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Here, we investigated the neuroinvasion of the mouse spinal cord by<jats:italic>Trichobilharzia regenti<\/jats:italic>(Schistosomatidae). Active migration of<jats:italic>T<\/jats:italic>.<jats:italic>regenti<\/jats:italic>schistosomula through the mouse spinal cord induced motor deficits in hindlimbs but did not affect the general locomotion or working memory. Histological examination of the infected spinal cord revealed eosinophilic meningomyelitis with eosinophil-rich infiltrates entrapping the schistosomula. Flow cytometry and transcriptomic analysis of the spinal cord confirmed massive activation of the host immune response. Of note, we recorded striking upregulation of the major histocompatibility complex II pathway and M2-associated markers, such as arginase or chitinase-like 3. Arginase also dominated the proteins found in the microdissected tissue from the close vicinity of the migrating schistosomula, which unselectively fed on the host nervous tissue. Next, we evaluated the pathological sequelae of<jats:italic>T<\/jats:italic>.<jats:italic>regenti<\/jats:italic>neuroinvasion. While no demyelination or blood-brain barrier alterations were noticed, our transcriptomic data revealed a remarkable disruption of neurophysiological functions not yet recorded in helminth neuroinfections. We also detected DNA fragmentation at the host-schistosomulum interface, but schistosomula antigens did not affect the viability of neurons and glial cells<jats:italic>in vitro<\/jats:italic>. Collectively, altered locomotion, significant disruption of neurophysiological functions, and strong M2 polarization were the most prominent features of<jats:italic>T<\/jats:italic>.<jats:italic>regenti<\/jats:italic>neuroinvasion, making it a promising candidate for further neuroinfection research. Indeed, understanding the diversity of pathogen-related neuroinflammatory processes is a prerequisite for developing better protective measures, treatment strategies, and diagnostic tools.<\/jats:p>","DOI":"10.1371\/journal.ppat.1010302","type":"journal-article","created":{"date-parts":[[2022,2,4]],"date-time":"2022-02-04T18:38:58Z","timestamp":1643999938000},"page":"e1010302","update-policy":"https:\/\/doi.org\/10.1371\/journal.ppat.corrections_policy","source":"Crossref","is-referenced-by-count":15,"title":["Mechanisms of the host immune response and helminth-induced pathology during Trichobilharzia regenti (Schistosomatidae) neuroinvasion in mice"],"prefix":"10.1371","volume":"18","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-6310-4099","authenticated-orcid":true,"given":"Tom\u00e1\u0161","family":"Mach\u00e1\u010dek","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0035-949X","authenticated-orcid":true,"given":"Roman","family":"Leontovy\u010d","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5746-9657","authenticated-orcid":true,"given":"Barbora","family":"\u0160m\u00eddov\u00e1","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7570-0771","authenticated-orcid":true,"given":"Martin","family":"Majer","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6745-3803","authenticated-orcid":true,"given":"Old\u0159ich","family":"Vondr\u00e1\u010dek","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7029-7936","authenticated-orcid":true,"given":"Iveta","family":"Vojt\u011bchov\u00e1","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3793-8364","authenticated-orcid":true,"given":"Tom\u00e1\u0161","family":"Petr\u00e1sek","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9820-5044","authenticated-orcid":true,"given":"Petr","family":"Hor\u00e1k","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"340","published-online":{"date-parts":[[2022,2,4]]},"reference":[{"key":"ppat.1010302.ref001","doi-asserted-by":"crossref","first-page":"257","DOI":"10.1017\/S0022149X12000600","article-title":"Parasitoses 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Holland C","year":"2013","journal-title":"J Exp Biol"},{"key":"ppat.1010302.ref011","doi-asserted-by":"crossref","first-page":"46","DOI":"10.1016\/j.actatropica.2014.10.002","article-title":"Central nervous system manifestations of Angiostrongylus cantonensis infection","volume":"141","author":"YC Martins","year":"2015","journal-title":"Acta Trop"},{"key":"ppat.1010302.ref012","doi-asserted-by":"crossref","first-page":"553","DOI":"10.1017\/S0031182018001932","article-title":"Model systems for investigating disease processes in neurocysticercosis","volume":"146","author":"A de Lange","year":"2019","journal-title":"Parasitology"},{"key":"ppat.1010302.ref013","doi-asserted-by":"crossref","unstructured":"Strube C, Waindok P, Raulf M-K, Springer A. Toxocara-induced neural larva migrans (neurotoxocarosis) in rodent model hosts. 