{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T06:23:48Z","timestamp":1790663028253,"version":"4.1.0"},"update-to":[{"DOI":"10.1371\/journal.ppat.1010042","type":"new_version","label":"New version","source":"publisher","updated":{"date-parts":[[2021,11,18]],"date-time":"2021-11-18T00:00:00Z","timestamp":1637193600000}}],"reference-count":27,"publisher":"Public Library of Science (PLoS)","issue":"11","license":[{"start":{"date-parts":[[2021,11,8]],"date-time":"2021-11-08T00:00:00Z","timestamp":1636329600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/publicdomain\/zero\/1.0\/"}],"funder":[{"name":"BMFG","award":["INV 001763"],"award-info":[{"award-number":["INV 001763"]}]},{"DOI":"10.13039\/100006492","name":"Division of Intramural Research, National Institute of Allergy and Infectious Diseases","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100006492","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["www.plospathogens.org"],"crossmark-restriction":false},"short-container-title":["PLoS Pathog"],"abstract":"<jats:p>\n                    Rare and potent monoclonal antibodies (mAbs) against the\n                    <jats:italic>Plasmodium falciparum<\/jats:italic>\n                    (Pf) circumsporozoite protein (CSP) on infective sporozoites (SPZ) preferentially bind the PfCSP junctional tetrapeptide NPDP or NVDP minor repeats while cross-reacting with NANP central repeats\n                    <jats:italic>in vitro<\/jats:italic>\n                    . The extent to which each of these epitopes is required for protection\n                    <jats:italic>in vivo<\/jats:italic>\n                    is unknown. Here, we assessed whether junction-, minor repeat- and central repeat-preferring human mAbs (CIS43, L9 and 317 respectively) bound and protected against\n                    <jats:italic>in vivo<\/jats:italic>\n                    challenge with transgenic\n                    <jats:italic>P<\/jats:italic>\n                    .\n                    <jats:italic>berghei<\/jats:italic>\n                    (Pb) SPZ expressing either PfCSP with the junction and minor repeats knocked out (KO), or PbCSP with the junction and minor repeats knocked in (KI).\n                    <jats:italic>In vivo<\/jats:italic>\n                    protection studies showed that the junction and minor repeats are necessary and sufficient for CIS43 and L9 to neutralize KO and KI SPZ, respectively. In contrast, 317 required major repeats for\n                    <jats:italic>in vivo<\/jats:italic>\n                    protection. These data establish that human mAbs can prevent malaria infection by targeting three different protective epitopes (NPDP, NVDP, NANP) in the PfCSP repeat region. This report will inform vaccine development and the use of mAbs to passively prevent malaria.\n                  <\/jats:p>","DOI":"10.1371\/journal.ppat.1010042","type":"journal-article","created":{"date-parts":[[2021,11,8]],"date-time":"2021-11-08T13:42:00Z","timestamp":1636378920000},"page":"e1010042","update-policy":"https:\/\/doi.org\/10.1371\/journal.ppat.corrections_policy","source":"Crossref","is-referenced-by-count":45,"title":["The P. falciparum CSP repeat region contains three distinct epitopes required for protection by antibodies in vivo"],"prefix":"10.1371","volume":"17","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-7230-3834","authenticated-orcid":true,"given":"Yevel","family":"Flores-Garcia","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8026-8044","authenticated-orcid":true,"given":"Lawrence 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