{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,25]],"date-time":"2026-09-25T05:32:43Z","timestamp":1790314363737,"version":"4.1.0"},"update-to":[{"DOI":"10.1371\/journal.ppat.1008666","type":"new_version","label":"New version","source":"publisher","updated":{"date-parts":[[2020,12,8]],"date-time":"2020-12-08T00:00:00Z","timestamp":1607385600000}}],"reference-count":110,"publisher":"Public Library of Science (PLoS)","issue":"11","license":[{"start":{"date-parts":[[2020,11,24]],"date-time":"2020-11-24T00:00:00Z","timestamp":1606176000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100000060","name":"National Institute of Allergy and Infectious Diseases","doi-asserted-by":"publisher","award":["P01AI129859"],"award-info":[{"award-number":["P01AI129859"]}],"id":[{"id":"10.13039\/100000060","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000060","name":"National Institute of Allergy and Infectious Diseases","doi-asserted-by":"publisher","award":["P01AI129859"],"award-info":[{"award-number":["P01AI129859"]}],"id":[{"id":"10.13039\/100000060","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000060","name":"National Institute of Allergy and Infectious Diseases","doi-asserted-by":"crossref","award":["P01AI129859"],"award-info":[{"award-number":["P01AI129859"]}],"id":[{"id":"10.13039\/100000060","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/100000060","name":"National Institute of Allergy and Infectious Diseases","doi-asserted-by":"publisher","award":["R01 AI095113"],"award-info":[{"award-number":["R01 AI095113"]}],"id":[{"id":"10.13039\/100000060","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000060","name":"National Institute of Allergy and Infectious Diseases","doi-asserted-by":"publisher","award":["P01 AI094417"],"award-info":[{"award-number":["P01 AI094417"]}],"id":[{"id":"10.13039\/100000060","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000060","name":"National Institute of Allergy and Infectious Diseases","doi-asserted-by":"publisher","award":["R37 AI054292"],"award-info":[{"award-number":["R37 AI054292"]}],"id":[{"id":"10.13039\/100000060","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000060","name":"National Institute of Allergy and Infectious Diseases","doi-asserted-by":"publisher","award":["R01 AI059457"],"award-info":[{"award-number":["R01 AI059457"]}],"id":[{"id":"10.13039\/100000060","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000060","name":"National Institute of Allergy and Infectious Diseases","doi-asserted-by":"publisher","award":["OD023038"],"award-info":[{"award-number":["OD023038"]}],"id":[{"id":"10.13039\/100000060","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000052","name":"NIH Office of the Director","doi-asserted-by":"publisher","award":["U42OD010426"],"award-info":[{"award-number":["U42OD010426"]}],"id":[{"id":"10.13039\/100000052","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000052","name":"NIH Office of the Director","doi-asserted-by":"publisher","award":["P51OD011092"],"award-info":[{"award-number":["P51OD011092"]}],"id":[{"id":"10.13039\/100000052","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000052","name":"NIH Office of the Director","doi-asserted-by":"publisher","award":["P51OD011104"],"award-info":[{"award-number":["P51OD011104"]}],"id":[{"id":"10.13039\/100000052","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100009633","name":"Eunice Kennedy Shriver National Institute of Child Health and Human Development","doi-asserted-by":"publisher","award":["4DP2HD075699"],"award-info":[{"award-number":["4DP2HD075699"]}],"id":[{"id":"10.13039\/100009633","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000865","name":"Bill and Melinda Gates Foundation","doi-asserted-by":"publisher","award":["OPP1033121"],"award-info":[{"award-number":["OPP1033121"]}],"id":[{"id":"10.13039\/100000865","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000865","name":"Bill and Melinda Gates Foundation","doi-asserted-by":"publisher","award":["OPP1108533"],"award-info":[{"award-number":["OPP1108533"]}],"id":[{"id":"10.13039\/100000865","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000865","name":"Bill and Melinda Gates Foundation","doi-asserted-by":"publisher","award":["OPP1152430"],"award-info":[{"award-number":["OPP1152430"]}],"id":[{"id":"10.13039\/100000865","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["www.plospathogens.org"],"crossmark-restriction":false},"short-container-title":["PLoS Pathog"],"abstract":"<jats:p>\n                    Cytomegaloviruses (CMVs) are highly adapted to their host species resulting in strict species specificity. Hence,\n                    <jats:italic>in vivo<\/jats:italic>\n                    examination of all aspects of CMV biology employs animal models using host-specific CMVs. Infection of rhesus macaques (RM) with rhesus CMV (RhCMV) has been established as a representative model for infection of humans with HCMV due to the close evolutionary relationships of both host and virus. However, the only available RhCMV clone that permits genetic modifications is based on the 68\u20131 strain which has been passaged in fibroblasts for decades resulting in multiple genomic changes due to tissue culture adaptations. As a result, 68\u20131 displays reduced viremia in RhCMV-na\u00efve animals and limited shedding compared to non-clonal, low passage isolates. To overcome this limitation, we used sequence information from primary RhCMV isolates to construct a full-length (FL) RhCMV by repairing all mutations affecting open reading frames (ORFs) in the 68\u20131 bacterial artificial chromosome (BAC). Inoculation of adult, immunocompetent, RhCMV-na\u00efve RM with the reconstituted virus resulted in significant viremia in the blood similar to primary isolates of RhCMV and furthermore led to high viral genome copy numbers in many tissues at day 14 post infection. In contrast, viral dissemination was greatly reduced upon deletion of genes also lacking in 68\u20131. Transcriptome analysis of infected tissues further revealed that chemokine-like genes deleted in 68\u20131 are among the most highly expressed viral transcripts both\n                    <jats:italic>in vitro<\/jats:italic>\n                    and\n                    <jats:italic>in vivo<\/jats:italic>\n                    consistent with an important immunomodulatory function of the respective proteins. We conclude that FL-RhCMV displays\n                    <jats:italic>in vitro<\/jats:italic>\n                    and\n                    <jats:italic>in vivo<\/jats:italic>\n                    characteristics of a wildtype virus while being amenable to genetic modifications through BAC recombineering techniques.