{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,24]],"date-time":"2026-10-24T01:45:32Z","timestamp":1792806332005,"version":"4.5.0"},"update-to":[{"DOI":"10.1371\/journal.ppat.1010096","type":"new_version","label":"New version","source":"publisher","updated":{"date-parts":[[2021,12,2]],"date-time":"2021-12-02T00:00:00Z","timestamp":1638403200000}}],"reference-count":126,"publisher":"Public Library of Science (PLoS)","issue":"11","license":[{"start":{"date-parts":[[2021,11,18]],"date-time":"2021-11-18T00:00:00Z","timestamp":1637193600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100002915","name":"Fondation pour la Recherche M\u00e9dicale","doi-asserted-by":"crossref","award":["FRM EQ20170336725"],"award-info":[{"award-number":["FRM EQ20170336725"]}],"id":[{"id":"10.13039\/501100002915","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/100017923","name":"LabEx ParaFrap","doi-asserted-by":"crossref","award":["ANR-11-LABX-0024"],"award-info":[{"award-number":["ANR-11-LABX-0024"]}],"id":[{"id":"10.13039\/100017923","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100001665","name":"Agence Nationale de la Recherche","doi-asserted-by":"crossref","award":["ANR-19-CE15-0023"],"award-info":[{"award-number":["ANR-19-CE15-0023"]}],"id":[{"id":"10.13039\/501100001665","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":["www.plospathogens.org"],"crossmark-restriction":false},"short-container-title":["PLoS Pathog"],"abstract":"<jats:p>\n                    Iron-sulfur (Fe-S) clusters are one of the most ancient and ubiquitous prosthetic groups, and they are required by a variety of proteins involved in important metabolic processes. Apicomplexan parasites have inherited different plastidic and mitochondrial Fe-S clusters biosynthesis pathways through endosymbiosis. We have investigated the relative contributions of these pathways to the fitness of\n                    <jats:italic>Toxoplasma gondii<\/jats:italic>\n                    , an apicomplexan parasite causing disease in humans, by generating specific mutants. Phenotypic analysis and quantitative proteomics allowed us to highlight notable differences in these mutants. Both Fe-S cluster synthesis pathways are necessary for optimal parasite growth in vitro, but their disruption leads to markedly different fates: impairment of the plastidic pathway leads to a loss of the organelle and to parasite death, while disruption of the mitochondrial pathway trigger differentiation into a stress resistance stage. This highlights that otherwise similar biochemical pathways hosted by different sub-cellular compartments can have very different contributions to the biology of the parasites, which is something to consider when exploring novel strategies for therapeutic intervention.\n                  <\/jats:p>","DOI":"10.1371\/journal.ppat.1010096","type":"journal-article","created":{"date-parts":[[2021,11,18]],"date-time":"2021-11-18T14:06:14Z","timestamp":1637244374000},"page":"e1010096","update-policy":"https:\/\/doi.org\/10.1371\/journal.ppat.corrections_policy","source":"Crossref","is-referenced-by-count":43,"title":["Differential contribution of two organelles of endosymbiotic origin to iron-sulfur cluster synthesis and overall fitness in Toxoplasma"],"prefix":"10.1371","volume":"17","author":[{"given":"Sarah","family":"Pamukcu","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2838-5021","authenticated-orcid":true,"given":"Aude","family":"Cerutti","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8616-6498","authenticated-orcid":true,"given":"Yann","family":"Bordat","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sonia","family":"Hem","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7666-7475","authenticated-orcid":true,"given":"Val\u00e9rie","family":"Rofidal","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1853-1494","authenticated-orcid":true,"given":"S\u00e9bastien","family":"Besteiro","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"340","published-online":{"date-parts":[[2021,11,18]]},"reference":[{"key":"ppat.1010096.ref001","doi-asserted-by":"crossref","first-page":"38","DOI":"10.1016\/j.mib.2014.09.008","article-title":"Endosymbiotic theory for organelle origins","volume":"22","author":"V Zimorski","year":"2014","journal-title":"Current Opinion in Microbiology"},{"key":"ppat.1010096.ref002","doi-asserted-by":"crossref","first-page":"745","DOI":"10.1038\/s41556-018-0124-1","article-title":"The multifaceted contributions of mitochondria to cellular metabolism","volume":"20","author":"JB Spinelli","year":"2018","journal-title":"Nat Cell Biol"},{"key":"ppat.1010096.ref003","doi-asserted-by":"crossref","first-page":"73","DOI":"10.1007\/978-1-4939-8654-5_5","volume-title":"Plastids","author":"N Rolland","year":"2018"},{"key":"ppat.1010096.ref004","doi-asserted-by":"crossref","first-page":"1847","DOI":"10.1093\/pcp\/pcq147","article-title":"Versatile roles of plastids in plant growth and development","volume":"51","author":"T Inaba","year":"2010","journal-title":"Plant and Cell Physiology"},{"key":"ppat.1010096.ref005","doi-asserted-by":"crossref","first-page":"482","DOI":"10.1038\/381482a0","article-title":"Plastid in human parasites","volume":"381","author":"GI McFadden","year":"1996","journal-title":"Nature"},{"key":"ppat.1010096.ref006","doi-asserted-by":"crossref","first-page":"407","DOI":"10.1038\/37132","article-title":"A plastid organelle as a drug target in apicomplexan parasites","volume":"390","author":"ME Fichera","year":"1997","journal-title":"Nature"},{"key":"ppat.1010096.ref007","doi-asserted-by":"crossref","first-page":"583","DOI":"10.1146\/annurev-arplant-050312-120144","article-title":"The number, speed, and impact of plastid endosymbioses in eukaryotic evolution","volume":"64","author":"PJ Keeling","year":"2013","journal-title":"Annu Rev Plant Biol"},{"key":"ppat.1010096.ref008","doi-asserted-by":"crossref","first-page":"R515","DOI":"10.1016\/j.cub.2015.04.033","article-title":"Protein import and the origin of red complex plastids","volume":"25","author":"SB Gould","year":"2015","journal-title":"Current Biology"},{"key":"ppat.1010096.ref009","doi-asserted-by":"crossref","first-page":"271","DOI":"10.1146\/annurev-micro-092412-155741","article-title":"The algal past and parasite present of the apicoplast","volume":"67","author":"GG van Dooren","year":"2013","journal-title":"Annu Rev Microbiol"},{"key":"ppat.1010096.ref010","doi-asserted-by":"crossref","first-page":"452","DOI":"10.1016\/j.mib.2013.07.003","article-title":"The metabolic roles of the endosymbiotic organelles of Toxoplasma and Plasmodium spp","volume":"16","author":"L Sheiner","year":"2013","journal-title":"Curr Opin Microbiol"},{"key":"ppat.1010096.ref011","doi-asserted-by":"crossref","first-page":"145","DOI":"10.1016\/bs.abr.2017.06.007","article-title":"The dark side of the chloroplast: biogenesis, metabolism and membrane biology of the apicoplast","author":"GG van Dooren","year":"2017","journal-title":"Advances in Botanical Research. 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