{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,25]],"date-time":"2026-09-25T13:14:05Z","timestamp":1790342045614,"version":"4.1.0"},"update-to":[{"DOI":"10.1371\/journal.ppat.1009977","type":"new_version","label":"New version","source":"publisher","updated":{"date-parts":[[2022,3,4]],"date-time":"2022-03-04T00:00:00Z","timestamp":1646352000000}}],"reference-count":72,"publisher":"Public Library of Science (PLoS)","issue":"2","license":[{"start":{"date-parts":[[2022,2,22]],"date-time":"2022-02-22T00:00:00Z","timestamp":1645488000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Melbourne Research Scholarship"},{"name":"Deakin University Postgraduate Research Scholarship"},{"name":"NHMRC Senior Research Fellowship","award":["1136300"],"award-info":[{"award-number":["1136300"]}]},{"name":"Ellen Corin Fellowship"},{"name":"Nora Baart Foundation"},{"name":"Victorian Operational Infrastructure Support Program"},{"DOI":"10.13039\/501100000925","name":"National Health and Medical Research Council","doi-asserted-by":"publisher","award":["1092789"],"award-info":[{"award-number":["1092789"]}],"id":[{"id":"10.13039\/501100000925","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100000925","name":"National Health and Medical Research Council","doi-asserted-by":"publisher","award":["1128198"],"award-info":[{"award-number":["1128198"]}],"id":[{"id":"10.13039\/501100000925","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100000925","name":"National Health and Medical Research Council","doi-asserted-by":"publisher","award":["1197805"],"award-info":[{"award-number":["1197805"]}],"id":[{"id":"10.13039\/501100000925","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["www.plospathogens.org"],"crossmark-restriction":false},"short-container-title":["PLoS Pathog"],"abstract":"<jats:p>\n                    <jats:italic>Plasmodium falciparum<\/jats:italic>\n                    exports ~10% of its proteome into its host erythrocyte to modify the host cell\u2019s physiology. The\n                    <jats:italic>Plasmodium<\/jats:italic>\n                    export element (PEXEL) motif contained within the N-terminus of most exported proteins directs the trafficking of those proteins into the erythrocyte. To reach the host cell, the PEXEL motif of exported proteins is processed by the endoplasmic reticulum (ER) resident aspartyl protease plasmepsin V. Then, following secretion into the parasite-encasing parasitophorous vacuole, the mature exported protein must be unfolded and translocated across the parasitophorous vacuole membrane by the\n                    <jats:italic>Plasmodium<\/jats:italic>\n                    translocon of exported proteins (PTEX). PTEX is a protein-conducting channel consisting of the pore-forming protein EXP2, the protein unfoldase HSP101, and structural component PTEX150. The mechanism of how exported proteins are specifically trafficked from the parasite\u2019s ER following PEXEL cleavage to PTEX complexes on the parasitophorous vacuole membrane is currently not understood. Here, we present evidence that EXP2 and PTEX150 form a stable subcomplex that facilitates HSP101 docking. We also demonstrate that HSP101 localises both within the parasitophorous vacuole and within the parasite\u2019s ER throughout the ring and trophozoite stage of the parasite, coinciding with the timeframe of protein export. Interestingly, we found that HSP101 can form specific interactions with model PEXEL proteins in the parasite\u2019s ER, irrespective of their PEXEL processing status. Collectively, our data suggest that HSP101 recognises and chaperones PEXEL proteins from the ER to the parasitophorous vacuole and given HSP101\u2019s specificity for the EXP2-PTEX150 subcomplex, this provides a mechanism for how exported proteins are specifically targeted to PTEX for translocation into the erythrocyte.