{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,22]],"date-time":"2026-10-22T07:37:21Z","timestamp":1792654641538,"version":"4.5.0"},"update-to":[{"DOI":"10.1371\/journal.ppat.1009072","type":"new_version","label":"New version","source":"publisher","updated":{"date-parts":[[2021,3,2]],"date-time":"2021-03-02T00:00:00Z","timestamp":1614643200000}}],"reference-count":88,"publisher":"Public Library of Science (PLoS)","issue":"2","license":[{"start":{"date-parts":[[2021,2,18]],"date-time":"2021-02-18T00:00:00Z","timestamp":1613606400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/publicdomain\/zero\/1.0\/"}],"funder":[{"DOI":"10.13039\/100007254","name":"Creighton University","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100007254","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000060","name":"National Institute of Allergy and Infectious Diseases","doi-asserted-by":"publisher","award":["AI000905"],"award-info":[{"award-number":["AI000905"]}],"id":[{"id":"10.13039\/100000060","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["www.plospathogens.org"],"crossmark-restriction":false},"short-container-title":["PLoS Pathog"],"abstract":"<jats:p>\n                    Throughout its enzootic cycle, the Lyme disease spirochete\n                    <jats:italic>Borreliella<\/jats:italic>\n                    (\n                    <jats:italic>Borrelia<\/jats:italic>\n                    )\n                    <jats:italic>burgdorferi<\/jats:italic>\n                    , senses and responds to changes in its environment using a small repertoire of transcription factors that coordinate the expression of genes required for infection of\n                    <jats:italic>Ixodes<\/jats:italic>\n                    ticks and various mammalian hosts. Among these transcription factors, the DnaK suppressor protein (DksA) plays a pivotal role in regulating gene expression in\n                    <jats:italic>B<\/jats:italic>\n                    .\n                    <jats:italic>burgdorferi<\/jats:italic>\n                    during periods of nutrient limitation and is required for mammalian infectivity. In many pathogenic bacteria, the gene regulatory activity of DksA, along with the alarmone guanosine penta- and tetra-phosphate ((p)ppGpp), coordinate the stringent response to various environmental stresses, including nutrient limitation. In this study, we sought to characterize the role of DksA in regulating the transcriptional activity of RNA polymerase and its role in the regulation of RpoS-dependent gene expression required for\n                    <jats:italic>B<\/jats:italic>\n                    .\n                    <jats:italic>burgdorferi<\/jats:italic>\n                    infectivity. Using\n                    <jats:italic>in vitro<\/jats:italic>\n                    transcription assays, we observed recombinant DksA inhibits RpoD-dependent transcription by\n                    <jats:italic>B<\/jats:italic>\n                    .\n                    <jats:italic>burgdorferi<\/jats:italic>\n                    RNA polymerase independent of ppGpp. Additionally, we determined the pH-inducible expression of RpoS-dependent genes relies on DksA, but this relationship is independent of (p)ppGpp produced by Rel\n                    <jats:sub>bbu<\/jats:sub>\n                    . Subsequent transcriptomic and western blot assays indicate DksA regulates the expression of BBD18, a protein previously implicated in the post-transcriptional regulation of RpoS. Moreover, we observed DksA was required for infection of mice following intraperitoneal inoculation or for transmission of\n                    <jats:italic>B<\/jats:italic>\n                    .\n                    <jats:italic>burgdorferi<\/jats:italic>\n                    by\n                    <jats:italic>Ixodes scapularis<\/jats:italic>\n                    nymphs. Together, these data suggest DksA plays a central role in coordinating transcriptional responses in\n                    <jats:italic>B<\/jats:italic>\n                    .\n                    <jats:italic>burgdorferi<\/jats:italic>\n                    required for infectivity through DksA\u2019s interactions with RNA polymerase and post-transcriptional control of RpoS.\n                  <\/jats:p>","DOI":"10.1371\/journal.ppat.1009072","type":"journal-article","created":{"date-parts":[[2021,2,18]],"date-time":"2021-02-18T15:15:24Z","timestamp":1613661324000},"page":"e1009072","update-policy":"https:\/\/doi.org\/10.1371\/journal.ppat.corrections_policy","source":"Crossref","is-referenced-by-count":14,"title":["DksA-dependent regulation of RpoS contributes to Borrelia burgdorferi tick-borne transmission and mammalian infectivity"],"prefix":"10.1371","volume":"17","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-9322-6751","authenticated-orcid":true,"given":"William K.","family":"Boyle","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5112-5095","authenticated-orcid":true,"given":"Crystal L.","family":"Richards","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Daniel P.","family":"Dulebohn","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2119-5372","authenticated-orcid":true,"given":"Amanda K.","family":"Zalud","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6064-6012","authenticated-orcid":true,"given":"Jeff A.","family":"Shaw","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5089-5186","authenticated-orcid":true,"given":"S\u00e1ndor","family":"Lovas","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1063-7131","authenticated-orcid":true,"given":"Frank C.","family":"Gherardini","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4154-929X","authenticated-orcid":true,"given":"Travis J.","family":"Bourret","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"340","published-online":{"date-parts":[[2021,2,18]]},"reference":[{"issue":"3","key":"ppat.1009072.ref001","first-page":"1","article-title":"Surveillance for Lyme disease\u2014United States, 1992\u20131998","volume":"49","author":"KA Orloski","year":"2000","journal-title":"MMWR CDC Surveill Summ"},{"key":"ppat.1009072.ref002","unstructured":"Centers for Disease Control. 2018. Lyme Disease. 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