{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,26]],"date-time":"2026-09-26T22:18:53Z","timestamp":1790461133570,"version":"4.1.0"},"update-to":[{"DOI":"10.1371\/journal.ppat.1008771","type":"new_version","label":"New version","source":"publisher","updated":{"date-parts":[[2021,1,8]],"date-time":"2021-01-08T00:00:00Z","timestamp":1610064000000}}],"reference-count":59,"publisher":"Public Library of Science (PLoS)","issue":"12","license":[{"start":{"date-parts":[[2020,12,28]],"date-time":"2020-12-28T00:00:00Z","timestamp":1609113600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100000060","name":"National Institute of Allergy and Infectious Diseases","doi-asserted-by":"publisher","award":["R01AI134753"],"award-info":[{"award-number":["R01AI134753"]}],"id":[{"id":"10.13039\/100000060","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000060","name":"National Institute of Allergy and Infectious Diseases","doi-asserted-by":"publisher","award":["F31AI136401"],"award-info":[{"award-number":["F31AI136401"]}],"id":[{"id":"10.13039\/100000060","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000057","name":"National Institute of General Medical Sciences","doi-asserted-by":"publisher","award":["T32GM007288"],"award-info":[{"award-number":["T32GM007288"]}],"id":[{"id":"10.13039\/100000057","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Einstein Analytical Imaging Facility","award":["P30CA013330, SIG #1S10OD016214-01A1, SIG #1S10OD019961-01"],"award-info":[{"award-number":["P30CA013330, SIG #1S10OD016214-01A1, SIG #1S10OD019961-01"]}]}],"content-domain":{"domain":["www.plospathogens.org"],"crossmark-restriction":false},"short-container-title":["PLoS Pathog"],"abstract":"<jats:p>\n                    The intracellular parasite\n                    <jats:italic>Toxoplasma gondii<\/jats:italic>\n                    infects a large proportion of humans worldwide and can cause adverse complications in the settings of immune-compromise and pregnancy.\n                    <jats:italic>T<\/jats:italic>\n                    .\n                    <jats:italic>gondii<\/jats:italic>\n                    thrives within many different cell types due in part to its residence within a specialized and heavily modified compartment in which the parasite divides, termed the parasitophorous vacuole. Within this vacuole, numerous proteins optimize intracellular survival following their secretion by the parasite. We investigated the contribution of one of these proteins, TgPPM3C, predicted to contain a PP2C-class serine\/threonine phosphatase domain and previously shown to interact with the protein MYR1, an essential component of a putative vacuolar translocon that mediates effector export into the host cell. Parasites lacking the TgPPM3C gene exhibit a minor growth defect\n                    <jats:italic>in vitro<\/jats:italic>\n                    , are avirulent during acute infection in mice, and form fewer cysts in mouse brain during chronic infection. Phosphoproteomic assessment of TgPPM3C deleted parasite cultures demonstrated alterations in the phosphorylation status of many secreted vacuolar proteins including two exported effector proteins, GRA16 and GRA28, as well as MYR1. Parasites lacking TgPPM3C are defective in GRA16 and GRA28 export, but not in the export of other MYR1-dependant effectors. Phosphomimetic mutation of two GRA16 serine residues results in export defects, suggesting that de-phosphorylation is a critical step in the process of GRA16 export. These findings provide another example of the emerging role of phosphatases in regulating the complex environment of the\n                    <jats:italic>T<\/jats:italic>\n                    .