{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,13]],"date-time":"2026-10-13T19:06:44Z","timestamp":1791918404667,"version":"4.3.4"},"reference-count":41,"publisher":"Public Library of Science (PLoS)","issue":"6","license":[{"start":{"date-parts":[[2022,6,9]],"date-time":"2022-06-09T00:00:00Z","timestamp":1654732800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"National Key Research and Development Plan of China","award":["2021YFC2300200-4, 2020YFC1200104, 2018YFA0507202, 2019YFC1200201"],"award-info":[{"award-number":["2021YFC2300200-4, 2020YFC1200104, 2018YFA0507202, 2019YFC1200201"]}]},{"DOI":"10.13039\/501100001809","name":"Natural Science Foundation of China","doi-asserted-by":"crossref","award":["32188101, 31825001, 81730063, 82102389, 81961160737"],"award-info":[{"award-number":["32188101, 31825001, 81730063, 82102389, 81961160737"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"crossref"}]},{"name":"Tsinghua University Spring Breeze Fund","award":["2020Z99CFG017"],"award-info":[{"award-number":["2020Z99CFG017"]}]},{"name":"Open Foundation of NHC Key Laboratory of Tropical Disease Control, Hainan Medical University","award":["2021NHCTDCKFKT22013"],"award-info":[{"award-number":["2021NHCTDCKFKT22013"]}]},{"name":"Shenzhen San-Ming Project for prevention and research on vector-borne diseases","award":["SZSM201611064"],"award-info":[{"award-number":["SZSM201611064"]}]},{"name":"Yunnan Cheng- gong expert workstation","award":["202005AF150034"],"award-info":[{"award-number":["202005AF150034"]}]},{"name":"G.C. Innovation Team Project of Yunnan Science and Technology Department","award":["202105AE160020"],"award-info":[{"award-number":["202105AE160020"]}]},{"name":"Young Elite Scientists Sponsorship Program","award":["2021QNRC001"],"award-info":[{"award-number":["2021QNRC001"]}]}],"content-domain":{"domain":["www.plospathogens.org"],"crossmark-restriction":false},"short-container-title":["PLoS Pathog"],"abstract":"<jats:p>Arboviruses are etiological agents of various severe human diseases that place a tremendous burden on global public health and the economy; compounding this issue is the fact that effective prophylactics and therapeutics are lacking for most arboviruses. Herein, we identified 2 bacterial lipases secreted by a <jats:italic>Chromobacterium<\/jats:italic> bacterium isolated from <jats:italic>Aedes aegypti<\/jats:italic> midgut, <jats:italic>Chromobacterium<\/jats:italic> antiviral effector-1 (<jats:italic>Cb<\/jats:italic>AE-1) and <jats:italic>Cb<\/jats:italic>AE-2, with broad-spectrum virucidal activity against mosquito-borne viruses, such as dengue virus (DENV), Zika virus (ZIKV), Japanese encephalitis virus (JEV), yellow fever virus (YFV) and Sindbis virus (SINV). The <jats:italic>Cb<\/jats:italic>AEs potently blocked viral infection in the extracellular milieu through their lipase activity. Mechanistic studies showed that this lipase activity directly disrupted the viral envelope structure, thus inactivating infectivity. A mutation in the lipase motif of <jats:italic>Cb<\/jats:italic>AE-1 fully abrogated the virucidal ability. Furthermore, <jats:italic>Cb<\/jats:italic>AEs also exert lipase-dependent entomopathogenic activity in mosquitoes. The anti-arboviral and entomopathogenic properties of <jats:italic>Cb<\/jats:italic>AEs render them potential candidates for the development of novel transmission control strategies against vector-borne diseases.