\n                  <\/jats:p>","DOI":"10.1371\/journal.ppat.1008666","type":"journal-article","created":{"date-parts":[[2020,11,24]],"date-time":"2020-11-24T18:29:11Z","timestamp":1606242551000},"page":"e1008666","update-policy":"https:\/\/doi.org\/10.1371\/journal.ppat.corrections_policy","source":"Crossref","is-referenced-by-count":36,"title":["In vitro and in vivo characterization of a recombinant rhesus cytomegalovirus containing a complete genome"],"prefix":"10.1371","volume":"16","author":[{"given":"Husam","family":"Taher","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5613-3131","authenticated-orcid":true,"given":"Eisa","family":"Mahyari","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Craig","family":"Kreklywich","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Luke S.","family":"Uebelhoer","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Matthew R.","family":"McArdle","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7261-6638","authenticated-orcid":true,"given":"Matilda J.","family":"Mostr\u00f6m","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Amruta","family":"Bhusari","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michael","family":"Nekorchuk","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9520-2040","authenticated-orcid":true,"given":"Xiaofei","family":"E","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9321-0637","authenticated-orcid":true,"given":"Travis","family":"Whitmer","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4209-0409","authenticated-orcid":true,"given":"Elizabeth A.","family":"Scheef","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lesli M.","family":"Sprehe","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8411-646X","authenticated-orcid":true,"given":"Dawn L.","family":"Roberts","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Colette M.","family":"Hughes","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7131-3958","authenticated-orcid":true,"given":"Kerianne A.","family":"Jackson","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9536-463X","authenticated-orcid":true,"given":"Andrea N.","family":"Selseth","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Abigail B.","family":"Ventura","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0054-0854","authenticated-orcid":true,"given":"Hillary C.","family":"Cleveland-Rubeor","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yujuan","family":"Yue","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4927-6991","authenticated-orcid":true,"given":"Kimberli A.","family":"Schmidt","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0830-0211","authenticated-orcid":true,"given":"Jason","family":"Shao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Paul T.","family":"Edlefsen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3369-4662","authenticated-orcid":true,"given":"Jeremy","family":"Smedley","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6096-3018","authenticated-orcid":true,"given":"Timothy F.","family":"Kowalik","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6799-1182","authenticated-orcid":true,"given":"Richard J.","family":"Stanton","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1984-5906","authenticated-orcid":true,"given":"Michael K.","family":"Axthelm","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jacob D.","family":"Estes","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Scott G.","family":"Hansen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Amitinder","family":"Kaur","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7982-674X","authenticated-orcid":true,"given":"Peter A.","family":"Barry","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8092-3080","authenticated-orcid":true,"given":"Benjamin N.","family":"Bimber","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0546-398X","authenticated-orcid":true,"given":"Louis J.","family":"Picker","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Daniel N.","family":"Streblow","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Klaus","family":"Fr\u00fch","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1287-7283","authenticated-orcid":true,"given":"Daniel","family":"Malouli","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"340","published-online":{"date-parts":[[2020,11,24]]},"reference":[{"issue":"4","key":"ppat.1008666.ref001","doi-asserted-by":"crossref","first-page":"202","DOI":"10.1002\/rmv.655","article-title":"Review of cytomegalovirus seroprevalence and demographic characteristics associated with infection","volume":"20","author":"MJ Cannon","year":"2010","journal-title":"Rev Med Virol"},{"key":"ppat.1008666.ref002","first-page":"1","volume-title":"Cytomegaloviruses: From Molecular Pathogenesis to Intervention","author":"SB Boppana","year":"2013","edition":"2"},{"issue":"Supplement_1","key":"ppat.1008666.ref003","doi-asserted-by":"crossref","first-page":"S32","DOI":"10.1093\/infdis\/jiz493","article-title":"Advances