\n                  <\/jats:p>","DOI":"10.1371\/journal.ppat.1009977","type":"journal-article","created":{"date-parts":[[2022,2,22]],"date-time":"2022-02-22T13:39:33Z","timestamp":1645537173000},"page":"e1009977","update-policy":"https:\/\/doi.org\/10.1371\/journal.ppat.corrections_policy","source":"Crossref","is-referenced-by-count":35,"title":["A revised mechanism for how Plasmodium falciparum recruits and exports proteins into its erythrocytic host cell"],"prefix":"10.1371","volume":"18","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-8983-9790","authenticated-orcid":true,"given":"Mikha","family":"Gabriela","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7008-3399","authenticated-orcid":true,"given":"Kathryn M.","family":"Matthews","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7678-3863","authenticated-orcid":true,"given":"Cas","family":"Boshoven","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6777-4076","authenticated-orcid":true,"given":"Betty","family":"Kouskousis","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0618-4731","authenticated-orcid":true,"given":"Thorey K.","family":"Jonsdottir","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hayley E.","family":"Bullen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Joyanta","family":"Modak","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5656-1569","authenticated-orcid":true,"given":"David L.","family":"Steer","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9117-1048","authenticated-orcid":true,"given":"Brad E.","family":"Sleebs","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0480-5557","authenticated-orcid":true,"given":"Brendan S.","family":"Crabb","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5810-8063","authenticated-orcid":true,"given":"Tania F. de","family":"Koning-Ward","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8778-6795","authenticated-orcid":true,"given":"Paul R.","family":"Gilson","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"340","published-online":{"date-parts":[[2022,2,22]]},"reference":[{"key":"ppat.1009977.ref001","unstructured":"World Health Organization. World malaria report 2020: 20 years of global progress and challenges. 2020."},{"issue":"3","key":"ppat.1009977.ref002","doi-asserted-by":"crossref","first-page":"610","DOI":"10.1016\/j.cell.2016.07.055","article-title":"Malaria: biology and disease","volume":"167","author":"AF Cowman","year":"2016","journal-title":"Cell"},{"issue":"Pt 11","key":"ppat.1009977.ref003","doi-asserted-by":"crossref","first-page":"1467","DOI":"10.1242\/jcs.111.11.1467","article-title":"The parasitophorous vacuole membrane surrounding Plasmodium and Toxoplasma: an unusual compartment in infected cells","volume":"111","author":"K Lingelbach","year":"1998","journal-title":"J Cell Sci"},{"issue":"2","key":"ppat.1009977.ref004","doi-asserted-by":"crossref","first-page":"493","DOI":"10.1111\/j.1365-2958.2006.05112.x","article-title":"Evidence for the involvement of Plasmodium falciparum proteins in the formation of new permeability pathways in the erythrocyte membrane","volume":"60","author":"S Baumeister","year":"2006","journal-title":"Mol Microbiol"},{"issue":"5","key":"ppat.1009977.ref005","doi-asserted-by":"crossref","first-page":"665","DOI":"10.1016\/j.cell.2011.05.002","article-title":"Malaria parasite clag3 genes determine channel-mediated nutrient uptake by infected red blood cells","volume":"145","author":"W Nguitragool","year":"2011","journal-title":"Cell"},{"key":"ppat.1009977.ref006","doi-asserted-by":"crossref","first-page":"e23217","DOI":"10.7554\/eLife.23217","article-title":"Plasmodium