<\/jats:p>","DOI":"10.1371\/journal.ppat.1010552","type":"journal-article","created":{"date-parts":[[2022,6,9]],"date-time":"2022-06-09T17:28:59Z","timestamp":1654795739000},"page":"e1010552","update-policy":"https:\/\/doi.org\/10.1371\/journal.ppat.corrections_policy","source":"Crossref","is-referenced-by-count":26,"title":["Lipases secreted by a gut bacterium inhibit arbovirus transmission in mosquitoes"],"prefix":"10.1371","volume":"18","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-7724-8317","authenticated-orcid":true,"given":"Xi","family":"Yu","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Liangqin","family":"Tong","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Liming","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yun","family":"Yang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaoping","family":"Xiao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yibin","family":"Zhu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Penghua","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7447-5488","authenticated-orcid":true,"given":"Gong","family":"Cheng","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"340","published-online":{"date-parts":[[2022,6,9]]},"reference":[{"issue":"10","key":"ppat.1010552.ref001","doi-asserted-by":"crossref","first-page":"789","DOI":"10.1038\/nrmicro1006","article-title":"Transmission cycles, host range, evolution and emergence of arboviral disease","volume":"2","author":"SC Weaver","year":"2004","journal-title":"Nat Rev Microbiol"},{"key":"ppat.1010552.ref002","doi-asserted-by":"crossref","first-page":"22","DOI":"10.3389\/fmicb.2019.00022","article-title":"Arbovirus-Mosquito Vector-Host Interactions and the Impact on Transmission and Disease Pathogenesis of Arboviruses","volume":"10","author":"YS Huang","year":"2019","journal-title":"Front Microbiol."},{"key":"ppat.1010552.ref003","doi-asserted-by":"crossref","first-page":"25","DOI":"10.1016\/j.ijid.2017.11.026","article-title":"Global risk mapping for major diseases transmitted by Aedes aegypti and Aedes albopictus","volume":"67","author":"S Leta","year":"2018","journal-title":"Int J Infect Dis"},{"issue":"2","key":"ppat.1010552.ref004","doi-asserted-by":"crossref","first-page":"328","DOI":"10.1016\/j.antiviral.2009.10.008","article-title":"Present and future arboviral threats","volume":"85","author":"SC Weaver","year":"2010","journal-title":"Antiviral Res"},{"issue":"7446","key":"ppat.1010552.ref005","doi-asserted-by":"crossref","first-page":"504","DOI":"10.1038\/nature12060","article-title":"The global distribution and burden of dengue","volume":"496","author":"S Bhatt","year":"2013","journal-title":"Nature"},{"issue":"16","key":"ppat.1010552.ref006","doi-asserted-by":"crossref","first-page":"1513","DOI":"10.1056\/NEJMoa1605564","article-title":"Guillain-Barre Syndrome Associated with Zika Virus Infection in Colombia","volume":"375","author":"B Parra","year":"2016","journal-title":"N Engl J Med"},{"issue":"9611","key":"ppat.1010552.ref007","doi-asserted-by":"crossref","first-page":"500","DOI":"10.1016\/S0140-6736(08)60238-X","article-title":"Pathogenic flaviviruses","volume":"371","author":"EA Gould","year":"2008","journal-title":"Lancet"},{"issue":"6","key":"ppat.1010552.ref008","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1080\/21645515.2017.1285472","article-title":"Japanese encephalitis vaccines: Immunogenicity, protective efficacy, effectiveness, and impact on the burden of disease","volume":"13","author":"NR Hegde","year":"2017","journal-title":"Hum Vaccin Immunother"},{"key":"ppat.1010552.ref009","doi-asserted-by":"crossref","first-page":"27","DOI":"10.1111\/j.1600-065X.2008.00670.x","article-title":"Host responses to alphavirus