in the Development of Therapeutics for Cytomegalovirus Infections","volume":"221","author":"E Acosta","year":"2020","journal-title":"J Infect Dis"},{"issue":"3","key":"ppat.1008666.ref004","doi-asserted-by":"crossref","first-page":"247","DOI":"10.1128\/MR.54.3.247-265.1990","article-title":"Animal cytomegaloviruses","volume":"54","author":"J. Staczek","year":"1990","journal-title":"Microbiol Rev"},{"issue":"11","key":"ppat.1008666.ref005","doi-asserted-by":"crossref","first-page":"e1005288","DOI":"10.1371\/journal.ppat.1005288","article-title":"The Transcription and Translation Landscapes during Human Cytomegalovirus Infection Reveal Novel Host-Pathogen Interactions","volume":"11","author":"O Tirosh","year":"2015","journal-title":"PLoS Pathog"},{"issue":"3","key":"ppat.1008666.ref006","doi-asserted-by":"crossref","first-page":"447","DOI":"10.1016\/j.chom.2018.07.011","article-title":"High-Definition Analysis of Host Protein Stability during Human Cytomegalovirus Infection Reveals Antiviral Factors and Viral Evasion Mechanisms","volume":"24","author":"K Nightingale","year":"2018","journal-title":"Cell Host Microbe"},{"key":"ppat.1008666.ref007","doi-asserted-by":"crossref","DOI":"10.7554\/eLife.49894","article-title":"Human cytomegalovirus interactome analysis identifies degradation hubs, domain associations and viral protein functions","volume":"8","author":"LV Nobre","year":"2019","journal-title":"Elife"},{"issue":"3","key":"ppat.1008666.ref008","doi-asserted-by":"crossref","first-page":"443","DOI":"10.1006\/jmbi.1995.0152","article-title":"Molecular phylogeny and evolutionary timescale for the family of mammalian herpesviruses","volume":"247","author":"DJ McGeoch","year":"1995","journal-title":"J Mol Biol"},{"issue":"11","key":"ppat.1008666.ref009","doi-asserted-by":"crossref","first-page":"2634","DOI":"10.1038\/sj.emboj.7601133","article-title":"Induction of apoptosis limits cytomegalovirus cross-species infection","volume":"25","author":"I Jurak","year":"2006","journal-title":"EMBO J"},{"issue":"17","key":"ppat.1008666.ref010","doi-asserted-by":"crossref","first-page":"8959","DOI":"10.1128\/JVI.01132-12","article-title":"Reevaluation of the coding potential and proteomic analysis of the BAC-derived rhesus cytomegalovirus strain 68\u20131","volume":"86","author":"D Malouli","year":"2012","journal-title":"J Virol"},{"issue":"44","key":"ppat.1008666.ref011","doi-asserted-by":"crossref","first-page":"13645","DOI":"10.1073\/pnas.1511526112","article-title":"Maternal CD4+ T cells protect against severe congenital cytomegalovirus disease in a novel nonhuman primate model of placental cytomegalovirus transmission","volume":"112","author":"KM Bialas","year":"2015","journal-title":"Proc Natl Acad Sci U S A"},{"key":"ppat.1008666.ref012","doi-asserted-by":"crossref","first-page":"52","DOI":"10.1016\/j.coi.2017.06.010","article-title":"CD8+ T cell programming by cytomegalovirus vectors: applications in prophylactic and therapeutic vaccination","volume":"47","author":"K Fruh","year":"2017","journal-title":"Curr Opin Immunol"},{"issue":"7348","key":"ppat.1008666.ref013","doi-asserted-by":"crossref","first-page":"523","DOI":"10.1038\/nature10003","article-title":"Profound early control of highly pathogenic SIV by an effector memory T-cell vaccine","volume":"473","author":"SG Hansen","year":"2011","journal-title":"Nature"},{"issue":"2","key":"ppat.1008666.ref014","doi-asserted-by":"crossref","first-page":"130","DOI":"10.1038\/nm.4473","article-title":"Prevention of tuberculosis in rhesus macaques by a cytomegalovirus-based vaccine","volume":"24","author":"SG Hansen","year":"2018","journal-title":"Nat Med"},{"issue":"1","key":"ppat.1008666.ref015","doi-asserted-by":"crossref","first-page":"e0210252","DOI":"10.1371\/journal.pone.0210252","article-title":"Cytomegalovirus vectors expressing Plasmodium knowlesi antigens induce immune responses that delay parasitemia upon sporozoite challenge","volume":"14","author":"SG Hansen","year":"2019","journal-title":"LoS One"},{"issue":"7469","key":"ppat.1008666.ref016","doi-asserted-by":"crossref","first-page":"100","DOI":"10.1038\/nature12519","article-title":"Immune clearance of highly pathogenic SIV infection","volume":"502","author":"SG Hansen","year":"2013","journal-title":"Nature"},{"issue":"6135","key":"ppat.1008666.ref017","doi-asserted-by":"crossref","first-page":"1237874","DOI":"10.1126\/science.1237874","article-title":"Cytomegalovirus vectors violate CD8+ T cell epitope recognition paradigms","volume":"340","author":"SG Hansen","year":"2013","journal-title":"Science"},{"issue":"6274","key":"ppat.1008666.ref018","doi-asserted-by":"crossref","first-page":"714","DOI":"10.1126\/science.aac9475","article-title":"T cell responses restricted by major histocompatibility complex E","volume":"351","author":"SG Hansen","year":"2016","journal-title":"Science"},{"issue":"501","key":"ppat.1008666.ref019","doi-asserted-by":"crossref","DOI":"10.1126\/scitranslmed.aaw2607","article-title":"A live-attenuated RhCMV\/SIV vaccine shows long-term efficacy against heterologous SIV challenge","volume":"11","author":"SG Hansen","year":"2019","journal-title":"Sci Transl Med"},{"issue":"501","key":"ppat.1008666.ref020","doi-asserted-by":"crossref","DOI":"10.1126\/scitranslmed.aaw2603","article-title":"Enhancing safety of cytomegalovirus-based vaccine vectors by engaging host intrinsic immunity","volume":"11","author":"EE Marshall","year":"2019","journal-title":"Sci Transl Med"},{"issue":"3","key":"ppat.1008666.ref021","doi-asserted-by":"crossref","first-page":"794","DOI":"10.3181\/00379727-145-37897","article-title":"Persistent shedding of cytomegalovirus in the urine of healthy Rhesus monkeys","volume":"145","author":"DM Asher","year":"1974","journal-title":"Proc Soc Exp Biol