falciparum parasites deploy RhopH2 into the host erythrocyte to obtain nutrients, grow and replicate","volume":"6","author":"NA Counihan","year":"2017","journal-title":"eLife"},{"key":"ppat.1009977.ref007","first-page":"6","article-title":"An essential dual-function complex mediates erythrocyte invasion and channel-mediated nutrient uptake in malaria parasites","author":"D Ito","year":"2017","journal-title":"elife"},{"key":"ppat.1009977.ref008","doi-asserted-by":"crossref","first-page":"e23239","DOI":"10.7554\/eLife.23239","article-title":"The Plasmodium falciparum rhoptry protein RhopH3 plays essential roles in host cell invasion and nutrient uptake","volume":"6","author":"ES Sherling","year":"2017","journal-title":"eLife"},{"issue":"50","key":"ppat.1009977.ref009","doi-asserted-by":"crossref","first-page":"19987","DOI":"10.1073\/pnas.1309247110","article-title":"Maurer\u2019s clefts, the enigma of Plasmodium falciparum","volume":"110","author":"E Mundwiler-Pachlatko","year":"2013","journal-title":"Proc Natl Acad Sci U S A"},{"issue":"2","key":"ppat.1009977.ref010","doi-asserted-by":"crossref","first-page":"287","DOI":"10.1016\/S0092-8674(00)80207-X","article-title":"Targeted gene disruption shows that knobs enable malaria-infected red cells to cytoadhere under physiological shear stress","volume":"89","author":"BS Crabb","year":"1997","journal-title":"Cell"},{"issue":"4","key":"ppat.1009977.ref011","doi-asserted-by":"crossref","first-page":"1215","DOI":"10.1046\/j.1365-2958.2003.03784.x","article-title":"Characterization of the pathway for transport of the cytoadherence-mediating protein, PfEMP1, to the host cell surface in malaria parasite-infected erythrocytes","volume":"50","author":"N Kriek","year":"2003","journal-title":"Mol Microbiol"},{"issue":"2","key":"ppat.1009977.ref012","doi-asserted-by":"crossref","first-page":"e1560","DOI":"10.1371\/journal.pone.0001560","article-title":"The Plasmodium export element revisited","volume":"3","author":"JA Hiss","year":"2008","journal-title":"PLoS One"},{"issue":"12","key":"ppat.1009977.ref013","doi-asserted-by":"crossref","first-page":"1371","DOI":"10.1016\/S0020-7519(01)00253-3","article-title":"Plasmodium falciparum signal sequences: simply sequences or special signals?","volume":"31","author":"A Nacer","year":"2001","journal-title":"Int J Parasitol"},{"issue":"8","key":"ppat.1009977.ref014","doi-asserted-by":"crossref","first-page":"e1003546","DOI":"10.1371\/journal.ppat.1003546","article-title":"Identification of new PNEPs indicates a substantial non-PEXEL exportome and underpins common features in Plasmodium falciparum protein export","volume":"9","author":"A Heiber","year":"2013","journal-title":"PLoS Pathog"},{"issue":"4","key":"ppat.1009977.ref015","doi-asserted-by":"crossref","first-page":"1003","DOI":"10.1111\/j.1365-2958.2008.06582.x","article-title":"Sequence requirements for the export of the Plasmodium falciparum Maurer\u2019s clefts protein REX2","volume":"71","author":"S Haase","year":"2009","journal-title":"Mol Microbiol"},{"issue":"5703","key":"ppat.1009977.ref016","doi-asserted-by":"crossref","first-page":"1934","DOI":"10.1126\/science.1102737","article-title":"A host-targeting signal in virulence proteins reveals a secretome in malarial infection","volume":"306","author":"NL Hiller","year":"2004","journal-title":"Science"},{"issue":"5703","key":"ppat.1009977.ref017","doi-asserted-by":"crossref","first-page":"1930","DOI":"10.1126\/science.1102452","article-title":"Targeting malaria virulence and remodeling proteins to the host erythrocyte","volume":"306","author":"M Marti","year":"2004","journal-title":"Science"},{"issue":"4","key":"ppat.1009977.ref018","doi-asserted-by":"crossref","first-page":"e13009","DOI":"10.1111\/cmi.13009","article-title":"Illuminating