infection","volume":"225","author":"KD Ryman","year":"2008","journal-title":"Immunol Rev"},{"issue":"8","key":"ppat.1010552.ref010","doi-asserted-by":"crossref","first-page":"1519","DOI":"10.1016\/j.clinthera.2017.07.001","article-title":"Progress and Works in Progress: Update on Flavivirus Vaccine Development","volume":"39","author":"MH Collins","year":"2017","journal-title":"Clin Ther"},{"issue":"4","key":"ppat.1010552.ref011","doi-asserted-by":"crossref","first-page":"307","DOI":"10.1016\/j.chom.2011.09.006","article-title":"Native microbiota shape insect vector competence for human pathogens","volume":"10","author":"CM Cirimotich","year":"2011","journal-title":"Cell Host Microbe"},{"key":"ppat.1010552.ref012","article-title":"A non-live preparation of Chromobacterium sp. Panama (Csp_P) is a highly effective larval mosquito biopesticide","author":"EP Caragata","year":"2020","journal-title":"Appl Environ Microbiol"},{"issue":"10","key":"ppat.1010552.ref013","doi-asserted-by":"crossref","DOI":"10.1371\/journal.ppat.1004398","article-title":"Chromobacterium Csp_P Reduces Malaria and Dengue Infection in Vector Mosquitoes and Has Entomopathogenic and In Vitro Anti-pathogen Activities","volume":"10","author":"JL Ramirez","year":"2014","journal-title":"Plos Pathog."},{"key":"ppat.1010552.ref014","first-page":"8","article-title":"Chromobacterium spp. mediate their anti-Plasmodium activity through secretion of the histone deacetylase inhibitor romidepsin","author":"RG Saraiva","year":"2018","journal-title":"Sci Rep-Uk"},{"issue":"1","key":"ppat.1010552.ref015","doi-asserted-by":"crossref","first-page":"101","DOI":"10.1016\/j.chom.2018.11.004","article-title":"A Gut Commensal Bacterium Promotes Mosquito Permissiveness to Arboviruses","volume":"25","author":"P Wu","year":"2019","journal-title":"Cell Host Microbe"},{"key":"ppat.1010552.ref016","doi-asserted-by":"crossref","first-page":"3","DOI":"10.1007\/978-1-4939-8672-9_1","article-title":"Lipases: An Overview","volume":"1835","author":"L Casas-Godoy","year":"2018","journal-title":"Methods Mol Biol"},{"issue":"1","key":"ppat.1010552.ref017","doi-asserted-by":"crossref","first-page":"15931","DOI":"10.1038\/s41598-017-16130-w","article-title":"Broad-spectrum antiviral agents: secreted phospholipase A2 targets viral envelope lipid bilayers derived from the endoplasmic reticulum membrane","volume":"7","author":"M Chen","year":"2017","journal-title":"Sci Rep."},{"issue":"11","key":"ppat.1010552.ref018","doi-asserted-by":"crossref","first-page":"e112351","DOI":"10.1371\/journal.pone.0112351","article-title":"Phospholipase A2 isolated from the venom of Crotalus durissus terrificus inactivates dengue virus and other enveloped viruses by disrupting the viral envelope","volume":"9","author":"VD Muller","year":"2014","journal-title":"PLoS One"},{"issue":"1","key":"ppat.1010552.ref019","doi-asserted-by":"crossref","first-page":"13","DOI":"10.1038\/s41594-017-0010-8","article-title":"Structural biology of Zika virus and other flaviviruses","volume":"25","author":"SS Hasan","year":"2018","journal-title":"Nat Struct Mol Biol"},{"issue":"1","key":"ppat.1010552.ref020","doi-asserted-by":"crossref","first-page":"152","DOI":"10.1016\/j.virol.2010.08.011","article-title":"Lipoprotein lipase and hepatic triglyceride lipase reduce the infectivity of hepatitis C virus (HCV) through their catalytic activities on HCV-associated lipoproteins","volume":"407","author":"Y Shimizu","year":"2010","journal-title":"Virology"},{"issue":"3","key":"ppat.1010552.ref021","doi-asserted-by":"crossref","first-page":"1444","DOI":"10.1128\/JVI.01790-06","article-title":"Lysis