1st ed. In: Bowman DD, editor. Toxocara and Toxocariasis. 1st ed. 2020. pp. 189\u2013218. doi: 10.1016\/bs.apar.2020.01.006","DOI":"10.1016\/bs.apar.2020.01.006"},{"key":"ppat.1010302.ref014","doi-asserted-by":"crossref","first-page":"599","DOI":"10.1590\/S0074-02762002000400028","article-title":",Experimental neuroschistosomiasis: Inadequacy of the murine model","volume":"97","author":"LM Silva","year":"2002","journal-title":"Mem Inst Oswaldo Cruz"},{"key":"ppat.1010302.ref015","doi-asserted-by":"crossref","first-page":"251","DOI":"10.1590\/0037-8682-0083-2013","article-title":"Brain schistosomiasis in mice experimentally infected with Schistosoma mansoni","volume":"47","author":"JR Lambertucci","year":"2014","journal-title":"Rev Soc Bras Med Trop"},{"key":"ppat.1010302.ref016","doi-asserted-by":"crossref","first-page":"1088","DOI":"10.1186\/s12879-019-4725-5","article-title":"Identification and characterization of microglia\/macrophages in the granuloma microenvironment of encephalic schistosomiasis 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Hor\u00e1k","year":"1999","journal-title":"Parasitology"},{"key":"ppat.1010302.ref020","doi-asserted-by":"crossref","first-page":"137","DOI":"10.1079\/JOH2002113","article-title":"Neurotropic behaviour of Trichobilharzia regenti in ducks and mice","volume":"76","author":"K Hr\u00e1dkov\u00e1","year":"2002","journal-title":"J Helminthol"},{"key":"ppat.1010302.ref021","doi-asserted-by":"crossref","first-page":"8","DOI":"10.1007\/s00436-004-1079-7","article-title":"The severity of mouse pathologies caused by the bird schistosome Trichobilharzia regenti in relation to host immune status","volume":"93","author":"P Kou\u0159ilov\u00e1","year":"2004","journal-title":"Parasitol Res"},{"key":"ppat.1010302.ref022","doi-asserted-by":"crossref","first-page":"328","DOI":"10.1016\/j.exppara.2011.04.006","article-title":"Trichobilharzia regenti: Host immune response in the pathogenesis of neuroinfection in mice","volume":"128","author":"L Lichtenbergov\u00e1","year":"2011","journal-title":"Exp Parasitol"},{"key":"ppat.1010302.ref023","doi-asserted-by":"crossref","first-page":"1374","DOI":"10.1038\/s41598-018-37669-2","article-title":"Molecular evidence for distinct modes of nutrient acquisition between visceral and neurotropic schistosomes of birds.","volume":"9","author":"R Leontovy\u010d","year":"2019","journal-title":"Sci Rep"},{"key":"ppat.1010302.ref024","doi-asserted-by":"crossref","first-page":"644","DOI":"10.1007\/s004360100431","article-title":"Histopathology of CNS and nasal infections caused by Trichobilharzia regenti in vertebrates","volume":"87","author":"L Kol\u00e1\u0159ov\u00e1","year":"2001","journal-title":"Parasitol Res"},{"key":"ppat.1010302.ref025","doi-asserted-by":"crossref","first-page":"579","DOI":"10.1186\/s13071-016-1869-7","article-title":"Nitric oxide and cytokine production by glial cells exposed in vitro to neuropathogenic schistosome Trichobilharzia regenti","volume":"9","author":"T Mach\u00e1\u010dek","year":"2016","journal-title":"Parasit 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dermatitis","volume":"30","author":"L Lichtenbergov\u00e1","year":"2008","journal-title":"Parasite Immunol"},{"key":"ppat.1010302.ref029","doi-asserted-by":"crossref","first-page":"e12710","DOI":"10.1111\/pim.12710","article-title":"The peripheral immune response of mice infected with a neuropathogenic schistosome","volume":"42","author":"M Majer","year":"2020","journal-title":"Parasite Immunol"},{"key":"ppat.1010302.ref030","doi-asserted-by":"crossref","first-page":"1209","DOI":"10.1016\/0092-8674(94)90012-4","article-title":"KIF1B, a novel microtubule plus end-directed monomeric motor protein for transport of mitochondria","volume":"79","author":"M Nangaku","year":"1994","journal-title":"Cell"},{"key":"ppat.1010302.ref031","doi-asserted-by":"crossref","first-page":"769","DOI":"10.1016\/0092-8674(95)90538-3","article-title":"The