{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,7]],"date-time":"2026-10-07T22:23:07Z","timestamp":1791411787517,"version":"4.3.3"},"reference-count":94,"publisher":"Public Library of Science (PLoS)","issue":"8","license":[{"start":{"date-parts":[[2020,8,11]],"date-time":"2020-08-11T00:00:00Z","timestamp":1597104000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["www.plospathogens.org"],"crossmark-restriction":false},"short-container-title":["PLoS Pathog"],"DOI":"10.1371\/journal.ppat.1008665","type":"journal-article","created":{"date-parts":[[2020,8,11]],"date-time":"2020-08-11T13:40:36Z","timestamp":1597153236000},"page":"e1008665","update-policy":"https:\/\/doi.org\/10.1371\/journal.ppat.corrections_policy","source":"Crossref","is-referenced-by-count":69,"title":["Structural and functional evaluation of de novo-designed, two-component nanoparticle carriers for HIV Env trimer immunogens"],"prefix":"10.1371","volume":"16","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-9452-8954","authenticated-orcid":true,"given":"Aleksandar","family":"Antanasijevic","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9792-7149","authenticated-orcid":true,"given":"George","family":"Ueda","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Philip J. M.","family":"Brouwer","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4793-7258","authenticated-orcid":true,"given":"Jeffrey","family":"Copps","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6989-639X","authenticated-orcid":true,"given":"Deli","family":"Huang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2547-968X","authenticated-orcid":true,"given":"Joel D.","family":"Allen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3364-3083","authenticated-orcid":true,"given":"Christopher A.","family":"Cottrell","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anila","family":"Yasmeen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2020-2991","authenticated-orcid":true,"given":"Leigh M.","family":"Sewall","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ilja","family":"Bontjer","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thomas J.","family":"Ketas","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4745-8057","authenticated-orcid":true,"given":"Hannah L.","family":"Turner","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4531-9907","authenticated-orcid":true,"given":"Zachary T.","family":"Berndsen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David C.","family":"Montefiori","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Per Johan","family":"Klasse","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1072-2694","authenticated-orcid":true,"given":"Max","family":"Crispin","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4769-6311","authenticated-orcid":true,"given":"David","family":"Nemazee","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"John P.","family":"Moore","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2324-8573","authenticated-orcid":true,"given":"Rogier W.","family":"Sanders","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2978-4692","authenticated-orcid":true,"given":"Neil P.","family":"King","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7896-6217","authenticated-orcid":true,"given":"David","family":"Baker","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7153-3769","authenticated-orcid":true,"given":"Andrew B.","family":"Ward","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"340","published-online":{"date-parts":[[2020,8,11]]},"reference":[{"issue":"1","key":"ppat.1008665.ref001","doi-asserted-by":"crossref","first-page":"54","DOI":"10.1021\/acsnano.6b07343","article-title":"Immunological Principles Guiding the Rational Design of Particles for Vaccine Delivery","volume":"11","author":"KT Gause","year":"2017","journal-title":"ACS Nano"},{"issue":"1","key":"ppat.1008665.ref002","doi-asserted-by":"crossref","first-page":"1655","DOI":"10.1038\/s41467-017-01549-6","article-title":"Structure-based design of native-like HIV-1 envelope trimers to silence non-neutralizing epitopes and eliminate CD4 binding","volume":"8","author":"DW Kulp","year":"2017","journal-title":"Nat