of human immunodeficiency virus type 1 by a specific secreted human phospholipase A2","volume":"81","author":"JO Kim","year":"2007","journal-title":"J Virol"},{"issue":"5","key":"ppat.1010552.ref022","doi-asserted-by":"crossref","first-page":"611","DOI":"10.1172\/JCI6915","article-title":"Secreted phospholipases A(2), a new class of HIV inhibitors that block virus entry into host cells","volume":"104","author":"D Fenard","year":"1999","journal-title":"J Clin Invest"},{"issue":"12","key":"ppat.1010552.ref023","doi-asserted-by":"crossref","first-page":"2365","DOI":"10.1016\/j.biochi.2013.08.028","article-title":"Biochemical, functional, structural and phylogenetic studies on Intercro, a new isoform phospholipase A2 from Crotalus durissus terrificus snake venom","volume":"95","author":"LF Vieira","year":"2013","journal-title":"Biochimie"},{"issue":"10","key":"ppat.1010552.ref024","doi-asserted-by":"crossref","first-page":"1780","DOI":"10.3390\/toxins5101780","article-title":"Molecular characterization of Lys49 and Asp49 phospholipases A(2)from snake venom and their antiviral activities against Dengue virus","volume":"5","author":"AB Cecilio","year":"2013","journal-title":"Toxins (Basel)"},{"issue":"7","key":"ppat.1010552.ref025","doi-asserted-by":"crossref","first-page":"1248","DOI":"10.1194\/jlr.R058123","article-title":"A new era of secreted phospholipase A(2)","volume":"56","author":"M Murakami","year":"2015","journal-title":"J Lipid Res"},{"issue":"7","key":"ppat.1010552.ref026","doi-asserted-by":"crossref","first-page":"3274","DOI":"10.1073\/pnas.94.7.3274","article-title":"Prevention of insect-borne disease: An approach using transgenic symbiotic bacteria","volume":"94","author":"RV Durvasula","year":"1997","journal-title":"P Natl Acad Sci USA"},{"issue":"1","key":"ppat.1010552.ref027","doi-asserted-by":"crossref","first-page":"4127","DOI":"10.1038\/s41467-018-06580-9","article-title":"Blood meal-induced inhibition of vector-borne disease by transgenic microbiota","volume":"9","author":"JL Shane","year":"2018","journal-title":"Nat Commun"},{"issue":"31","key":"ppat.1010552.ref028","doi-asserted-by":"crossref","first-page":"12734","DOI":"10.1073\/pnas.1204158109","article-title":"Fighting malaria with engineered symbiotic bacteria from vector mosquitoes","volume":"109","author":"S Wang","year":"2012","journal-title":"Proc Natl Acad Sci U S A"},{"issue":"5","key":"ppat.1010552.ref029","doi-asserted-by":"crossref","first-page":"714","DOI":"10.1016\/j.cell.2010.07.038","article-title":"A C-Type Lectin Collaborates with a CD45 Phosphatase Homolog to Facilitate West Nile Virus Infection of Mosquitoes","volume":"142","author":"G Cheng","year":"2010","journal-title":"Cell"},{"issue":"4","key":"ppat.1010552.ref030","doi-asserted-by":"crossref","first-page":"2047","DOI":"10.1128\/JVI.01840-06","article-title":"Antiviral peptides targeting the west nile virus envelope protein","volume":"81","author":"FW Bai","year":"2007","journal-title":"J Virol"},{"key":"ppat.1010552.ref031","first-page":"6","article-title":"Identification of the Midgut Microbiota of An. stephensi and An. maculipennis for Their Application as a Paratransgenic Tool against Malaria","author":"ND Djadid","year":"2011","journal-title":"Plos One"},{"issue":"3","key":"ppat.1010552.ref032","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pntd.0001561","article-title":"Reciprocal Tripartite Interactions between the Aedes aegypti Midgut Microbiota, Innate Immune System and Dengue Virus Influences Vector Competence","volume":"6","author":"JL