{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T15:23:32Z","timestamp":1790695412988,"version":"4.1.0"},"reference-count":77,"publisher":"Public Library of Science (PLoS)","issue":"2","license":[{"start":{"date-parts":[[2022,2,8]],"date-time":"2022-02-08T00:00:00Z","timestamp":1644278400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/publicdomain\/zero\/1.0\/"}],"funder":[{"DOI":"10.13039\/501100001659","name":"Deutsche Forschungsgemeinschaft","doi-asserted-by":"publisher","award":["LU1357\/5-1"],"award-info":[{"award-number":["LU1357\/5-1"]}],"id":[{"id":"10.13039\/501100001659","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100002347","name":"Bundesministerium f\u00fcr Bildung und Forschung","doi-asserted-by":"publisher","award":["01KI1726A"],"award-info":[{"award-number":["01KI1726A"]}],"id":[{"id":"10.13039\/501100002347","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["www.plospathogens.org"],"crossmark-restriction":false},"short-container-title":["PLoS Pathog"],"abstract":"<jats:p>\n                    Inhibition of host cell apoptosis is crucial for survival and replication of several intracellular bacterial pathogens. To interfere with apoptotic pathways, some pathogens use specialized secretion systems to inject bacterial effector proteins into the host cell cytosol. One of these pathogens is the obligate intracellular bacterium\n                    <jats:italic>Coxiella burnetii<\/jats:italic>\n                    , the etiological agent of the zoonotic disease Q fever. In this study, we analyzed the molecular activity of the anti-apoptotic T4SS effector protein AnkG (CBU0781) to understand how\n                    <jats:italic>C<\/jats:italic>\n                    .\n                    <jats:italic>burnetii<\/jats:italic>\n                    manipulates host cell viability. We demonstrate by co- and RNA-immunoprecipitation that AnkG binds to the host cell DExD box RNA helicase 21 (DDX21) as well as to the host cell 7SK small nuclear ribonucleoprotein (7SK snRNP) complex, an important regulator of the positive transcription elongation factor b (P-TEFb). The co-immunoprecipitation of AnkG with DDX21 is probably mediated by salt bridges and is independent of AnkG-7SK snRNP binding, and\n                    <jats:italic>vice versa<\/jats:italic>\n                    . It is known that DDX21 facilitates the release of P-TEFb from the 7SK snRNP complex. Consistent with the documented function of released P-TEFb in RNA Pol II pause release, RNA sequencing experiments confirmed AnkG-mediated transcriptional reprogramming and showed that expression of genes involved in apoptosis, trafficking, and transcription are influenced by AnkG. Importantly, DDX21 and P-TEFb are both essential for AnkG-mediated inhibition of host cell apoptosis, emphasizing the significance of the interaction of AnkG with both, the DDX21 protein and the 7SK RNA. In line with a critical function of AnkG in pathogenesis, the AnkG deletion\n                    <jats:italic>C<\/jats:italic>\n                    .\n                    <jats:italic>burnetii<\/jats:italic>\n                    strain was severely affected in its ability to inhibit host cell apoptosis and to generate a replicative\n                    <jats:italic>C<\/jats:italic>\n                    .\n                    <jats:italic>burnetii<\/jats:italic>\n                    -containing vacuole. In conclusion, the interference with the activity of regulatory host cell RNAs mediated by a bacterial effector protein represent a novel mechanism through which\n                    <jats:italic>C<\/jats:italic>\n                    .\n                    <jats:italic>burnetii<\/jats:italic>\n                    modulates host cell transcription, thereby enhancing permissiveness to bacterial infection.