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To initiate sexual reproduction and to infect the midgut of the mosquito,\n                    <jats:italic>Plasmodium<\/jats:italic>\n                    gametocytes are able to recognize the intestinal environment after being ingested during blood feeding. A shift in temperature, pH change and the presence of the insect-specific compound xanthurenic acid have been shown to be important stimuli perceived by gametocytes to become activated and proceed to sexual reproduction. Here we report that the salivary protein Saglin, previously proposed to be a receptor for the recognition of salivary glands by sporozoites, facilitates\n                    <jats:italic>Plasmodium<\/jats:italic>\n                    colonization of the mosquito midgut, but does not contribute to salivary gland invasion. In mosquito mutants lacking Saglin,\n                    <jats:italic>Plasmodium<\/jats:italic>\n                    infection of\n                    <jats:italic>Anopheles<\/jats:italic>\n                    females is reduced, resulting in impaired transmission of sporozoites at low infection densities. Interestingly, Saglin can be detected in high amounts in the midgut of mosquitoes after blood ingestion, possibly indicating a previously unknown host-pathogen interaction between Saglin and midgut stages of\n                    <jats:italic>Plasmodium<\/jats:italic>\n                    . Furthermore, we were able to show that\n                    <jats:italic>saglin<\/jats:italic>\n                    deletion has no fitness cost in laboratory conditions, suggesting this gene would be an interesting target for gene drive approaches.\n                  <\/jats:p>","DOI":"10.1371\/journal.ppat.1010538","type":"journal-article","created":{"date-parts":[[2023,3,2]],"date-time":"2023-03-02T13:50:04Z","timestamp":1677765004000},"page":"e1010538","update-policy":"https:\/\/doi.org\/10.1371\/journal.ppat.corrections_policy","source":"Crossref","is-referenced-by-count":24,"title":["The salivary protein Saglin facilitates efficient midgut colonization of Anopheles mosquitoes by malaria parasites"],"prefix":"10.1371","volume":"19","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-9108-454X","authenticated-orcid":true,"given":"Dennis","family":"Klug","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Amandine","family":"Gautier","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eric","family":"Calvo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eric","family":"Marois","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"St\u00e9phanie A.","family":"Blandin","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"340","published-online":{"date-parts":[[2023,3,2]]},"reference":[{"key":"ppat.1010538.ref001","volume-title":"World malaria report 2021","author":"WHO","year":"2021"},{"key":"ppat.1010538.ref002","article-title":"Efficiency of salivary gland invasion by malaria sporozoites is controlled by rapid sporozoite destruction in the mosquito haemocoel","author":"JF Hillyer","year":"2007","journal-title":"Int J Parasitol"},{"issue":"5","key":"ppat.1010538.ref003","doi-asserted-by":"crossref","first-page":"574","DOI":"10.4269\/ajtmh.1991.45.574","article-title":"The Number of Sporozoites Produced by Individual Malaria Oocysts","volume":"45","author":"R Rosenberg","year":"1991","journal-title":"Am J Trop Med Hyg"},{"key":"ppat.1010538.ref004","doi-asserted-by":"crossref","first-page":"357","DOI":"10.1016\/j.pt.2015.04.010","article-title":"Active migration and passive transport of malaria parasites.","volume":"31","author":"RG Douglas","year":"2015","journal-title":"Trends in Parasitology"},{"issue":"12","key":"ppat.1010538.ref005","doi-asserted-by":"crossref","first-page":"e195","DOI":"10.1371\/journal.ppat.0030195","article-title":"Progression of Plasmodium berghei through Anopheles stephensi is density-dependent.","volume":"3","author":"RE Sinden","year":"2007","journal-title":"PLoS Pathog."},{"issue":"11","key":"ppat.1010538.ref006","doi-asserted-by":"crossref","first-page":"1229","DOI":"10.1016\/j.ijpara.2010.05.005","article-title":"Invasion of mosquito salivary glands by malaria parasites: prerequisites and defense strategies","volume":"40","author":"A-K Mueller","year":"2010","journal-title":"Int J Parasitol"},{"issue":"4","key":"ppat.1010538.ref007","doi-asserted-by":"crossref","first-page":"687","DOI":"10.4269\/ajtmh.1985.34.687","article-title":"Inability of Plasmodium Knowlesi Sporozoites to Invade Anopheles Freeborni Salivary Glands","volume":"34","author":"R. 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