neuron-specific kinesin superfamily protein KIF1A is a uniqye monomeric motor for anterograde axonal transport of synaptic vesicle precursors","volume":"81","author":"Y Okada","year":"1995","journal-title":"Cell"},{"key":"ppat.1010302.ref032","doi-asserted-by":"crossref","first-page":"3072","DOI":"10.1038\/s41598-018-21470-2","article-title":"Cytoskeleton stability is essential for the integrity of the cerebellum and its motor- and affective-related behaviors.","volume":"8","author":"R Mu\u00f1oz-Casta\u00f1eda","year":"2018","journal-title":"Sci Rep"},{"key":"ppat.1010302.ref033","doi-asserted-by":"crossref","first-page":"453","DOI":"10.1083\/jcb.131.2.453","article-title":"Rapid, widespread, and longlasting induction of nestin contributes to the generation of glial scar tissue after CNS injury","volume":"131","author":"J Fris\u00e9n","year":"1995","journal-title":"J Cell Biol"},{"key":"ppat.1010302.ref034","doi-asserted-by":"crossref","first-page":"3879","DOI":"10.1038\/s41467-019-11707-7","article-title":"Moving beyond the glial scar for spinal cord repair.","volume":"10","author":"EJ Bradbury","year":"2019","journal-title":"Nat Commun"},{"key":"ppat.1010302.ref035","doi-asserted-by":"crossref","first-page":"219","DOI":"10.1042\/AN20100016","article-title":"Increased Plp1 gene expression leads to massive microglial cell activation and inflammation throughout the brain","volume":"2","author":"CL Tatar","year":"2010","journal-title":"ASN Neuro"},{"key":"ppat.1010302.ref036","doi-asserted-by":"crossref","first-page":"482","DOI":"10.1084\/jem.20182031","article-title":"Interferon-induced guanylate-binding proteins: Guardians of host defense in health and disease","volume":"216","author":"K Tretina","year":"2019","journal-title":"J Exp Med"},{"key":"ppat.1010302.ref037","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/S0169-328X(98)00040-0","article-title":"Microglia-specific localisation of a novel calcium binding protein, Iba1","volume":"57","author":"D Ito","year":"1998","journal-title":"Brain Res Mol Brain Res"},{"key":"ppat.1010302.ref038","doi-asserted-by":"crossref","first-page":"844","DOI":"10.1046\/j.1471-4159.2003.02213.x","article-title":"Microglia\/macrophage-specific protein Iba1 binds to fimbrin and enhances its actin-bundling activity","volume":"88","author":"K Ohsawa","year":"2004","journal-title":"J Neurochem"},{"key":"ppat.1010302.ref039","doi-asserted-by":"crossref","first-page":"2650","DOI":"10.4049\/jimmunol.180.4.2650","article-title":"Regulation of alternative macrophage activation by galectin-3","volume":"180","author":"AC MacKinnon","year":"2008","journal-title":"J Immunol"},{"key":"ppat.1010302.ref040","doi-asserted-by":"crossref","first-page":"250","DOI":"10.1038\/s41419-018-0288-4","article-title":"Interleukin 4 modulates microglia homeostasis and attenuates the early slowly progressive phase of amyotrophic lateral sclerosis","volume":"9","author":"C Rossi","year":"2018","journal-title":"Cell Death Dis"},{"key":"ppat.1010302.ref041","doi-asserted-by":"crossref","first-page":"62","DOI":"10.1016\/j.micron.2016.01.009","article-title":"Trichobilharzia regenti (Schistosomatidae): 3D imaging techniques in characterization of larval migration through the CNS of vertebrates.","volume":"83","author":"J Bulantov\u00e1","year":"2016","journal-title":"Micron"},{"key":"ppat.1010302.ref042","doi-asserted-by":"crossref","first-page":"323","DOI":"10.1016\/S1537-1891(02)00200-8","article-title":"Tight junctions of the blood-brain barrier: development, composition and regulation.","volume":"38","author":"H Wolburg","year":"2002","journal-title":"Vascul Pharmacol"},{"key":"ppat.1010302.ref043","doi-asserted-by":"crossref","first-page":"312","DOI":"10.1038\/s41593-020-00783-4","article-title":"Reactive astrocyte nomenclature, definitions, and future directions","volume":"24","author":"C Escartin","year":"2021","journal-title":"Nat Neurosci"},{"key":"ppat.1010302.ref044","doi-asserted-by":"crossref","first-page":"265","DOI":"10.1002\/jnr.20044","article-title":"Blockade of interleukin-6 receptor suppresses reactive astrogliosis and ameliorates functional recovery in experimental spinal cord injury","volume":"76","author":"S Okada","year":"2004","journal-title":"J Neurosci