how malaria parasites export proteins into host erythrocytes","volume":"21","author":"KM Matthews","year":"2019","journal-title":"Cell Microbiol"},{"issue":"2","key":"ppat.1009977.ref019","doi-asserted-by":"crossref","first-page":"R12","DOI":"10.1186\/gb-2006-7-2-r12","article-title":"Lineage-specific expansion of proteins exported to erythrocytes in malaria parasites","volume":"7","author":"TJ Sargeant","year":"2006","journal-title":"Genome Biol"},{"issue":"6","key":"ppat.1009977.ref020","doi-asserted-by":"crossref","first-page":"e1000084","DOI":"10.1371\/journal.ppat.1000084","article-title":"The malaria secretome: from algorithms to essential function in blood stage infection","volume":"4","author":"C van Ooij","year":"2008","journal-title":"PLoS Pathog"},{"issue":"5","key":"ppat.1009977.ref021","doi-asserted-by":"crossref","first-page":"532","DOI":"10.1111\/tra.12053","article-title":"Role of plasmepsin V in export of diverse protein families from the Plasmodium falciparum exportome","volume":"14","author":"JA Boddey","year":"2013","journal-title":"Traffic"},{"issue":"2","key":"ppat.1009977.ref022","doi-asserted-by":"crossref","first-page":"301","DOI":"10.1111\/mmi.13024","article-title":"The Plasmodium falciparum exportome contains non-canonical PEXEL\/HT proteins","volume":"97","author":"J Schulze","year":"2015","journal-title":"Mol Microbiol"},{"issue":"2","key":"ppat.1009977.ref023","doi-asserted-by":"crossref","first-page":"107","DOI":"10.1016\/j.molbiopara.2008.04.011","article-title":"N-terminal processing of proteins exported by malaria parasites","volume":"160","author":"HH Chang","year":"2008","journal-title":"Mol Biochem Parasitol"},{"issue":"3","key":"ppat.1009977.ref024","doi-asserted-by":"crossref","first-page":"285","DOI":"10.1111\/j.1600-0854.2008.00864.x","article-title":"Role of the Plasmodium export element in trafficking parasite proteins to the infected erythrocyte","volume":"10","author":"JA Boddey","year":"2009","journal-title":"Traffic"},{"issue":"7281","key":"ppat.1009977.ref025","doi-asserted-by":"crossref","first-page":"627","DOI":"10.1038\/nature08728","article-title":"An aspartyl protease directs malaria effector proteins to the host cell","volume":"463","author":"JA Boddey","year":"2010","journal-title":"Nature"},{"issue":"9","key":"ppat.1009977.ref026","doi-asserted-by":"crossref","first-page":"1010","DOI":"10.1038\/s41564-018-0219-2","article-title":"Plasmepsin V cleaves malaria effector proteins in a distinct endoplasmic reticulum translocation interactome for export to the erythrocyte","volume":"3","author":"DS Marapana","year":"2018","journal-title":"Nat Microbiol"},{"issue":"Pt 1","key":"ppat.1009977.ref027","doi-asserted-by":"crossref","first-page":"307","DOI":"10.1042\/bj3150307","article-title":"Protein sorting in Plasmodium falciparum-infected red blood cells permeabilized with the pore-forming protein streptolysin O","volume":"315","author":"I Ansorge","year":"1996","journal-title":"Biochem J"},{"issue":"3","key":"ppat.1009977.ref028","doi-asserted-by":"crossref","first-page":"613","DOI":"10.1111\/j.1365-2958.2008.06552.x","article-title":"Protein unfolding is an essential requirement for transport across the parasitophorous vacuolar membrane of Plasmodium falciparum","volume":"71","author":"N Gehde","year":"2009","journal-title":"Mol Microbiol"},{"issue":"7249","key":"ppat.1009977.ref029","doi-asserted-by":"crossref","first-page":"945","DOI":"10.1038\/nature08104","article-title":"A newly discovered protein export machine in malaria parasites","volume":"459","author":"TF de Koning-Ward","year":"2009","journal-title":"Nature"},{"issue":"11","key":"ppat.1009977.ref030","doi-asserted-by":"crossref","first-page":"7871","DOI":"10.1074\/jbc.M111.328591","article-title":"Biosynthesis, localization, and