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However, invasive and allergic aspergillosis can develop in immunocompromised and atopic individuals, respectively. Previously, we demonstrated that mouse lung eosinophils produce IL-17 in response to stimulation by live conidia and antigens of <jats:italic>A<\/jats:italic>. <jats:italic>fumigatus<\/jats:italic>. Here, we utilized murine models of allergic and acute pulmonary aspergillosis to determine the association of IL-23, IL-23R and ROR\u03b3t with eosinophil IL-17 expression. Following <jats:italic>A<\/jats:italic>. <jats:italic>fumigatus<\/jats:italic> stimulation, a population of lung eosinophils expressed ROR\u03b3t, the master transcription factor for IL-17 regulation. Eosinophil ROR\u03b3t expression was demonstrated by flow cytometry, confocal microscopy, western blotting and an mCherry reporter mouse. Both nuclear and cytoplasmic localization of ROR\u03b3t in eosinophils were observed, although the former predominated. A population of lung eosinophils also expressed IL-23R. While expression of IL-23R was positively correlated with expression of ROR\u03b3t, expression of ROR\u03b3t and IL-17 was similar when comparing lung eosinophils from <jats:italic>A<\/jats:italic>. <jats:italic>fumigatus<\/jats:italic>-challenged wild-type and IL-23p19<jats:sup>-\/-<\/jats:sup> mice. Thus, in allergic and acute models of pulmonary aspergillosis, lung eosinophils express IL-17, ROR\u03b3t and IL-23R. However, IL-23 is dispensable for production of IL-17 and ROR\u03b3t.<\/jats:p>","DOI":"10.1371\/journal.ppat.1009891","type":"journal-article","created":{"date-parts":[[2021,8,31]],"date-time":"2021-08-31T18:16:57Z","timestamp":1630433817000},"page":"e1009891","update-policy":"https:\/\/doi.org\/10.1371\/journal.ppat.corrections_policy","source":"Crossref","is-referenced-by-count":17,"title":["Lung eosinophils elicited during allergic and acute aspergillosis express ROR\u03b3t and IL-23R but do not require IL-23 for IL-17 production"],"prefix":"10.1371","volume":"17","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-7502-8196","authenticated-orcid":true,"given":"Bhawna","family":"Yadav","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5749-3112","authenticated-orcid":true,"given":"Charles 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