\n                    <jats:italic>gondii<\/jats:italic>\n                    parasitophorous vacuole and influencing the export of specific effector proteins from the vacuolar lumen into the host cell.\n                  <\/jats:p>","DOI":"10.1371\/journal.ppat.1008771","type":"journal-article","created":{"date-parts":[[2020,12,28]],"date-time":"2020-12-28T15:09:16Z","timestamp":1609168156000},"page":"e1008771","update-policy":"https:\/\/doi.org\/10.1371\/journal.ppat.corrections_policy","source":"Crossref","is-referenced-by-count":22,"title":["Toxoplasma gondii PPM3C, a secreted protein phosphatase, affects parasitophorous vacuole effector export"],"prefix":"10.1371","volume":"16","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-0859-2226","authenticated-orcid":true,"given":"Joshua","family":"Mayoral","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8809-3333","authenticated-orcid":true,"given":"Tadakimi","family":"Tomita","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vincent","family":"Tu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0221-354X","authenticated-orcid":true,"given":"Jennifer T.","family":"Aguilan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9073-6641","authenticated-orcid":true,"given":"Simone","family":"Sidoli","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0357-7396","authenticated-orcid":true,"given":"Louis M.","family":"Weiss","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"340","published-online":{"date-parts":[[2020,12,28]]},"reference":[{"issue":"9425","key":"ppat.1008771.ref001","doi-asserted-by":"crossref","first-page":"1965","DOI":"10.1016\/S0140-6736(04)16412-X","article-title":"Toxoplasmosis.","volume":"363","author":"JG Montoya","year":"2004","journal-title":"Lancet"},{"issue":"2","key":"ppat.1008771.ref002","doi-asserted-by":"crossref","first-page":"264","DOI":"10.1128\/CMR.05013-11","article-title":"Epidemiology of and diagnostic strategies for toxoplasmosis","volume":"25","author":"F Robert-Gangneux","year":"2012","journal-title":"Clin Microbiol Rev"},{"issue":"4","key":"ppat.1008771.ref003","doi-asserted-by":"crossref","first-page":"460","DOI":"10.1016\/0014-4894(91)90070-D","article-title":"Toxoplasma gondii: blood and tissue kinetics during acute and chronic infections in mice","volume":"73","author":"F Derouin","year":"1991","journal-title":"Exp Parasitol"},{"issue":"3","key":"ppat.1008771.ref004","doi-asserted-by":"crossref","first-page":"347","DOI":"10.1016\/j.ijpara.2003.11.024","article-title":"Use of molecular and ultrastructural markers to evaluate stage conversion of Toxoplasma gondii in both the intermediate and definitive host","volume":"34","author":"DJ Ferguson","year":"2004","journal-title":"Int J Parasitol"},{"issue":"7","key":"ppat.1008771.ref005","doi-asserted-by":"crossref","first-page":"519","DOI":"10.1016\/j.pt.2017.04.001","article-title":"Brains and Brawn: Toxoplasma Infections of the Central Nervous System and Skeletal Muscle","volume":"33","author":"EA Wohlfert","year":"2017","journal-title":"Trends Parasitol"},{"issue":"2","key":"ppat.1008771.ref006","doi-asserted-by":"crossref","first-page":"60","DOI":"10.1016\/j.pt.2014.12.002","article-title":"Toxoplasma secretory granules: one population or more?","volume":"31","author":"C Mercier","year":"2015","journal-title":"Trends Parasitol"},{"issue":"7","key":"ppat.1008771.ref007","doi-asserted-by":"crossref","first-page":"2397","DOI":"10.1091\/mbc.e02-01-0021","article-title":"Biogenesis of nanotubular network in Toxoplasma parasitophorous vacuole induced by parasite proteins","volume":"13","author":"C Mercier","year":"2002","journal-title":"Mol Biol Cell"},{"issue":"5","key":"ppat.1008771.ref008","doi-asserted-by":"crossref","first-page":"642","DOI":"10.1016\/j.chom.2015.04.003","article-title":"The Toxoplasma Dense Granule Proteins GRA17 and GRA23 Mediate the Movement of Small Molecules between the Host and the Parasitophorous Vacuole.","volume":"17","author":"DA Gold","year":"2015","journal-title":"Cell Host Microbe."