Res"},{"key":"ppat.1010302.ref045","doi-asserted-by":"crossref","first-page":"388","DOI":"10.1038\/nri3839","article-title":"Perforin and granzymes: function, dysfunction and human pathology","volume":"15","author":"I Voskoboinik","year":"2015","journal-title":"Nat Rev Immunol"},{"key":"ppat.1010302.ref046","doi-asserted-by":"crossref","first-page":"165","DOI":"10.1128\/CMR.00043-14","article-title":"Avian schistosomes and outbreaks of cercarial dermatitis","volume":"28","author":"P Hor\u00e1k","year":"2015","journal-title":"Clin Microbiol 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Jarolim","year":"2000","journal-title":"J Parasitol"},{"key":"ppat.1010302.ref050","doi-asserted-by":"crossref","first-page":"639","DOI":"10.2174\/1566524023361989","article-title":"A comparative review of rodent prefrontal cortex and working memory","volume":"2","author":"M. Jones","year":"2002","journal-title":"Curr Mol Med"},{"key":"ppat.1010302.ref051","doi-asserted-by":"crossref","first-page":"5632","DOI":"10.1073\/pnas.0611554104","article-title":"Kandel ER. Consolidation of learning strategies during spatial working memory task requires protein synthesis in the prefrontal cortex","volume":"104","author":"K Touzani","year":"2007","journal-title":"Proc Natl Acad Sci"},{"key":"ppat.1010302.ref052","doi-asserted-by":"crossref","first-page":"e00316","DOI":"10.1016\/j.heliyon.2017.e00316","article-title":"Pattern of c-Fos expression induced by tail suspension test in the mouse brain","volume":"3","author":"K Hiraoka","year":"2017","journal-title":"Heliyon"},{"key":"ppat.1010302.ref053","doi-asserted-by":"crossref","first-page":"23","DOI":"10.1079\/JOH200027","article-title":"Influence of mouse strain, infective dose and larval burden in the brain on activity","volume":"75","author":"DM Cox","year":"2001","journal-title":"Toxocara-infected mice. 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A frequent cause of seizures, epilepsy, and other neurological morbidity in most of the world","volume":"427","author":"J Bustos","year":"2021","journal-title":"J Neurol Sci"},{"key":"ppat.1010302.ref057","doi-asserted-by":"crossref","first-page":"83","DOI":"10.1016\/j.actatropica.2008.02.010","article-title":"Immune response and pathogenesis of neuroschistosomiasis mansoni","volume":"108","author":"T Ferrari","year":"2008","journal-title":"Acta Trop"},{"key":"ppat.1010302.ref058","doi-asserted-by":"crossref","first-page":"89","DOI":"10.1016\/j.actatropica.2008.04.007","article-title":"Clinical characterization of neuroschistosomiasis due to Schistosoma mansoni and its treatment","volume":"108","author":"T Ferrari","year":"2008","journal-title":"Acta Trop"},{"key":"ppat.1010302.ref059","first-page":"267","article-title":"Immune response from a resource allocation perspective.","volume":"3","author":"WM Rauw","year":"2012","journal-title":"Front 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Springer","year":"2019","journal-title":"Parasitol Res"},{"key":"ppat.1010302.ref069","doi-asserted-by":"crossref","first-page":"431","DOI":"10.1083\/jcb.141.2.431","article-title":"Defect in synaptic vesicle precursor transport and neuronal cell death in KIF1A motor protein\u2013deficient mice","volume":"141","author":"Y Yonekawa","year":"1998","journal-title":"J Cell Biol"},{"key":"ppat.1010302.ref070","doi-asserted-by":"crossref","first-page":"587","DOI":"10.1016\/S0092-8674(01)00363-4","article-title":"Charcot-Marie-Tooth Disease type 2A caused by mutation in a microtubule motor KIF1B\u03b2","volume":"105","author":"C Zhao","year":"2001","journal-title":"Cell"},{"key":"ppat.1010302.ref071","doi-asserted-by":"crossref","first-page":"167","DOI":"10.1016\/j.parint.2005.03.003","article-title":"Trichobilharzia regenti: The developmental differences in natural and abnormal hosts","volume":"54","author":"K Bla\u017eov\u00e1","year":"2005","journal-title":"Parasitol 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Parasitol"},{"key":"ppat.1010302.ref075","doi-asserted-by":"crossref","first-page":"13952","DOI":"10.1074\/jbc.M406037200","article-title":"Identification of a cooperative mechanism involving interleukin-13 and eotaxin-2 in experimental allergic lung inflammation","volume":"280","author":"SM Pope","year":"2005","journal-title":"J Biol Chem"},{"key":"ppat.1010302.ref076","doi-asserted-by":"crossref","first-page":"1279","DOI":"10.1074\/jbc.275.2.1279","article-title":"Identification