macromolecular arrangement of the Plasmodium falciparum translocon of exported proteins (PTEX)","volume":"287","author":"HE Bullen","year":"2012","journal-title":"J Biol Chem"},{"issue":"11","key":"ppat.1009977.ref031","doi-asserted-by":"crossref","first-page":"1551","DOI":"10.1111\/cmi.12596","article-title":"Proteomic analysis reveals novel proteins associated with the Plasmodium protein exporter PTEX and a loss of complex stability upon truncation of the core PTEX component, PTEX150","volume":"18","author":"B Elsworth","year":"2016","journal-title":"Cell Microbiol"},{"issue":"7721","key":"ppat.1009977.ref032","doi-asserted-by":"crossref","first-page":"70","DOI":"10.1038\/s41586-018-0469-4","article-title":"Malaria parasite translocon structure and mechanism of effector export","volume":"561","author":"CM Ho","year":"2018","journal-title":"Nature"},{"issue":"3","key":"ppat.1009977.ref033","doi-asserted-by":"crossref","first-page":"239","DOI":"10.1093\/jb\/mvy099","article-title":"The N-terminus of EXP2 forms the membrane-associated pore of the protein exporting translocon PTEX in Plasmodium falciparum","volume":"165","author":"PR Sanders","year":"2019","journal-title":"J Biochem"},{"issue":"3","key":"ppat.1009977.ref034","doi-asserted-by":"crossref","DOI":"10.1128\/mBio.01106-19","article-title":"Uncoupling the threading and unfoldase actions of Plasmodium HSP101 reveals differences in export between soluble and insoluble proteins","volume":"10","author":"KM Matthews","year":"2019","journal-title":"mBio"},{"issue":"7511","key":"ppat.1009977.ref035","doi-asserted-by":"crossref","first-page":"592","DOI":"10.1038\/nature13574","article-title":"PTEX component HSP101 mediates export of diverse malaria effectors into host erythrocytes","volume":"511","author":"JR Beck","year":"2014","journal-title":"Nature"},{"issue":"7511","key":"ppat.1009977.ref036","doi-asserted-by":"crossref","first-page":"587","DOI":"10.1038\/nature13555","article-title":"PTEX is an essential nexus for protein export in malaria parasites","volume":"511","author":"B Elsworth","year":"2014","journal-title":"Nature"},{"issue":"11","key":"ppat.1009977.ref037","doi-asserted-by":"crossref","first-page":"e0204785","DOI":"10.1371\/journal.pone.0204785","article-title":"Knockdown of the translocon protein EXP2 in Plasmodium falciparum reduces growth and protein export","volume":"13","author":"SC Charnaud","year":"2018","journal-title":"PLoS One"},{"issue":"10","key":"ppat.1009977.ref038","doi-asserted-by":"crossref","first-page":"1090","DOI":"10.1038\/s41564-018-0222-7","article-title":"EXP2 is a nutrient-permeable channel in the vacuolar membrane of Plasmodium and is essential for protein export via PTEX","volume":"3","author":"M Garten","year":"2018","journal-title":"Nat Microbiol"},{"issue":"7281","key":"ppat.1009977.ref039","doi-asserted-by":"crossref","first-page":"632","DOI":"10.1038\/nature08726","article-title":"Plasmepsin V licenses Plasmodium proteins for export into the host erythrocyte","volume":"463","author":"I Russo","year":"2010","journal-title":"Nature"},{"issue":"9","key":"ppat.1009977.ref040","doi-asserted-by":"crossref","first-page":"617","DOI":"10.1038\/nrmicro2420","article-title":"Moving in and renovating: exporting proteins from Plasmodium into host erythrocytes","volume":"8","author":"DE Goldberg","year":"2010","journal-title":"Nat Rev Microbiol"},{"key":"ppat.1009977.ref041","doi-asserted-by":"crossref","first-page":"12532","DOI":"10.1038\/srep12532","article-title":"The Plasmodium berghei translocon of exported proteins reveals spatiotemporal dynamics of tubular extensions","volume":"5","author":"JM