Med"},{"issue":"9","key":"ppat.1008666.ref022","doi-asserted-by":"crossref","first-page":"5073","DOI":"10.1128\/JVI.77.9.5073-5083.2003","article-title":"Cloning of the full-length rhesus cytomegalovirus genome as an infectious and self-excisable bacterial artificial chromosome for analysis of viral pathogenesis","volume":"77","author":"WL Chang","year":"2003","journal-title":"J Virol"},{"issue":"1","key":"ppat.1008666.ref023","doi-asserted-by":"crossref","first-page":"181","DOI":"10.1016\/j.virol.2007.10.040","article-title":"Protein coding content of the UL)b' region of wild-type rhesus cytomegalovirus","volume":"373","author":"KL Oxford","year":"2008","journal-title":"Virology"},{"issue":"11","key":"ppat.1008666.ref024","doi-asserted-by":"crossref","first-page":"6354","DOI":"10.1128\/JVI.00607-12","article-title":"Patterns of acute rhesus cytomegalovirus (RhCMV) infection predict long-term RhCMV infection","volume":"86","author":"BT Assaf","year":"2012","journal-title":"J Virol"},{"issue":"50","key":"ppat.1008666.ref025","doi-asserted-by":"crossref","first-page":"19950","DOI":"10.1073\/pnas.0811063106","article-title":"Efficient replication of rhesus cytomegalovirus variants in multiple rhesus and human cell types","volume":"105","author":"AE Lilja","year":"2008","journal-title":"Proc Natl Acad Sci U S A"},{"issue":"10","key":"ppat.1008666.ref026","doi-asserted-by":"crossref","first-page":"5105","DOI":"10.1128\/JVI.02631-10","article-title":"Open reading frames carried on UL\/b' are implicated in shedding and horizontal transmission of rhesus cytomegalovirus in rhesus monkeys","volume":"85","author":"KL Oxford","year":"2011","journal-title":"J Virol"},{"issue":"13","key":"ppat.1008666.ref027","doi-asserted-by":"crossref","DOI":"10.1172\/jci.insight.94002","article-title":"Preexisting antibodies can protect against congenital cytomegalovirus infection in monkeys","volume":"2","author":"CS Nelson","year":"2017","journal-title":"JCI Insight"},{"issue":"2","key":"ppat.1008666.ref028","doi-asserted-by":"crossref","first-page":"e1007968","DOI":"10.1371\/journal.ppat.1007968","article-title":"Intrahost cytomegalovirus population genetics following antibody pretreatment in a monkey model of congenital transmission","volume":"16","author":"D Vera Cruz","year":"2020","journal-title":"PLoS Pathog"},{"issue":"2","key":"ppat.1008666.ref029","doi-asserted-by":"crossref","first-page":"815","DOI":"10.1006\/viro.1993.1323","article-title":"Analysis of the rhesus cytomegalovirus immediate-early gene promoter","volume":"194","author":"DJ Alcendor","year":"1993","journal-title":"Virology"},{"issue":"2","key":"ppat.1008666.ref030","doi-asserted-by":"crossref","first-page":"446","DOI":"10.1086\/514206","article-title":"Neuropathogenesis induced by rhesus cytomegalovirus in fetal rhesus monkeys (Macaca mulatta)","volume":"177","author":"AF Tarantal","year":"1998","journal-title":"J Infect Dis"},{"issue":"8","key":"ppat.1008666.ref031","doi-asserted-by":"crossref","first-page":"3929","DOI":"10.1128\/JVI.02858-15","article-title":"Genetic Stability of Bacterial Artificial Chromosome-Derived Human Cytomegalovirus during Culture In Vitro","volume":"90","author":"I Murrell","year":"2016","journal-title":"J Virol"},{"issue":"Pt 6","key":"ppat.1008666.ref032","doi-asserted-by":"crossref","first-page":"1535","DOI":"10.1099\/vir.0.018994-0","article-title":"Sequential mutations associated with adaptation of human cytomegalovirus to growth in cell culture","volume":"91","author":"DJ Dargan","year":"2010","journal-title":"J Gen Virol"},{"issue":"16","key":"ppat.1008666.ref033","doi-asserted-by":"crossref","first-page":"9310","DOI":"10.1128\/JVI.00162-14","article-title":"Limited dissemination and shedding of the UL128 complex-intact, UL\/b'-defective rhesus cytomegalovirus strain 180.92","volume":"88","author":"BT Assaf","year":"2014","journal-title":"J Virol"},{"issue":"1","key":"ppat.1008666.ref034","doi-asserted-by":"crossref","first-page":"19236","DOI":"10.1038\/s41598-019-55508-w","article-title":"Characterization of a live-attenuated HCMV-based vaccine platform","volume":"9","author":"P Caposio","year":"2019","journal-title":"Sci Rep"},{"issue":"2","key":"ppat.1008666.ref035","doi-asserted-by":"crossref","first-page":"149","DOI":"10.4149\/av_2013_02_149","article-title":"Recombinant herpesviruses as tools for the study of herpesvirus biology","volume":"57","author":"M Kudelova","year":"2013","journal-title":"Acta Virol"},{"issue":"2","key":"ppat.1008666.ref036","doi-asserted-by":"crossref","first-page":"211","DOI":"10.3390\/v4020211","article-title":"Back to BAC: the use of infectious clone technologies for viral mutagenesis","volume":"4","author":"RN Hall","year":"2012","journal-title":"Viruses"},{"issue":"8","key":"ppat.1008666.ref037","doi-asserted-by":"crossref","first-page":"4179","DOI":"10.1128\/JVI.80.8.4179-4182.2006","article-title":"Genomic sequence of rhesus cytomegalovirus 180.92: insights into the coding potential of rhesus cytomegalovirus","volume":"80","author":"P Rivailler","year":"2006","journal-title":"J Virol"},{"issue":"3","key":"ppat.1008666.ref038","doi-asserted-by":"crossref","first-page":"335","DOI":"10.1097\/QAD.0000000000002396","article-title":"Cytomegalovirus-vectored vaccines for HIV and other pathogens","volume":"34","author":"PA Barry","year":"2020","journal-title":"AIDS"},{"issue":"2","key":"ppat.1008666.ref039","doi-asserted-by":"crossref","first-page":"221","DOI":"10.1016\/j.virol.2003.07.003","article-title":"Differential function and expression of the viral inhibitor of caspase 8-induced apoptosis (vICA) and the viral mitochondria-localized inhibitor of apoptosis (vMIA) cell death suppressors conserved in primate and rodent cytomegaloviruses","volume":"316","author":"AL McCormick","year":"2003","journal-title":"Virology"},{"issue":"1\u20132","key":"ppat.1008666.ref040","doi-asserted-by":"crossref","first-page":"208","DOI":"10.1016\/j.virol.2013.08.026","article-title":"Coding