{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,28]],"date-time":"2026-09-28T21:54:07Z","timestamp":1790632447201,"version":"4.1.0"},"update-to":[{"DOI":"10.1371\/journal.ppat.1009749","type":"new_version","label":"New version","source":"publisher","updated":{"date-parts":[[2021,8,10]],"date-time":"2021-08-10T00:00:00Z","timestamp":1628553600000}}],"reference-count":51,"publisher":"Public Library of Science (PLoS)","issue":"7","license":[{"start":{"date-parts":[[2021,7,29]],"date-time":"2021-07-29T00:00:00Z","timestamp":1627516800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001665","name":"Agence Nationale de la Recherche","doi-asserted-by":"publisher","award":["ANR-10-LABX- 77-01"],"award-info":[{"award-number":["ANR-10-LABX- 77-01"]}],"id":[{"id":"10.13039\/501100001665","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["NIH R01AI146420"],"award-info":[{"award-number":["NIH R01AI146420"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100007492","name":"Baylor University","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100007492","id-type":"DOI","asserted-by":"publisher"}]},{"name":"agence nationale de recherche","award":["ANR-10-LABX-77"],"award-info":[{"award-number":["ANR-10-LABX-77"]}]}],"content-domain":{"domain":["www.plospathogens.org"],"crossmark-restriction":false},"short-container-title":["PLoS Pathog"],"abstract":"<jats:p>The main avenue for the development of an HIV-1 vaccine remains the induction of protective antibodies. A rationale approach is to target antigen to specific receptors on dendritic cells (DC) via fused monoclonal antibodies (mAb). In mouse and non-human primate models, targeting of skin Langerhans cells (LC) with anti-Langerin mAbs fused with HIV-1 Gag antigen drives antigen-specific humoral responses. The development of these immunization strategies in humans requires a better understanding of early immune events driven by human LC. We therefore produced anti-Langerin mAbs fused with the HIV-1 gp140z Envelope (\u03b1LC.Env). First, we show that primary skin human LC and <jats:italic>in vitro<\/jats:italic> differentiated LC induce differentiation and expansion of na\u00efve CD4<jats:sup>+<\/jats:sup> T cells into T follicular helper (Tfh) cells. Second, when human LC are pre-treated with \u03b1LC.Env, differentiated Tfh cells significantly promote the production of specific IgG by B cells. Strikingly, HIV-Env-specific Ig are secreted by HIV-specific memory B cells. Consistently, we found that receptors and cytokines involved in Tfh differentiation and B cell functions are upregulated by LC during their maturation and after targeting Langerin. Finally, we show that subcutaneous immunization of mice by \u03b1LC.Env induces germinal center (GC) reaction in draining lymph nodes with higher numbers of Tfh cells, Env-specific B cells, as well as specific IgG serum levels compared to mice immunized with the non-targeting Env antigen. Altogether, we provide evidence that human LC properly targeted may be licensed to efficiently induce Tfh cell and B cell responses in GC.<\/jats:p>","DOI":"10.1371\/journal.ppat.1009749","type":"journal-article","created":{"date-parts":[[2021,7,29]],"date-time":"2021-07-29T18:48:45Z","timestamp":1627584525000},"page":"e1009749","update-policy":"https:\/\/doi.org\/10.1371\/journal.ppat.corrections_policy","source":"Crossref","is-referenced-by-count":18,"title":["Targeting human langerin promotes HIV-1 specific humoral immune responses"],"prefix":"10.1371","volume":"17","author":[{"given":"J\u00e9r\u00f4me","family":"Kervevan","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0186-8816","authenticated-orcid":true,"given":"Aur\u00e9lie","family":"Bouteau","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Juliane 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Donnelly","year":"2011","journal-title":"Vaccine"},{"key":"ppat.1009749.ref044","doi-asserted-by":"crossref","first-page":"4842","DOI":"10.4049\/jimmunol.0901695","article-title":"Langerhans Cells Prime IL-17\u2013Producing T Cells and Dampen Genital Cytotoxic Responses following Mucosal Immunization","volume":"184","author":"C Hervouet","year":"2010","journal-title":"J Immunol"},{"key":"ppat.1009749.ref045","doi-asserted-by":"crossref","first-page":"35","DOI":"10.1016\/j.micpath.2012.11.010","article-title":"Dendritic cells and vaccine design for sexually-transmitted diseases","volume":"58","author":"D Duluc","year":"2013","journal-title":"Microb Pathog"},{"key":"ppat.1009749.ref046","doi-asserted-by":"crossref","first-page":"1","DOI":"10.15252\/embr.201949351","article-title":"Anti- HIV -1 antibodies trigger non-lytic complement deposition on infected cells","volume":"21","author":"J Dufloo","year":"2020","journal-title":"EMBO 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