Matz","year":"2015","journal-title":"Sci Rep"},{"issue":"8","key":"ppat.1009977.ref042","doi-asserted-by":"crossref","first-page":"605","DOI":"10.1111\/tra.12577","article-title":"Spatial organization of protein export in malaria parasite blood stages","volume":"19","author":"SC Charnaud","year":"2018","journal-title":"Traffic"},{"issue":"5","key":"ppat.1009977.ref043","doi-asserted-by":"crossref","first-page":"e13168","DOI":"10.1111\/cmi.13168","article-title":"EXP1 is required for organisation of EXP2 in the intraerythrocytic malaria parasite vacuole","volume":"22","author":"T Nessel","year":"2020","journal-title":"Cell Microbiol"},{"issue":"8","key":"ppat.1009977.ref044","doi-asserted-by":"crossref","first-page":"e73783","DOI":"10.1371\/journal.pone.0073783","article-title":"Inducible knockdown of Plasmodium gene expression using the glmS ribozyme","volume":"8","author":"P Prommana","year":"2013","journal-title":"PLoS One"},{"issue":"11","key":"ppat.1009977.ref045","doi-asserted-by":"crossref","first-page":"2037","DOI":"10.1111\/febs.14463","article-title":"The malaria PTEX component PTEX88 interacts most closely with HSP101 at the host-parasite interface","volume":"285","author":"SA Chisholm","year":"2018","journal-title":"FEBS J"},{"issue":"1","key":"ppat.1009977.ref046","doi-asserted-by":"crossref","first-page":"167","DOI":"10.1042\/BJ20061725","article-title":"Selective permeabilization of the host cell membrane of Plasmodium falciparum-infected red blood cells with streptolysin O and equinatoxin II","volume":"403","author":"KE Jackson","year":"2007","journal-title":"Biochem J"},{"key":"ppat.1009977.ref047","doi-asserted-by":"crossref","first-page":"16044","DOI":"10.1038\/ncomms16044","article-title":"An exported protein-interacting complex involved in the trafficking of virulence determinants in Plasmodium-infected erythrocytes","volume":"8","author":"S Batinovic","year":"2017","journal-title":"Nat Commun"},{"issue":"1","key":"ppat.1009977.ref048","doi-asserted-by":"crossref","first-page":"E5","DOI":"10.1371\/journal.pbio.0000005","article-title":"The transcriptome of the intraerythrocytic developmental cycle of Plasmodium falciparum","volume":"1","author":"Z Bozdech","year":"2003","journal-title":"PLoS Biol"},{"issue":"1","key":"ppat.1009977.ref049","doi-asserted-by":"crossref","first-page":"12","DOI":"10.1111\/j.1365-2958.2009.07026.x","article-title":"New insights into the blood-stage transcriptome of Plasmodium falciparum using RNA-Seq","volume":"76","author":"TD Otto","year":"2010","journal-title":"Mol Microbiol"},{"issue":"Pt 3","key":"ppat.1009977.ref050","doi-asserted-by":"crossref","first-page":"821","DOI":"10.1042\/bj3000821","article-title":"Brefeldin A inhibits transport of the glycophorin-binding protein from Plasmodium falciparum into the host erythrocyte","volume":"300","author":"J Benting","year":"1994","journal-title":"Biochem J"},{"issue":"1\u20132","key":"ppat.1009977.ref051","doi-asserted-by":"crossref","first-page":"185","DOI":"10.1016\/0166-6851(92)90020-K","article-title":"Brefeldin A inhibits protein secretion and parasite maturation in the ring stage of Plasmodium falciparum","volume":"53","author":"JL Crary","year":"1992","journal-title":"Mol Biochem Parasitol"},{"issue":"11","key":"ppat.1009977.ref052","doi-asserted-by":"crossref","first-page":"e112571","DOI":"10.1371\/journal.pone.0112571","article-title":"Plasmodium falciparum transfected with ultra bright NanoLuc luciferase offers high sensitivity detection for the screening of growth and cellular trafficking inhibitors","volume":"9","author":"MF Azevedo","year":"2014","journal-title":"PLoS One"},{"issue":"5","key":"ppat.1009977.ref053","doi-asserted-by":"crossref","first-page":"e1005618","DOI":"10.1371\/journal.ppat.1005618","article-title":"Stable