{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,15]],"date-time":"2026-10-15T04:52:10Z","timestamp":1792039930668,"version":"4.4.0"},"update-to":[{"DOI":"10.1371\/journal.ppat.1008317","type":"new_version","label":"New version","source":"publisher","updated":{"date-parts":[[2020,3,5]],"date-time":"2020-03-05T00:00:00Z","timestamp":1583366400000}}],"reference-count":87,"publisher":"Public Library of Science (PLoS)","issue":"2","license":[{"start":{"date-parts":[[2020,2,24]],"date-time":"2020-02-24T00:00:00Z","timestamp":1582502400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001711","name":"Schweizerischer Nationalfonds zur F\u00f6rderung der Wissenschaftlichen Forschung","doi-asserted-by":"publisher","award":["140803"],"award-info":[{"award-number":["140803"]}],"id":[{"id":"10.13039\/501100001711","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001711","name":"Schweizerischer Nationalfonds zur F\u00f6rderung der Wissenschaftlichen Forschung","doi-asserted-by":"publisher","award":["125389"],"award-info":[{"award-number":["125389"]}],"id":[{"id":"10.13039\/501100001711","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001711","name":"Schweizerischer Nationalfonds zur F\u00f6rderung der Wissenschaftlichen Forschung","doi-asserted-by":"publisher","award":["PR00P3_179813"],"award-info":[{"award-number":["PR00P3_179813"]}],"id":[{"id":"10.13039\/501100001711","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["www.plospathogens.org"],"crossmark-restriction":false},"short-container-title":["PLoS Pathog"],"DOI":"10.1371\/journal.ppat.1008317","type":"journal-article","created":{"date-parts":[[2020,2,24]],"date-time":"2020-02-24T13:45:38Z","timestamp":1582551938000},"page":"e1008317","update-policy":"https:\/\/doi.org\/10.1371\/journal.ppat.corrections_policy","source":"Crossref","is-referenced-by-count":21,"title":["Phosphoinositide-binding proteins mark, shape and functionally modulate highly-diverged endocytic compartments in the parasitic protist Giardia lamblia"],"prefix":"10.1371","volume":"16","author":[{"given":"Lenka","family":"Cernikova","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1831-9365","authenticated-orcid":true,"given":"Carmen","family":"Faso","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2110-4445","authenticated-orcid":true,"given":"Adrian B.","family":"Hehl","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"340","published-online":{"date-parts":[[2020,2,24]]},"reference":[{"key":"ppat.1008317.ref001","doi-asserted-by":"crossref","first-page":"1019","DOI":"10.1152\/physrev.00028.2012","article-title":"Phosphoinositides: tiny lipids with giant impact on cell regulation","volume":"93","author":"T. Balla","year":"2013","journal-title":"Physiol Rev"},{"key":"ppat.1008317.ref002","doi-asserted-by":"crossref","first-page":"651","DOI":"10.1038\/nature05185","article-title":"Phosphoinositides in cell regulation and membrane dynamics","volume":"443","author":"G. Di Paolo","year":"2006","journal-title":"Nature"},{"key":"ppat.1008317.ref003","doi-asserted-by":"crossref","first-page":"10877","DOI":"10.1074\/jbc.R400038200","article-title":"Metabolic insights into phospholipid function using gene-targeted mice","volume":"280","author":"J.E. Vance","year":"2005","journal-title":"J Biol Chem"},{"key":"ppat.1008317.ref004","doi-asserted-by":"crossref","first-page":"429","DOI":"10.1016\/j.ceb.2006.06.006","article-title":"Lipid metabolism and dynamics during phagocytosis","volume":"18","author":"T. Yeung","year":"2006","journal-title":"Curr Opin Cell Biol"},{"key":"ppat.1008317.ref005","doi-asserted-by":"crossref","first-page":"517","DOI":"10.1038\/nrm3151","article-title":"Molecular