\n                  <\/jats:p>","DOI":"10.1371\/journal.ppat.1010266","type":"journal-article","created":{"date-parts":[[2022,2,8]],"date-time":"2022-02-08T13:32:12Z","timestamp":1644327132000},"page":"e1010266","update-policy":"https:\/\/doi.org\/10.1371\/journal.ppat.corrections_policy","source":"Crossref","is-referenced-by-count":30,"title":["The Coxiella burnetii T4SS effector protein AnkG hijacks the 7SK small nuclear ribonucleoprotein complex for reprogramming host cell transcription"],"prefix":"10.1371","volume":"18","author":[{"given":"Arne","family":"Cordsmeier","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4924-2604","authenticated-orcid":true,"given":"Sven","family":"Rinkel","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8432-6045","authenticated-orcid":true,"given":"Myriam","family":"Jeninga","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3810-4457","authenticated-orcid":true,"given":"Jan","family":"Schulze-Luehrmann","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8364-9918","authenticated-orcid":true,"given":"Martha","family":"\u00d6lke","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Benedikt","family":"Schmid","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1786-0563","authenticated-orcid":true,"given":"Daniele","family":"Hasler","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2098-9923","authenticated-orcid":true,"given":"Gunter","family":"Meister","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1058-5746","authenticated-orcid":true,"given":"Georg","family":"H\u00e4cker","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1837-2756","authenticated-orcid":true,"given":"Michaela","family":"Petter","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Paul A.","family":"Beare","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1224-0880","authenticated-orcid":true,"given":"Anja","family":"L\u00fchrmann","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"340","published-online":{"date-parts":[[2022,2,8]]},"reference":[{"issue":"5","key":"ppat.1010266.ref001","doi-asserted-by":"crossref","first-page":"945","DOI":"10.1093\/ije\/22.5.945","article-title":"Q fever is absent from New Zealand","volume":"22","author":"F Hilbink","year":"1993","journal-title":"Int J Epidemiol"},{"issue":"4","key":"ppat.1010266.ref002","doi-asserted-by":"crossref","first-page":"518","DOI":"10.1128\/CMR.12.4.518","article-title":"Q fever","volume":"12","author":"M Maurin","year":"1999","journal-title":"Clin Microbiol Rev"},{"issue":"1\u20132","key":"ppat.1010266.ref003","doi-asserted-by":"crossref","first-page":"119","DOI":"10.1016\/j.vetmic.2015.07.011","article-title":"Coxiella burnetii infections in sheep or goats: an opinionated review","volume":"181","author":"R Van den Brom","year":"2015","journal-title":"Vet Microbiol"},{"key":"ppat.1010266.ref004","doi-asserted-by":"crossref","first-page":"488","DOI":"10.1186\/1471-2334-13-488","article-title":"Human dose response relation for airborne exposure to Coxiella burnetii.","volume":"13","author":"RJ Brooke","year":"2013","journal-title":"BMC Infect Dis"},{"issue":"1","key":"ppat.1010266.ref005","doi-asserted-by":"crossref","first-page":"115","DOI":"10.1128\/CMR.00045-16","article-title":"From Q Fever to Coxiella burnetii Infection: a Paradigm Change","volume":"30","author":"C Eldin","year":"2017","journal-title":"Clin Microbiol 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Eckart","year":"2014","journal-title":"Infect Immun"},{"issue":"44","key":"ppat.1010266.ref015","doi-asserted-by":"crossref","first-page":"18997","DOI":"10.1073\/pnas.1004380107","article-title":"Inhibition of pathogen-induced apoptosis by a Coxiella burnetii type IV effector protein","volume":"107","author":"A L\u00fchrmann","year":"2010","journal-title":"Proc