Ramirez","year":"2012","journal-title":"Plos Neglect Trop D"},{"issue":"4","key":"ppat.1010552.ref033","doi-asserted-by":"crossref","first-page":"587","DOI":"10.1093\/bioinformatics\/btu661","article-title":"A5-miseq: an updated pipeline to assemble microbial genomes from Illumina MiSeq data","volume":"31","author":"D Coil","year":"2015","journal-title":"Bioinformatics"},{"issue":"5","key":"ppat.1010552.ref034","doi-asserted-by":"crossref","first-page":"722","DOI":"10.1101\/gr.215087.116","article-title":"Canu: scalable and accurate long-read assembly via adaptive k-mer weighting and repeat separation","volume":"27","author":"S Koren","year":"2017","journal-title":"Genome Res"},{"issue":"14","key":"ppat.1010552.ref035","doi-asserted-by":"crossref","first-page":"2103","DOI":"10.1093\/bioinformatics\/btw152","article-title":"miniasm: fast mapping and de novo assembly for noisy long sequences","volume":"32","author":"Li H. Minimap","year":"2016","journal-title":"Bioinformatics"},{"issue":"5","key":"ppat.1010552.ref036","doi-asserted-by":"crossref","first-page":"737","DOI":"10.1101\/gr.214270.116","article-title":"Fast and accurate de novo genome assembly from long uncorrected reads","volume":"27","author":"R Vaser","year":"2017","journal-title":"Genome Res"},{"key":"ppat.1010552.ref037","doi-asserted-by":"crossref","first-page":"294","DOI":"10.1186\/s13059-015-0849-0","article-title":"Circlator: automated circularization of genome assemblies using long sequencing reads","volume":"16","author":"M Hunt","year":"2015","journal-title":"Genome Biol"},{"issue":"11","key":"ppat.1010552.ref038","doi-asserted-by":"crossref","first-page":"e112963","DOI":"10.1371\/journal.pone.0112963","article-title":"Pilon: an integrated tool for comprehensive microbial variant detection and genome assembly improvement","volume":"9","author":"BJ Walker","year":"2014","journal-title":"PLoS One"},{"issue":"6","key":"ppat.1010552.ref039","doi-asserted-by":"crossref","first-page":"1037","DOI":"10.1093\/bioinformatics\/btx713","article-title":"DFAST: a flexible prokaryotic genome annotation pipeline for faster genome publication","volume":"34","author":"Y Tanizawa","year":"2018","journal-title":"Bioinformatics"},{"issue":"6","key":"ppat.1010552.ref040","doi-asserted-by":"crossref","first-page":"413","DOI":"10.1111\/j.1472-765X.1996.tb01192.x","article-title":"Pathogenicity of different isolates of Vibrio harveyi in tiger prawn, Penaeus monodon","volume":"22","author":"PC Liu","year":"1996","journal-title":"Lett Appl Microbiol"},{"key":"ppat.1010552.ref041","doi-asserted-by":"crossref","first-page":"41","DOI":"10.1007\/978-1-4939-0348-1_4","article-title":"Dengue virus purification and sample preparation for cryo-electron microscopy","volume":"1138","author":"JL Tan","year":"2014","journal-title":"Methods Mol Biol"}],"container-title":["PLOS Pathogens"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/dx.plos.org\/10.1371\/journal.ppat.1010552","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,6,9]],"date-time":"2022-06-09T17:29:53Z","timestamp":1654795793000},"score":1,"resource":{"primary":{"URL":"https:\/\/dx.plos.org\/10.1371\/journal.ppat.1010552"}},"subtitle":[],"editor":[{"given":"Richard J.","family":"Kuhn","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2022,6,9]]},"references-count":41,"journal-issue":{"issue":"6","published-online":{"date-parts":[[2022,6,9]]}},"URL":"https:\/\/doi.org\/10.1371\/journal.ppat.1010552","relation":{},"ISSN":["1553-7374"],"issn-type":[{"value":"1553-7374","type":"electronic"}],"subject":[],"published":{"date-parts":[[2022,6,9]]}}}