mechanism and physiological functions of clathrin-mediated endocytosis","volume":"12","author":"H.T. McMahon","year":"2011","journal-title":"Nat Rev Mol Cell Biol"},{"key":"ppat.1008317.ref006","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1111\/j.1600-0854.2011.01246.x","article-title":"Phosphatidylinositol-3,5-bisphosphate: no longer the poor PIP2","volume":"13","author":"C.Y. Ho","year":"2012","journal-title":"Traffic"},{"key":"ppat.1008317.ref007","doi-asserted-by":"crossref","first-page":"900","DOI":"10.1002\/bies.201300064","article-title":"Phosphatidylinositol 3-phosphate, a lipid that regulates membrane dynamics, protein sorting and cell signalling","volume":"35","author":"K.O. Schink","year":"2013","journal-title":"Bioessays"},{"key":"ppat.1008317.ref008","doi-asserted-by":"crossref","first-page":"4239","DOI":"10.1242\/jcs.01287","article-title":"Alfy, a novel FYVE-domain-containing protein associated with protein granules and autophagic membranes","volume":"117","author":"A. Simonsen","year":"2004","journal-title":"J Cell Sci"},{"key":"ppat.1008317.ref009","doi-asserted-by":"crossref","first-page":"260","DOI":"10.1002\/bies.201300132","article-title":"Phosphatidylinositol 5-phosphate: a nuclear stress lipid and a tuner of membranes and cytoskeleton dynamics","volume":"36","author":"J. Viaud","year":"2014","journal-title":"Bioessays"},{"key":"ppat.1008317.ref010","doi-asserted-by":"crossref","first-page":"507","DOI":"10.1038\/nchembio.390","article-title":"Translation of the phosphoinositide code by PI effectors","volume":"6","author":"T.G. Kutateladze","year":"2010","journal-title":"Nat Chem Biol"},{"key":"ppat.1008317.ref011","doi-asserted-by":"crossref","first-page":"1399","DOI":"10.1016\/S0960-9822(98)00022-0","article-title":"Phosphatidylinositol-4,5-bisphosphate is required for endocytic coated vesicle formation","volume":"8","author":"M. Jost","year":"1998","journal-title":"Curr Biol"},{"key":"ppat.1008317.ref012","doi-asserted-by":"crossref","first-page":"523","DOI":"10.1016\/S0092-8674(02)00735-3","article-title":"Molecular architecture and functional model of the endocytic AP2 complex","volume":"109","author":"B.M. Collins","year":"2002","journal-title":"Cell"},{"key":"ppat.1008317.ref013","doi-asserted-by":"crossref","first-page":"755","DOI":"10.1083\/jcb.146.4.755","article-title":"Phosphoinositide-AP-2 interactions required for targeting to plasma membrane clathrin-coated pits","volume":"146","author":"I. Gaidarov","year":"1999","journal-title":"J Cell Biol"},{"key":"ppat.1008317.ref014","doi-asserted-by":"crossref","first-page":"1220","DOI":"10.1016\/j.cell.2010.05.006","article-title":"A large-scale conformational change couples membrane recruitment to cargo binding in the AP2 clathrin adaptor complex","volume":"141","author":"L.P. Jackson","year":"2010","journal-title":"Cell"},{"key":"ppat.1008317.ref015","doi-asserted-by":"crossref","first-page":"209","DOI":"10.1083\/jcb.200203103","article-title":"A phosphatidylinositol (4,5)-bisphosphate binding site within mu2-adaptin regulates clathrin-mediated endocytosis","volume":"158","author":"G. Rohde","year":"2002","journal-title":"J Cell Biol"},{"key":"ppat.1008317.ref016","doi-asserted-by":"crossref","first-page":"1051","DOI":"10.1126\/science.291.5506.1051","article-title":"Simultaneous binding of PtdIns(4,5)P2 and clathrin by AP180 in the nucleation of clathrin lattices on membranes","volume":"291","author":"M.G. Ford","year":"2001","journal-title":"Science"},{"key":"ppat.1008317.ref017","doi-asserted-by":"crossref","first-page":"1047","DOI":"10.1126\/science.291.5506.1047","article-title":"Role of the ENTH domain in