{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T23:55:15Z","timestamp":1790898915840,"version":"4.1.0"},"update-to":[{"DOI":"10.1371\/journal.ppat.1009980","type":"new_version","label":"New version","source":"publisher","updated":{"date-parts":[[2022,1,10]],"date-time":"2022-01-10T00:00:00Z","timestamp":1641772800000}}],"reference-count":72,"publisher":"Public Library of Science (PLoS)","issue":"12","license":[{"start":{"date-parts":[[2021,12,28]],"date-time":"2021-12-28T00:00:00Z","timestamp":1640649600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001659","name":"Deutsche Forschungsgemeinschaft","doi-asserted-by":"publisher","award":["SFB987"],"award-info":[{"award-number":["SFB987"]}],"id":[{"id":"10.13039\/501100001659","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100010198","name":"Ministerio de Econom\u00eda, Industria y Competitividad, Gobierno de Espa\u00f1a","doi-asserted-by":"publisher","award":["SAF2013-47570-P"],"award-info":[{"award-number":["SAF2013-47570-P"]}],"id":[{"id":"10.13039\/501100010198","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100010198","name":"Ministerio de Econom\u00eda, Industria y Competitividad, Gobierno de Espa\u00f1a","doi-asserted-by":"publisher","award":["SAF2017-86188-P"],"award-info":[{"award-number":["SAF2017-86188-P"]}],"id":[{"id":"10.13039\/501100010198","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100011698","name":"Junta de Comunidades de Castilla-La Mancha","doi-asserted-by":"publisher","award":["SBPLY\/19\/180501\/000114"],"award-info":[{"award-number":["SBPLY\/19\/180501\/000114"]}],"id":[{"id":"10.13039\/501100011698","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100011698","name":"Junta de Comunidades de Castilla-La Mancha","doi-asserted-by":"publisher","award":["SBPLY\/19\/180501\/000356"],"award-info":[{"award-number":["SBPLY\/19\/180501\/000356"]}],"id":[{"id":"10.13039\/501100011698","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["www.plospathogens.org"],"crossmark-restriction":false},"short-container-title":["PLoS Pathog"],"abstract":"<jats:p><jats:italic>Candida glabrata<\/jats:italic> is an opportunistic pathogenic yeast frequently causing infections in humans. Though it lacks typical virulence factors such as hyphal development, <jats:italic>C<\/jats:italic>. <jats:italic>glabrata<\/jats:italic> contains a remarkably large and diverse set of putative wall adhesins that is crucial for its success as pathogen. Here, we present an analysis of putative adhesins from the homology clusters V and VI. First, sequence similarity network analysis revealed relationships between cluster V and VI adhesins and <jats:italic>S<\/jats:italic>. <jats:italic>cerevisiae<\/jats:italic> haze protective factors (Hpf). Crystal structures of A-regions from cluster VI adhesins Awp1 and Awp3b reveal a parallel right-handed \u03b2-helix domain that is linked to a C-terminal \u03b2-sandwich. Structure solution of the A-region of Awp3b via single wavelength anomalous diffraction phasing revealed the largest known lanthanide cluster with 21 Gd<jats:sup>3+<\/jats:sup> ions. Awp1-A and Awp3b-A show structural similarity to pectate lyases but binding to neither carbohydrates nor Ca<jats:sup>2+<\/jats:sup> was observed. Phenotypic analysis of <jats:italic>awp1<\/jats:italic>\u0394, <jats:italic>awp3<\/jats:italic>\u0394, and <jats:italic>awp1<\/jats:italic>,<jats:italic>3<\/jats:italic>\u0394 double mutants did also not confirm their role as adhesins. In contrast, deletion mutants of the cluster V adhesin Awp2 in the hyperadhesive clinical isolate PEU382 demonstrated its importance for adhesion to polystyrene or glass, biofilm formation, cell aggregation and other cell surface-related phenotypes. Together with cluster III and VII adhesins our study shows that <jats:italic>C<\/jats:italic>. <jats:italic>glabrata<\/jats:italic> CBS138 can rely on a set of 42 Awp1-related adhesins with \u03b2-helix\/\u03b1-crystallin domain architecture for modifying the surface characteristics of its cell wall.<\/jats:p>","DOI":"10.1371\/journal.ppat.1009980","type":"journal-article","created":{"date-parts":[[2021,12,28]],"date-time":"2021-12-28T19:50:42Z","timestamp":1640721042000},"page":"e1009980","update-policy":"https:\/\/doi.org\/10.1371\/journal.ppat.corrections_policy","source":"Crossref","is-referenced-by-count":26,"title":["A novel class of Candida glabrata cell wall proteins with \u03b2-helix fold mediates adhesion in clinical isolates"],"prefix":"10.1371","volume":"17","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-3980-1695","authenticated-orcid":true,"given":"Viktoria","family":"Reithofer","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4405-9453","authenticated-orcid":true,"given":"Jordan","family":"Fern\u00e1ndez-Pereira","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6879-1609","authenticated-orcid":true,"given":"Mar\u00eda","family":"Alvarado","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3112-6947","authenticated-orcid":true,"given":"Piet","family":"de Groot","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4272-4026","authenticated-orcid":true,"given":"Lars-Oliver","family":"Essen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"340","published-online":{"date-parts":[[2021,12,28]]},"reference":[{"key":"ppat.1009980.ref001","doi-asserted-by":"crossref","first-page":"470","DOI":"10.1128\/EC.00364-12","article-title":"Adhesins in human fungal pathogens: Glue with plenty of stick","author":"PWJ de Groot","year":"2013","journal-title":"Eukaryotic Cell"},{"key":"ppat.1009980.ref002","doi-asserted-by":"crossref","first-page":"378","DOI":"10.1016\/j.mib.2005.06.012","article-title":"A yeast by any other name: Candida glabrata and its interaction with the host","volume":"8","author":"R Kaur","year":"2005","journal-title":"Curr Opin 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