{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,17]],"date-time":"2026-09-17T17:10:16Z","timestamp":1789665016219,"version":"4.0.1"},"update-to":[{"DOI":"10.1371\/journal.ppat.1008887","type":"new_version","label":"New version","source":"publisher","updated":{"date-parts":[[2020,10,1]],"date-time":"2020-10-01T00:00:00Z","timestamp":1601510400000}}],"reference-count":48,"publisher":"Public Library of Science (PLoS)","issue":"9","license":[{"start":{"date-parts":[[2020,9,21]],"date-time":"2020-09-21T00:00:00Z","timestamp":1600646400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001407","name":"Department of Biotechnology, Ministry of Science and Technology","doi-asserted-by":"publisher","award":["BT\/PR6312"],"award-info":[{"award-number":["BT\/PR6312"]}],"id":[{"id":"10.13039\/501100001407","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001412","name":"Council of Scientific and Industrial Research, India","doi-asserted-by":"publisher","award":["09-512-0181-2012-EMR-I"],"award-info":[{"award-number":["09-512-0181-2012-EMR-I"]}],"id":[{"id":"10.13039\/501100001412","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001409","name":"Department of Science and Technology, Ministry of Science and Technology","doi-asserted-by":"publisher","award":["DST\/INSPIRE\/04\/2014\/002012"],"award-info":[{"award-number":["DST\/INSPIRE\/04\/2014\/002012"]}],"id":[{"id":"10.13039\/501100001409","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["www.plospathogens.org"],"crossmark-restriction":false},"short-container-title":["PLoS Pathog"],"DOI":"10.1371\/journal.ppat.1008887","type":"journal-article","created":{"date-parts":[[2020,9,21]],"date-time":"2020-09-21T18:34:09Z","timestamp":1600713249000},"page":"e1008887","update-policy":"https:\/\/doi.org\/10.1371\/journal.ppat.corrections_policy","source":"Crossref","is-referenced-by-count":19,"title":["Luteolin-mediated Kv1.3 K+ channel inhibition augments BCG vaccine efficacy against tuberculosis by promoting central memory T cell responses in mice"],"prefix":"10.1371","volume":"16","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-5507-1197","authenticated-orcid":true,"given":"Dhiraj Kumar","family":"Singh","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ved Prakash","family":"Dwivedi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shashi Prakash","family":"Singh","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9179-507X","authenticated-orcid":true,"given":"Anjna","family":"Kumari","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Saurabh Kumar","family":"Sharma","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anand","family":"Ranganathan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5275-2309","authenticated-orcid":true,"given":"Luc Van","family":"Kaer","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2713-9558","authenticated-orcid":true,"given":"Gobardhan","family":"Das","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"340","published-online":{"date-parts":[[2020,9,21]]},"reference":[{"key":"ppat.1008887.ref001","first-page":"157","volume-title":"Global tuberculosis report 2017","author":"Organization WH","year":"2017"},{"issue":"9","key":"ppat.1008887.ref002","doi-asserted-by":"crossref","first-page":"698","DOI":"10.1001\/jama.1994.03510330076038","article-title":"Efficacy of BCG vaccine in the prevention of tuberculosis. Meta-analysis of the published literature","volume":"271","author":"GA Colditz","year":"1994","journal-title":"Jama"},{"issue":"2","key":"ppat.1008887.ref003","doi-asserted-by":"crossref","first-page":"831","DOI":"10.4049\/jimmunol.177.2.831","article-title":"Stimulation history dictates memory CD8 T cell phenotype: implications for prime-boost vaccination","volume":"177","author":"D Masopust","year":"2006","journal-title":"Journal of immunology"},{"issue":"7","key":"ppat.1008887.ref004","doi-asserted-by":"crossref","first-page":"1916","DOI":"10.1002\/eji.201040310","article-title":"Modulating numbers and phenotype of CD8+ T cells in secondary