phosphatidylinositol-4,5-bisphosphate binding and endocytosis","volume":"291","author":"T. Itoh","year":"2001","journal-title":"Science"},{"key":"ppat.1008317.ref018","doi-asserted-by":"crossref","first-page":"361","DOI":"10.1038\/nature01020","article-title":"Curvature of clathrin-coated pits driven by epsin","volume":"419","author":"M.G. Ford","year":"2002","journal-title":"Nature"},{"key":"ppat.1008317.ref019","doi-asserted-by":"crossref","first-page":"e1005756","DOI":"10.1371\/journal.ppat.1005756","article-title":"Static Clathrin Assemblies at the Peripheral Vacuole-Plasma Membrane Interface of the Parasitic Protozoan Giardia lamblia","volume":"12","author":"J.P. Zumthor","year":"2016","journal-title":"PLoS Pathog"},{"key":"ppat.1008317.ref020","doi-asserted-by":"crossref","first-page":"606","DOI":"10.1016\/j.parint.2017.04.008","article-title":"Phosphoinositide binding profiles of the PX domains of Giardia lamblia","volume":"66","author":"A. Jana","year":"2017","journal-title":"Parasitology international"},{"key":"ppat.1008317.ref021","doi-asserted-by":"crossref","first-page":"24","DOI":"10.1016\/j.molbiopara.2014.09.008","article-title":"The single epsin homolog in Giardia lamblia localizes to the ventral disk of trophozoites and is not associated with clathrin membrane coats","volume":"197","author":"J.A. Ebneter","year":"2014","journal-title":"Mol Biochem Parasitol"},{"key":"ppat.1008317.ref022","doi-asserted-by":"crossref","first-page":"1179","DOI":"10.1007\/s00284-010-9845-5","article-title":"Identification and characterization of a FYVE domain from the early diverging eukaryote Giardia lamblia","volume":"62","author":"A. Sinha","year":"2011","journal-title":"Curr Microbiol"},{"key":"ppat.1008317.ref023","article-title":"Membrane-Associated Proteins in Giardia lamblia","volume":"9","author":"M.C. Touz","year":"2018","journal-title":"Genes (Basel)"},{"key":"ppat.1008317.ref024","doi-asserted-by":"crossref","first-page":"1089","DOI":"10.1038\/sj.embor.7400004","article-title":"Identification of a family of endocytic proteins that define a new alpha-adaptin ear-binding motif","volume":"4","author":"B. Ritter","year":"2003","journal-title":"EMBO Rep"},{"key":"ppat.1008317.ref025","doi-asserted-by":"crossref","first-page":"2237","DOI":"10.1016\/j.jmb.2017.12.007","article-title":"A Completely Reimplemented MPI Bioinformatics Toolkit with a New HHpred Server at its Core","volume":"430","author":"L. Zimmermann","year":"2018","journal-title":"J Mol Biol"},{"key":"ppat.1008317.ref026","doi-asserted-by":"crossref","first-page":"D257","DOI":"10.1093\/nar\/gku949","article-title":"SMART: recent updates, new developments and status in 2015","volume":"43","author":"I. Letunic","year":"2015","journal-title":"Nucleic Acids Res"},{"key":"ppat.1008317.ref027","doi-asserted-by":"crossref","first-page":"D493","DOI":"10.1093\/nar\/gkx922","article-title":"20 years of the SMART protein domain annotation resource","volume":"46","author":"I. Letunic","year":"2018","journal-title":"Nucleic Acids Res"},{"key":"ppat.1008317.ref028","doi-asserted-by":"crossref","first-page":"1236","DOI":"10.1093\/bioinformatics\/btu031","article-title":"InterProScan 5: genome-scale protein function classification","volume":"30","author":"P. Jones","year":"2014","journal-title":"Bioinformatics"},{"key":"ppat.1008317.ref029","doi-asserted-by":"crossref","first-page":"434","DOI":"10.1038\/46594","article-title":"Crystal structures of the membrane-binding C2 domain of human coagulation factor V","volume":"402","author":"S. Macedo-Ribeiro","year":"1999","journal-title":"Nature"},{"key":"ppat.1008317.ref030","doi-asserted-by":"crossref","first-page":"45","DOI":"10.1186\/1471-2180-6-45","article-title":"Evidence