Commun"},{"issue":"3","key":"ppat.1008665.ref003","doi-asserted-by":"crossref","first-page":"483","DOI":"10.1016\/j.immuni.2016.08.016","article-title":"HIV Vaccine Design to Target Germline Precursors of Glycan-Dependent Broadly Neutralizing Antibodies","volume":"45","author":"JM Steichen","year":"2016","journal-title":"Immunity"},{"issue":"3","key":"ppat.1008665.ref004","doi-asserted-by":"crossref","first-page":"567","DOI":"10.1016\/j.cell.2019.06.030","article-title":"Antibody Lineages with Vaccine-Induced Antigen-Binding Hotspots Develop Broad HIV Neutralization","volume":"178","author":"R Kong","year":"2019","journal-title":"Cell"},{"issue":"8","key":"ppat.1008665.ref005","doi-asserted-by":"crossref","first-page":"1805","DOI":"10.1016\/j.celrep.2017.07.077","article-title":"Improving the Immunogenicity of Native-like HIV-1 Envelope Trimers by Hyperstabilization","volume":"20","author":"A Torrents de la Pena","year":"2017","journal-title":"Cell Rep"},{"issue":"9","key":"ppat.1008665.ref006","doi-asserted-by":"crossref","first-page":"e1003618","DOI":"10.1371\/journal.ppat.1003618","article-title":"A next-generation cleaved, soluble HIV-1 Env trimer, BG505 SOSIP.664 gp140, expresses multiple epitopes for broadly neutralizing but not non-neutralizing antibodies","volume":"9","author":"RW Sanders","year":"2013","journal-title":"PLoS Pathog"},{"issue":"4","key":"ppat.1008665.ref007","doi-asserted-by":"crossref","first-page":"539","DOI":"10.1016\/j.celrep.2015.03.047","article-title":"Cleavage-independent HIV-1 Env trimers engineered as soluble native spike mimetics for vaccine design","volume":"11","author":"SK Sharma","year":"2015","journal-title":"Cell Rep"},{"issue":"1","key":"ppat.1008665.ref008","doi-asserted-by":"crossref","first-page":"2355","DOI":"10.1038\/s41467-019-10262-5","article-title":"Structure and immunogenicity of a stabilized HIV-1 envelope trimer based on a group-M consensus sequence","volume":"10","author":"K Sliepen","year":"2019","journal-title":"Nat Commun"},{"issue":"5","key":"ppat.1008665.ref009","doi-asserted-by":"crossref","first-page":"855","DOI":"10.1016\/j.immuni.2018.04.029","article-title":"HIV-1 Vaccines Based on Antibody Identification, B Cell Ontogeny, and Epitope Structure","volume":"48","author":"PD Kwong","year":"2018","journal-title":"Immunity"},{"issue":"11","key":"ppat.1008665.ref010","doi-asserted-by":"crossref","first-page":"eaau6769","DOI":"10.1126\/sciadv.aau6769","article-title":"HIV-1 vaccine design through minimizing envelope metastability","volume":"4","author":"L He","year":"2018","journal-title":"Sci Adv"},{"issue":"5","key":"ppat.1008665.ref011","doi-asserted-by":"crossref","first-page":"915","DOI":"10.1016\/j.immuni.2019.10.008","article-title":"Vaccination with Glycan-Modified HIV NFL Envelope Trimer-Liposomes Elicits Broadly Neutralizing Antibodies to Multiple Sites of Vulnerability","volume":"51","author":"V Dubrovskaya","year":"2019","journal-title":"Immunity"},{"issue":"7","key":"ppat.1008665.ref012","doi-asserted-by":"crossref","first-page":"1702","DOI":"10.1016\/j.cell.2015.11.056","article-title":"Immunogenicity of Stabilized HIV-1 Envelope Trimers with Reduced Exposure of Non-neutralizing Epitopes","volume":"163","author":"SW de Taeye","year":"2015","journal-title":"Cell"},{"issue":"4","key":"ppat.1008665.ref013","doi-asserted-by":"crossref","first-page":"e0215163","DOI":"10.1371\/journal.pone.0215163","article-title":"Consistent elicitation of cross-clade HIV-neutralizing responses achieved in guinea pigs after fusion peptide priming by repetitive envelope trimer boosting","volume":"14","author":"C Cheng","year":"2019","journal-title":"PLoS One"},{"issue":"6","key":"ppat.1008665.ref014","doi-asserted-by":"crossref","first-page":"1073","DOI":"10.1016\/j.immuni.2017.05.007","article-title":"Elicitation of Robust Tier 2 Neutralizing Antibody Responses in Nonhuman Primates by HIV Envelope Trimer Immunization Using Optimized Approaches","volume":"46","author":"M Pauthner","year":"2017","journal-title":"Immunity"},{"issue":"6","key":"ppat.1008665.ref015","doi-asserted-by":"crossref","first-page":"857","DOI":"10.1038\/s41591-018-0042-6","article-title":"Epitope-based vaccine design yields fusion