translocation intermediates jam global protein export in Plasmodium falciparum parasites and link the PTEX component EXP2 with translocation activity","volume":"12","author":"P Mesen-Ramirez","year":"2016","journal-title":"PLoS Pathog"},{"issue":"4","key":"ppat.1009977.ref054","doi-asserted-by":"crossref","first-page":"835","DOI":"10.1111\/mmi.12133","article-title":"The C-terminal portion of the cleaved HT motif is necessary and sufficient to mediate export of proteins from the malaria parasite into its host cell","volume":"87","author":"SJ Tarr","year":"2013","journal-title":"Mol Microbiol"},{"issue":"8","key":"ppat.1009977.ref055","doi-asserted-by":"crossref","first-page":"590","DOI":"10.1038\/nsmb.3061","article-title":"Structural basis for plasmepsin V inhibition that blocks export of malaria proteins to human erythrocytes","volume":"22","author":"AN Hodder","year":"2015","journal-title":"Nat Struct Mol Biol"},{"issue":"5","key":"ppat.1009977.ref056","doi-asserted-by":"crossref","first-page":"717","DOI":"10.1016\/j.chom.2012.09.010","article-title":"Uncovering common principles in protein export of malaria parasites","volume":"12","author":"C Gruring","year":"2012","journal-title":"Cell Host Microbe"},{"issue":"4","key":"ppat.1009977.ref057","doi-asserted-by":"crossref","first-page":"e1009394","DOI":"10.1371\/journal.ppat.1009394","article-title":"Transport mechanisms at the malaria parasite-host cell interface","volume":"17","author":"JR Beck","year":"2021","journal-title":"PLoS Pathog"},{"issue":"1","key":"ppat.1009977.ref058","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.abb.2012.01.012","article-title":"Aggregate reactivation mediated by the Hsp100 chaperones","volume":"520","author":"M Zolkiewski","year":"2012","journal-title":"Arch Biochem Biophys"},{"issue":"46","key":"ppat.1009977.ref059","doi-asserted-by":"crossref","first-page":"38170","DOI":"10.1074\/jbc.M506149200","article-title":"Substrate binding to the molecular chaperone Hsp104 and its regulation by nucleotides","volume":"280","author":"B Bosl","year":"2005","journal-title":"J Biol Chem"},{"issue":"12","key":"ppat.1009977.ref060","doi-asserted-by":"crossref","first-page":"2483","DOI":"10.1111\/j.1462-5822.2008.01223.x","article-title":"A conditional export system provides new insights into protein export in Plasmodium falciparum-infected erythrocytes","volume":"10","author":"T Saridaki","year":"2008","journal-title":"Cell Microbiol"},{"issue":"2","key":"ppat.1009977.ref061","doi-asserted-by":"crossref","first-page":"258","DOI":"10.1016\/j.molbiopara.2005.04.005","article-title":"Function of the plasmodium export element can be blocked by green fluorescent protein","volume":"142","author":"E Knuepfer","year":"2005","journal-title":"Mol Biochem Parasitol"},{"issue":"10","key":"ppat.1009977.ref062","doi-asserted-by":"crossref","first-page":"514","DOI":"10.1016\/j.pt.2015.06.010","article-title":"Critical steps in protein export of Plasmodium falciparum blood stages","volume":"31","author":"T Spielmann","year":"2015","journal-title":"Trends Parasitol"},{"key":"ppat.1009977.ref063","article-title":"Membrane translocation at the ER: with a little help from my friends","author":"S O\u2019Keefe","year":"2020","journal-title":"FEBS J"},{"issue":"6","key":"ppat.1009977.ref064","doi-asserted-by":"crossref","first-page":"865","DOI":"10.3390\/microorganisms8060865","article-title":"Crossing the vacuolar rubicon: structural insights into effector protein trafficking in apicomplexan parasites","volume":"8","author":"PF Egea","year":"2020","journal-title":"Microorganisms"},{"issue":"12","key":"ppat.1009977.ref065","doi-asserted-by":"crossref","first-page":"1347","DOI":"10.1038\/nsmb.2442","article-title":"Hsp70 