from bioinformatics, expression and inhibition studies of phosphoinositide-3 kinase signalling in Giardia intestinalis","volume":"6","author":"S.S. Cox","year":"2006","journal-title":"BMC Microbiol"},{"key":"ppat.1008317.ref031","doi-asserted-by":"crossref","first-page":"29","DOI":"10.1111\/j.1550-7408.2006.00142.x","article-title":"Transcriptional analysis of three major putative phosphatidylinositol kinase genes in a parasitic protozoan, Giardia lamblia","volume":"54","author":"Y. Hernandez","year":"2007","journal-title":"J Eukaryot Microbiol"},{"key":"ppat.1008317.ref032","doi-asserted-by":"crossref","first-page":"40","DOI":"10.1186\/1471-2105-9-40","article-title":"I-TASSER server for protein 3D structure prediction","volume":"9","author":"Y. Zhang","year":"2008","journal-title":"BMC Bioinformatics"},{"key":"ppat.1008317.ref033","doi-asserted-by":"crossref","first-page":"725","DOI":"10.1038\/nprot.2010.5","article-title":"I-TASSER: a unified platform for automated protein structure and function prediction","volume":"5","author":"A. Roy","year":"2010","journal-title":"Nat Protoc"},{"key":"ppat.1008317.ref034","doi-asserted-by":"crossref","first-page":"7","DOI":"10.1038\/nmeth.3213","article-title":"The I-TASSER Suite: protein structure and function prediction","volume":"12","author":"J. Yang","year":"2015","journal-title":"Nat Methods"},{"key":"ppat.1008317.ref035","doi-asserted-by":"crossref","first-page":"44179","DOI":"10.1074\/jbc.M108811200","article-title":"All phox homology (PX) domains from Saccharomyces cerevisiae specifically recognize phosphatidylinositol 3-phosphate","volume":"276","author":"J.W. Yu","year":"2001","journal-title":"J Biol Chem"},{"key":"ppat.1008317.ref036","doi-asserted-by":"crossref","first-page":"3991","DOI":"10.1242\/jcs.114.22.3991","article-title":"FENS-1 and DFCP1 are FYVE domain-containing proteins with distinct functions in the endosomal and Golgi compartments","volume":"114","author":"S.H. Ridley","year":"2001","journal-title":"J Cell Sci"},{"key":"ppat.1008317.ref037","doi-asserted-by":"crossref","first-page":"26379","DOI":"10.1074\/jbc.M201106200","article-title":"Phosphatidylinositol 3-phosphate induces the membrane penetration of the FYVE domains of Vps27p and Hrs","volume":"277","author":"R.V. Stahelin","year":"2002","journal-title":"J Biol Chem"},{"key":"ppat.1008317.ref038","doi-asserted-by":"crossref","first-page":"53818","DOI":"10.1074\/jbc.M408408200","article-title":"The molecular basis of the differential subcellular localization of FYVE domains","volume":"279","author":"N.R. Blatner","year":"2004","journal-title":"J Biol Chem"},{"issue":"Pt 1","key":"ppat.1008317.ref039","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1042\/bj3500001","article-title":"Signal-dependent membrane targeting by pleckstrin homology (PH) domains","volume":"350","author":"M.A. Lemmon","year":"2000","journal-title":"Biochem J"},{"key":"ppat.1008317.ref040","doi-asserted-by":"crossref","first-page":"707","DOI":"10.1042\/BST0320707","article-title":"Pleckstrin homology domains: not just for phosphoinositides","volume":"32","author":"M.A. Lemmon","year":"2004","journal-title":"Biochem Soc Trans"},{"key":"ppat.1008317.ref041","doi-asserted-by":"crossref","first-page":"850","DOI":"10.1016\/j.bbalip.2006.04.008","article-title":"Membrane and juxtamembrane targeting by PH and PTB domains","volume":"1761","author":"J.P. DiNitto","year":"2006","journal-title":"Biochim Biophys Acta"},{"key":"ppat.1008317.ref042","doi-asserted-by":"crossref","first-page":"e00839","DOI":"10.1128\/mBio.00839-13","article-title":"Live-cell imaging of phosphoinositide dynamics and