potential of UL\/b' from the initial source of rhesus cytomegalovirus Strain 68\u20131","volume":"447","author":"RB Gill","year":"2013","journal-title":"Virology"},{"issue":"11","key":"ppat.1008666.ref041","doi-asserted-by":"crossref","first-page":"e1006014","DOI":"10.1371\/journal.ppat.1006014","article-title":"Cross-Species Rhesus Cytomegalovirus Infection of Cynomolgus Macaques.","volume":"12","author":"BJ Burwitz","year":"2016","journal-title":"PLoS Pathog"},{"issue":"17","key":"ppat.1008666.ref042","doi-asserted-by":"crossref","first-page":"8596","DOI":"10.1128\/JVI.76.17.8596-8608.2002","article-title":"Identification and expression of human cytomegalovirus transcription units coding for two distinct Fcgamma receptor homologs","volume":"76","author":"R Atalay","year":"2002","journal-title":"J Virol"},{"issue":"5","key":"ppat.1008666.ref043","doi-asserted-by":"crossref","first-page":"297","DOI":"10.1038\/nrmicro3018","article-title":"Immune evasion: UL141 keeps HCMV in charge","volume":"11","author":"R. David","year":"2013","journal-title":"Nat Rev Microbiol"},{"key":"ppat.1008666.ref044","doi-asserted-by":"crossref","DOI":"10.7554\/eLife.22206","article-title":"Control of immune ligands by members of a cytomegalovirus gene expansion suppresses natural killer cell activation","volume":"6","author":"CA Fielding","year":"2017","journal-title":"Elife"},{"key":"ppat.1008666.ref045","first-page":"1","volume-title":"Cytomegaloviruses: From Molecular Pathogenesis to Intervention. 1","author":"AJ Davison","year":"2013","edition":"2"},{"issue":"24","key":"ppat.1008666.ref046","doi-asserted-by":"crossref","first-page":"12995","DOI":"10.1128\/JVI.05840-11","article-title":"Genomic sequencing and characterization of cynomolgus macaque cytomegalovirus","volume":"85","author":"AK Marsh","year":"2011","journal-title":"J Virol"},{"key":"ppat.1008666.ref047","doi-asserted-by":"crossref","first-page":"277","DOI":"10.1186\/s12864-016-2588-3","article-title":"A novel strain of cynomolgus macaque cytomegalovirus: implications for host-virus co-evolution","volume":"17","author":"JN Russell","year":"2016","journal-title":"BMC Genomics"},{"issue":"19","key":"ppat.1008666.ref048","doi-asserted-by":"crossref","first-page":"10489","DOI":"10.1128\/JVI.01546-13","article-title":"Impact of sequence variation in the UL128 locus on production of human cytomegalovirus in fibroblast and epithelial cells","volume":"87","author":"I Murrell","year":"2013","journal-title":"J Virol"},{"issue":"9","key":"ppat.1008666.ref049","doi-asserted-by":"crossref","first-page":"3191","DOI":"10.1172\/JCI42955","article-title":"Reconstruction of the complete human cytomegalovirus genome in a BAC reveals RL13 to be a potent inhibitor of replication","volume":"120","author":"RJ Stanton","year":"2010","journal-title":"J Clin Invest"},{"issue":"3","key":"ppat.1008666.ref050","doi-asserted-by":"crossref","DOI":"10.3390\/v11030221","article-title":"Inclusion of Antibodies to Cell Culture Media Preserves the Integrity of Genes Encoding RL13 and the Pentameric Complex Components During Fibroblast Passage of Human Cytomegalovirus","volume":"11","author":"A Ourahmane","year":"2019","journal-title":"Viruses"},{"issue":"12","key":"ppat.1008666.ref051","doi-asserted-by":"crossref","first-page":"5547","DOI":"10.1073\/pnas.89.12.5547","article-title":"Tight control of gene expression in mammalian cells by tetracycline-responsive promoters","volume":"89","author":"M Gossen","year":"1992","journal-title":"Proc Natl Acad Sci U S A"},{"key":"ppat.1008666.ref052","doi-asserted-by":"crossref","DOI":"10.7554\/eLife.18858","article-title":"Rational design of aptazyme riboswitches for efficient control of gene expression in mammalian cells","volume":"5","author":"G Zhong","year":"2016","journal-title":"Elife"},{"issue":"14","key":"ppat.1008666.ref053","doi-asserted-by":"crossref","first-page":"7043","DOI":"10.1073\/pnas.1814850116","article-title":"OR14I1 is a receptor for the human cytomegalovirus pentameric complex and defines viral epithelial cell tropism","volume":"116","author":"X E","year":"2019","journal-title":"Proc Natl Acad Sci U S A"},{"issue":"17","key":"ppat.1008666.ref054","doi-asserted-by":"crossref","first-page":"9680","DOI":"10.1128\/JVI.01167-13","article-title":"Comparative analysis of gO isoforms reveals that strains of human cytomegalovirus differ in the ratio of gH\/gL\/gO and gH\/gL\/UL128-131 in the virion envelope","volume":"87","author":"M Zhou","year":"2013","journal-title":"J Virol"},{"issue":"15","key":"ppat.1008666.ref055","doi-asserted-by":"crossref","DOI":"10.1128\/JVI.00606-18","article-title":"Glycoprotein O (gO) Vary between Strains of Human Cytomegalovirus, Influencing the Assembly of gH\/gL Complexes and Virion Infectivity","volume":"92","author":"L Zhang","year":"2018","journal-title":"J Virol"},{"issue":"16","key":"ppat.1008666.ref056","doi-asserted-by":"crossref","first-page":"10330","DOI":"10.1128\/JVI.79.16.10330-10338.2005","article-title":"Human cytomegalovirus UL131 open reading frame is required for epithelial cell tropism","volume":"79","author":"D Wang","year":"2005","journal-title":"J Virol"},{"issue":"14","key":"ppat.1008666.ref057","doi-asserted-by":"crossref","first-page":"4471","DOI":"10.1073\/pnas.1419875112","article-title":"A viral regulator of glycoprotein complexes contributes to human cytomegalovirus cell tropism","volume":"112","author":"G Li","year":"2015","journal-title":"Proc Natl Acad Sci U S A"},{"issue":"5","key":"ppat.1008666.ref058","doi-asserted-by":"crossref","first-page":"2170","DOI":"10.1128\/JVI.02316-07","article-title":"Functional genetic analysis of rhesus cytomegalovirus: Rh01 is an epithelial cell tropism factor","volume":"82","author":"AE Lilja","year":"2008","journal-title":"J