peptide-directed antibodies that neutralize diverse strains of HIV-1","volume":"24","author":"K Xu","year":"2018","journal-title":"Nat Med"},{"issue":"9","key":"ppat.1008665.ref016","doi-asserted-by":"crossref","first-page":"e1005864","DOI":"10.1371\/journal.ppat.1005864","article-title":"Sequential and Simultaneous Immunization of Rabbits with HIV-1 Envelope Glycoprotein SOSIP.664 Trimers from Clades A, B and C","volume":"12","author":"PJ Klasse","year":"2016","journal-title":"PLoS Pathog"},{"issue":"7","key":"ppat.1008665.ref017","doi-asserted-by":"crossref","first-page":"2264","DOI":"10.1016\/j.xphs.2019.01.033","article-title":"Developability Assessment of Physicochemical Properties and Stability Profiles of HIV-1 BG505 SOSIP.664 and BG505 SOSIP.v4.1-GT1.1 gp140 Envelope Glycoprotein Trimers as Candidate Vaccine Antigens","volume":"108","author":"N Whitaker","year":"2019","journal-title":"J Pharm Sci"},{"issue":"4","key":"ppat.1008665.ref018","doi-asserted-by":"crossref","first-page":"885","DOI":"10.1002\/bit.26498","article-title":"cGMP production and analysis of BG505 SOSIP.664, an extensively glycosylated, trimeric HIV-1 envelope glycoprotein vaccine candidate","volume":"115","author":"AK Dey","year":"2018","journal-title":"Biotechnol Bioeng"},{"issue":"3","key":"ppat.1008665.ref019","article-title":"Nanoparticle Vaccines for Inducing HIV-1 Neutralizing Antibodies","volume":"7","author":"M Brinkkemper","year":"2019","journal-title":"Vaccines (Basel)"},{"issue":"11","key":"ppat.1008665.ref020","doi-asserted-by":"crossref","first-page":"787","DOI":"10.1038\/nri2868","article-title":"Vaccine delivery: a matter of size, geometry, kinetics and molecular patterns","volume":"10","author":"MF Bachmann","year":"2010","journal-title":"Nat Rev Immunol"},{"issue":"12","key":"ppat.1008665.ref021","doi-asserted-by":"crossref","first-page":"6099","DOI":"10.4049\/jimmunol.170.12.6099","article-title":"Polyvalent antigens stabilize B cell antigen receptor surface signaling microdomains","volume":"170","author":"R Thyagarajan","year":"2003","journal-title":"J Immunol"},{"issue":"6","key":"ppat.1008665.ref022","doi-asserted-by":"crossref","first-page":"651","DOI":"10.1038\/cr.2014.65","article-title":"No receptor stands alone: IgG B-cell receptor intrinsic and extrinsic mechanisms contribute to antibody memory","volume":"24","author":"Y Xu","year":"2014","journal-title":"Cell Res"},{"issue":"10","key":"ppat.1008665.ref023","doi-asserted-by":"crossref","first-page":"3671","DOI":"10.1073\/pnas.73.10.3671","article-title":"Molecular determinants of immunogenicity: the immunon model of immune response","volume":"73","author":"HM Dintzis","year":"1976","journal-title":"Proc Natl Acad Sci U S A"},{"issue":"10","key":"ppat.1008665.ref024","doi-asserted-by":"crossref","first-page":"680","DOI":"10.1016\/j.it.2016.07.007","article-title":"Antigen Receptor Nanoclusters: Small Units with Big Functions","volume":"37","author":"W Liu","year":"2016","journal-title":"Trends Immunol"},{"key":"ppat.1008665.ref025","article-title":"Nanoparticle size influences antigen retention and presentation in lymph node follicles for humoral immunity","author":"YN Zhang","year":"2019","journal-title":"Nano Lett"},{"issue":"6427","key":"ppat.1008665.ref026","doi-asserted-by":"crossref","first-page":"649","DOI":"10.1126\/science.aat9120","article-title":"Innate immune recognition of glycans targets HIV nanoparticle immunogens to germinal centers","volume":"363","author":"T Tokatlian","year":"2019","journal-title":"Science"},{"issue":"5","key":"ppat.1008665.ref027","doi-asserted-by":"crossref","first-page":"427","DOI":"10.1038\/ni.1856","article-title":"Capture of influenza by medullary dendritic cells via SIGN-R1 is essential for humoral immunity in draining lymph nodes","volume":"11","author":"SF Gonzalez","year":"2010","journal-title":"Nat Immunol"},{"issue":"5","key":"ppat.1008665.ref028","doi-asserted-by":"crossref","first-page":"1404","DOI":"10.1002\/eji.200737984","article-title":"Nanoparticles target distinct dendritic cell populations according to their size","volume":"38","author":"V Manolova","year":"2008","journal-title":"Eur J