{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,31]],"date-time":"2025-12-31T10:58:52Z","timestamp":1767178732918,"version":"build-2238731810"},"update-to":[{"DOI":"10.1371\/journal.ppat.1010300","type":"new_version","label":"New version","source":"publisher","updated":{"date-parts":[[2022,2,22]],"date-time":"2022-02-22T00:00:00Z","timestamp":1645488000000}}],"reference-count":91,"publisher":"Public Library of Science (PLoS)","issue":"2","license":[{"start":{"date-parts":[[2022,2,9]],"date-time":"2022-02-09T00:00:00Z","timestamp":1644364800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100000268","name":"biotechnology and biological sciences research council","doi-asserted-by":"publisher","award":["BBSRC project grant BB\/R016437\/1"],"award-info":[{"award-number":["BBSRC project grant BB\/R016437\/1"]}],"id":[{"id":"10.13039\/501100000268","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100000268","name":"biotechnology and biological sciences research council","doi-asserted-by":"publisher","award":["BBSRC project grant BB\/R016437\/1"],"award-info":[{"award-number":["BBSRC project grant BB\/R016437\/1"]}],"id":[{"id":"10.13039\/501100000268","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100000268","name":"biotechnology and biological sciences research council","doi-asserted-by":"publisher","award":["BBSRC project grant BB\/R010188\/1"],"award-info":[{"award-number":["BBSRC project grant BB\/R010188\/1"]}],"id":[{"id":"10.13039\/501100000268","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100000288","name":"Royal Society","doi-asserted-by":"crossref","award":["URF\\R\\201024"],"award-info":[{"award-number":["URF\\R\\201024"]}],"id":[{"id":"10.13039\/501100000288","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":["www.plospathogens.org"],"crossmark-restriction":false},"short-container-title":["PLoS Pathog"],"abstract":"<jats:p>\n                    Genetic exchange among disease-causing micro-organisms can generate progeny that combine different pathogenic traits. Though sexual reproduction has been described in trypanosomes, its impact on the epidemiology of Human African Trypanosomiasis (HAT) remains controversial. However, human infective and non-human infective strains of\n                    <jats:italic>Trypanosoma brucei<\/jats:italic>\n                    circulate in the same transmission cycles in HAT endemic areas in subsaharan Africa, providing the opportunity for mating during the developmental cycle in the tsetse fly vector. Here we investigated inheritance among progeny from a laboratory cross of\n                    <jats:italic>T<\/jats:italic>\n                    .\n                    <jats:italic>brucei<\/jats:italic>\n                    and then applied these insights to genomic analysis of field-collected isolates to identify signatures of past genetic exchange. Genomes of two parental and four hybrid progeny clones with a range of DNA contents were assembled and analysed by k-mer and single nucleotide polymorphism (SNP) frequencies to determine heterozygosity and chromosomal inheritance. Variant surface glycoprotein (VSG) genes and kinetoplast (mitochondrial) DNA maxi- and minicircles were extracted from each genome to examine how each of these components was inherited in the hybrid progeny. The same bioinformatic approaches were applied to an additional 37 genomes representing the diversity of\n                    <jats:italic>T<\/jats:italic>\n                    .\n                    <jats:italic>brucei<\/jats:italic>\n                    in subsaharan Africa and\n                    <jats:italic>T<\/jats:italic>\n                    .\n                    <jats:italic>evansi<\/jats:italic>\n                    . SNP analysis provided evidence of crossover events affecting all 11 pairs of megabase chromosomes and demonstrated that polyploid hybrids were formed post-meiotically and not by fusion of the parental diploid cells.\n                    <jats:italic>VSG<\/jats:italic>\n                    s and kinetoplast DNA minicircles were inherited biparentally, with approximately equal numbers from each parent, whereas maxicircles were inherited uniparentally. Extrapolation of these findings to field isolates allowed us to distinguish clonal descent from hybridization by comparing maxicircle genotype to\n                    <jats:italic>VSG<\/jats:italic>\n                    and minicircle repertoires. Discordance between maxicircle genotype and\n                    <jats:italic>VSG<\/jats:italic>\n                    and minicircle repertoires indicated inter-lineage hybridization. Significantly, some of the hybridization events we identified involved human infective and non-human infective trypanosomes circulating in the same geographic areas.\n                  <\/jats:p>","DOI":"10.1371\/journal.ppat.1010300","type":"journal-article","created":{"date-parts":[[2022,2,9]],"date-time":"2022-02-09T13:39:20Z","timestamp":1644413960000},"page":"e1010300","update-policy":"https:\/\/doi.org\/10.1371\/journal.ppat.corrections_policy","source":"Crossref","is-referenced-by-count":10,"title":["Signatures of hybridization in Trypanosoma brucei"],"prefix":"10.1371","volume":"18","author":[{"given":"Christopher","family":"Kay","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lori","family":"Peacock","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1072-0223","authenticated-orcid":true,"given":"Tom A.","family":"Williams","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4589-723X","authenticated-orcid":true,"given":"Wendy","family":"Gibson","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"340","published-online":{"date-parts":[[2022,2,9]]},"reference":[{"key":"ppat.1010300.ref001","doi-asserted-by":"crossref","first-page":"173","DOI":"10.1038\/322173a0","article-title":"Hybrid formation between African trypanosomes during cyclical transmission","volume":"322","author":"L Jenni","year":"1986","journal-title":"Nature"},{"key":"ppat.1010300.ref002","doi-asserted-by":"crossref","first-page":"4","DOI":"10.1186\/1756-3305-1-4","article-title":"The use of yellow fluorescent hybrids to indicate mating in Trypanosoma brucei.","volume":"1","author":"W Gibson","year":"2008","journal-title":"Parasit 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