},{"key":"ppat.1008771.ref009","article-title":"Characterization of a Toxoplasma effector uncovers an alternative GSK3\/beta-catenin-regulatory pathway of inflammation.","volume":"7","author":"H He","year":"2018","journal-title":"eLife"},{"issue":"3","key":"ppat.1008771.ref010","doi-asserted-by":"crossref","first-page":"615","DOI":"10.1128\/CMR.00005-17","article-title":"Toxoplasma Effectors Targeting Host Signaling and Transcription.","volume":"30","author":"MA Hakimi","year":"2017","journal-title":"Clin Microbiol Rev"},{"issue":"4","key":"ppat.1008771.ref011","doi-asserted-by":"crossref","first-page":"410","DOI":"10.1016\/j.chom.2011.09.004","article-title":"The phosphoproteomes of Plasmodium falciparum and Toxoplasma gondii reveal unusual adaptations within and beyond the parasites' boundaries","volume":"10","author":"M Treeck","year":"2011","journal-title":"Cell Host Microbe"},{"issue":"12","key":"ppat.1008771.ref012","doi-asserted-by":"crossref","first-page":"5853","DOI":"10.1128\/IAI.01667-07","article-title":"The Toxoplasma gondii dense granule protein GRA7 is phosphorylated upon invasion and forms an unexpected association with the rhoptry proteins ROP2 and ROP4","volume":"76","author":"JD Dunn","year":"2008","journal-title":"Infect Immun"},{"issue":"13","key":"ppat.1008771.ref013","doi-asserted-by":"crossref","first-page":"6361","DOI":"10.1073\/pnas.1816161116","article-title":"Divergent kinase regulates membrane ultrastructure of the Toxoplasma parasitophorous vacuole","volume":"116","author":"T Beraki","year":"2019","journal-title":"Proc Natl Acad Sci U S A"},{"issue":"2","key":"ppat.1008771.ref014","doi-asserted-by":"crossref","DOI":"10.1111\/cmi.12651","article-title":"Secreted protein kinases regulate cyst burden during chronic toxoplasmosis","volume":"19","author":"NG Jones","year":"2017","journal-title":"Cell Microbiol"},{"issue":"1","key":"ppat.1008771.ref015","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1111\/mmi.13715","article-title":"The serine\/threonine phosphatases of apicomplexan parasites","volume":"106","author":"C Yang","year":"2017","journal-title":"Molecular microbiology"},{"issue":"12","key":"ppat.1008771.ref016","doi-asserted-by":"crossref","first-page":"1920","DOI":"10.1111\/j.1462-5822.2011.01681.x","article-title":"The organization of the wall filaments and characterization of the matrix structures of Toxoplasma gondii cyst form","volume":"13","author":"L Lemgruber","year":"2011","journal-title":"Cell Microbiol"},{"issue":"12","key":"ppat.1008771.ref017","doi-asserted-by":"crossref","first-page":"1637","DOI":"10.1128\/EC.05182-11","article-title":"Identification of tissue cyst wall components by transcriptome analysis of in vivo and in vitro Toxoplasma gondii bradyzoites","volume":"10","author":"KR Buchholz","year":"2011","journal-title":"Eukaryot Cell"},{"issue":"3","key":"ppat.1008771.ref018","doi-asserted-by":"crossref","first-page":"399","DOI":"10.1128\/EC.00343-12","article-title":"Bradyzoite pseudokinase 1 is crucial for efficient oral infectivity of the Toxoplasma gondii tissue cyst","volume":"12","author":"KR Buchholz","year":"2013","journal-title":"Eukaryot Cell"},{"issue":"12","key":"ppat.1008771.ref019","doi-asserted-by":"crossref","first-page":"e1003823","DOI":"10.1371\/journal.ppat.1003823","article-title":"The Toxoplasma gondii cyst wall protein CST1 is critical for cyst wall integrity and promotes bradyzoite persistence.","volume":"9","author":"T Tomita","year":"2013","journal-title":"PLoS Pathog."