Natl Acad Sci U S A"},{"issue":"1","key":"ppat.1010266.ref016","doi-asserted-by":"crossref","DOI":"10.1111\/cmi.12634","article-title":"Nuclear trafficking of the anti-apoptotic Coxiella burnetii effector protein AnkG requires binding to p32 and Importin-alpha1","volume":"19","author":"W Sch\u00e4fer","year":"2017","journal-title":"Cell Microbiol"},{"issue":"1","key":"ppat.1010266.ref017","doi-asserted-by":"crossref","first-page":"15396","DOI":"10.1038\/s41598-020-72340-9","article-title":"The anti-apoptotic Coxiella burnetii effector protein AnkG is a strain specific virulence factor","volume":"10","author":"W Sch\u00e4fer","year":"2020","journal-title":"Scientific Reports."},{"issue":"4","key":"ppat.1010266.ref018","first-page":"619","article-title":"Defying Death\u2014How Coxiella burnetii Copes with Intentional Host Cell Suicide","volume":"92","author":"A Cordsmeier","year":"2019","journal-title":"Yale J Biol Med"},{"issue":"1","key":"ppat.1010266.ref019","doi-asserted-by":"crossref","first-page":"e1009184","DOI":"10.1371\/journal.ppat.1009184","article-title":"Bacterial nucleomodulins: A coevolutionary adaptation to the eukaryotic command center","volume":"17","author":"HE Hanford","year":"2021","journal-title":"PLoS Pathog"},{"issue":"4","key":"ppat.1010266.ref020","doi-asserted-by":"crossref","DOI":"10.3390\/toxins12040220","article-title":"Bacterial Factors Targeting the Nucleus: The Growing Family of Nucleomodulins.","volume":"12","author":"H Bierne","year":"2020","journal-title":"Toxins 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Cell"},{"issue":"5","key":"ppat.1010266.ref027","doi-asserted-by":"crossref","first-page":"1014","DOI":"10.1016\/j.molcel.2020.01.001","article-title":"The Alazami Syndrome-Associated Protein LARP7 Guides U6 Small Nuclear RNA Modification and Contributes to Splicing Robustness","volume":"77","author":"D Hasler","year":"2020","journal-title":"Mol Cell"},{"key":"ppat.1010266.ref028","first-page":"1","article-title":"Stabilize and connect: the role of LARP7 in nuclear non-coding RNA metabolism","volume":"2020","author":"D Hasler","year":"2020","journal-title":"RNA Biol"},{"issue":"1","key":"ppat.1010266.ref029","doi-asserted-by":"crossref","first-page":"12516","DOI":"10.1038\/s41598-019-49032-0","article-title":"de novo MEPCE nonsense variant associated with a neurodevelopmental disorder causes disintegration of 7SK snRNP and enhanced RNA polymerase II activation","volume":"9","author":"PE Schneeberger","year":"2019","journal-title":"Sci 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Pan","year":"2008","journal-title":"Science"},{"issue":"23","key":"ppat.1010266.ref042","doi-asserted-by":"crossref","DOI":"10.1128\/JB.00431-19","article-title":"Dependency of Coxiella burnetii Type 4B Secretion on the Chaperone IcmS","volume":"201","author":"CL Larson","year":"2019","journal-title":"J Bacteriol"},{"issue":"13","key":"ppat.1010266.ref043","doi-asserted-by":"crossref","first-page":"4232","DOI":"10.1128\/JB.01656-08","article-title":"The Coxiella burnetii ankyrin repeat domain-containing protein family is heterogeneous, with C-terminal truncations that influence Dot\/Icm-mediated secretion","volume":"191","author":"DE Voth","year":"2009","journal-title":"J Bacteriol"},{"issue":"8","key":"ppat.1010266.ref044","doi-asserted-by":"crossref","first-page":"3465","DOI":"10.1128\/IAI.00406-10","article-title":"Coxiella burnetii phase I and II variants replicate with similar kinetics in degradative phagolysosome-like compartments of human macrophages","volume":"78","author":"D Howe","year":"2010","journal-title":"Infect Immun"},{"key":"ppat.1010266.ref045","doi-asserted-by":"crossref","first-page":"88","DOI":"10.1016\/j.mib.2017.03.001","article-title":"Modulation of host cell apoptotic pathways by intracellular