Immunol"},{"issue":"10","key":"ppat.1008665.ref029","doi-asserted-by":"crossref","first-page":"1159","DOI":"10.1038\/nbt1332","article-title":"Exploiting lymphatic transport and complement activation in nanoparticle vaccines","volume":"25","author":"ST Reddy","year":"2007","journal-title":"Nat Biotechnol"},{"issue":"9","key":"ppat.1008665.ref030","doi-asserted-by":"crossref","first-page":"992","DOI":"10.1038\/ni1494","article-title":"Subcapsular encounter and complement-dependent transport of immune complexes by lymph node B cells","volume":"8","author":"TG Phan","year":"2007","journal-title":"Nat Immunol"},{"issue":"1","key":"ppat.1008665.ref031","doi-asserted-by":"crossref","first-page":"26","DOI":"10.1016\/j.jconrel.2006.01.006","article-title":"In vivo targeting of dendritic cells in lymph nodes with poly(propylene sulfide) nanoparticles","volume":"112","author":"ST Reddy","year":"2006","journal-title":"J Control Release"},{"key":"ppat.1008665.ref032","doi-asserted-by":"crossref","first-page":"322","DOI":"10.1016\/j.jconrel.2019.11.035","article-title":"Critical design criteria for engineering a nanoparticulate HIV-1 vaccine","volume":"317","author":"S Thalhauser","year":"2019","journal-title":"J Control Release"},{"issue":"5","key":"ppat.1008665.ref033","doi-asserted-by":"crossref","first-page":"1153","DOI":"10.1016\/j.cell.2019.04.012","article-title":"Slow Delivery Immunization Enhances HIV Neutralizing Antibody and Germinal Center Responses via Modulation of Immunodominance","volume":"177","author":"KM Cirelli","year":"2019","journal-title":"Cell"},{"issue":"6","key":"ppat.1008665.ref034","doi-asserted-by":"crossref","first-page":"1420","DOI":"10.1016\/j.cell.2019.01.046","article-title":"Induction of Potent Neutralizing Antibody Responses by a Designed Protein Nanoparticle Vaccine for Respiratory Syncytial Virus","volume":"176","author":"J Marcandalli","year":"2019","journal-title":"Cell"},{"issue":"6297","key":"ppat.1008665.ref035","doi-asserted-by":"crossref","first-page":"389","DOI":"10.1126\/science.aaf8818","article-title":"Accurate design of megadalton-scale two-component icosahedral protein complexes","volume":"353","author":"JB Bale","year":"2016","journal-title":"Science"},{"issue":"7503","key":"ppat.1008665.ref036","doi-asserted-by":"crossref","first-page":"103","DOI":"10.1038\/nature13404","article-title":"Accurate design of co-assembling multi-component protein nanomaterials","volume":"510","author":"NP King","year":"2014","journal-title":"Nature"},{"issue":"1","key":"ppat.1008665.ref037","doi-asserted-by":"crossref","first-page":"4272","DOI":"10.1038\/s41467-019-12080-1","article-title":"Enhancing and shaping the immunogenicity of native-like HIV-1 envelope trimers with a two-component protein nanoparticle","volume":"10","author":"PJM Brouwer","year":"2019","journal-title":"Nat Commun"},{"key":"ppat.1008665.ref038","article-title":"Tailored Design of Protein Nanoparticle Scaffolds for Multivalent Presentation of Viral Glycoprotein Antigens","author":"G Ueda","year":"2020","journal-title":"bioRxiv"},{"issue":"7","key":"ppat.1008665.ref039","doi-asserted-by":"crossref","first-page":"602","DOI":"10.1038\/89638","article-title":"Computer-based redesign of a protein folding pathway","volume":"8","author":"S Nauli","year":"2001","journal-title":"Nat Struct Biol"},{"issue":"19","key":"ppat.1008665.ref040","doi-asserted-by":"crossref","first-page":"10383","DOI":"10.1073\/pnas.97.19.10383","article-title":"Native protein sequences are close to optimal for their structures","volume":"97","author":"B Kuhlman","year":"2000","journal-title":"Proc Natl Acad Sci U S A"},{"issue":"45","key":"ppat.1008665.ref041","doi-asserted-by":"crossref","first-page":"17656","DOI":"10.1073\/pnas.0702626104","article-title":"Prediction of the structure of symmetrical protein assemblies","volume":"104","author":"I Andre","year":"2007","journal-title":"Proc Natl Acad Sci U S A"},{"issue":"2","key":"ppat.1008665.ref042","doi-asserted-by":"crossref","first-page":"175","DOI":"10.1002\/syn.890060209","article-title":"The benzodiazepine receptor inverse agonist DMCM decreases serotonergic transmission in rat hippocampus: an in vivo electrophysiological study","volume":"6","author":"A