{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,20]],"date-time":"2026-03-20T23:44:57Z","timestamp":1774050297193,"version":"3.50.1"},"update-to":[{"DOI":"10.1371\/journal.ppat.1010114","type":"new_version","label":"New version","source":"publisher","updated":{"date-parts":[[2021,12,9]],"date-time":"2021-12-09T00:00:00Z","timestamp":1639008000000}}],"reference-count":52,"publisher":"Public Library of Science (PLoS)","issue":"11","license":[{"start":{"date-parts":[[2021,11,29]],"date-time":"2021-11-29T00:00:00Z","timestamp":1638144000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"F.W.O.-Vlaanderen","award":["G0C9720N"],"award-info":[{"award-number":["G0C9720N"]}]},{"DOI":"10.13039\/501100004497","name":"Onderzoeksraad, KU Leuven","doi-asserted-by":"publisher","award":["C16\/17\/010"],"award-info":[{"award-number":["C16\/17\/010"]}],"id":[{"id":"10.13039\/501100004497","id-type":"DOI","asserted-by":"publisher"}]},{"name":"F.W.O.-Vlaanderen","award":["Aspirant PhD fellowship"],"award-info":[{"award-number":["Aspirant PhD fellowship"]}]},{"name":"F.W.O.-Vlaanderen","award":["Postdoc fellowship"],"award-info":[{"award-number":["Postdoc fellowship"]}]},{"name":"L'Or\u00e9al-Unesco Women for Sciences","award":["PhD fellowship"],"award-info":[{"award-number":["PhD fellowship"]}]},{"name":"F.W.O. Vlaanderen","award":["PhD fellowship"],"award-info":[{"award-number":["PhD fellowship"]}]}],"content-domain":{"domain":["www.plospathogens.org"],"crossmark-restriction":false},"short-container-title":["PLoS Pathog"],"abstract":"<jats:p>Malaria is a hazardous disease caused by<jats:italic>Plasmodium<\/jats:italic>parasites and often results in lethal complications, including malaria-associated acute respiratory distress syndrome (MA-ARDS). Parasite sequestration in the microvasculature is often observed, but its role in malaria pathogenesis and complications is still incompletely understood. We used skeleton binding protein-1 (SBP-1) KO parasites to study the role of sequestration in experimental MA-ARDS. The sequestration-deficiency of these SBP-1 KO parasites was confirmed with bioluminescence imaging and by measuring parasite accumulation in the lungs with RT-qPCR. The SBP-1 KO parasites induced similar lung pathology in the early stage of experimental MA-ARDS compared to wildtype (WT) parasites. Strikingly, the lung pathology resolved subsequently in more than 60% of the SBP-1 KO infected mice, resulting in prolonged survival despite the continuous presence of the parasite. This spontaneous disease resolution was associated with decreased inflammatory cytokine expression measured by RT-qPCR and lower expression of cytotoxic markers in pathogenic CD8<jats:sup>+<\/jats:sup>T cells in the lungs of SBP-1 KO infected mice. These data suggest that SBP-1-mediated parasite sequestration and subsequent high parasite load are not essential for the development of experimental MA-ARDS but inhibit the resolution of the disease.<\/jats:p>","DOI":"10.1371\/journal.ppat.1010114","type":"journal-article","created":{"date-parts":[[2021,11,29]],"date-time":"2021-11-29T18:58:05Z","timestamp":1638212285000},"page":"e1010114","update-policy":"https:\/\/doi.org\/10.1371\/journal.ppat.corrections_policy","source":"Crossref","is-referenced-by-count":13,"title":["Skeleton binding protein-1-mediated parasite sequestration inhibits spontaneous resolution of malaria-associated acute respiratory distress syndrome"],"prefix":"10.1371","volume":"17","author":[{"given":"Hendrik","family":"Possemiers","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Thao-Thy","family":"Pham","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marion","family":"Coens","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2706-6259","authenticated-orcid":true,"given":"Emilie","family":"Pollenus","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sofie","family":"Knoops","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sam","family":"Noppen","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6355-3129","authenticated-orcid":true,"given":"Leen","family":"Vandermosten","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0817-4294","authenticated-orcid":true,"given":"Sigrid","family":"D\u2019haese","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5375-8403","authenticated-orcid":true,"given":"Luna","family":"Dillemans","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5431-1715","authenticated-orcid":true,"given":"Fran","family":"Prenen","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3256-5850","authenticated-orcid":true,"given":"Dominique","family":"Schols","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Blandine","family":"Franke-Fayard","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7334-9145","authenticated-orcid":true,"given":"Philippe E.","family":"Van den Steen","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"340","published-online":{"date-parts":[[2021,11,29]]},"reference":[{"key":"ppat.1010114.ref001","volume-title":"WHO World Malaria Report 2020","author":"World Health Organization","year":"2020"},{"issue":"7","key":"ppat.1010114.ref002","doi-asserted-by":"crossref","first-page":"346","DOI":"10.1016\/j.pt.2013.04.006","article-title":"Pathogenesis of malaria-associated acute respiratory distress syndrome","volume":"29","author":"PE Van den Steen","year":"2013","journal-title":"Trends Parasitol"},{"issue":"9","key":"ppat.1010114.ref003","doi-asserted-by":"crossref","first-page":"957","DOI":"10.1164\/rccm.200905-0786OC","article-title":"Immunopathology and dexamethasone therapy in a new model for malaria-associated acute respiratory distress syndrome","volume":"181","author":"PE Van den Steen","year":"2010","journal-title":"Am J Respir Crit Care Med"},{"issue":"4","key":"ppat.1010114.ref004","first-page":"345","article-title":"An ultrastructural study of the brain in fatal Plasmodium falciparum malaria","volume":"69","author":"E Pongponratn","year":"2003","journal-title":"Trop Med"},{"issue":"2","key":"ppat.1010114.ref005","doi-asserted-by":"crossref","first-page":"143","DOI":"10.1038\/nm986","article-title":"Differentiating the pathologies of cerebral malaria by postmortem parasite counts","volume":"10","author":"TE Taylor","year":"2004","journal-title":"Nat Med"},{"key":"ppat.1010114.ref006","doi-asserted-by":"crossref","first-page":"104","DOI":"10.3389\/fcimb.2014.00104","article-title":"The systemic pathology of cerebral malaria in African children","volume":"4","author":"DA Milner","year":"2014","journal-title":"Front Cell Infect Microbiol"},{"issue":"3","key":"ppat.1010114.ref007","first-page":"385","article-title":"Human cerebral malaria. 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