immune responses","volume":"40","author":"TC Wirth","year":"2010","journal-title":"European journal of immunology"},{"issue":"1","key":"ppat.1008887.ref005","doi-asserted-by":"crossref","first-page":"128","DOI":"10.1016\/j.immuni.2010.06.014","article-title":"Repetitive antigen stimulation induces stepwise transcriptome diversification but preserves a core signature of memory CD8(+) T cell differentiation","volume":"33","author":"TC Wirth","year":"2010","journal-title":"Immunity"},{"issue":"5528","key":"ppat.1008887.ref006","doi-asserted-by":"crossref","first-page":"248","DOI":"10.1126\/science.1062589","article-title":"Arrested differentiation, the self-renewing memory lymphocyte, and vaccination","volume":"293","author":"DT Fearon","year":"2001","journal-title":"Science"},{"issue":"1","key":"ppat.1008887.ref007","doi-asserted-by":"crossref","first-page":"9","DOI":"10.1038\/ni0102-9","article-title":"Lack of fair play in the T cell response","volume":"3","author":"A Lanzavecchia","year":"2002","journal-title":"Nature immunology"},{"issue":"12","key":"ppat.1008887.ref008","doi-asserted-by":"crossref","first-page":"982","DOI":"10.1038\/nri959","article-title":"Progressive differentiation and selection of the fittest in the immune response","volume":"2","author":"A Lanzavecchia","year":"2002","journal-title":"Nature reviews Immunology"},{"issue":"3","key":"ppat.1008887.ref009","doi-asserted-by":"crossref","first-page":"225","DOI":"10.1038\/ni889","article-title":"Lineage relationship and protective immunity of memory CD8 T cell subsets","volume":"4","author":"EJ Wherry","year":"2003","journal-title":"Nature immunology"},{"key":"ppat.1008887.ref010","doi-asserted-by":"crossref","first-page":"745","DOI":"10.1146\/annurev.immunol.22.012703.104702","article-title":"Central memory and effector memory T cell subsets: function, generation, and maintenance","volume":"22","author":"F Sallusto","year":"2004","journal-title":"Annual review of immunology"},{"issue":"4","key":"ppat.1008887.ref011","doi-asserted-by":"crossref","first-page":"618","DOI":"10.1128\/CVI.00368-09","article-title":"Phenotypic definition of effector and memory T-lymphocyte subsets in mice chronically infected with Mycobacterium tuberculosis","volume":"17","author":"MI Henao-Tamayo","year":"2010","journal-title":"Clinical and vaccine immunology: CVI"},{"issue":"35","key":"ppat.1008887.ref012","doi-asserted-by":"crossref","first-page":"29479","DOI":"10.1074\/jbc.M112.379594","article-title":"Selective inhibition of CCR7(-) effector memory T cell activation by a novel peptide targeting Kv1.3 channel in a rat experimental autoimmune encephalomyelitis model","volume":"287","author":"Z Li","year":"2012","journal-title":"J Biol Chem"},{"issue":"2","key":"ppat.1008887.ref013","doi-asserted-by":"crossref","first-page":"1261","DOI":"10.1074\/jbc.M111.296798","article-title":"Functional blockade of the voltage-gated potassium channel Kv1.3 mediates reversion of T effector to central memory lymphocytes through SMAD3\/p21cip1 signaling","volume":"287","author":"L Hu","year":"2012","journal-title":"J Biol Chem"},{"issue":"12","key":"ppat.1008887.ref014","doi-asserted-by":"crossref","first-page":"5877","DOI":"10.4049\/jimmunol.1103095","article-title":"Kv1.3 deletion biases T cells toward an immunoregulatory phenotype and renders mice resistant to autoimmune encephalomyelitis","volume":"188","author":"AR Gocke","year":"2012","journal-title":"Journal of immunology"},{"issue":"9","key":"ppat.1008887.ref015","doi-asserted-by":"crossref","first-page":"1456","DOI":"10.1093\/infdis\/jiw395","article-title":"Blockade of the Kv1.3 K+ Channel Enhances BCG Vaccine Efficacy by Expanding Central Memory T Lymphocytes","volume":"214","author":"DK Singh","year":"2016","journal-title":"The Journal of infectious diseases"},{"issue":"6","key":"ppat.1008887.ref016","doi-asserted-by":"crossref","first-page":"3042","DOI":"10.1128\/AAC.00260-15","article-title":"Pharmacokinetics and pharmacodynamics of clofazimine in a mouse model of tuberculosis","volume":"59","author":"RV Swanson","year":"2015","journal-title":"Antimicrob Agents Chemother"},{"key":"ppat.1008887.ref017","unstructured":"Lahey TP, Rajadhyaksha V. Inhibition by 3-deoxyflavonoids of t-lymphocyte activation and therapies related thereto. Google Patents; 2005."