{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,7]],"date-time":"2026-10-07T15:27:49Z","timestamp":1791386869705,"version":"4.3.3"},"update-to":[{"DOI":"10.1371\/journal.ppat.1010329","type":"new_version","label":"New version","source":"publisher","updated":{"date-parts":[[2022,9,20]],"date-time":"2022-09-20T00:00:00Z","timestamp":1663632000000}}],"reference-count":96,"publisher":"Public Library of Science (PLoS)","issue":"9","license":[{"start":{"date-parts":[[2022,9,8]],"date-time":"2022-09-08T00:00:00Z","timestamp":1662595200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100003246","name":"Nederlandse Organisatie voor Wetenschappelijk Onderzoek","doi-asserted-by":"publisher","award":["016.VICI.170.090"],"award-info":[{"award-number":["016.VICI.170.090"]}],"id":[{"id":"10.13039\/501100003246","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001826","name":"ZonMw","doi-asserted-by":"publisher","award":["04510011910045"],"award-info":[{"award-number":["04510011910045"]}],"id":[{"id":"10.13039\/501100001826","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["www.plospathogens.org"],"crossmark-restriction":false},"short-container-title":["PLoS Pathog"],"abstract":"<jats:p>\n                    Efficient virus replication in\n                    <jats:italic>Aedes<\/jats:italic>\n                    vector mosquitoes is essential for the transmission of arboviral diseases such as dengue virus (DENV) in human populations. Like in vertebrates, virus-host protein-protein interactions are essential for viral replication and immune evasion in the mosquito vector. Here, 79 mosquito host proteins interacting with DENV non-structural proteins NS1 and NS5 were identified by label-free mass spectrometry, followed by a functional screening. We confirmed interactions with host factors previously observed in mammals, such as the oligosaccharyltransferase complex, and we identified protein-protein interactions that seem to be specific for mosquitoes. Among the interactors, the double-stranded RNA (dsRNA) binding protein Loquacious (Loqs), an RNA interference (RNAi) cofactor, was found to be essential for efficient replication of DENV and Zika virus (ZIKV) in mosquito cells. Loqs did not affect viral RNA stability or translation of a DENV replicon and its proviral activity was independent of its RNAi regulatory activity. Interestingly, Loqs colocalized with DENV dsRNA replication intermediates in infected cells and directly interacted with high affinity with DENV RNA in the 3\u2019 untranslated region\n                    <jats:italic>in vitro<\/jats:italic>\n                    (K\n                    <jats:sub>D<\/jats:sub>\n                    = 48\u201362 nM). Our study provides an interactome for DENV NS1 and NS5 and identifies Loqs as a key proviral host factor in mosquitoes. We propose that DENV hijacks a factor of the RNAi mechanism for replication of its own RNA.\n                  <\/jats:p>","DOI":"10.1371\/journal.ppat.1010329","type":"journal-article","created":{"date-parts":[[2022,9,8]],"date-time":"2022-09-08T13:32:49Z","timestamp":1662643969000},"page":"e1010329","update-policy":"https:\/\/doi.org\/10.1371\/journal.ppat.corrections_policy","source":"Crossref","is-referenced-by-count":22,"title":["Arbovirus-vector protein interactomics identifies Loquacious as a co-factor for dengue virus replication in Aedes mosquitoes"],"prefix":"10.1371","volume":"18","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-6889-0818","authenticated-orcid":true,"given":"Benoit","family":"Besson","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Oscar M.","family":"Lezcano","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gijs J.","family":"Overheul","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kirsten","family":"Janssen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cornelia G.","family":"Spruijt","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michiel","family":"Vermeulen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jieqiong","family":"Qu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0221-4689","authenticated-orcid":true,"given":"Ronald