of a novel eosinophil chemotactic cytokine (ECF-L) as a chitinase family protein.","volume":"275","author":"M Owhashi","year":"2000","journal-title":"J Biol Chem"},{"key":"ppat.1010302.ref077","doi-asserted-by":"crossref","first-page":"30","DOI":"10.1016\/j.jaci.2003.10.050","article-title":"The role of eosinophils in host defense against helminth parasites","volume":"113","author":"AD Klion","year":"2004","journal-title":"J Allergy Clin 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in a dectin-1-dependent manner.","volume":"34","author":"A Ehrens","year":"2021","journal-title":"Cell Rep"},{"key":"ppat.1010302.ref081","doi-asserted-by":"crossref","first-page":"33","DOI":"10.1038\/nri1745","article-title":"Dectin-1: a signalling non-TLR pattern-recognition receptor","volume":"6","author":"GD Brown","year":"2006","journal-title":"Nat Rev Immunol"},{"key":"ppat.1010302.ref082","doi-asserted-by":"crossref","first-page":"942","DOI":"10.1128\/CMR.17.4.942-964.2004","article-title":"Role of microglia in central nervous system infections","volume":"17","author":"RB Rock","year":"2004","journal-title":"Clin Microbiol Rev"},{"key":"ppat.1010302.ref083","doi-asserted-by":"crossref","first-page":"207","DOI":"10.1007\/s00436-012-3127-z","article-title":"Differences in microglia activation between rats-derived cell and mice-derived cell after stimulating by soluble antigen of IV larva from Angiostrongylus cantonensis in vitro","volume":"112","author":"J Wei","year":"2013","journal-title":"Parasitol Res"},{"key":"ppat.1010302.ref084","doi-asserted-by":"crossref","first-page":"3916","DOI":"10.4049\/jimmunol.173.6.3916","article-title":"Microglia initiate central nervous system innate and adaptive immune responses through multiple TLRs","volume":"173","author":"JK Olson","year":"2004","journal-title":"J Immunol"},{"key":"ppat.1010302.ref085","doi-asserted-by":"crossref","first-page":"2586","DOI":"10.1128\/IAI.00920-10","article-title":"Increased disease severity of parasite-infected TLR2\u2212\/\u2212 mice is correlated with decreased central nervous system inflammation and reduced numbers of cells with alternatively activated macrophage phenotypes in a murine model of neurocysticercosis","volume":"79","author":"UM Gundra","year":"2011","journal-title":"Infect Immun"},{"key":"ppat.1010302.ref086","doi-asserted-by":"crossref","first-page":"499","DOI":"10.1038\/ni0602-499","article-title":"Human TLR7 or TLR8 independently confer responsiveness to the antiviral compound R-848","volume":"3","author":"M Jurk","year":"2002","journal-title":"Nat Immunol"},{"key":"ppat.1010302.ref087","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1155\/2013\/456352","article-title":"Parasitic infections: A role for C-type lectins receptors","volume":"2013","author":"A V\u00e1zquez-Mendoza","year":"2013","journal-title":"Biomed Res Int"},{"key":"ppat.1010302.ref088","doi-asserted-by":"crossref","first-page":"9966","DOI":"10.1523\/JNEUROSCI.0337-15.2015","article-title":"Toll-like receptors and dectin-1, a C-type lectin receptor, trigger divergent functions in CNS macrophages","volume":"35","author":"JC Gensel","year":"2015","journal-title":"J Neurosci"},{"key":"ppat.1010302.ref089","doi-asserted-by":"crossref","first-page":"e0173217","DOI":"10.1371\/journal.pone.0173217","article-title":"Changes in surface glycosylation and glycocalyx shedding in Trichobilharzia regenti (Schistosomatidae) during the transformation of cercaria to schistosomulum.","volume":"12","author":"J \u0158imn\u00e1\u010dov\u00e1","year":"2017","journal-title":"PLoS One"},{"key":"ppat.1010302.ref090","doi-asserted-by":"crossref","first-page":"321","DOI":"10.3390\/pathogens10030321","article-title":"Toxocara canis and Toxocara cati somatic and excretory-secretory antigens are recognised by C-type lectin receptors.","volume":"10","author":"M-K Raulf","year":"2021","journal-title":"Pathogens"},{"key":"ppat.1010302.ref091","doi-asserted-by":"crossref","first-page":"e218","DOI":"10.1371\/journal.pntd.0000218","article-title":"Differential release and phagocytosis of tegument glycoconjugates in neurocysticercosis: Implications for immune evasion strategies.","volume":"2","author":"JJI Alvarez","year":"2008","journal-title":"PLoS Negl Trop