},{"issue":"6","key":"ppat.1008887.ref018","doi-asserted-by":"crossref","first-page":"2171","DOI":"10.1016\/j.fct.2012.04.011","article-title":"Anti-inflammatory activity of Korean thistle Cirsium maackii and its major flavonoid, luteolin 5-O-glucoside","volume":"50","author":"HA Jung","year":"2012","journal-title":"Food and chemical toxicology: an international journal published for the British Industrial Biological Research Association"},{"issue":"1","key":"ppat.1008887.ref019","doi-asserted-by":"crossref","first-page":"31","DOI":"10.2174\/138955709787001712","article-title":"Distribution and biological activities of the flavonoid luteolin","volume":"9","author":"M Lopez-Lazaro","year":"2009","journal-title":"Mini reviews in medicinal chemistry"},{"issue":"5","key":"ppat.1008887.ref020","doi-asserted-by":"crossref","first-page":"9367","DOI":"10.1021\/acsomega.9b00535","article-title":"Biocatalytic Synthesis of Non-Natural Monoterpene O-Glycosides Exhibiting Superior Antibacterial and Antinematodal Properties","volume":"4","author":"P Bashyal","year":"2019","journal-title":"ACS Omega"},{"issue":"7685","key":"ppat.1008887.ref021","doi-asserted-by":"crossref","first-page":"362","DOI":"10.1038\/nature24633","article-title":"Origin and differentiation of human memory CD8 T cells after vaccination","volume":"552","author":"RS Akondy","year":"2017","journal-title":"Nature"},{"issue":"7685","key":"ppat.1008887.ref022","doi-asserted-by":"crossref","first-page":"404","DOI":"10.1038\/nature25144","article-title":"Effector CD8 T cells dedifferentiate into long-lived memory cells","volume":"552","author":"B Youngblood","year":"2017","journal-title":"Nature"},{"issue":"6","key":"ppat.1008887.ref023","doi-asserted-by":"crossref","first-page":"329","DOI":"10.1016\/j.tube.2010.06.002","article-title":"The Achilles heel of BCG","volume":"90","author":"IM Orme","year":"2010","journal-title":"Tuberculosis (Edinb)"},{"issue":"1","key":"ppat.1008887.ref024","doi-asserted-by":"crossref","first-page":"85","DOI":"10.1016\/j.vaccine.2014.11.013","article-title":"BCG vaccination-induced long-lasting control of Mycobacterium tuberculosis correlates with the accumulation of a novel population of CD4(+)IL-17(+)TNF(+)IL-2(+) T cells","volume":"33","author":"A Cruz","year":"2015","journal-title":"Vaccine"},{"issue":"4","key":"ppat.1008887.ref025","doi-asserted-by":"crossref","first-page":"369","DOI":"10.1038\/ni1449","article-title":"IL-23 and IL-17 in the establishment of protective pulmonary CD4+ T cell responses after vaccination and during Mycobacterium tuberculosis challenge","volume":"8","author":"SA Khader","year":"2007","journal-title":"Nature immunology"},{"issue":"5","key":"ppat.1008887.ref026","doi-asserted-by":"crossref","first-page":"280","DOI":"10.1016\/j.tips.2004.03.010","article-title":"K+ channels as targets for specific immunomodulation","volume":"25","author":"KG Chandy","year":"2004","journal-title":"Trends in pharmacological sciences"},{"issue":"9","key":"ppat.1008887.ref027","doi-asserted-by":"crossref","first-page":"1488","DOI":"10.1093\/infdis\/jix084","article-title":"Reply to Levis and Rendini","volume":"215","author":"DK Singh","year":"2017","journal-title":"The Journal of infectious diseases"},{"issue":"7","key":"ppat.1008887.ref028","doi-asserted-by":"crossref","first-page":"634","DOI":"10.2174\/156800908786241050","article-title":"Luteolin, a flavonoid with potential for cancer prevention and therapy","volume":"8","author":"Y Lin","year":"2008","journal-title":"Current cancer drug targets"},{"issue":"6220","key":"ppat.1008887.ref029","doi-asserted-by":"crossref","first-page":"400","DOI":"10.1126\/science.1260668","article-title":"T cell immunity. Functional heterogeneity of