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However, the biologically most relevant catarrhal disease stage and <jats:italic>B<\/jats:italic>. <jats:italic>pertussis<\/jats:italic> transmission has not been adequately reproduced in adult mice due to limited proliferation of the human-adapted pathogen on murine nasopharyngeal mucosa. We used immunodeficient C57BL\/6J MyD88 KO mice to achieve <jats:italic>B<\/jats:italic>. <jats:italic>pertussis<\/jats:italic> proliferation to human-like high counts of 10<jats:sup>8<\/jats:sup> viable bacteria per nasal cavity to elicit rhinosinusitis accompanied by robust shedding and transmission of <jats:italic>B<\/jats:italic>. <jats:italic>pertussis<\/jats:italic> bacteria to adult co-housed MyD88 KO mice. Experiments with a comprehensive set of <jats:italic>B<\/jats:italic>. <jats:italic>pertussis<\/jats:italic> mutants revealed that pertussis toxin, adenylate cyclase toxin-hemolysin, the T3SS effector BteA\/BopC and several other known virulence factors were dispensable for nasal cavity infection and <jats:italic>B<\/jats:italic>. <jats:italic>pertussis<\/jats:italic> transmission in the immunocompromised MyD88 KO mice. In contrast, mutants lacking the filamentous hemagglutinin (FhaB) or fimbriae (Fim) adhesins infected the nasal cavity poorly, shed at low levels and failed to productively infect co-housed MyD88 KO or C57BL\/6J mice. FhaB and fimbriae thus appear to play a critical role in <jats:italic>B<\/jats:italic>. <jats:italic>pertussis<\/jats:italic> transmission. The here-described novel murine model of <jats:italic>B<\/jats:italic>. <jats:italic>pertussis<\/jats:italic>-induced nasal catarrh opens the way to genetic dissection of host mechanisms involved in <jats:italic>B<\/jats:italic>. <jats:italic>pertussis<\/jats:italic> shedding and to validation of key bacterial transmission factors that ought to be targeted by future pertussis vaccines.<\/jats:p>","DOI":"10.1371\/journal.ppat.1010402","type":"journal-article","created":{"date-parts":[[2022,4,8]],"date-time":"2022-04-08T17:36:00Z","timestamp":1649439360000},"page":"e1010402","update-policy":"https:\/\/doi.org\/10.1371\/journal.ppat.corrections_policy","source":"Crossref","is-referenced-by-count":29,"title":["The Fim and FhaB adhesins play a crucial role in nasal cavity infection and Bordetella pertussis transmission in a novel mouse catarrhal infection model"],"prefix":"10.1371","volume":"18","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-9755-7715","authenticated-orcid":true,"given":"Jana","family":"Holubova","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ondrej","family":"Stanek","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Attila","family":"Juhasz","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Illiassou","family":"Hamidou 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