Lista","year":"1990","journal-title":"Synapse"},{"issue":"2","key":"ppat.1008665.ref043","doi-asserted-by":"crossref","DOI":"10.3390\/v11020159","article-title":"Induction of Tier 1 HIV Neutralizing Antibodies by Envelope Trimers Incorporated into a Replication Competent Vesicular Stomatitis Virus Vector","volume":"11","author":"CA Bresk","year":"2019","journal-title":"Viruses"},{"issue":"14","key":"ppat.1008665.ref044","doi-asserted-by":"crossref","DOI":"10.1128\/JVI.00415-17","article-title":"Dense Array of Spikes on HIV-1 Virion Particles","volume":"91","author":"A Stano","year":"2017","journal-title":"J Virol"},{"issue":"7762","key":"ppat.1008665.ref045","doi-asserted-by":"crossref","first-page":"468","DOI":"10.1038\/s41586-019-1250-z","article-title":"Immunization expands B cells specific to HIV-1 V3 glycan in mice and macaques","volume":"570","author":"A Escolano","year":"2019","journal-title":"Nature"},{"issue":"16","key":"ppat.1008665.ref046","doi-asserted-by":"crossref","DOI":"10.1128\/JVI.00443-17","article-title":"Covalent Linkage of HIV-1 Trimers to Synthetic Liposomes Elicits Improved B Cell and Antibody Responses","volume":"91","author":"S Bale","year":"2017","journal-title":"J Virol"},{"issue":"5","key":"ppat.1008665.ref047","doi-asserted-by":"crossref","first-page":"804","DOI":"10.1016\/j.immuni.2017.04.021","article-title":"Particulate Array of Well-Ordered HIV Clade C Env Trimers Elicits Neutralizing Antibodies that Display a Unique V2 Cap Approach","volume":"46","author":"P Martinez-Murillo","year":"2017","journal-title":"Immunity17 e7"},{"issue":"9","key":"ppat.1008665.ref048","doi-asserted-by":"crossref","first-page":"1986","DOI":"10.1016\/j.celrep.2016.04.078","article-title":"High-Density Array of Well-Ordered HIV-1 Spikes on Synthetic Liposomal Nanoparticles Efficiently Activate B Cells","volume":"15","author":"J Ingale","year":"2016","journal-title":"Cell Rep"},{"issue":"1","key":"ppat.1008665.ref049","doi-asserted-by":"crossref","first-page":"16527","DOI":"10.1038\/s41598-018-34853-2","article-title":"Enhancing Humoral Responses Against HIV Envelope Trimers via Nanoparticle Delivery with Stabilized Synthetic Liposomes","volume":"8","author":"T Tokatlian","year":"2018","journal-title":"Sci Rep"},{"issue":"5","key":"ppat.1008665.ref050","doi-asserted-by":"crossref","first-page":"e0152038","DOI":"10.1371\/journal.pone.0152038","article-title":"Comparative Immunogenicity of HIV-1 gp140 Vaccine Delivered by Parenteral, and Mucosal Routes in Female Volunteers; MUCOVAC2, A Randomized Two Centre Study","volume":"11","author":"CA Cosgrove","year":"2016","journal-title":"PLoS One"},{"key":"ppat.1008665.ref051","doi-asserted-by":"crossref","first-page":"43","DOI":"10.1016\/j.jconrel.2014.03.018","article-title":"Polymeric penetration enhancers promote humoral immune responses to mucosal vaccines","volume":"183","author":"K Klein","year":"2014","journal-title":"J Control Release"},{"key":"ppat.1008665.ref052","article-title":"Neutralizing antibody induction by HIV-1 Envelope glycoprotein SOSIP trimers on iron oxide nanoparticles may be impaired by mannose binding lectin","author":"RP Ringe","year":"2019","journal-title":"J Virol"},{"issue":"1","key":"ppat.1008665.ref053","doi-asserted-by":"crossref","first-page":"911","DOI":"10.1016\/j.xphs.2019.10.059","article-title":"Presentation of HIV-1 Envelope Trimers on the Surface of Silica Nanoparticles","volume":"109","author":"S Thalhauser","year":"2020","journal-title":"J Pharm Sci"},{"issue":"6244","key":"ppat.1008665.ref054","doi-asserted-by":"crossref","first-page":"156","DOI":"10.1126\/science.aac5894","article-title":"HIV-1 VACCINES. Priming a broadly neutralizing antibody response to HIV-1 using a germline-targeting immunogen","volume":"349","author":"JG Jardine","year":"2015","journal-title":"Science"},{"issue":"6133","key":"ppat.1008665.ref055","doi-asserted-by":"crossref","first-page":"711","DOI":"10.1126\/science.1234150","article-title":"Rational HIV immunogen design to target specific germline B cell receptors","volume":"340","author":"J Jardine","year":"2013","journal-title":"Science"},{"key":"ppat.1008665.ref056","doi-asserted-by":"crossref","first-page":"12041","DOI":"10.1038\/ncomms12041","article-title":"Presenting