{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,8]],"date-time":"2026-10-08T12:03:15Z","timestamp":1791460995971,"version":"4.3.4"},"update-to":[{"DOI":"10.1371\/journal.ppat.1008787","type":"new_version","label":"New version","source":"publisher","updated":{"date-parts":[[2021,2,12]],"date-time":"2021-02-12T00:00:00Z","timestamp":1613088000000}}],"reference-count":91,"publisher":"Public Library of Science (PLoS)","issue":"2","license":[{"start":{"date-parts":[[2021,2,2]],"date-time":"2021-02-02T00:00:00Z","timestamp":1612224000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100000060","name":"National Institute of Allergy and Infectious Diseases","doi-asserted-by":"publisher","award":["1R21AI121885-01"],"award-info":[{"award-number":["1R21AI121885-01"]}],"id":[{"id":"10.13039\/100000060","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000057","name":"National Institute of General Medical Sciences","doi-asserted-by":"crossref","award":["1R35GM138316-01"],"award-info":[{"award-number":["1R35GM138316-01"]}],"id":[{"id":"10.13039\/100000057","id-type":"DOI","asserted-by":"crossref"}]},{"name":"University of Connecticut Research Excellence Program"}],"content-domain":{"domain":["www.plospathogens.org"],"crossmark-restriction":false},"short-container-title":["PLoS Pathog"],"abstract":"<jats:p>\n                    <jats:italic>Toxoplasma gondii<\/jats:italic>\n                    is an obligate intracellular parasite that relies on three distinct secretory organelles, the micronemes, rhoptries, and dense granules, for parasite survival and disease pathogenesis. Secretory proteins destined for these organelles are synthesized in the endoplasmic reticulum (ER) and sequentially trafficked through a highly polarized endomembrane network that consists of the Golgi and multiple post-Golgi compartments. Currently, little is known about how the parasite cytoskeleton controls the positioning of the organelles in this pathway, or how vesicular cargo is trafficked between organelles. Here we show that F-actin and an unconventional myosin motor, TgMyoF, control the dynamics and organization of the organelles in the secretory pathway, specifically ER tubule movement, apical positioning of the Golgi and post-Golgi compartments, apical positioning of the rhoptries, and finally, the directed transport of Rab6-positive and Rop1-positive vesicles. Thus, this study identifies TgMyoF and actin as the key cytoskeletal components that organize the endomembrane system in\n                    <jats:italic>T<\/jats:italic>\n                    .\n                    <jats:italic>gondii<\/jats:italic>\n                    .\n                  <\/jats:p>","DOI":"10.1371\/journal.ppat.1008787","type":"journal-article","created":{"date-parts":[[2021,2,2]],"date-time":"2021-02-02T15:47:12Z","timestamp":1612280832000},"page":"e1008787","update-policy":"https:\/\/doi.org\/10.1371\/journal.ppat.corrections_policy","source":"Crossref","is-referenced-by-count":42,"title":["Actin and an unconventional myosin motor, TgMyoF, control the organization and dynamics of the endomembrane network in Toxoplasma gondii"],"prefix":"10.1371","volume":"17","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-4976-818X","authenticated-orcid":true,"given":"Romain","family":"Carmeille","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3130-4766","authenticated-orcid":true,"given":"Porfirio","family":"Schiano Lomoriello","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Parvathi M.","family":"Devarakonda","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9211-7406","authenticated-orcid":true,"given":"Jacob A.","family":"Kellermeier","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7838-3617","authenticated-orcid":true,"given":"Aoife T.","family":"Heaslip","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"340","published-online":{"date-parts":[[2021,2,2]]},"reference":[{"key":"ppat.1008787.ref001","article-title":"The Taxonomy of Sarcocystis (Protozoa, Apicomplexa) Species.","author":"ND Levine","year":"1986","journal-title":"J Parasitol"},{"key":"ppat.1008787.ref002","doi-asserted-by":"crossref","DOI":"10.1201\/9781351076104","article-title":"The protozoan phylum apicomplexa.","author":"ND Levine","year":"2018","journal-title":"The Protozoan Phylum Apicomplexa."},{"key":"ppat.1008787.ref003","doi-asserted-by":"crossref","first-page":"458","DOI":"10.1016\/j.reprotox.2005.10.006","article-title":"Congenital toxoplasmosis-prenatal aspects of Toxoplasma gondii infection.","volume":"21","author":"E Rorman","year":"2006","journal-title":"Reproductive 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parasites.","volume":"7","author":"KM Skillman","year":"2011","journal-title":"PLoS Pathog"},{"issue":"11","key":"ppat.1008787.ref042","doi-asserted-by":"crossref","first-page":"1433","DOI":"10.1111\/j.1600-0854.2006.00484.x","article-title":"Regulation of apicomplexan microfilament dynamics by a minimal set of actin-binding proteins","volume":"7","author":"H Schuler","year":"2006","journal-title":"Traffic"},{"key":"ppat.1008787.ref043","first-page":"248","article-title":"Intracellular Parasite Invasion Strategies","volume":"304","author":"LD Sibley","year":"2004","journal-title":"Science. 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