pathogens","volume":"35","author":"A Friedrich","year":"2017","journal-title":"Curr Opin Microbiol"},{"issue":"24","key":"ppat.1010266.ref046","doi-asserted-by":"crossref","first-page":"13708","DOI":"10.1073\/pnas.1914892117","article-title":"Modulation of innate immune signaling by a Coxiella burnetii eukaryotic-like effector protein","volume":"117","author":"M Burette","year":"2020","journal-title":"Proc Natl Acad Sci U S A"},{"issue":"50","key":"ppat.1010266.ref047","doi-asserted-by":"crossref","first-page":"21755","DOI":"10.1073\/pnas.1010485107","article-title":"Large-scale identification and translocation of type IV secretion substrates by Coxiella burnetii","volume":"107","author":"C Chen","year":"2010","journal-title":"Proc Natl Acad Sci U S 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Bisle","year":"2016","journal-title":"Virulence"},{"issue":"5","key":"ppat.1010266.ref051","doi-asserted-by":"crossref","first-page":"336","DOI":"10.1016\/j.micinf.2016.01.003","article-title":"Modulation of the host transcriptome by Coxiella burnetii nuclear effector Cbu1314","volume":"18","author":"MM Weber","year":"2016","journal-title":"Microbes Infect"},{"issue":"11","key":"ppat.1010266.ref052","doi-asserted-by":"crossref","first-page":"2644","DOI":"10.1111\/j.1462-5822.2007.00985.x","article-title":"Anaplasma phagocytophilum AnkA secreted by type IV secretion system is tyrosine phosphorylated by Abl-1 to facilitate infection","volume":"9","author":"M Lin","year":"2007","journal-title":"Cell Microbiol"},{"issue":"8","key":"ppat.1010266.ref053","doi-asserted-by":"crossref","first-page":"743","DOI":"10.1111\/j.1462-5822.2004.00400.x","article-title":"Anaplasma phagocytophilum AnkA binds to granulocyte DNA and nuclear proteins","volume":"6","author":"J Park","year":"2004","journal-title":"Cell Microbiol"},{"issue":"6","key":"ppat.1010266.ref054","doi-asserted-by":"crossref","first-page":"2385","DOI":"10.1128\/IAI.00023-09","article-title":"Silencing of host cell CYBB gene expression by the nuclear effector AnkA of the intracellular pathogen Anaplasma phagocytophilum","volume":"77","author":"JC Garcia-Garcia","year":"2009","journal-title":"Infect Immun"},{"issue":"11","key":"ppat.1010266.ref055","doi-asserted-by":"crossref","first-page":"1640","DOI":"10.1111\/cmi.12461","article-title":"Chromatin-bound bacterial effector ankyrin A recruits histone deacetylase 1 and modifies host gene expression","volume":"17","author":"KE Rennoll-Bankert","year":"2015","journal-title":"Cell Microbiol"},{"issue":"7","key":"ppat.1010266.ref056","doi-asserted-by":"crossref","first-page":"e1000995","DOI":"10.1371\/journal.ppat.1000995","article-title":"Histone methylation by NUE, a novel nuclear effector of the intracellular pathogen Chlamydia 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Trapnell","year":"2010","journal-title":"Nat Biotechnol"},{"issue":"1","key":"ppat.1010266.ref072","doi-asserted-by":"crossref","first-page":"46","DOI":"10.1038\/nbt.2450","article-title":"Differential analysis of gene regulation at transcript resolution with RNA-seq","volume":"31","author":"C Trapnell","year":"2013","journal-title":"Nat Biotechnol"},{"issue":"W1","key":"ppat.1010266.ref073","doi-asserted-by":"crossref","first-page":"W160","DOI":"10.1093\/nar\/gkw257","article-title":"deepTools2: a next generation web server for deep-sequencing data analysis","volume":"44","author":"F Ramirez","year":"2016","journal-title":"Nucleic Acids Res"},{"issue":"3","key":"ppat.1010266.ref074","doi-asserted-by":"crossref","first-page":"e1006922","DOI":"10.1371\/journal.ppat.1006922","article-title":"Genetic mechanisms of Coxiella burnetii lipopolysaccharide phase variation","volume":"14","author":"PA Beare","year":"2018","journal-title":"PLoS 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