},{"issue":"2","key":"ppat.1008771.ref020","article-title":"Enrichment and Proteomic Characterization of the Cyst Wall from In Vitro Toxoplasma gondii Cysts.","volume":"10","author":"V Tu","year":"2019","journal-title":"MBio"},{"issue":"1","key":"ppat.1008771.ref021","doi-asserted-by":"crossref","DOI":"10.1128\/mSphere.00858-19","article-title":"Coimmunoprecipitation with MYR1 Identifies Three Additional Proteins within the Toxoplasma gondii Parasitophorous Vacuole Required for Translocation of Dense Granule Effectors into Host Cells","volume":"5","author":"AM Cygan","year":"2020","journal-title":"mSphere"},{"issue":"1","key":"ppat.1008771.ref022","doi-asserted-by":"crossref","first-page":"e02231","DOI":"10.1128\/mBio.02231-15","article-title":"A Novel Secreted Protein, MYR1, Is Central to Toxoplasma's Manipulation of Host Cells.","volume":"7","author":"M Franco","year":"2016","journal-title":"MBio"},{"issue":"4","key":"ppat.1008771.ref023","doi-asserted-by":"crossref","first-page":"489","DOI":"10.1016\/j.chom.2013.03.002","article-title":"Host cell subversion by Toxoplasma GRA16, an exported dense granule protein that targets the host cell nucleus and alters gene expression","volume":"13","author":"A Bougdour","year":"2013","journal-title":"Cell Host Microbe"},{"issue":"4","key":"ppat.1008771.ref024","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1128\/mBio.00808-16","article-title":"In Vivo Biotinylation of the Toxoplasma Parasitophorous Vacuole Reveals Novel Dense Granule Proteins Important for Parasite Growth and Pathogenesis.","volume":"7","author":"SM Nadipuram","year":"2016","journal-title":"MBio"},{"issue":"3","key":"ppat.1008771.ref025","doi-asserted-by":"crossref","DOI":"10.1128\/mSphere.00402-20","article-title":"Toxoplasma Uses GRA16 To Upregulate Host c-Myc.","volume":"5","author":"MW Panas","year":"2020","journal-title":"mSphere."},{"issue":"9","key":"ppat.1008771.ref026","doi-asserted-by":"crossref","first-page":"1779","DOI":"10.1084\/jem.20160340","article-title":"Toxoplasma gondii TgIST co-opts host chromatin repressors dampening STAT1-dependent gene regulation and IFN-gamma-mediated host defenses","volume":"213","author":"G Gay","year":"2016","journal-title":"J Exp Med"},{"issue":"1","key":"ppat.1008771.ref027","doi-asserted-by":"crossref","first-page":"72","DOI":"10.1016\/j.chom.2016.06.006","article-title":"Toxoplasma Effector Recruits the Mi-2\/NuRD Complex to Repress STAT1 Transcription and Block IFN-gamma-Dependent Gene Expression","volume":"20","author":"P Olias","year":"2016","journal-title":"Cell Host Microbe"},{"key":"ppat.1008771.ref028","article-title":"The Toxoplasma effector TEEGR promotes parasite persistence by modulating NF-kappaB signalling via EZH2","author":"L Braun","year":"2019","journal-title":"Nature microbiology"},{"issue":"2","key":"ppat.1008771.ref029","doi-asserted-by":"crossref","DOI":"10.1128\/mBio.00674-19","article-title":"Toxoplasma Controls Host Cyclin E Expression through the Use of a Novel MYR1-Dependent Effector Protein, HCE1.","volume":"10","author":"MW Panas","year":"2019","journal-title":"MBio"},{"issue":"2","key":"ppat.1008771.ref030","doi-asserted-by":"crossref","DOI":"10.1128\/mBio.00046-20","article-title":"In Vitro Characterization of Protein Effector Export in the Bradyzoite Stage of Toxoplasma gondii.","volume":"11","author":"J Mayoral","year":"2020","journal-title":"mBio."