Virol"},{"issue":"8","key":"ppat.1008666.ref059","doi-asserted-by":"crossref","first-page":"e1005868","DOI":"10.1371\/journal.ppat.1005868","article-title":"Natural Killer Cell Evasion Is Essential for Infection by Rhesus Cytomegalovirus","volume":"12","author":"ER Sturgill","year":"2016","journal-title":"PLoS Pathog"},{"issue":"23","key":"ppat.1008666.ref060","doi-asserted-by":"crossref","first-page":"11099","DOI":"10.1128\/JVI.74.23.11099-11107.2000","article-title":"Murine cytomegalovirus containing a mutation at open reading frame M37 is severely attenuated in growth and virulence in vivo","volume":"74","author":"M Lee","year":"2000","journal-title":"J Virol"},{"issue":"13","key":"ppat.1008666.ref061","doi-asserted-by":"crossref","first-page":"E2786","DOI":"10.1073\/pnas.1616829114","article-title":"Mouse cytomegalovirus M36 and M45 death suppressors cooperate to prevent inflammation resulting from antiviral programmed cell death pathways","volume":"114","author":"LP Daley-Bauer","year":"2017","journal-title":"Proc Natl Acad Sci U S A"},{"issue":"5","key":"ppat.1008666.ref062","doi-asserted-by":"crossref","first-page":"1928","DOI":"10.1172\/JCI67420","article-title":"Cytomegalovirus pp65 limits dissemination but is dispensable for persistence","volume":"124","author":"D Malouli","year":"2014","journal-title":"J Clin Invest"},{"issue":"3","key":"ppat.1008666.ref063","doi-asserted-by":"crossref","first-page":"293","DOI":"10.1038\/nm.1935","article-title":"Effector memory T cell responses are associated with protection of rhesus monkeys from mucosal simian immunodeficiency virus challenge","volume":"15","author":"SG Hansen","year":"2009","journal-title":"Nat Med"},{"issue":"49","key":"ppat.1008666.ref064","doi-asserted-by":"crossref","first-page":"E10586","DOI":"10.1073\/pnas.1710522114","article-title":"Transcriptome-wide characterization of human cytomegalovirus in natural infection and experimental latency","volume":"114","author":"S Cheng","year":"2017","journal-title":"Proc Natl Acad Sci U S A"},{"issue":"12","key":"ppat.1008666.ref065","doi-asserted-by":"crossref","first-page":"6620","DOI":"10.1128\/JVI.77.12.6620-6636.2003","article-title":"Complete sequence and genomic analysis of rhesus cytomegalovirus","volume":"77","author":"SG Hansen","year":"2003","journal-title":"J Virol"},{"issue":"4","key":"ppat.1008666.ref066","doi-asserted-by":"crossref","first-page":"831","DOI":"10.1016\/j.cell.2012.05.049","article-title":"Poxviruses deploy genomic accordions to adapt rapidly against host antiviral defenses","volume":"150","author":"NC Elde","year":"2012","journal-title":"Cell"},{"key":"ppat.1008666.ref067","article-title":"Specialization to cell-free or cell-associated spread by BAC-cloned HCMV strains not determined by the UL128-131 and RL13 loci","author":"EP Schultz","year":"2019","journal-title":"bioRxiv"},{"issue":"15","key":"ppat.1008666.ref068","doi-asserted-by":"crossref","first-page":"7673","DOI":"10.1128\/JVI.00578-15","article-title":"High-throughput analysis of human cytomegalovirus genome diversity highlights the widespread occurrence of gene-disrupting mutations and pervasive recombination","volume":"89","author":"S Sijmons","year":"2015","journal-title":"J Virol"},{"issue":"11","key":"ppat.1008666.ref069","doi-asserted-by":"crossref","first-page":"e50166","DOI":"10.1371\/journal.pone.0050166","article-title":"Recombinant human cytomegalovirus (HCMV) RL13 binds human immunoglobulin G Fc","volume":"7","author":"M Cortese","year":"2012","journal-title":"PLoS One"},{"issue":"17","key":"ppat.1008666.ref070","doi-asserted-by":"crossref","first-page":"7715","DOI":"10.1128\/JVI.00320-16","article-title":"Repair of a Mutation Disrupting the Guinea Pig Cytomegalovirus Pentameric Complex Acquired during Fibroblast Passage Restores Pathogenesis in Immune-Suppressed Guinea Pigs and in the Context of Congenital Infection","volume":"90","author":"MA McVoy","year":"2016","journal-title":"J Virol"},{"issue":"1","key":"ppat.1008666.ref071","doi-asserted-by":"crossref","first-page":"173","DOI":"10.1007\/s00705-012-1378-8","article-title":"Recombinant HCMV UL128 expression and functional identification of PBMC-attracting activity in vitro","volume":"158","author":"H Gao","year":"2013","journal-title":"Arch Virol"},{"issue":"3","key":"ppat.1008666.ref072","doi-asserted-by":"crossref","DOI":"10.1128\/mBio.01289-19","article-title":"The Human Cytomegalovirus Chemokine vCXCL-1 Modulates Normal Dissemination Kinetics of Murine Cytomegalovirus In Vivo","volume":"10","author":"JW Jackson","year":"2019","journal-title":"mBio"},{"issue":"1","key":"ppat.1008666.ref073","doi-asserted-by":"crossref","first-page":"49","DOI":"10.1093\/ilar.47.1.49","article-title":"Nonhuman primate models of intrauterine cytomegalovirus infection","volume":"47","author":"PA Barry","year":"2006","journal-title":"ILAR J"},{"key":"ppat.1008666.ref074","doi-asserted-by":"crossref","first-page":"126","DOI":"10.1016\/j.coviro.2017.08.005","article-title":"Rhesus monkeys for a nonhuman primate model of cytomegalovirus infections","volume":"25","author":"HL Itell","year":"2017","journal-title":"Curr Opin Virol"},{"issue":"Supplement_1","key":"ppat.1008666.ref075","doi-asserted-by":"crossref","first-page":"S60","DOI":"10.1093\/infdis\/jiz484","article-title":"Animal Models of Congenital Cytomegalovirus Transmission: Implications for Vaccine Development","volume":"221","author":"HK Roark","year":"2020","journal-title":"J Infect Dis"},{"issue":"2","key":"ppat.1008666.ref076","doi-asserted-by":"crossref","first-page":"135","DOI":"10.1016\/S0166-0934(02)00060-5","article-title":"Replication of rhesus cytomegalovirus in life-expanded rhesus fibroblasts expressing human telomerase","volume":"104","author":"WL Chang","year":"2002","journal-title":"J Virol