proteins bind Hsp100 regulatory M domains to activate AAA+ disaggregase at aggregate surfaces","volume":"19","author":"F Seyffer","year":"2012","journal-title":"Nat Struct Mol Biol"},{"key":"ppat.1009977.ref066","doi-asserted-by":"crossref","first-page":"22","DOI":"10.3389\/fmolb.2015.00022","article-title":"Cooperation of Hsp70 and Hsp100 chaperone machines in protein disaggregation","volume":"2","author":"A Mogk","year":"2015","journal-title":"Front Mol Biosci"},{"issue":"4254","key":"ppat.1009977.ref067","doi-asserted-by":"crossref","first-page":"673","DOI":"10.1126\/science.781840","article-title":"Human malaria parasites in continuous culture","volume":"193","author":"W Trager","year":"1976","journal-title":"Science"},{"issue":"3","key":"ppat.1009977.ref068","doi-asserted-by":"crossref","first-page":"850","DOI":"10.1093\/nar\/29.3.850","article-title":"Transformation of malaria parasites by the spontaneous uptake and expression of DNA from human erythrocytes","volume":"29","author":"K Deitsch","year":"2001","journal-title":"Nucleic Acids Res"},{"key":"ppat.1009977.ref069","doi-asserted-by":"crossref","first-page":"58","DOI":"10.1186\/1475-2875-12-58","article-title":"Evaluation of bioluminescence-based assays of anti-malarial drug activity","volume":"12","author":"S Hasenkamp","year":"2013","journal-title":"Malar J"},{"issue":"1","key":"ppat.1009977.ref070","doi-asserted-by":"crossref","first-page":"13","DOI":"10.1016\/j.molbiopara.2004.05.009","article-title":"Localization of organellar proteins in Plasmodium falciparum using a novel set of transfection vectors and a new immunofluorescence fixation method","volume":"137","author":"CJ Tonkin","year":"2004","journal-title":"Mol Biochem Parasitol"},{"issue":"2","key":"ppat.1009977.ref071","doi-asserted-by":"crossref","first-page":"e0149296","DOI":"10.1371\/journal.pone.0149296","article-title":"Contrasting inducible knockdown of the auxiliary PTEX component PTEX88 in P. falciparum and P. berghei unmasks a role in parasite virulence","volume":"11","author":"SA Chisholm","year":"2016","journal-title":"PLoS One"},{"issue":"4","key":"ppat.1009977.ref072","first-page":"e00589","article-title":"Toxoplasma gondii parasitophorous vacuole membrane-associated dense granule proteins orchestrate chronic infection and GRA12 underpins resistance to host gamma Interferon","volume":"10","author":"BA Fox","year":"2019","journal-title":"mBio"}],"updated-by":[{"DOI":"10.1371\/journal.ppat.1009977","type":"new_version","label":"New version","source":"publisher","updated":{"date-parts":[[2022,3,4]],"date-time":"2022-03-04T00:00:00Z","timestamp":1646352000000}},{"DOI":"10.1371\/journal.ppat.1010757","type":"correction","label":"Correction","source":"publisher","updated":{"date-parts":[[2022,8,5]],"date-time":"2022-08-05T00:00:00Z","timestamp":1659657600000}}],"container-title":["PLOS Pathogens"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/dx.plos.org\/10.1371\/journal.ppat.1009977","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,3,4]],"date-time":"2022-03-04T13:46:38Z","timestamp":1646401598000},"score":1,"resource":{"primary":{"URL":"https:\/\/dx.plos.org\/10.1371\/journal.ppat.1009977"}},"subtitle":[],"editor":[{"given":"Michael J.","family":"Blackman","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2022,2,22]]},"references-count":72,"journal-issue":{"issue":"2","published-online":{"date-parts":[[2022,2,22]]}},"URL":"https:\/\/doi.org\/10.1371\/journal.ppat.1009977","relation":{"has-preprint":[{"id-type":"doi","id":"10.1101\/2021.09.29.462329","asserted-by":"object"}]},"ISSN":["1553-7374"],"issn-type":[{"value":"1553-7374","type":"electronic"}],"subject":[],"published":{"date-parts":[[2022,2,22]]}}}