native-like trimeric HIV-1 antigens with self-assembling nanoparticles","volume":"7","author":"L He","year":"2016","journal-title":"Nat Commun"},{"key":"ppat.1008665.ref057","doi-asserted-by":"crossref","first-page":"82","DOI":"10.1186\/s12977-015-0210-4","article-title":"Presenting native-like HIV-1 envelope trimers on ferritin nanoparticles improves their immunogenicity","volume":"12","author":"K Sliepen","year":"2015","journal-title":"Retrovirology"},{"issue":"5","key":"ppat.1008665.ref058","doi-asserted-by":"crossref","first-page":"788","DOI":"10.1021\/acsinfecdis.7b00192","article-title":"Two-Component Ferritin Nanoparticles for Multimerization of Diverse Trimeric Antigens","volume":"4","author":"IS Georgiev","year":"2018","journal-title":"ACS Infect Dis"},{"issue":"22","key":"ppat.1008665.ref059","doi-asserted-by":"crossref","DOI":"10.1128\/JVI.01349-18","article-title":"Development of a Pan-H1 Influenza Vaccine","volume":"92","author":"N Darricarrere","year":"2018","journal-title":"J Virol"},{"issue":"7456","key":"ppat.1008665.ref060","doi-asserted-by":"crossref","first-page":"102","DOI":"10.1038\/nature12202","article-title":"Self-assembling influenza nanoparticle vaccines elicit broadly neutralizing H1N1 antibodies","volume":"499","author":"M Kanekiyo","year":"2013","journal-title":"Nature"},{"issue":"4","key":"ppat.1008665.ref061","doi-asserted-by":"crossref","first-page":"507","DOI":"10.1016\/j.chom.2020.03.018","article-title":"Env Exceptionalism: Why Are HIV-1 Env Glycoproteins Atypical Immunogens?","volume":"27","author":"PJ Klasse","journal-title":"Cell Host Microbe. 2020"},{"issue":"4","key":"ppat.1008665.ref062","doi-asserted-by":"crossref","first-page":"302","DOI":"10.1097\/COH.0000000000000549","article-title":"Presentation of HIV-1 envelope glycoprotein trimers on diverse nanoparticle platforms","volume":"14","author":"PJM Brouwer","year":"2019","journal-title":"Curr Opin HIV AIDS"},{"issue":"6","key":"ppat.1008665.ref063","doi-asserted-by":"crossref","DOI":"10.1128\/JVI.01883-19","article-title":"Neutralizing Antibody Induction by HIV-1 Envelope Glycoprotein SOSIP Trimers on Iron Oxide Nanoparticles May Be Impaired by Mannose Binding Lectin","volume":"94","author":"RP Ringe","year":"2020","journal-title":"J Virol"},{"issue":"4","key":"ppat.1008665.ref064","doi-asserted-by":"crossref","first-page":"353","DOI":"10.1038\/nchem.2673","article-title":"Computational design of self-assembling cyclic protein homo-oligomers","volume":"9","author":"JA Fallas","year":"2017","journal-title":"Nat Chem"},{"issue":"9","key":"ppat.1008665.ref065","doi-asserted-by":"crossref","first-page":"2327","DOI":"10.1016\/j.celrep.2016.07.074","article-title":"Holes in the Glycan Shield of the Native HIV Envelope Are a Target of Trimer-Elicited Neutralizing Antibodies","volume":"16","author":"LE McCoy","year":"2016","journal-title":"Cell Rep"},{"issue":"4","key":"ppat.1008665.ref066","doi-asserted-by":"crossref","DOI":"10.1128\/JVI.01656-18","article-title":"Closing and Opening Holes in the Glycan Shield of HIV-1 Envelope Glycoprotein SOSIP Trimers Can Redirect the Neutralizing Antibody Response to the Newly Unmasked Epitopes","volume":"93","author":"RP Ringe","year":"2019","journal-title":"J Virol"},{"key":"ppat.1008665.ref067","article-title":"Targeting HIV Env immunogens to B cell follicles in non-human primates through immune complex or protein nanoparticle formulations","author":"JT Martin","year":"2020","journal-title":"bioRxiv"},{"key":"ppat.1008665.ref068","first-page":"5","article-title":"Automated structure refinement of macromolecular assemblies from cryo-EM maps using Rosetta","author":"RY Wang","year":"2016","journal-title":"Elife"},{"issue":"4","key":"ppat.1008665.ref069","doi-asserted-by":"crossref","first-page":"256","DOI":"10.1107\/S2059798318005119","article-title":"Structural analysis of glycoproteins: building N-linked glycans with Coot","volume":"74","author":"P Emsley","year":"2018","journal-title":"Acta Crystallogr D Struct Biol"},{"key":"ppat.1008665.ref070","doi-asserted-by":"crossref","first-page":"8843","DOI":"10.1038\/ncomms9843","article-title":"Localized reconstruction of subunits from