{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T13:35:12Z","timestamp":1790775312616,"version":"4.1.0"},"update-to":[{"DOI":"10.1371\/journal.ppat.1010427","type":"new_version","label":"New version","source":"publisher","updated":{"date-parts":[[2022,10,4]],"date-time":"2022-10-04T00:00:00Z","timestamp":1664841600000}}],"reference-count":63,"publisher":"Public Library of Science (PLoS)","issue":"9","license":[{"start":{"date-parts":[[2022,9,19]],"date-time":"2022-09-19T00:00:00Z","timestamp":1663545600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001349","name":"National Medical Research Council","doi-asserted-by":"crossref","award":["ZRRF\/007\/2017"],"award-info":[{"award-number":["ZRRF\/007\/2017"]}],"id":[{"id":"10.13039\/501100001349","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100001665","name":"Agence Nationale de la Recherche","doi-asserted-by":"crossref","award":["ANR-20-CE15-0006"],"award-info":[{"award-number":["ANR-20-CE15-0006"]}],"id":[{"id":"10.13039\/501100001665","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100003246","name":"Dutch Research Council","doi-asserted-by":"crossref","award":["Rubicon fellowship"],"award-info":[{"award-number":["Rubicon fellowship"]}],"id":[{"id":"10.13039\/501100003246","id-type":"DOI","asserted-by":"crossref"}]},{"name":"French Ministry of higher education and research","award":["PhD fellowship"],"award-info":[{"award-number":["PhD fellowship"]}]},{"name":"Agency for Science Technology and Research","award":["via Emerging Infectious Diseases (EID) Signature Research Programme at Duke-NUS"],"award-info":[{"award-number":["via Emerging Infectious Diseases (EID) Signature Research Programme at Duke-NUS"]}]}],"content-domain":{"domain":["www.plospathogens.org"],"crossmark-restriction":false},"short-container-title":["PLoS Pathog"],"abstract":"<jats:p>\n                    Dengue viruses (DENV) are expanding global pathogens that are transmitted through the bite of mosquitoes, mostly\n                    <jats:italic>Aedes aegypti<\/jats:italic>\n                    . As RNA viruses, DENV rely on RNA-binding proteins (RBPs) to complete their life cycle. Alternatively, RBPs can act as restriction factors that prevent DENV multiplication. While the importance of RBPs is well-supported in humans, there is a dearth of information about their influence on DENV transmission by mosquitoes. Such knowledge could be harnessed to design novel, effective interventions against DENV. Here, we successfully adapted RNA-affinity chromatography coupled with mass spectrometry\u2013a technique initially developed in mammalian cells\u2013to identify RBPs in\n                    <jats:italic>Ae<\/jats:italic>\n                    .\n                    <jats:italic>aegypti<\/jats:italic>\n                    cells. We identified fourteen RBPs interacting with DENV serotype 2 3\u2019UTR, which is involved in the viral multiplication and produces subgenomic flaviviral RNA (sfRNA). We validated the RNA affinity results for two RBPs by confirming that AePur binds the 3\u2019UTR, whereas AeStaufen interacts with both 3\u2019UTR and sfRNA. Using\n                    <jats:italic>in vivo<\/jats:italic>\n                    functional evaluation, we determined that RBPs like AeRan, AeExoRNase, and AeRNase have pro-viral functions, whereas AeGTPase, AeAtu, and AePur have anti-viral functions in mosquitoes. Furthermore, we showed that human and mosquito Pur homologs have a shared affinity to DENV2 RNA, although the anti-viral effect is specific to the mosquito protein. Importantly, we revealed that AeStaufen mediates a reduction of gRNA and sfRNA copies in several mosquito tissues, including the salivary glands and that AeStaufen-mediated sfRNA reduction diminishes the concentration of transmission-enhancing sfRNA in saliva, thereby revealing AeStaufen\u2019s role in DENV transmission. By characterizing the first RBPs that associate with DENV2 3\u2019UTR in mosquitoes, our study unravels new pro- and anti-viral targets for the design of novel therapeutic interventions as well as provides foundation for studying the role of RBPs in virus-vector interactions.\n                  <\/jats:p>","DOI":"10.1371\/journal.ppat.1010427","type":"journal-article","created":{"date-parts":[[2022,9,19]],"date-time":"2022-09-19T13:43:16Z","timestamp":1663594996000},"page":"e1010427","update-policy":"https:\/\/doi.org\/10.1371\/journal.ppat.corrections_policy","source":"Crossref","is-referenced-by-count":16,"title":["Characterization of dengue virus 3\u2019UTR RNA binding proteins in mosquitoes reveals that AeStaufen reduces subgenomic flaviviral RNA in saliva"],"prefix":"10.1371","volume":"18","author":[{"given":"Shih-Chia","family":"Yeh","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mayra","family":"Diosa-Toro","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wei-Lian","family":"Tan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Florian","family":"Rachenne","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Arthur","family":"Hain","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Celestia Pei Xuan","family":"Yeo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"In\u00e8s","family":"Bribes","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Benjamin Wong Wei","family":"Xiang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gayathiri","family":"Sathiamoorthy Kannan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Menchie Casayuran","family":"Manuel","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Doroth\u00e9e","family":"Miss\u00e9","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yu Keung","family":"Mok","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1110-9039","authenticated-orcid":true,"given":"Julien","family":"Pompon","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"340","published-online":{"date-parts":[[2022,9,19]]},"reference":[{"key":"ppat.1010427.ref001","doi-asserted-by":"crossref","first-page":"129","DOI":"10.1038\/nm0204-129","article-title":"Cities spawn epidemic dengue viruses","volume":"10","author":"DJ Gubler","year":"2004","journal-title":"Natural Medicines"},{"key":"ppat.1010427.ref002","doi-asserted-by":"crossref","first-page":"796","DOI":"10.1038\/s41564-020-0714-0","article-title":"The continued threat of emerging flaviviruses","volume":"5","author":"TC Pierson","year":"2020","journal-title":"Nature 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