human memory CD4(+) T cell clones primed by pathogens or vaccines","volume":"347","author":"S Becattini","year":"2015","journal-title":"Science"},{"key":"ppat.1008887.ref030","doi-asserted-by":"crossref","first-page":"152","DOI":"10.3389\/fimmu.2014.00152","article-title":"Recombinant BCG: Innovations on an Old Vaccine. Scope of BCG Strains and Strategies to Improve Long-Lasting Memory","volume":"5","author":"AC da Costa","year":"2014","journal-title":"Frontiers in immunology"},{"issue":"48","key":"ppat.1008887.ref031","doi-asserted-by":"crossref","first-page":"33404","DOI":"10.1074\/jbc.M114.600452","article-title":"Simultaneous inhibition of T helper 2 and T regulatory cell differentiation by small molecules enhances Bacillus Calmette-Guerin vaccine efficacy against tuberculosis","volume":"289","author":"D Bhattacharya","year":"2014","journal-title":"J Biol Chem"},{"issue":"1","key":"ppat.1008887.ref032","doi-asserted-by":"crossref","first-page":"288","DOI":"10.1111\/imr.12252","article-title":"Heterogeneity in tuberculosis pathology, microenvironments and therapeutic responses","volume":"264","author":"A Lenaerts","year":"2015","journal-title":"Immunological reviews"},{"key":"ppat.1008887.ref033","doi-asserted-by":"crossref","first-page":"180","DOI":"10.3389\/fimmu.2014.00180","article-title":"Functional Signatures of Human CD4 and CD8 T Cell Responses to Mycobacterium tuberculosis","volume":"5","author":"T Prezzemolo","year":"2014","journal-title":"Frontiers in immunology"},{"issue":"2","key":"ppat.1008887.ref034","doi-asserted-by":"crossref","first-page":"155","DOI":"10.1016\/j.tube.2012.12.002","article-title":"Functional profile of CD4+ and CD8+ T cells in latently infected individuals and patients with active TB","volume":"93","author":"ND Marin","year":"2013","journal-title":"Tuberculosis (Edinb)"},{"issue":"12","key":"ppat.1008887.ref035","doi-asserted-by":"crossref","first-page":"1928","DOI":"10.1093\/infdis\/jiu347","article-title":"Central memory CD4+ T cells are responsible for the recombinant Bacillus Calmette-Guerin DeltaureC::hly vaccine\u2019s superior protection against tuberculosis","volume":"210","author":"A Vogelzang","year":"2014","journal-title":"The Journal of infectious diseases"},{"issue":"9","key":"ppat.1008887.ref036","doi-asserted-by":"crossref","first-page":"5299","DOI":"10.1073\/pnas.95.9.5299","article-title":"Mycobacterium bovis Bacille Calmette-Guerin strains secreting listeriolysin of Listeria monocytogenes","volume":"95","author":"J Hess","year":"1998","journal-title":"Proceedings of the National Academy of Sciences of the United States of America"},{"issue":"9","key":"ppat.1008887.ref037","doi-asserted-by":"crossref","first-page":"2472","DOI":"10.1172\/JCI24617","article-title":"Increased vaccine efficacy against tuberculosis of recombinant Mycobacterium bovis bacille Calmette-Guerin mutants that secrete listeriolysin","volume":"115","author":"L Grode","year":"2005","journal-title":"The Journal of clinical investigation"},{"issue":"2","key":"ppat.1008887.ref038","doi-asserted-by":"crossref","first-page":"172","DOI":"10.1016\/j.smim.2013.04.006","article-title":"Tuberculosis vaccines: time to think about the next generation","volume":"25","author":"SH Kaufmann","year":"2013","journal-title":"Seminars in immunology"},{"issue":"2","key":"ppat.1008887.ref039","doi-asserted-by":"crossref","first-page":"e87340","DOI":"10.1371\/journal.pone.0087340","article-title":"The candidate TB vaccine, MVA85A, induces highly durable Th1 responses","volume":"9","author":"M Tameris","year":"2014","journal-title":"PloS one"},{"issue":"12","key":"ppat.1008887.ref040","doi-asserted-by":"crossref","first-page":"1711","DOI":"10.1084\/jem.194.12.1711","article-title":"Cytokine-driven proliferation and differentiation of human naive, central memory, and effector memory CD4(+) T cells","volume":"194","author":"J Geginat","year":"2001","journal-title":"The Journal of experimental medicine"},{"issue":"6754","key":"ppat.1008887.ref041","doi-asserted-by":"crossref","first-page":"708","DOI":"10.1038\/44385","article-title":"Two