electron cryomicroscopy images of macromolecular complexes","volume":"6","author":"SL Ilca","year":"2015","journal-title":"Nat Commun"},{"issue":"6","key":"ppat.1008665.ref071","doi-asserted-by":"crossref","first-page":"1053","DOI":"10.1016\/j.immuni.2015.11.007","article-title":"Affinity Maturation of a Potent Family of HIV Antibodies Is Primarily Focused on Accommodating or Avoiding Glycans","volume":"43","author":"F Garces","year":"2015","journal-title":"Immunity"},{"issue":"10","key":"ppat.1008665.ref072","doi-asserted-by":"crossref","first-page":"1943","DOI":"10.1016\/j.str.2015.07.020","article-title":"Model Building and Refinement of a Natively Glycosylated HIV-1 Env Protein by High-Resolution Cryoelectron Microscopy","volume":"23","author":"JH Lee","year":"2015","journal-title":"Structure"},{"issue":"10","key":"ppat.1008665.ref073","doi-asserted-by":"crossref","first-page":"4816","DOI":"10.4049\/jimmunol.1202165","article-title":"Anti-HIV B Cell lines as candidate vaccine biosensors","volume":"189","author":"T Ota","year":"2012","journal-title":"J Immunol"},{"issue":"6","key":"ppat.1008665.ref074","doi-asserted-by":"crossref","first-page":"2806","DOI":"10.1128\/JVI.02652-15","article-title":"Structure-Guided Redesign Increases the Propensity of HIV Env To Generate Highly Stable Soluble Trimers","volume":"90","author":"J Guenaga","year":"2015","journal-title":"J Virol"},{"issue":"2","key":"ppat.1008665.ref075","doi-asserted-by":"crossref","first-page":"288","DOI":"10.1016\/j.immuni.2018.07.009","article-title":"Electron-Microscopy-Based Epitope Mapping Defines Specificities of Polyclonal Antibodies Elicited during HIV-1 BG505 Envelope Trimer Immunization","volume":"49","author":"M Bianchi","year":"2018","journal-title":"Immunity"},{"issue":"11","key":"ppat.1008665.ref076","doi-asserted-by":"crossref","first-page":"2695","DOI":"10.1016\/j.celrep.2016.02.058","article-title":"Composition and Antigenic Effects of Individual Glycan Sites of a Trimeric HIV-1 Envelope Glycoprotein","volume":"14","author":"AJ Behrens","year":"2016","journal-title":"Cell Rep"},{"key":"ppat.1008665.ref077","doi-asserted-by":"crossref","first-page":"14954","DOI":"10.1038\/ncomms14954","article-title":"Global site-specific N-glycosylation analysis of HIV envelope glycoprotein","volume":"8","author":"L Cao","year":"2017","journal-title":"Nat Commun"},{"issue":"11","key":"ppat.1008665.ref078","doi-asserted-by":"crossref","first-page":"3249","DOI":"10.1016\/j.celrep.2018.05.046","article-title":"Co-evolution of HIV Envelope and Apex-Targeting Neutralizing Antibody Lineage Provides Benchmarks for Vaccine Design","volume":"23","author":"K Rantalainen","year":"2018","journal-title":"Cell Rep"},{"issue":"9","key":"ppat.1008665.ref079","doi-asserted-by":"crossref","first-page":"2573","DOI":"10.1084\/jem.20161160","article-title":"Design and crystal structure of a native-like HIV-1 envelope trimer that engages multiple broadly neutralizing antibody precursors in vivo","volume":"214","author":"M Medina-Ramirez","year":"2017","journal-title":"J Exp Med"},{"issue":"3","key":"ppat.1008665.ref080","doi-asserted-by":"crossref","first-page":"362","DOI":"10.1038\/s41590-018-0305-x","article-title":"Mosaic nanoparticle display of diverse influenza virus hemagglutinins elicits broad B cell responses","volume":"20","author":"M Kanekiyo","year":"2019","journal-title":"Nat Immunol"},{"issue":"13","key":"ppat.1008665.ref081","doi-asserted-by":"crossref","DOI":"10.1128\/JVI.00381-18","article-title":"Effects of Adjuvants on HIV-1 Envelope Glycoprotein SOSIP Trimers In Vitro","volume":"92","author":"G Ozorowski","year":"2018","journal-title":"J Virol"},{"key":"ppat.1008665.ref082","doi-asserted-by":"crossref","first-page":"8167","DOI":"10.1038\/ncomms9167","article-title":"Antibodies to a conformational epitope on gp41 neutralize HIV-1 by destabilizing the Env spike","volume":"6","author":"JH Lee","year":"2015","journal-title":"Nat Commun"},{"key":"ppat.1008665.ref083","first-page":"7","article-title":"New tools for automated high-resolution cryo-EM structure determination in RELION-3","author":"J