membrane architecture during Legionella infection","volume":"5","author":"S. Weber","year":"2014","journal-title":"MBio"},{"key":"ppat.1008317.ref043","doi-asserted-by":"crossref","first-page":"213","DOI":"10.1083\/jcb.150.1.213","article-title":"Intracellular pH regulation by Na(+)\/H(+) exchange requires phosphatidylinositol 4,5-bisphosphate","volume":"150","author":"O. Aharonovitz","year":"2000","journal-title":"J Cell Biol"},{"key":"ppat.1008317.ref044","doi-asserted-by":"crossref","first-page":"1063","DOI":"10.1016\/S0022-2275(20)40692-3","article-title":"Purification of polyphosphoinositides by chromatography on immobilized neomycin","volume":"19","author":"J. Schacht","year":"1978","journal-title":"J Lipid Res"},{"key":"ppat.1008317.ref045","doi-asserted-by":"crossref","first-page":"e1006036","DOI":"10.1371\/journal.ppat.1006036","article-title":"An Interactome-Centered Protein Discovery Approach Reveals Novel Components Involved in Mitosome Function and Homeostasis in Giardia lamblia","volume":"12","author":"S. Rout","year":"2016","journal-title":"PLoS Pathog"},{"key":"ppat.1008317.ref046","doi-asserted-by":"crossref","first-page":"130","DOI":"10.1016\/S0968-0004(99)01362-6","article-title":"START: a lipid-binding domain in StAR, HD-ZIP and signalling proteins","volume":"24","author":"C.P. Ponting","year":"1999","journal-title":"Trends Biochem Sci"},{"key":"ppat.1008317.ref047","doi-asserted-by":"crossref","first-page":"35996","DOI":"10.1074\/jbc.M806545200","article-title":"Characterization of SNAREs determines the absence of a typical Golgi apparatus in the ancient eukaryote Giardia lamblia","volume":"283","author":"E.V. Elias","year":"2008","journal-title":"J Biol Chem"},{"key":"ppat.1008317.ref048","doi-asserted-by":"crossref","first-page":"R66","DOI":"10.1186\/gb-2011-12-7-r66","article-title":"The minimal kinome of Giardia lamblia illuminates early kinase evolution and unique parasite biology","volume":"12","author":"G. Manning","year":"2011","journal-title":"Genome Biol"},{"key":"ppat.1008317.ref049","doi-asserted-by":"crossref","first-page":"539","DOI":"10.1186\/s13071-018-3112-1","article-title":"Multiple paralogues of alpha-SNAP in Giardia lamblia exhibit independent subcellular localization and redistribution during encystation and stress","volume":"11","author":"S.P. Datta","year":"2018","journal-title":"Parasit Vectors"},{"key":"ppat.1008317.ref050","doi-asserted-by":"crossref","first-page":"41","DOI":"10.1006\/jmbi.2001.5378","article-title":"Classification and evolution of P-loop GTPases and related ATPases","volume":"317","author":"D.D. Leipe","year":"2002","journal-title":"J Mol Biol"},{"key":"ppat.1008317.ref051","doi-asserted-by":"crossref","first-page":"561","DOI":"10.1038\/nature10441","article-title":"The crystal structure of dynamin","volume":"477","author":"M.G. Ford","year":"2011","journal-title":"Nature"},{"key":"ppat.1008317.ref052","doi-asserted-by":"crossref","first-page":"e71835","DOI":"10.1371\/journal.pone.0071835","article-title":"Functional mapping of human dynamin-1-like GTPase domain based on x-ray structure analyses","volume":"8","author":"J. Wenger","year":"2013","journal-title":"PLoS One"},{"key":"ppat.1008317.ref053","doi-asserted-by":"crossref","first-page":"3345","DOI":"10.1038\/s41467-018-05523-8","article-title":"Structural basis for membrane tethering by a bacterial dynamin-like pair","volume":"9","author":"J. Liu","year":"2018","journal-title":"Nat Commun"},{"key":"ppat.1008317.ref054","doi-asserted-by":"crossref","first-page":"2578","DOI":"10.1091\/mbc.12.9.2578","article-title":"Dynamin GTPase domain mutants block endocytic vesicle formation at morphologically distinct