T-bet Limits Amplification of Type I IFN Transcriptome and Circuitry in T Helper 1","volume":"46","author":"S Iwata","year":"2017","journal-title":"Cells. 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