subsets of memory T lymphocytes with distinct homing potentials and effector functions","volume":"401","author":"F Sallusto","year":"1999","journal-title":"Nature"},{"issue":"11","key":"ppat.1008887.ref042","doi-asserted-by":"crossref","first-page":"1703","DOI":"10.1172\/JCI16921","article-title":"The voltage-gated Kv1.3 K(+) channel in effector memory T cells as new target for MS","volume":"111","author":"H Wulff","year":"2003","journal-title":"The Journal of clinical investigation"},{"issue":"4","key":"ppat.1008887.ref043","doi-asserted-by":"crossref","first-page":"1369","DOI":"10.1124\/mol.104.008193","article-title":"Targeting effector memory T cells with a selective peptide inhibitor of Kv1.3 channels for therapy of autoimmune diseases","volume":"67","author":"C Beeton","year":"2005","journal-title":"Molecular pharmacology"},{"issue":"24","key":"ppat.1008887.ref044","doi-asserted-by":"crossref","first-page":"13942","DOI":"10.1073\/pnas.241497298","article-title":"Selective blockade of T lymphocyte K(+) channels ameliorates experimental autoimmune encephalomyelitis, a model for multiple sclerosis","volume":"98","author":"C Beeton","year":"2001","journal-title":"Proceedings of the National Academy of Sciences of the United States of America"},{"issue":"46","key":"ppat.1008887.ref045","doi-asserted-by":"crossref","first-page":"17414","DOI":"10.1073\/pnas.0605136103","article-title":"Kv1.3 channels are a therapeutic target for T cell-mediated autoimmune diseases","volume":"103","author":"C Beeton","year":"2006","journal-title":"Proceedings of the National Academy of Sciences of the United States of America"},{"issue":"41","key":"ppat.1008887.ref046","doi-asserted-by":"crossref","first-page":"39443","DOI":"10.1074\/jbc.M304879200","article-title":"Compensatory anion currents in Kv1.3 channel-deficient thymocytes","volume":"278","author":"PA Koni","year":"2003","journal-title":"J Biol Chem"},{"key":"ppat.1008887.ref047","doi-asserted-by":"crossref","first-page":"342","DOI":"10.1016\/j.jep.2018.05.019","article-title":"Anti-inflammatory effects of luteolin: A review of in vitro, in vivo, and in silico studies","volume":"225","author":"N Aziz","year":"2018","journal-title":"J Ethnopharmacol"},{"issue":"4","key":"ppat.1008887.ref048","doi-asserted-by":"crossref","first-page":"1093","DOI":"10.4049\/jimmunol.113.4.1093","article-title":"Separation of T and B lymphocytes by nylon wool columns: evaluation of efficacy by functional assays in vivo","volume":"113","author":"D Trizio","year":"1974","journal-title":"Journal of immunology"}],"updated-by":[{"DOI":"10.1371\/journal.ppat.1009896","type":"correction","label":"Correction","source":"publisher","updated":{"date-parts":[[2021,8,30]],"date-time":"2021-08-30T00:00:00Z","timestamp":1630281600000}},{"DOI":"10.1371\/journal.ppat.1008887","type":"new_version","label":"New version","source":"publisher","updated":{"date-parts":[[2020,10,1]],"date-time":"2020-10-01T00:00:00Z","timestamp":1601510400000}}],"container-title":["PLOS Pathogens"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/dx.plos.org\/10.1371\/journal.ppat.1008887","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,7]],"date-time":"2023-10-07T17:16:00Z","timestamp":1696698960000},"score":1,"resource":{"primary":{"URL":"https:\/\/dx.plos.org\/10.1371\/journal.ppat.1008887"}},"subtitle":[],"editor":[{"given":"David M.","family":"Lewinsohn","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2020,9,21]]},"references-count":48,"journal-issue":{"issue":"9","published-online":{"date-parts":[[2020,9,21]]}},"URL":"https:\/\/doi.org\/10.1371\/journal.ppat.1008887","relation":{"new_version":[{"id-type":"doi","id":"10.1371\/journal.ppat.1008887","asserted-by":"object"}],"correction":[{"id-type":"doi","id":"10.1371\/journal.ppat.1009896","asserted-by":"object"}]},"ISSN":["1553-7374"],"issn-type":[{"value":"1553-7374","type":"electronic"}],"subject":[],"published":{"date-parts":[[2020,9,21]]}}}