P.","family":"van Rij","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"340","published-online":{"date-parts":[[2022,9,8]]},"reference":[{"key":"ppat.1010329.ref001","doi-asserted-by":"crossref","first-page":"504","DOI":"10.1038\/nature12060","article-title":"The global distribution and burden of dengue","volume":"496","author":"S Bhatt","year":"2013","journal-title":"Nature"},{"key":"ppat.1010329.ref002","doi-asserted-by":"crossref","first-page":"20696","DOI":"10.2807\/1560-7917.ES2014.19.6.20696","article-title":"Climatic suitability of Aedes albopictus in Europe referring to climate change projections: comparison of mechanistic and correlative niche modelling approaches","volume":"19","author":"D Fischer","year":"2014","journal-title":"Eurosurveillance"},{"key":"ppat.1010329.ref003","doi-asserted-by":"crossref","first-page":"e08347","DOI":"10.2807\/1560-7917.ES.2018.23.47.1700837","article-title":"Arbovirus surveillance: first dengue virus 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Wang","year":"2021","journal-title":"Biomed Pharmacother"},{"key":"ppat.1010329.ref007","doi-asserted-by":"crossref","first-page":"125","DOI":"10.1038\/nrmicro.2017.170","article-title":"Rewiring cellular networks by members of the Flaviviridae family","volume":"16","author":"CJ Neufeldt","year":"2018","journal-title":"Nat Rev Microbiol"},{"key":"ppat.1010329.ref008","doi-asserted-by":"crossref","first-page":"17","DOI":"10.1007\/s00284-020-02284-w","article-title":"Current Understanding of the Pathogenesis of Dengue Virus Infection","volume":"78","author":"P Bhatt","year":"2021","journal-title":"Curr Microbiol"},{"key":"ppat.1010329.ref009","doi-asserted-by":"crossref","first-page":"227","DOI":"10.1146\/annurev-virology-101416-041848","article-title":"The Good, the Bad, and the Shocking: The Multiple Roles of Dengue Virus Nonstructural Protein 1 in Protection and Pathogenesis","volume":"5","author":"DR Glasner","year":"2018","journal-title":"Annu Rev 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the membranous replication organelle","volume":"15","author":"A P\u0142aszczyca","year":"2019","journal-title":"PLOS Pathog"},{"key":"ppat.1010329.ref056","doi-asserted-by":"crossref","first-page":"jcs231423","DOI":"10.1242\/jcs.231423","article-title":"Bring it back, bring it back, don\u2019t take it away from me\u2013the sorting receptor RER1","volume":"133","author":"W Annaert","year":"2020","journal-title":"J Cell Sci"},{"key":"ppat.1010329.ref057","doi-asserted-by":"crossref","first-page":"95","DOI":"10.3389\/fmolb.2020.00095","article-title":"Hsp60 Post-translational Modifications: Functional and Pathological Consequences","volume":"7","author":"C Caruso Bavisotto","year":"2020","journal-title":"Front Mol Biosci"},{"key":"ppat.1010329.ref058","doi-asserted-by":"crossref","first-page":"12758","DOI":"10.1038\/srep12758","article-title":"The heat shock response restricts virus infection in Drosophila","volume":"5","author":"SH Merkling","year":"2015","journal-title":"Sci 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Lin","year":"2017","journal-title":"MBio"},{"key":"ppat.1010329.ref062","doi-asserted-by":"crossref","first-page":"e1008617","DOI":"10.1371\/journal.pgen.1008617","article-title":"Inhibition of the oligosaccharyl transferase in Caenorhabditis elegans that compromises ER proteostasis suppresses p38-dependent protection against pathogenic bacteria","volume":"16","author":"D-E Jeong","year":"2020","journal-title":"PLOS Genet"},{"key":"ppat.1010329.ref063","doi-asserted-by":"crossref","first-page":"24","DOI":"10.1104\/pp.113.215509","article-title":"Specialized Roles of the Conserved Subunit OST3\/6 of the Oligosaccharyltransferase Complex in Innate Immunity and Tolerance to Abiotic Stresses","volume":"162","author":"A Farid","year":"2013","journal-title":"Plant Physiol"},{"key":"ppat.1010329.ref064","doi-asserted-by":"crossref","first-page":"952","DOI":"10.3390\/v13060952","article-title":"RNA-Binding Proteins at the Host-Pathogen Interface Targeting Viral Regulatory 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