Zivanov","year":"2018","journal-title":"Elife"},{"issue":"13","key":"ppat.1008665.ref084","doi-asserted-by":"crossref","first-page":"1605","DOI":"10.1002\/jcc.20084","article-title":"UCSF Chimera\u2014a visualization system for exploratory research and analysis","volume":"25","author":"EF Pettersen","year":"2004","journal-title":"J Comput Chem"},{"issue":"1","key":"ppat.1008665.ref085","doi-asserted-by":"crossref","first-page":"41","DOI":"10.1016\/j.jsb.2005.03.010","article-title":"Automated molecular microscopy: the new Leginon system","volume":"151","author":"C Suloway","year":"2005","journal-title":"J Struct Biol"},{"issue":"4","key":"ppat.1008665.ref086","doi-asserted-by":"crossref","first-page":"331","DOI":"10.1038\/nmeth.4193","article-title":"MotionCor2: anisotropic correction of beam-induced motion for improved cryo-electron microscopy","volume":"14","author":"SQ Zheng","year":"2017","journal-title":"Nat Methods"},{"issue":"3","key":"ppat.1008665.ref087","doi-asserted-by":"crossref","first-page":"290","DOI":"10.1038\/nmeth.4169","article-title":"cryoSPARC: algorithms for rapid unsupervised cryo-EM structure determination","volume":"14","author":"A Punjani","year":"2017","journal-title":"Nat Methods"},{"issue":"10","key":"ppat.1008665.ref088","doi-asserted-by":"crossref","first-page":"943","DOI":"10.1038\/nmeth.3541","article-title":"EMRinger: side chain-directed model and map validation for 3D cryo-electron microscopy","volume":"12","author":"BA Barad","year":"2015","journal-title":"Nat Methods"},{"issue":"1","key":"ppat.1008665.ref089","doi-asserted-by":"crossref","first-page":"293","DOI":"10.1002\/pro.3330","article-title":"MolProbity: More and better reference data for improved all-atom structure validation","volume":"27","author":"CJ Williams","year":"2018","journal-title":"Protein Sci"},{"key":"ppat.1008665.ref090","doi-asserted-by":"crossref","first-page":"41","DOI":"10.1186\/1742-4690-11-41","article-title":"Differential binding of neutralizing and non-neutralizing antibodies to native-like soluble HIV-1 Env trimers, uncleaved Env proteins, and monomeric subunits","volume":"11","author":"A Yasmeen","year":"2014","journal-title":"Retrovirology"},{"issue":"8","key":"ppat.1008665.ref091","doi-asserted-by":"crossref","first-page":"1958","DOI":"10.1016\/j.celrep.2018.07.080","article-title":"Site-Specific Glycosylation of Virion-Derived HIV-1 Env Is Mimicked by a Soluble Trimeric Immunogen","volume":"24","author":"WB Struwe","year":"2018","journal-title":"Cell Rep"},{"issue":"6244","key":"ppat.1008665.ref092","doi-asserted-by":"crossref","first-page":"aac4223","DOI":"10.1126\/science.aac4223","article-title":"HIV-1 VACCINES. HIV-1 neutralizing antibodies induced by native-like envelope trimers","volume":"349","author":"RW Sanders","year":"2015","journal-title":"Science"},{"key":"ppat.1008665.ref093","doi-asserted-by":"crossref","first-page":"395","DOI":"10.1007\/978-1-59745-170-3_26","article-title":"Measuring HIV neutralization in a luciferase reporter gene assay","volume":"485","author":"DC Montefiori","year":"2009","journal-title":"Methods Mol Biol"},{"issue":"1","key":"ppat.1008665.ref094","doi-asserted-by":"crossref","first-page":"95","DOI":"10.1016\/j.jsb.2009.01.002","article-title":"Appion: an integrated, database-driven pipeline to facilitate EM image processing","volume":"166","author":"GC Lander","year":"2009","journal-title":"J Struct Biol"}],"container-title":["PLOS Pathogens"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/dx.plos.org\/10.1371\/journal.ppat.1008665","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2020,8,11]],"date-time":"2020-08-11T13:41:53Z","timestamp":1597153313000},"score":1,"resource":{"primary":{"URL":"https:\/\/dx.plos.org\/10.1371\/journal.ppat.1008665"}},"subtitle":[],"editor":[{"given":"David T.","family":"Evans","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2020,8,11]]},"references-count":94,"journal-issue":{"issue":"8","published-online":{"date-parts":[[2020,8,11]]}},"URL":"https:\/\/doi.org\/10.1371\/journal.ppat.1008665","relation":{"has-preprint":[{"id-type":"doi","id":"10.1101\/2020.01.31.929273","asserted-by":"object"}]},"ISSN":["1553-7374"],"issn-type":[{"value":"1553-7374","type":"electronic"}],"subject":[],"published":{"date-parts":[[2020,8,11]]}}}