Dis"},{"key":"ppat.1010302.ref092","doi-asserted-by":"crossref","first-page":"6561","DOI":"10.4049\/jimmunol.174.11.6561","article-title":"Arginase-1 and Ym1 are markers for murine, but not human, alternatively activated myeloid cells","volume":"174","author":"G Raes","year":"2005","journal-title":"J Immunol"},{"key":"ppat.1010302.ref093","doi-asserted-by":"crossref","first-page":"10714","DOI":"10.1523\/JNEUROSCI.1922-07.2007","article-title":"CNS-derived interleukin-4 is essential for the regulation of autoimmune inflammation and induces a state of alternative activation in microglial cells","volume":"27","author":"ED Ponomarev","year":"2007","journal-title":"J Neurosci"},{"key":"ppat.1010302.ref094","doi-asserted-by":"crossref","first-page":"98","DOI":"10.1186\/1742-2094-11-98","article-title":"Neuroinflammation and M2 microglia: the good, the bad, and the inflamed.","volume":"11","author":"JD Cherry","year":"2014","journal-title":"J Neuroinflammation"},{"key":"ppat.1010302.ref095","doi-asserted-by":"crossref","first-page":"26","DOI":"10.1016\/j.jneuroim.2010.09.029","article-title":"STAT6\u2212\/\u2212 mice exhibit decreased cells with alternatively activated macrophage phenotypes and enhanced disease severity in murine neurocysticercosis","volume":"232","author":"BB Mishra","year":"2011","journal-title":"J Neuroimmunol"},{"key":"ppat.1010302.ref096","doi-asserted-by":"crossref","first-page":"860","DOI":"10.1093\/infdis\/jis682","article-title":"Interleukin 33 mediates type 2 immunity and inflammation in the central nervous system of mice infected with Angiostrongylus cantonensis","volume":"207","author":"H Peng","year":"2013","journal-title":"J Infect Dis"},{"key":"ppat.1010302.ref097","doi-asserted-by":"crossref","first-page":"147","DOI":"10.1186\/s12974-019-1537-x","article-title":"Neuroinvasion of Toxocara canis- and T. cati-larvae mediates dynamic changes in brain cytokine and chemokine profile","volume":"16","author":"P Waindok","year":"2019","journal-title":"J Neuroinflammation"},{"key":"ppat.1010302.ref098","doi-asserted-by":"crossref","first-page":"45","DOI":"10.1016\/j.jneuroim.2005.11.020","article-title":"Breakdown of the blood brain barrier and blood\u2013cerebrospinal fluid barrier is associated with differential leukocyte migration in distinct compartments of the CNS during the course of murine NCC","volume":"173","author":"JI Alvarez","year":"2006","journal-title":"J Neuroimmunol"},{"key":"ppat.1010302.ref099","first-page":"1","article-title":"The excretory\/secretory products of fifth stage larval Angiostrongylus cantonensis induces autophagy via the sonic hedgehog pathway in mouse brain astrocytes","volume":"14","author":"KY Chen","year":"2020","journal-title":"PLoS Negl Trop Dis"},{"key":"ppat.1010302.ref100","doi-asserted-by":"crossref","first-page":"13435","DOI":"10.1523\/JNEUROSCI.3257-09.2009","article-title":"Identification of two distinct macrophage subsets with divergent effects causing either neurotoxicity or regeneration in the injured mouse spinal cord","volume":"29","author":"KA Kigerl","year":"2009","journal-title":"J Neurosci"},{"key":"ppat.1010302.ref101","doi-asserted-by":"crossref","first-page":"1211","DOI":"10.1038\/nn.3469","article-title":"M2 microglia and macrophages drive oligodendrocyte differentiation during CNS remyelination","volume":"16","author":"VE Miron","year":"2013","journal-title":"Nat Neurosci"},{"key":"ppat.1010302.ref102","doi-asserted-by":"crossref","first-page":"217","DOI":"10.1038\/s41467-018-08140-7","article-title":"Chi3l3 induces oligodendrogenesis in an experimental model of autoimmune neuroinflammation.","volume":"10","author":"SC Starossom","year":"2019","journal-title":"Nat Commun"},{"key":"ppat.1010302.ref103","doi-asserted-by":"crossref","first-page":"711","DOI":"10.1016\/S0896-6273(02)00826-7","article-title":"Arginase I and polyamines act downstream from cyclic AMP in overcoming inhibition of axonal growth MAG and myelin in vitro","volume":"35","author":"D Cai","year":"2002","journal-title":"Neuron"},{"key":"ppat.1010302.ref104","doi-asserted-by":"crossref","first-page":"9545","DOI":"10.1523\/JNEUROSCI.1175-09.2009","article-title":"Increased synthesis of spermidine as a result of upregulation of arginase I promotes axonal regeneration in culture and in vivo","volume":"29","author":"K Deng","year":"2009","journal-title":"J