stages","volume":"12","author":"H. Damke","year":"2001","journal-title":"Mol Biol Cell"},{"key":"ppat.1008317.ref055","doi-asserted-by":"crossref","first-page":"e1005756","DOI":"10.1371\/journal.ppat.1005756","article-title":"Static Clathrin Assemblies at the Peripheral Vacuole-Plasma Membrane Interface of the Parasitic Protozoan Giardia lamblia","volume":"12","author":"J.P. Zumthor","year":"2016","journal-title":"PLoS Pathog"},{"key":"ppat.1008317.ref056","doi-asserted-by":"crossref","first-page":"1789","DOI":"10.1091\/mbc.11.5.1789","article-title":"Stage-specific expression and targeting of cyst wall protein-green fluorescent protein chimeras in Giardia","volume":"11","author":"A.B. Hehl","year":"2000","journal-title":"Mol Biol Cell"},{"key":"ppat.1008317.ref057","doi-asserted-by":"crossref","first-page":"e1001670","DOI":"10.1371\/journal.pbio.1001670","article-title":"NECAP 1 regulates AP-2 interactions to control vesicle size, number, and cargo during clathrin-mediated endocytosis","volume":"11","author":"B. Ritter","year":"2013","journal-title":"PLoS Biol"},{"key":"ppat.1008317.ref058","doi-asserted-by":"crossref","first-page":"51","DOI":"10.1038\/nmeth.3179","article-title":"Directed evolution of APEX2 for electron microscopy and proximity labeling","volume":"12","author":"S.S. Lam","year":"2015","journal-title":"Nat Methods"},{"key":"ppat.1008317.ref059","doi-asserted-by":"crossref","first-page":"1143","DOI":"10.1038\/nbt.2375","article-title":"Engineered ascorbate peroxidase as a genetically encoded reporter for electron microscopy","volume":"30","author":"J.D. Martell","year":"2012","journal-title":"Nat Biotechnol"},{"key":"ppat.1008317.ref060","doi-asserted-by":"crossref","first-page":"317","DOI":"10.1042\/bj3640317","article-title":"Cardiolipin prevents rate-dependent uncoupling and provides osmotic stability in yeast mitochondria","volume":"364","author":"V. Koshkin","year":"2002","journal-title":"Biochem J"},{"key":"ppat.1008317.ref061","doi-asserted-by":"crossref","first-page":"3","DOI":"10.1016\/j.biochi.2013.11.018","article-title":"Highly divergent mitochondrion-related organelles in anaerobic parasitic protozoa","volume":"100","author":"T. Makiuchi","year":"2014","journal-title":"Biochimie"},{"key":"ppat.1008317.ref062","doi-asserted-by":"crossref","first-page":"215","DOI":"10.1016\/j.exppara.2008.07.009","article-title":"Cardiolipin, a lipid found in mitochondria, hydrogenosomes and bacteria was not detected in Giardia lamblia","volume":"120","author":"A. Rosa Ide","year":"2008","journal-title":"Exp Parasitol"},{"key":"ppat.1008317.ref063","doi-asserted-by":"crossref","first-page":"1035","DOI":"10.1016\/j.bbamem.2018.01.017","article-title":"Cardiolipin dynamics and binding to conserved residues in the mitochondrial ADP\/ATP carrier","volume":"1860","author":"A.L. Duncan","year":"2018","journal-title":"Biochim Biophys Acta Biomembr"},{"key":"ppat.1008317.ref064","doi-asserted-by":"crossref","first-page":"1036","DOI":"10.1016\/j.bbamcr.2007.12.009","article-title":"Novel tyrosine phosphorylated and cardiolipin-binding protein CLPABP functions as mitochondrial RNA granule","volume":"1783","author":"E. Sano","year":"2008","journal-title":"Biochim Biophys Acta"},{"key":"ppat.1008317.ref065","doi-asserted-by":"crossref","first-page":"1249","DOI":"10.1016\/j.bbrc.2017.11.112","article-title":"Role of N-myristoylation in stability and subcellular localization of the CLPABP protein","volume":"495","author":"A. Maeda","year":"2018","journal-title":"Biochem Biophys Res Commun"},{"key":"ppat.1008317.ref066","doi-asserted-by":"crossref","first-page":"409","DOI":"10.1146\/annurev.micro.62.081307.162826","article-title":"Mitochondrion-related