Methods"},{"issue":"6","key":"ppat.1008666.ref077","doi-asserted-by":"crossref","first-page":"1426","DOI":"10.1099\/jgv.0.000455","article-title":"The susceptibility of primary cultured rhesus macaque kidney epithelial cells to rhesus cytomegalovirus strains","volume":"97","author":"Y Yue","year":"2016","journal-title":"J Gen Virol"},{"key":"ppat.1008666.ref078","doi-asserted-by":"crossref","first-page":"421","DOI":"10.1007\/978-1-60761-652-8_30","article-title":"En passant mutagenesis: a two step markerless red recombination system","volume":"634","author":"BK Tischer","year":"2010","journal-title":"Methods Mol Biol"},{"issue":"4","key":"ppat.1008666.ref079","doi-asserted-by":"crossref","DOI":"10.1128\/JVI.02077-18","article-title":"Identification and Functional Characterization of a Novel Fc Gamma-Binding Glycoprotein in Rhesus Cytomegalovirus","volume":"93","author":"P Kolb","year":"2019","journal-title":"J Virol"},{"issue":"2","key":"ppat.1008666.ref080","doi-asserted-by":"crossref","first-page":"365","DOI":"10.1016\/0022-2836(67)90307-5","article-title":"Selective extraction of polyoma DNA from infected mouse cell cultures","volume":"26","author":"B. Hirt","year":"1967","journal-title":"J Mol Biol"},{"issue":"21","key":"ppat.1008666.ref081","doi-asserted-by":"crossref","first-page":"2947","DOI":"10.1093\/bioinformatics\/btm404","article-title":"Clustal W and Clustal X version 2.0","volume":"23","author":"MA Larkin","year":"2007","journal-title":"Bioinformatics"},{"issue":"13","key":"ppat.1008666.ref082","doi-asserted-by":"crossref","first-page":"1939","DOI":"10.1089\/hum.1998.9.13-1939","article-title":"Tetracycline repressor, tetR, rather than the tetR-mammalian cell transcription factor fusion derivatives, regulates inducible gene expression in mammalian cells","volume":"9","author":"F Yao","year":"1998","journal-title":"Hum Gene Ther"},{"issue":"6","key":"ppat.1008666.ref083","doi-asserted-by":"crossref","first-page":"659","DOI":"10.2144\/000112993","article-title":"Re-engineering adenovirus vector systems to enable high-throughput analyses of gene function","volume":"45","author":"RJ Stanton","year":"2008","journal-title":"Biotechniques"},{"issue":"8","key":"ppat.1008666.ref084","doi-asserted-by":"crossref","first-page":"3646","DOI":"10.1128\/JVI.76.8.3646-3658.2002","article-title":"Decreased frequency of cytomegalovirus (CMV)-specific CD4+ T lymphocytes in simian immunodeficiency virus-infected rhesus macaques: inverse relationship with CMV viremia","volume":"76","author":"A Kaur","year":"2002","journal-title":"J Virol"},{"issue":"7","key":"ppat.1008666.ref085","doi-asserted-by":"crossref","first-page":"1090","DOI":"10.1093\/infdis\/jiu215","article-title":"Postnatal acquisition of primary rhesus cytomegalovirus infection is associated with prolonged virus shedding and impaired CD4+ T lymphocyte function","volume":"210","author":"P Antoine","year":"2014","journal-title":"J Infect Dis"},{"issue":"5\u20136","key":"ppat.1008666.ref086","doi-asserted-by":"crossref","first-page":"303","DOI":"10.1111\/j.1600-0684.2005.00128.x","article-title":"Highly sensitive SIV plasma viral load assay: practical considerations, realistic performance expectations, and application to reverse engineering of vaccines for AIDS","volume":"34","author":"AN Cline","year":"2005","journal-title":"J Med Primatol"},{"issue":"1","key":"ppat.1008666.ref087","doi-asserted-by":"crossref","first-page":"68","DOI":"10.20411\/pai.v1i1.100","article-title":"Defining HIV and SIV Reservoirs in Lymphoid Tissues","volume":"1","author":"C Deleage","year":"2016","journal-title":"Pathog Immun"},{"issue":"8","key":"ppat.1008666.ref088","article-title":"Quantification of T cell Antigen-specific Memory Responses in Rhesus Macaques, Using Cytokine Flow Cytometry (CFC, also Known as ICS and ICCS): Analysis of Flow Data","volume":"4","author":"AW Sylwester","year":"2014","journal-title":"Bio Protoc"},{"issue":"15","key":"ppat.1008666.ref089","doi-asserted-by":"crossref","first-page":"2114","DOI":"10.1093\/bioinformatics\/btu170","article-title":"Trimmomatic: a flexible trimmer for Illumina sequence data","volume":"30","author":"AM Bolger","year":"2014","journal-title":"Bioinformatics"},{"issue":"1","key":"ppat.1008666.ref090","doi-asserted-by":"crossref","first-page":"15","DOI":"10.1093\/bioinformatics\/bts635","article-title":"STAR: ultrafast universal RNA-seq aligner","volume":"29","author":"A Dobin","year":"2013","journal-title":"Bioinformatics"},{"issue":"9","key":"ppat.1008666.ref091","doi-asserted-by":"crossref","first-page":"1297","DOI":"10.1101\/gr.107524.110","article-title":"The Genome Analysis Toolkit: a MapReduce framework for analyzing next-generation DNA sequencing data","volume":"20","author":"A McKenna","year":"2010","journal-title":"Genome Res"},{"key":"ppat.1008666.ref092","doi-asserted-by":"crossref","first-page":"335","DOI":"10.1007\/978-1-4939-3578-9_16","article-title":"Visualizing Genomic Data Using Gviz and Bioconductor","volume":"1418","author":"F Hahne","year":"2016","journal-title":"Methods Mol Biol"},{"issue":"7","key":"ppat.1008666.ref093","doi-asserted-by":"crossref","first-page":"923","DOI":"10.1093\/bioinformatics\/btt656","article-title":"featureCounts: an efficient general purpose program for assigning sequence reads to genomic features","volume":"30","author":"Y Liao","year":"2014","journal-title":"Bioinformatics"},{"issue":"3","key":"ppat.1008666.ref094","doi-asserted-by":"crossref","first-page":"718","DOI":"10.1128\/JVI.26.3.718-723.1978","article-title":"Genetic analysis of a cytomegalovirus-like agent isolated from human brain","volume":"26","author":"ES Huang","year":"1978","journal-title":"J Virol"},{"issue":"1","key":"ppat.1008666.ref095","doi-asserted-by":"crossref","first-page":"93","DOI":"10.1016\/0928-0197(95)00020-9","article-title":"African green monkey origin of the atypical cytopathic 'stealth virus' isolated from a patient with