Neurosci"},{"key":"ppat.1010302.ref105","doi-asserted-by":"crossref","first-page":"35","DOI":"10.1016\/S0022-1759(98)00170-7","article-title":"A statistically defined endpoint titer determination method for immunoassays","volume":"221","author":"A Frey","year":"1998","journal-title":"J Immunol Methods"},{"key":"ppat.1010302.ref106","doi-asserted-by":"crossref","first-page":"57","DOI":"10.1016\/S0014-4894(02)00036-X","article-title":"Detection of bird schistosomes in lakes by PCR and filter-hybridization","volume":"101","author":"J Hertel","year":"2002","journal-title":"Exp Parasitol"},{"key":"ppat.1010302.ref107","article-title":"Elevated plus maze for mice.","volume":"1088","author":"M Komada","year":"2008","journal-title":"J Vis Exp"},{"key":"ppat.1010302.ref108","doi-asserted-by":"crossref","unstructured":"Riebe CJ, Wotjak CT. A practical guide to evaluating anxiety-related behavior in rodents. In: Szallasi A, B\u00edr\u00f3 T, editors. TRP Channels in Drug Discovery. 2012. pp. 167\u2013185. doi: 10.1007\/978-1-62703-095-3_10","DOI":"10.1007\/978-1-62703-095-3_10"},{"key":"ppat.1010302.ref109","doi-asserted-by":"crossref","unstructured":"Curzon P, Zhang M, Radek RJ, Fox GB. The behavioral assessment of sensorimotor processes in the mouse: Acoustic startle, sensory gating, locomotor activity, rotarod, and beam walking. In: Buccafusco J, editor. Methods of Behavior Analysis in Neuroscience. 2009. pp. 171\u2013176. Available: http:\/\/www.ncbi.nlm.nih.gov\/pubmed\/21204341","DOI":"10.1201\/NOE1420052343.ch8"},{"key":"ppat.1010302.ref110","doi-asserted-by":"crossref","unstructured":"H\u00f6lter SM, Glasl L. High-throughput mouse phenotyping. In: Lane E, Dunnett S, editors. Animal Models of Movement Disorders. 2011. pp. 109\u2013133. doi: 10.1007\/978-1-61779-298-4_7","DOI":"10.1007\/978-1-61779-298-4_7"},{"key":"ppat.1010302.ref111","doi-asserted-by":"crossref","first-page":"583","DOI":"10.1007\/s11357-013-9589-9","article-title":"Battery of behavioral tests in mice that models age-associated changes in human motor function.","volume":"36","author":"JN Justice","year":"2014","journal-title":"Age (Omaha)."},{"key":"ppat.1010302.ref112","doi-asserted-by":"crossref","unstructured":"Mann A, Chesselet MF. Techniques for motor assessment in rodents. In: LeDoux M, editor. Movement Disorders. 2014. pp. 139\u2013157. doi: 10.1016\/C2012-0-00370-5","DOI":"10.1016\/B978-0-12-405195-9.00008-1"},{"key":"ppat.1010302.ref113","doi-asserted-by":"crossref","first-page":"122","DOI":"10.1038\/nprot.2006.20","article-title":"Digging and marble burying in mice: Simple methods for in vivo identification of biological impacts","volume":"1","author":"RM Deacon","year":"2006","journal-title":"Nat Protoc"},{"key":"ppat.1010302.ref114","doi-asserted-by":"crossref","first-page":"105","DOI":"10.1007\/978-1-4939-8994-2_10","article-title":"The Y-maze for assessment of spatial working and reference memory in mice.","author":"A-K Kraeuter","year":"2019","journal-title":"Pre-Clinical Models"},{"key":"ppat.1010302.ref115","article-title":"Measuring the strength of mice.","volume":"2610","author":"RM Deacon","year":"2013","journal-title":"J Vis Exp"},{"key":"ppat.1010302.ref116","article-title":"Low-cost protocol of footprint analysis and hanging box test for mice applied the chronic restraint stress.","volume":"59027","author":"H Sugimoto","year":"2019","journal-title":"J Vis Exp"},{"key":"ppat.1010302.ref117","doi-asserted-by":"crossref","DOI":"10.1002\/0471142301.ns0810as14","article-title":"Rodent models of depression: Forced swimming and tail suspension behavioral despair tests in rats and mice.","volume":"14","author":"RD Porsolt","year":"2001","journal-title":"Curr Protoc Neurosci."