organelles in eukaryotic protists","volume":"64","author":"A.M. Shiflett","year":"2010","journal-title":"Annu Rev Microbiol"},{"key":"ppat.1008317.ref067","doi-asserted-by":"crossref","first-page":"13859","DOI":"10.1038\/ncomms13859","article-title":"Cyst-Wall-Protein-1 is fundamental for Golgi-like organelle neogenesis and cyst-wall biosynthesis in Giardia lamblia","volume":"7","author":"J.A. Ebneter","year":"2016","journal-title":"Nat Commun"},{"key":"ppat.1008317.ref068","doi-asserted-by":"crossref","first-page":"119","DOI":"10.1091\/mbc.E18-09-0605","article-title":"Robust and stable transcriptional repression in Giardia using CRISPRi","volume":"30","author":"S.G. McInally","year":"2019","journal-title":"Mol Biol Cell"},{"key":"ppat.1008317.ref069","doi-asserted-by":"crossref","first-page":"e0213594","DOI":"10.1371\/journal.pone.0213594","article-title":"Development of CRISPR\/Cas9-mediated gene disruption systems in Giardia lamblia","volume":"14","author":"Z.Q. Lin","year":"2019","journal-title":"PLoS One"},{"key":"ppat.1008317.ref070","doi-asserted-by":"crossref","first-page":"57","DOI":"10.1111\/j.1600-0854.2007.00657.x","article-title":"The single dynamin family protein in the primitive protozoan Giardia lamblia is essential for stage conversion and endocytic transport","volume":"9","author":"V. Gaechter","year":"2008","journal-title":"Traffic"},{"key":"ppat.1008317.ref071","doi-asserted-by":"crossref","first-page":"383","DOI":"10.1016\/S0959-440X(99)80052-9","article-title":"Topological characteristics of helical repeat proteins","volume":"9","author":"M.R. Groves","year":"1999","journal-title":"Curr Opin Struct Biol"},{"key":"ppat.1008317.ref072","doi-asserted-by":"crossref","first-page":"1203","DOI":"10.1515\/hsz-2012-0185","article-title":"Structure and function of bacterial dynamin-like proteins","volume":"393","author":"M. Bramkamp","year":"2012","journal-title":"Biol Chem"},{"key":"ppat.1008317.ref073","doi-asserted-by":"crossref","first-page":"646","DOI":"10.1016\/j.bbamcr.2014.12.034","article-title":"The giardial ENTH protein participates in lysosomal protein trafficking and endocytosis","volume":"1853","author":"C. Feliziani","year":"2015","journal-title":"Biochim Biophys Acta"},{"key":"ppat.1008317.ref074","doi-asserted-by":"crossref","first-page":"3516","DOI":"10.1128\/IAI.58.11.3516-3522.1990","article-title":"Excystation of in vitro-derived Giardia lamblia cysts","volume":"58","author":"S.E. Boucher","year":"1990","journal-title":"Infect Immun"},{"key":"ppat.1008317.ref075","doi-asserted-by":"crossref","first-page":"1566","DOI":"10.1128\/EC.00100-10","article-title":"The transcriptional response to encystation stimuli in Giardia lamblia is restricted to a small set of genes","volume":"9","author":"L. Morf","year":"2010","journal-title":"Eukaryot Cell"},{"key":"ppat.1008317.ref076","doi-asserted-by":"crossref","first-page":"2846","DOI":"10.1242\/jcs.049411","article-title":"Neogenesis and maturation of transient Golgi-like cisternae in a simple eukaryote","volume":"122","author":"S. Stefanic","year":"2009","journal-title":"J Cell Sci"},{"key":"ppat.1008317.ref077","doi-asserted-by":"crossref","first-page":"e1000835","DOI":"10.1371\/journal.ppat.1000835","article-title":"Selective condensation drives partitioning and sequential secretion of cyst wall proteins in differentiating Giardia lamblia","volume":"6","author":"C. Konrad","year":"2010","journal-title":"PLoS Pathog"},{"key":"ppat.1008317.ref078","doi-asserted-by":"crossref","first-page":"D447","DOI":"10.1093\/nar\/gkv1145","article-title":"2016 update of the PRIDE database and its related