{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,2]],"date-time":"2026-06-02T20:05:32Z","timestamp":1780430732061,"version":"3.54.1"},"update-to":[{"DOI":"10.1371\/journal.ppat.1010502","type":"new_version","label":"New version","source":"publisher","updated":{"date-parts":[[2022,11,11]],"date-time":"2022-11-11T00:00:00Z","timestamp":1668124800000}}],"reference-count":39,"publisher":"Public Library of Science (PLoS)","issue":"11","license":[{"start":{"date-parts":[[2022,11,1]],"date-time":"2022-11-01T00:00:00Z","timestamp":1667260800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/publicdomain\/zero\/1.0\/"}],"funder":[{"name":"Division of Intramural Research, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland"}],"content-domain":{"domain":["www.plospathogens.org"],"crossmark-restriction":false},"short-container-title":["PLoS Pathog"],"abstract":"<jats:p>The atypical I\u03baB family member Bcl3 associates with p50\/NF-\u03baB1 or p52\/NF-\u03baB2 homodimers in the nucleus, and positively or negatively modulates transcription in a context-dependent manner. In mice lacking Bcl3 globally or specifically in CD11c<jats:sup>+<\/jats:sup>cells, we previously reported that<jats:italic>Toxoplasma gondii<\/jats:italic>infection is uniformly fatal and is associated with an impaired Th1 immune response. Since Bcl3 expression in dendritic cells (DC) is pivotal for antigen presentation and since classical DCs (cDC) are major antigen presenting cells, we investigated the role of Bcl3 specifically in cDCs<jats:italic>in vivo<\/jats:italic>by crossing Zbtb46 cre mice with<jats:italic>Bcl3<\/jats:italic><jats:sup><jats:italic>flx\/flx<\/jats:italic><\/jats:sup>mice.<jats:italic>Bcl3<\/jats:italic><jats:sup><jats:italic>flx\/flx<\/jats:italic><\/jats:sup><jats:italic>Zbtb46 cre<\/jats:italic>mice were as susceptible to lethal<jats:italic>T<\/jats:italic>.<jats:italic>gondii<\/jats:italic>infection as total<jats:italic>Bcl3<\/jats:italic><jats:sup><jats:italic>-\/-<\/jats:italic><\/jats:sup>mice and generated poor Th1 immune responses. Consistent with this, compared to wildtype controls, splenic Xcr1<jats:sup>+<\/jats:sup>Bcl3-deficient cDC1 cells were defective in presenting Ova antigen to OT-I cells both for Ova<jats:sub>257-264<\/jats:sub>peptide and after infection with Ovalbumin-expressing<jats:italic>T<\/jats:italic>.<jats:italic>gondii<\/jats:italic>. Moreover, splenic CD4<jats:sup>+<\/jats:sup>and CD8<jats:sup>+<\/jats:sup>T cells from infected<jats:italic>Bcl3<\/jats:italic><jats:sup><jats:italic>flx\/flx<\/jats:italic><\/jats:sup><jats:italic>Zbtb46 cre<\/jats:italic>mice exhibited decreased<jats:italic>T<\/jats:italic>.<jats:italic>gondii<\/jats:italic>-specific priming as revealed by both reduced cytokine production and reduced<jats:italic>T<\/jats:italic>.<jats:italic>gondii<\/jats:italic>-specific tetramer staining.<jats:italic>In vitro<\/jats:italic>differentiation of cDCs from bone marrow progenitors also revealed Bcl3-dependent cDC-specific antigen-presentation activity. Consistent with this, splenocyte single cell RNA seq (scRNAseq) in infected mice revealed Bcl3-dependent expression of genes involved in antigen processing in cDCs. We also identified by scRNAseq, a unique Bcl3-dependent hybrid subpopulation of Zbtb46<jats:sup>+<\/jats:sup>DCs co-expressing the monocyte\/macrophage transcription factor Lysozyme M. This subpopulation exhibited Bcl3-dependent expansion after infection. Likewise, by flow cytometry we identified two<jats:italic>T<\/jats:italic>.<jats:italic>gondii<\/jats:italic>-induced hybrid subpopulations of Bcl3-dependent cDC1 and cDC2 cells both expressing monocyte\/macrophage markers, designated as icDC1 and icDC2. Together, our results indicate that Bcl3 in classical DCs is a major determinant of protective T cell responses and survival in<jats:italic>T<\/jats:italic>.<jats:italic>gondii<\/jats:italic>-infection.<\/jats:p>","DOI":"10.1371\/journal.ppat.1010502","type":"journal-article","created":{"date-parts":[[2022,11,1]],"date-time":"2022-11-01T17:30:48Z","timestamp":1667323848000},"page":"e1010502","update-policy":"https:\/\/doi.org\/10.1371\/journal.ppat.corrections_policy","source":"Crossref","is-referenced-by-count":12,"title":["NF kappa B regulator Bcl3 controls development and function of classical dendritic cells required for resistance to Toxoplasma 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