},{"issue":"2","key":"ppat.1008771.ref031","doi-asserted-by":"crossref","first-page":"151","DOI":"10.1111\/cmi.12498","article-title":"The aspartyl protease TgASP5 mediates the export of the Toxoplasma GRA16 and GRA24 effectors into host cells","volume":"18","author":"A Curt-Varesano","year":"2016","journal-title":"Cell Microbiol"},{"issue":"1","key":"ppat.1008771.ref032","doi-asserted-by":"crossref","first-page":"e24360","DOI":"10.4161\/idp.24360","article-title":"The alphabet of intrinsic disorder: I. Act like a Pro: On the abundance and roles of proline residues in intrinsically disordered proteins","volume":"1","author":"FX Theillet","year":"2013","journal-title":"Intrinsically Disord Proteins"},{"issue":"2","key":"ppat.1008771.ref033","doi-asserted-by":"crossref","first-page":"107","DOI":"10.1111\/j.1440-1681.2007.04843.x","article-title":"Functional diversity of mammalian type 2C protein phosphatase isoforms: new tales from an old family.","volume":"35","author":"G Lu","year":"2008","journal-title":"Clin Exp Pharmacol Physiol"},{"issue":"3","key":"ppat.1008771.ref034","doi-asserted-by":"crossref","first-page":"468","DOI":"10.1016\/j.cell.2009.10.006","article-title":"Serine\/threonine phosphatases: mechanism through structure","volume":"139","author":"Y. Shi","year":"2009","journal-title":"Cell"},{"issue":"5","key":"ppat.1008771.ref035","doi-asserted-by":"crossref","first-page":"596","DOI":"10.1038\/nsmb.1795","article-title":"Structures of apicomplexan calcium-dependent protein kinases reveal mechanism of activation by calcium","volume":"17","author":"AK Wernimont","year":"2010","journal-title":"Nat Struct Mol Biol"},{"issue":"13","key":"ppat.1008771.ref036","doi-asserted-by":"crossref","first-page":"3994","DOI":"10.1093\/nar\/gki709","article-title":"Discovery of the principal specific transcription factors of Apicomplexa and their implication for the evolution of the AP2-integrase DNA binding domains","volume":"33","author":"S Balaji","year":"2005","journal-title":"Nucleic acids research"},{"issue":"12","key":"ppat.1008771.ref037","doi-asserted-by":"crossref","first-page":"935","DOI":"10.1016\/j.micinf.2009.06.002","article-title":"A Toxoplasma type 2C serine-threonine phosphatase is involved in parasite growth in the mammalian host cell","volume":"11","author":"G Jan","year":"2009","journal-title":"Microbes Infect"},{"issue":"1","key":"ppat.1008771.ref038","doi-asserted-by":"crossref","first-page":"73","DOI":"10.1128\/EC.00309-06","article-title":"Toxoplasma gondii targets a protein phosphatase 2C to the nuclei of infected host cells","volume":"6","author":"LA Gilbert","year":"2007","journal-title":"Eukaryot Cell"},{"issue":"2","key":"ppat.1008771.ref039","first-page":"114","article-title":"Sequential protein secretion from three distinct organelles of Toxoplasma gondii accompanies invasion of human fibroblasts","volume":"73","author":"VB Carruthers","year":"1997","journal-title":"European journal of cell biology"},{"key":"ppat.1008771.ref040","doi-asserted-by":"crossref","first-page":"69","DOI":"10.1007\/978-1-4939-7737-6_5","article-title":"EuPathDB: The Eukaryotic Pathogen Genomics Database Resource.","volume":"1757","author":"S Warrenfeltz","year":"2018","journal-title":"Methods in molecular biology (Clifton, NJ)."