tools","volume":"44","author":"J.A. Vizcaino","year":"2016","journal-title":"Nucleic Acids Res"},{"key":"ppat.1008317.ref079","doi-asserted-by":"crossref","first-page":"676","DOI":"10.1038\/nmeth.2019","article-title":"Fiji: an open-source platform for biological-image analysis","volume":"9","author":"J. Schindelin","year":"2012","journal-title":"Nat Methods"},{"key":"ppat.1008317.ref080","doi-asserted-by":"crossref","first-page":"W695","DOI":"10.1093\/nar\/gkq313","article-title":"A new bioinformatics analysis tools framework at EMBL-EBI","volume":"38","author":"M. Goujon","year":"2010","journal-title":"Nucleic Acids Res"},{"key":"ppat.1008317.ref081","doi-asserted-by":"crossref","first-page":"539","DOI":"10.1038\/msb.2011.75","article-title":"Fast, scalable generation of high-quality protein multiple sequence alignments using Clustal Omega","volume":"7","author":"F. Sievers","year":"2011","journal-title":"Mol Syst Biol"},{"key":"ppat.1008317.ref082","doi-asserted-by":"crossref","first-page":"99","DOI":"10.1038\/nrm2328","article-title":"Membrane recognition by phospholipid-binding domains","volume":"9","author":"M.A. Lemmon","year":"2008","journal-title":"Nat Rev Mol Cell Biol"},{"key":"ppat.1008317.ref083","doi-asserted-by":"crossref","first-page":"231","DOI":"10.1007\/978-1-61779-588-6_10","article-title":"Methods of protein structure comparison","volume":"857","author":"I. Kufareva","year":"2012","journal-title":"Methods Mol Biol"},{"key":"ppat.1008317.ref084","doi-asserted-by":"crossref","first-page":"352","DOI":"10.1016\/j.str.2014.12.009","article-title":"Comparison of Saccharomyces cerevisiae F-BAR domain structures reveals a conserved inositol phosphate binding site","volume":"23","author":"K. Moravcevic","year":"2015","journal-title":"Structure"},{"key":"ppat.1008317.ref085","doi-asserted-by":"crossref","first-page":"1229","DOI":"10.1111\/j.1558-5646.1981.tb04991.x","article-title":"Evolutionary Trees from Gene Frequencies and Quantitative Characters: Finding Maximum Likelihood Estimates","volume":"35","author":"J. Felsenstein","year":"1981","journal-title":"Evolution"},{"key":"ppat.1008317.ref086","doi-asserted-by":"crossref","first-page":"1427","DOI":"10.1098\/rstb.2005.1669","article-title":"Using the quantitative genetic threshold model for inferences between and within species","volume":"360","author":"J. Felsenstein","year":"2005","journal-title":"Philos Trans R Soc Lond B Biol Sci"},{"key":"ppat.1008317.ref087","article-title":"Interactive Tree Of Life (iTOL) v4: recent updates and new developments","author":"I. Letunic","year":"2019","journal-title":"Nucleic Acids Res"}],"updated-by":[{"DOI":"10.1371\/journal.ppat.1008317","type":"new_version","label":"New version","source":"publisher","updated":{"date-parts":[[2020,3,5]],"date-time":"2020-03-05T00:00:00Z","timestamp":1583366400000}}],"container-title":["PLOS Pathogens"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/dx.plos.org\/10.1371\/journal.ppat.1008317","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,7,31]],"date-time":"2024-07-31T21:16:12Z","timestamp":1722460572000},"score":1,"resource":{"primary":{"URL":"https:\/\/dx.plos.org\/10.1371\/journal.ppat.1008317"}},"subtitle":[],"editor":[{"given":"William A.","family":"Petri","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2020,2,24]]},"references-count":87,"journal-issue":{"issue":"2","published-online":{"date-parts":[[2020,2,24]]}},"URL":"https:\/\/doi.org\/10.1371\/journal.ppat.1008317","relation":{"has-preprint":[{"id-type":"doi","id":"10.1101\/741348","asserted-by":"object"}]},"ISSN":["1553-7374"],"issn-type":[{"value":"1553-7374","type":"electronic"}],"subject":[],"published":{"date-parts":[[2020,2,24]]}}}