{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,7]],"date-time":"2026-04-07T15:28:52Z","timestamp":1775575732612,"version":"3.50.1"},"reference-count":62,"publisher":"Public Library of Science (PLoS)","issue":"11","license":[{"start":{"date-parts":[[2020,11,11]],"date-time":"2020-11-11T00:00:00Z","timestamp":1605052800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["AI126033, AI138949"],"award-info":[{"award-number":["AI126033, AI138949"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100016230","name":"Steven and Alexandra Cohen Foundation","doi-asserted-by":"crossref","id":[{"id":"10.13039\/100016230","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/100000011","name":"Howard Hughes Medical Institute","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100000011","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000011","name":"Howard Hughes Medical Institute","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100000011","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["www.plospathogens.org"],"crossmark-restriction":false},"short-container-title":["PLoS Pathog"],"abstract":"<jats:p>Lyme disease, the most common vector-borne illness in North America, is caused by the spirochete<jats:italic>Borrelia burgdorferi<\/jats:italic>. Infection begins in the skin following a tick bite and can spread to the hearts, joints, nervous system, and other organs. Diverse host responses influence the level of<jats:italic>B<\/jats:italic>.<jats:italic>burgdorferi<\/jats:italic>infection in mice and humans. Using a systems biology approach, we examined potential molecular interactions between human extracellular and secreted proteins and<jats:italic>B<\/jats:italic>.<jats:italic>burgdorferi<\/jats:italic>. A yeast display library expressing 1031 human extracellular proteins was probed against 36 isolates of<jats:italic>B<\/jats:italic>.<jats:italic>burgdorferi sensu lato<\/jats:italic>. We found that human Peptidoglycan Recognition Protein 1 (PGLYRP1) interacted with the vast majority of<jats:italic>B<\/jats:italic>.<jats:italic>burgdorferi<\/jats:italic>isolates. In subsequent experiments, we demonstrated that recombinant PGLYRP1 interacts with purified<jats:italic>B<\/jats:italic>.<jats:italic>burgdorferi<\/jats:italic>peptidoglycan and exhibits borreliacidal activity, suggesting that vertebrate hosts may use PGLYRP1 to identify<jats:italic>B<\/jats:italic>.<jats:italic>burgdorferi<\/jats:italic>. We examined<jats:italic>B<\/jats:italic>.<jats:italic>burgdorferi<\/jats:italic>infection in mice lacking PGLYRP1 and observed an increased spirochete burden in the heart and joints, along with splenomegaly. Mice lacking PGLYRP1 also showed signs of immune dysregulation, including lower serum IgG levels and higher levels of IFN\u03b3, CXCL9, and CXCL10.Taken together, our findings suggest that PGLYRP1 plays a role in the host\u2019s response to<jats:italic>B<\/jats:italic>.<jats:italic>burgdorferi<\/jats:italic>and further demonstrate the utility of expansive yeast display screening in capturing biologically relevant interactions between spirochetes and their hosts.<\/jats:p>","DOI":"10.1371\/journal.ppat.1009030","type":"journal-article","created":{"date-parts":[[2020,11,11]],"date-time":"2020-11-11T18:33:46Z","timestamp":1605119626000},"page":"e1009030","update-policy":"https:\/\/doi.org\/10.1371\/journal.ppat.corrections_policy","source":"Crossref","is-referenced-by-count":27,"title":["A human secretome library screen reveals a role for Peptidoglycan Recognition Protein 1 in Lyme borreliosis"],"prefix":"10.1371","volume":"16","author":[{"given":"Akash","family":"Gupta","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0575-6205","authenticated-orcid":true,"given":"Gunjan","family":"Arora","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2442-3676","authenticated-orcid":true,"given":"Connor 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W.","family":"Hovius","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2153-738X","authenticated-orcid":true,"given":"Carmen J.","family":"Booth","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Christine","family":"Jacobs-Wagner","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7262-9455","authenticated-orcid":true,"given":"Noah W.","family":"Palm","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3699-2446","authenticated-orcid":true,"given":"Aaron 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Immun."},{"issue":"1","key":"ppat.1009030.ref021","doi-asserted-by":"crossref","first-page":"129455","DOI":"10.1016\/j.bbagen.2019.129455","article-title":"Characterization of 5'-methylthioadenosine\/S-adenosylhomocysteine nucleosidases from Borrelia burgdorferi: Antibiotic targets for Lyme disease","author":"KA Cornell","year":"2020","journal-title":"Biochim Biophys Acta Gen Subj"},{"issue":"2","key":"ppat.1009030.ref022","doi-asserted-by":"crossref","first-page":"205","DOI":"10.1093\/infdis\/jiw151","article-title":"Pre-exposure Prophylaxis With OspA-Specific Human Monoclonal Antibodies Protects Mice Against Tick Transmission of Lyme Disease Spirochetes","volume":"214","author":"Y Wang","year":"2016","journal-title":"J Infect Dis"},{"issue":"4","key":"ppat.1009030.ref023","doi-asserted-by":"crossref","DOI":"10.1128\/IAI.00917-19","article-title":"Broadly Protective Multivalent OspA Vaccine against Lyme Borreliosis, Developed Based on Surface Shaping of the C-Terminal 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Das","year":"1997","journal-title":"J Clin Invest"},{"issue":"1","key":"ppat.1009030.ref033","doi-asserted-by":"crossref","first-page":"382","DOI":"10.1128\/JCM.38.1.382-388.2000","article-title":"Temporal changes in outer surface proteins A and C of the lyme disease-associated spirochete, Borrelia burgdorferi, during the chain of infection in ticks and mice","volume":"38","author":"TG Schwan","year":"2000","journal-title":"J Clin Microbiol"},{"issue":"6","key":"ppat.1009030.ref034","doi-asserted-by":"crossref","first-page":"3618","DOI":"10.1128\/IAI.69.6.3618-3627.2001","article-title":"Regulation of OspE-related, OspF-related, and Elp lipoproteins of Borrelia burgdorferi strain 297 by mammalian host-specific signals","volume":"69","author":"PS Hefty","year":"2001","journal-title":"Infect Immun"},{"issue":"1","key":"ppat.1009030.ref035","doi-asserted-by":"crossref","first-page":"89","DOI":"10.1016\/0006-291X(90)91734-A","article-title":"Isolation, preliminary chemical 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Sharma","year":"2011","journal-title":"J Biol Chem"},{"issue":"4","key":"ppat.1009030.ref041","doi-asserted-by":"crossref","first-page":"264","DOI":"10.1038\/nrmicro1620","article-title":"Peptidoglycan recognition proteins: pleiotropic sensors and effectors of antimicrobial defences","volume":"5","author":"J Royet","year":"2007","journal-title":"Nat Rev Microbiol"},{"issue":"49","key":"ppat.1009030.ref042","doi-asserted-by":"crossref","first-page":"17168","DOI":"10.1073\/pnas.0407856101","article-title":"Structural basis for peptidoglycan binding by peptidoglycan recognition proteins","volume":"101","author":"R Guan","year":"2004","journal-title":"Proc Natl Acad Sci U S A"},{"issue":"8","key":"ppat.1009030.ref043","doi-asserted-by":"crossref","first-page":"232","DOI":"10.1186\/gb-2006-7-8-232","article-title":"The peptidoglycan recognition proteins (PGRPs)","volume":"7","author":"R Dziarski","year":"2006","journal-title":"Genome 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