},{"issue":"24","key":"ppat.1008771.ref041","doi-asserted-by":"crossref","first-page":"6798","DOI":"10.1002\/j.1460-2075.1996.tb01071.x","article-title":"Crystal structure of the protein serine\/threonine phosphatase 2C at 2.0 A resolution","volume":"15","author":"AK Das","year":"1996","journal-title":"Embo j"},{"issue":"2\u20133","key":"ppat.1008771.ref042","doi-asserted-by":"crossref","first-page":"121","DOI":"10.1007\/s10969-007-9036-1","article-title":"Structural genomics of protein phosphatases","volume":"8","author":"SC Almo","year":"2007","journal-title":"J Struct Funct Genomics"},{"issue":"4","key":"ppat.1008771.ref043","doi-asserted-by":"crossref","first-page":"1113","DOI":"10.1105\/tpc.15.00102","article-title":"Structural Mechanism Underlying the Specific Recognition between the Arabidopsis State-Transition Phosphatase TAP38\/PPH1 and Phosphorylated Light-Harvesting Complex Protein Lhcb1","volume":"27","author":"X Wei","year":"2015","journal-title":"Plant Cell"},{"issue":"10","key":"ppat.1008771.ref044","doi-asserted-by":"crossref","first-page":"2913","DOI":"10.1111\/pce.13616","article-title":"The flip side of phospho-signalling: Regulation of protein dephosphorylation and the protein phosphatase 2Cs","volume":"42","author":"GB Bhaskara","year":"2019","journal-title":"Plant Cell Environ"},{"issue":"5","key":"ppat.1008771.ref045","doi-asserted-by":"crossref","DOI":"10.1128\/mSphere.00792-20","article-title":"Phosphorylation of Toxoplasma gondii Secreted Proteins during Acute and Chronic Stages of Infection","volume":"5","author":"JC Young","year":"2020","journal-title":"mSphere"},{"issue":"10","key":"ppat.1008771.ref046","doi-asserted-by":"crossref","first-page":"2071","DOI":"10.1084\/jem.20130103","article-title":"A Toxoplasma dense granule protein, GRA24, modulates the early immune response to infection by promoting a direct and sustained host p38 MAPK activation","volume":"210","author":"L Braun","year":"2013","journal-title":"J Exp Med"},{"issue":"1","key":"ppat.1008771.ref047","doi-asserted-by":"crossref","first-page":"e1006828","DOI":"10.1371\/journal.ppat.1006828","article-title":"Identification of a novel protein complex essential for effector translocation across the parasitophorous vacuole membrane of Toxoplasma gondii.","volume":"14","author":"ND Marino","year":"2018","journal-title":"PLoS Pathog."},{"issue":"1","key":"ppat.1008771.ref048","doi-asserted-by":"crossref","DOI":"10.1128\/mSphere.00877-19","article-title":"The Secreted Acid Phosphatase Domain-Containing GRA44 from Toxoplasma gondii Is Required for c-Myc Induction in Infected Cells.","volume":"5","author":"WJ Blakely","year":"2020","journal-title":"mSphere."},{"key":"ppat.1008771.ref049","first-page":"867705","article-title":"A genome-wide loss-of-function screen identifies Toxoplasma gondii genes that determine fitness in interferon gamma-activated murine macrophages.","author":"Y Wang","year":"2019","journal-title":"bioRxiv"},{"issue":"40","key":"ppat.1008771.ref050","doi-asserted-by":"crossref","first-page":"9929","DOI":"10.1073\/pnas.1804177115","article-title":"Relation between single-molecule properties and phase behavior of intrinsically disordered proteins","volume":"115","author":"GL Dignon","year":"2018","journal-title":"Proc Natl Acad Sci U S A"},{"key":"ppat.1008771.ref051","doi-asserted-by":"crossref","first-page":"e04591","DOI":"10.7554\/eLife.04591","article-title":"Regulation of RNA granule dynamics by phosphorylation of serine-rich, intrinsically disordered proteins in C. elegans.","volume":"3","author":"JT Wang","year":"2014","journal-title":"eLife"},{"issue":"12","key":"ppat.1008771.ref052","doi-asserted-by":"crossref","first-page":"2162","DOI":"10.1016\/j.str.2013.09.014","article-title":"Phosphorylation