},{"key":"ppat.1010302.ref118","article-title":"Isolation of brain and spinal cord mononuclear culls using Percoll gradients","volume":"2348","author":"PA Pino","year":"2011","journal-title":"J Vis Exp"},{"key":"ppat.1010302.ref119","article-title":"FastQC: A quality control tool for high throughput sequence data","author":"S. Andrews","year":"2010"},{"key":"ppat.1010302.ref120","doi-asserted-by":"crossref","first-page":"2114","DOI":"10.1093\/bioinformatics\/btu170","article-title":"Trimmomatic: a flexible trimmer for Illumina sequence data.","volume":"30","author":"AM Bolger","year":"2014","journal-title":"Bioinformatics"},{"key":"ppat.1010302.ref121","doi-asserted-by":"crossref","first-page":"323","DOI":"10.1186\/1471-2105-12-323","article-title":"RSEM: accurate transcript quantification from RNA-Seq data with or without a reference genome","volume":"12","author":"B Li","year":"2011","journal-title":"BMC Bioinformatics"},{"key":"ppat.1010302.ref122","doi-asserted-by":"crossref","first-page":"550","DOI":"10.1186\/s13059-014-0550-8","article-title":"Moderated estimation of fold change and dispersion for RNA-seq data with DESeq2","volume":"15","author":"MI Love","year":"2014","journal-title":"Genome Biol"},{"key":"ppat.1010302.ref123","doi-asserted-by":"crossref","first-page":"1494","DOI":"10.1038\/nprot.2013.084","article-title":"De novo transcript sequence reconstruction from RNA-seq using the Trinity platform for reference generation and analysis.","volume":"8","author":"BJ Haas","year":"2013","journal-title":"Nat Protoc"},{"key":"ppat.1010302.ref124","doi-asserted-by":"crossref","first-page":"D457","DOI":"10.1093\/nar\/gkv1070","article-title":"KEGG as a reference resource for gene and protein annotation","volume":"44","author":"M Kanehisa","year":"2016","journal-title":"Nucleic Acids Res"},{"key":"ppat.1010302.ref125","doi-asserted-by":"crossref","first-page":"27","DOI":"10.1093\/nar\/28.1.27","article-title":"KEGG: kyoto encyclopedia of genes and genomes","volume":"28","author":"M Kanehisa","year":"2000","journal-title":"Nucleic Acids Res"},{"key":"ppat.1010302.ref126","article-title":"Differential proteomic analysis of laser-microdissected penetration glands of avian schistosome cercariae with a focus on proteins involved in host invasion","author":"O Vondr\u00e1\u010dek","year":"2022","journal-title":"Int J Parasitol"},{"key":"ppat.1010302.ref127","doi-asserted-by":"crossref","first-page":"319","DOI":"10.1038\/nmeth.2834","article-title":"Minimal, encapsulated proteomic-sample processing applied to copy-number estimation in eukaryotic cells.","volume":"11","author":"NA Kulak","year":"2014","journal-title":"Nat Methods"},{"key":"ppat.1010302.ref128","doi-asserted-by":"crossref","first-page":"2513","DOI":"10.1074\/mcp.M113.031591","article-title":"Accurate proteome-wide label-free quantification by delayed normalization and maximal peptide ratio extraction, termed MaxLFQ","volume":"13","author":"J Cox","year":"2014","journal-title":"Mol Cell Proteomics"},{"key":"ppat.1010302.ref129","doi-asserted-by":"crossref","first-page":"731","DOI":"10.1038\/nmeth.3901","article-title":"The Perseus computational platform for comprehensive analysis of (prote)omics data.","volume":"13","author":"S Tyanova","year":"2016","journal-title":"Nat Methods"},{"key":"ppat.1010302.ref130","doi-asserted-by":"crossref","first-page":"1709","DOI":"10.1038\/nprot.2015.085","article-title":"Advanced CUBIC protocols for whole-brain and whole-body clearing and imaging.","volume":"10","author":"EA Susaki","year":"2015","journal-title":"Nat Protoc"},{"key":"ppat.1010302.ref131","doi-asserted-by":"crossref","first-page":"e83","DOI":"10.1002\/cpim.83","article-title":"Using Evans blue dye to determine blood-brain barrier integrity in rodents.","volume":"126","author":"S Goldim MP de","year":"2019","journal-title":"Curr Protoc Immunol"}],"updated-by":[{"DOI":"10.1371\/journal.ppat.1010302","type":"new_version","label":"New version","source":"publisher","updated":{"date-parts":[[2022,2,16]],"date-time":"2022-02-16T00:00:00Z","timestamp":1644969600000}}],"container-title":["PLOS Pathogens"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/dx.plos.org\/10.1371\/journal.ppat.1010302","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,11,16]],"date-time":"2023-11-16T23:56:25Z","timestamp":1700178985000},"score":1,"resource":{"primary":{"URL":"https:\/\/dx.plos.org\/10.1371\/journal.ppat.1010302"}},"subtitle":[],"editor":[{"given":"Elise","family":"O\u2019Connell","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2022,2,4]]},"references-count":131,"journal-issue":{"issue":"2","published-online":{"date-parts":[[2022,2,4]]}},"URL":"https:\/\/doi.org\/10.1371\/journal.ppat.1010302","relation":{"new_version":[{"id-type":"doi","id":"10.1371\/journal.ppat.1010302","asserted-by":"object"}]},"ISSN":["1553-7374"],"issn-type":[{"value":"1553-7374","type":"electronic"}],"subject":[],"published":{"date-parts":[[2022,2,4]]}}}