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Wadsworth Center","doi-asserted-by":"publisher","award":["Start-up grant"],"award-info":[{"award-number":["Start-up grant"]}],"id":[{"id":"10.13039\/100004857","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100004857","name":"New York State Department of Health - Wadsworth Center","doi-asserted-by":"publisher","award":["Start-up grant"],"award-info":[{"award-number":["Start-up grant"]}],"id":[{"id":"10.13039\/100004857","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001824","name":"Czech Science Foundation","doi-asserted-by":"crossref","award":["17-21244S"],"award-info":[{"award-number":["17-21244S"]}],"id":[{"id":"10.13039\/501100001824","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100003072","name":"LOEWE Zentrum AdRIA","doi-asserted-by":"publisher","award":["DRUID Novel Drug Targets against Poverty-Related and Neglected Tropical Infectious Diseases, project C3"],"award-info":[{"award-number":["DRUID Novel Drug Targets against Poverty-Related and Neglected Tropical Infectious Diseases, project C3"]}],"id":[{"id":"10.13039\/501100003072","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["www.plospathogens.org"],"crossmark-restriction":false},"short-container-title":["PLoS Pathog"],"abstract":"<jats:p>\n                    Pathogens possess the ability to adapt and survive in some host species but not in others\u2013an ecological trait known as host tropism. Transmitted through ticks and carried mainly by mammals and birds, the Lyme disease (LD) bacterium is a well-suited model to study such tropism. Three main causative agents of LD,\n                    <jats:italic>Borrelia burgdorferi<\/jats:italic>\n                    ,\n                    <jats:italic>B<\/jats:italic>\n                    .\n                    <jats:italic>afzelii<\/jats:italic>\n                    , and\n                    <jats:italic>B<\/jats:italic>\n                    .\n                    <jats:italic>garinii<\/jats:italic>\n                    , vary in host ranges through mechanisms eluding characterization. By feeding ticks infected with different\n                    <jats:italic>Borrelia<\/jats:italic>\n                    species, utilizing feeding chambers and live mice and quail, we found species-level differences in bacterial transmission. These differences localize on the tick blood meal, and specifically complement, a defense in vertebrate blood, and a polymorphic bacterial protein, CspA, which inactivates complement by binding to a host complement inhibitor, Factor H (FH). CspA selectively confers bacterial transmission to vertebrates that produce FH capable of allele-specific recognition. CspA is the only member of the Pfam54 gene family to exhibit host-specific FH-binding. Phylogenetic analyses revealed convergent evolution as the driver of such uniqueness, and that FH-binding likely emerged during the last glacial maximum. Our results identify a determinant of host tropism in Lyme disease infection, thus defining an evolutionary mechanism that shapes host-pathogen associations.\n                  <\/jats:p>","DOI":"10.1371\/journal.ppat.1009801","type":"journal-article","created":{"date-parts":[[2021,7,29]],"date-time":"2021-07-29T14:48:53Z","timestamp":1627570133000},"page":"e1009801","update-policy":"https:\/\/doi.org\/10.1371\/journal.ppat.corrections_policy","source":"Crossref","is-referenced-by-count":27,"title":["Host tropism determination by convergent evolution of immunological evasion in the Lyme disease system"],"prefix":"10.1371","volume":"17","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-5949-9932","authenticated-orcid":true,"given":"Thomas M.","family":"Hart","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0703-9250","authenticated-orcid":true,"given":"Alan P.","family":"Dupuis","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8084-7556","authenticated-orcid":true,"given":"Danielle M.","family":"Tufts","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1348-1734","authenticated-orcid":true,"given":"Anna M.","family":"Blom","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Simon R.","family":"Starkey","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6932-0940","authenticated-orcid":true,"given":"Ryan O. 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