drives a dynamic switch in serine\/arginine-rich proteins","volume":"21","author":"S Xiang","year":"2013","journal-title":"Structure"},{"issue":"9","key":"ppat.1008771.ref053","doi-asserted-by":"crossref","first-page":"1193","DOI":"10.1128\/EC.00297-10","article-title":"Type II Toxoplasma gondii KU80 knockout strains enable functional analysis of genes required for cyst development and latent infection","volume":"10","author":"BA Fox","year":"2011","journal-title":"Eukaryot Cell"},{"key":"ppat.1008771.ref054","doi-asserted-by":"crossref","first-page":"79","DOI":"10.1007\/978-1-4939-6472-7_6","article-title":"Development of CRISPR\/Cas9 for Efficient Genome Editing in Toxoplasma gondii","volume":"1498","author":"B Shen","year":"2017","journal-title":"Methods Mol Biol"},{"issue":"11","key":"ppat.1008771.ref055","doi-asserted-by":"crossref","first-page":"2902","DOI":"10.1093\/nar\/20.11.2902","article-title":"Electroporation in 'intracellular' buffer increases cell survival.","volume":"20","author":"MJ van den Hoff","year":"1992","journal-title":"Nucleic acids research"},{"key":"ppat.1008771.ref056","doi-asserted-by":"crossref","first-page":"8","DOI":"10.1016\/j.ymeth.2015.06.008","article-title":"Characterization of histone post-translational modifications during virus infection using mass spectrometry-based proteomics","volume":"90","author":"K Kulej","year":"2015","journal-title":"Methods"},{"key":"ppat.1008771.ref057","doi-asserted-by":"crossref","first-page":"W281","DOI":"10.1093\/nar\/gks469","article-title":"Seq2Logo: a method for construction and visualization of amino acid binding motifs and sequence profiles including sequence weighting, pseudo counts and two-sided representation of amino acid enrichment and depletion","volume":"40","author":"MC Thomsen","year":"2012","journal-title":"Nucleic acids research"},{"issue":"W1","key":"ppat.1008771.ref058","doi-asserted-by":"crossref","first-page":"W636","DOI":"10.1093\/nar\/gkz268","article-title":"The EMBL-EBI search and sequence analysis tools APIs in 2019","volume":"47","author":"F Madeira","year":"2019","journal-title":"Nucleic acids research"},{"issue":"13","key":"ppat.1008771.ref059","doi-asserted-by":"crossref","first-page":"1605","DOI":"10.1002\/jcc.20084","article-title":"UCSF Chimera\u2014a visualization system for exploratory research and analysis","volume":"25","author":"EF Pettersen","year":"2004","journal-title":"J Comput Chem"}],"updated-by":[{"DOI":"10.1371\/journal.ppat.1008771","type":"new_version","label":"New version","source":"publisher","updated":{"date-parts":[[2021,1,8]],"date-time":"2021-01-08T00:00:00Z","timestamp":1610064000000}}],"container-title":["PLOS Pathogens"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/dx.plos.org\/10.1371\/journal.ppat.1008771","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,1,8]],"date-time":"2021-01-08T16:13:26Z","timestamp":1610122406000},"score":1,"resource":{"primary":{"URL":"https:\/\/dx.plos.org\/10.1371\/journal.ppat.1008771"}},"subtitle":[],"editor":[{"given":"Ira J.","family":"Blader","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2020,12,28]]},"references-count":59,"journal-issue":{"issue":"12","published-online":{"date-parts":[[2020,12,28]]}},"URL":"https:\/\/doi.org\/10.1371\/journal.ppat.1008771","relation":{"has-preprint":[{"id-type":"doi","id":"10.1101\/2020.07.06.189308","asserted-by":"object"}]},"ISSN":["1553-7374"],"issn-type":[{"value":"1553-7374","type":"electronic"}],"subject":[],"published":{"date-parts":[[2020,12,28]]}}}