chronic fatigue syndrome","volume":"4","author":"WJ Martin","year":"1995","journal-title":"Clin Diagn Virol"},{"issue":"9","key":"ppat.1008666.ref096","doi-asserted-by":"crossref","first-page":"1723","DOI":"10.1007\/s007050170059","article-title":"Isolation and characterization of an endogenous cytomegalovirus (BaCMV) from baboons","volume":"146","author":"EL Blewett","year":"2001","journal-title":"Arch Virol"},{"issue":"4","key":"ppat.1008666.ref097","doi-asserted-by":"crossref","DOI":"10.1128\/genomeA.00781-15","article-title":"Complete Genome Sequences of Mandrillus leucophaeus and Papio ursinus Cytomegaloviruses","volume":"3","author":"EL Blewett","year":"2015","journal-title":"Genome Announc"},{"issue":"Pt 1","key":"ppat.1008666.ref098","doi-asserted-by":"crossref","first-page":"17","DOI":"10.1099\/vir.0.18606-0","article-title":"The human cytomegalovirus genome revisited: comparison with the chimpanzee cytomegalovirus genome","volume":"84","author":"AJ Davison","year":"2003","journal-title":"J Gen Virol"},{"issue":"3","key":"ppat.1008666.ref099","doi-asserted-by":"crossref","first-page":"835","DOI":"10.3181\/00379727-138-36002","article-title":"Herpes virus aotus: a latent herpesvirus from owl monkeys (Aotus trivirgatus) isolation and characterization","volume":"138","author":"MD Daniel","year":"1971","journal-title":"Proc Soc Exp Biol Med"},{"issue":"3","key":"ppat.1008666.ref100","first-page":"532","article-title":"Isolation and characterization of a cytomegalovirus from the salivary gland of a squirrel monkey (Saimiri sciureus)","volume":"30","author":"SR Rangan","year":"1980","journal-title":"Lab Anim Sci"},{"issue":"16","key":"ppat.1008666.ref101","doi-asserted-by":"crossref","first-page":"7656","DOI":"10.1128\/JVI.74.16.7656-7665.2000","article-title":"Complete DNA sequence of the rat cytomegalovirus genome","volume":"74","author":"C Vink","year":"2000","journal-title":"J Virol"},{"issue":"24","key":"ppat.1008666.ref102","doi-asserted-by":"crossref","first-page":"13838","DOI":"10.1128\/JVI.02614-12","article-title":"Complete genome sequence of the english isolate of rat cytomegalovirus (Murid herpesvirus 8)","volume":"86","author":"J Ettinger","year":"2012","journal-title":"J Virol"},{"issue":"Pt 7","key":"ppat.1008666.ref103","doi-asserted-by":"crossref","first-page":"1873","DOI":"10.1099\/vir.0.000109","article-title":"Rat cytomegalovirus (RCMV) English isolate and a newly identified Berlin isolate share similarities with but are separate as an anciently diverged clade from Mouse CMV and the Maastricht isolate of RCMV","volume":"96","author":"H Geyer","year":"2015","journal-title":"J Gen Virol"},{"issue":"2","key":"ppat.1008666.ref104","doi-asserted-by":"crossref","first-page":"281","DOI":"10.3181\/00379727-96-23455","article-title":"Serial propagation of the guinea pig salivary gland virus in tissue culture","volume":"96","author":"JW Hartley","year":"1957","journal-title":"Proc Soc Exp Biol Med"},{"issue":"3","key":"ppat.1008666.ref105","doi-asserted-by":"crossref","first-page":"435","DOI":"10.3181\/00379727-86-21123","article-title":"Propagation of salivary gland virus of the mouse in tissue cultures","volume":"86","author":"MG Smith","year":"1954","journal-title":"Proc Soc Exp Biol Med"},{"issue":"3","key":"ppat.1008666.ref106","doi-asserted-by":"crossref","first-page":"209","DOI":"10.1002\/ajp.1350170304","article-title":"Blood-protein allele frequencies and phylogenetic relationships in Macaca: A review","volume":"17","author":"J Fooden","year":"1989","journal-title":"Am J Primatol"},{"issue":"2","key":"ppat.1008666.ref107","doi-asserted-by":"crossref","first-page":"182","DOI":"10.1002\/ajp.20337","article-title":"Mitochondrial DNA variation within and among regional populations of longtail macaques (Macaca fascicularis) in relation to other species of the fascicularis group of macaques","volume":"69","author":"DG Smith","year":"2007","journal-title":"Am J Primatol"},{"issue":"2","key":"ppat.1008666.ref108","doi-asserted-by":"crossref","first-page":"242","DOI":"10.1016\/j.gene.2009.05.013","article-title":"Phylogeny of the macaques (Cercopithecidae: Macaca) based on Alu elements","volume":"448","author":"J Li","year":"2009","journal-title":"Gene"},{"key":"ppat.1008666.ref109","first-page":"1","article-title":"Human cytomegalovirus genome","volume":"325","author":"E Murphy","year":"2008","journal-title":"Curr Top Microbiol Immunol"},{"issue":"3","key":"ppat.1008666.ref110","doi-asserted-by":"crossref","first-page":"2397","DOI":"10.3892\/mmr.2017.6944","article-title":"Systematic identification of human housekeeping genes possibly useful as references in gene expression studies","volume":"16","author":"M Caracausi","year":"2017","journal-title":"Mol Med Rep"}],"updated-by":[{"DOI":"10.1371\/journal.ppat.1008666","type":"new_version","label":"New version","source":"publisher","updated":{"date-parts":[[2020,12,8]],"date-time":"2020-12-08T00:00:00Z","timestamp":1607385600000}}],"container-title":["PLOS Pathogens"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/dx.plos.org\/10.1371\/journal.ppat.1008666","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2020,12,8]],"date-time":"2020-12-08T16:54:16Z","timestamp":1607446456000},"score":1,"resource":{"primary":{"URL":"https:\/\/dx.plos.org\/10.1371\/journal.ppat.1008666"}},"subtitle":[],"editor":[{"given":"Robert F.","family":"Kalejta","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2020,11,24]]},"references-count":110,"journal-issue":{"issue":"11","published-online":{"date-parts":[[2020,11,24]]}},"URL":"https:\/\/doi.org\/10.1371\/journal.ppat.1008666","relation":{"has-preprint":[{"id-type":"doi","id":"10.1101\/2020.06.02.129486","asserted-by":"object"}]},"ISSN":["1553-7374"],"issn-type":[{"value":"1553-7374","type":"electronic"}],"subject":[],"published":{"date-parts":[[2020,11,24]]}}}