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Currently, there are no approved treatments or vaccines to combat POWV infection. Here, we generated and characterized a nanoparticle immunogen displaying domain III (EDIII) of the POWV E glycoprotein. Immunization with POWV EDIII presented on nanoparticles resulted in significantly higher serum neutralizing titers against POWV than immunization with monomeric POWV EDIII. Furthermore, passive transfer of EDIII-reactive sera protected against POWV challenge<jats:italic>in vivo<\/jats:italic>. We isolated and characterized a panel of EDIII-specific monoclonal antibodies (mAbs) and identified several that potently inhibit POWV infection and engage distinct epitopes within the lateral ridge and C-C\u2032 loop of the EDIII. By creating a subunit-based nanoparticle immunogen with vaccine potential that elicits antibodies with protective activity against POWV infection, our findings enhance our understanding of the molecular determinants of antibody-mediated neutralization of TBFVs.<\/jats:p>","DOI":"10.1371\/journal.ppat.1010573","type":"journal-article","created":{"date-parts":[[2022,6,9]],"date-time":"2022-06-09T17:48:38Z","timestamp":1654796918000},"page":"e1010573","update-policy":"https:\/\/doi.org\/10.1371\/journal.ppat.corrections_policy","source":"Crossref","is-referenced-by-count":24,"title":["A Powassan virus domain III nanoparticle immunogen elicits neutralizing and protective antibodies in mice"],"prefix":"10.1371","volume":"18","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-5759-3180","authenticated-orcid":true,"given":"Ryan J.","family":"Malonis","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"George I.","family":"Georgiev","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2727-0468","authenticated-orcid":true,"given":"Denise","family":"Haslwanter","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8922-8946","authenticated-orcid":true,"given":"Laura A.","family":"VanBlargan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9355-5501","authenticated-orcid":true,"given":"Georgia","family":"Fallon","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Olivia","family":"Vergnolle","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2877-0704","authenticated-orcid":true,"given":"Sean 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Zhang","year":"2017","journal-title":"Viruses"},{"issue":"1","key":"ppat.1010573.ref023","doi-asserted-by":"crossref","first-page":"3","DOI":"10.1016\/j.coviro.2012.12.001","article-title":"Flaviviruses: braking the entering","volume":"3","author":"TC Pierson","year":"2013","journal-title":"Current opinion in virology"},{"issue":"1","key":"ppat.1010573.ref024","doi-asserted-by":"crossref","first-page":"8449","DOI":"10.1038\/s41598-018-26800-y","article-title":"Molecular basis for dengue virus broad cross-neutralization by humanized monoclonal antibody 513","volume":"8","author":"YH Wong","year":"2018","journal-title":"Scientific Reports"},{"issue":"4","key":"ppat.1010573.ref025","doi-asserted-by":"crossref","first-page":"1016","DOI":"10.1016\/j.cell.2016.07.020","article-title":"Structural Basis of Zika Virus-Specific Antibody Protection","volume":"166","author":"H Zhao","year":"2016","journal-title":"Cell"},{"issue":"5","key":"ppat.1010573.ref026","doi-asserted-by":"crossref","DOI":"10.1084\/jem.20210236","article-title":"Broad and potent neutralizing human antibodies to tick-borne flaviviruses protect mice from disease","volume":"218","author":"M Agudelo","year":"2021","journal-title":"J Exp Med"},{"issue":"7059","key":"ppat.1010573.ref027","doi-asserted-by":"crossref","first-page":"764","DOI":"10.1038\/nature03956","article-title":"Structural basis of West Nile virus neutralization by a therapeutic antibody","volume":"437","author":"GE Nybakken","year":"2005","journal-title":"Nature"},{"key":"ppat.1010573.ref028","doi-asserted-by":"crossref","first-page":"621043","DOI":"10.3389\/fimmu.2020.621043","article-title":"Landscape of Monoclonal Antibodies Targeting Zika and Dengue: Therapeutic Solutions and Critical Insights for Vaccine Development","volume":"11","author":"V Dussupt","year":"2020","journal-title":"Frontiers in 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Hermida","year":"2006","journal-title":"Vaccine"},{"issue":"17","key":"ppat.1010573.ref038","article-title":"Rational Design of Zika Virus Subunit Vaccine with Enhanced Efficacy","volume":"93","author":"W Tai","year":"2019","journal-title":"J Virol"},{"issue":"3","key":"ppat.1010573.ref039","doi-asserted-by":"crossref","DOI":"10.3390\/vaccines7030072","article-title":"Zika Virus-Derived E-DIII Protein Displayed on Immunologically Optimized VLPs Induces Neutralizing Antibodies without Causing Enhancement of Dengue Virus Infection.","volume":"7","author":"G Cabral-Miranda","year":"2019","journal-title":"Vaccines"},{"key":"ppat.1010573.ref040","article-title":"Resurfaced ZIKV EDIII nanoparticle immunogens elicit neutralizing and protective responses in vivo","author":"GI Georgiev","year":"2022","journal-title":"Cell Chem Biol"},{"issue":"6","key":"ppat.1010573.ref041","doi-asserted-by":"crossref","first-page":"3210","DOI":"10.1021\/acs.chemrev.9b00472","article-title":"Peptide-Based 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at 1.6 A resolution: determinants of thermostability revealed from structural comparisons","volume":"306","author":"X Zhang","year":"2001","journal-title":"Journal of molecular biology"},{"issue":"1","key":"ppat.1010573.ref057","doi-asserted-by":"crossref","first-page":"53","DOI":"10.1016\/j.virol.2005.10.030","article-title":"A rapid and quantitative assay for measuring antibody-mediated neutralization of West Nile virus infection","volume":"346","author":"TC Pierson","year":"2006","journal-title":"Virology"},{"issue":"1","key":"ppat.1010573.ref058","doi-asserted-by":"crossref","first-page":"544","DOI":"10.1186\/s12879-016-1884-5","article-title":"Recombinant domains III of Tick-Borne Encephalitis Virus envelope protein in combination with dextran and CpGs induce immune response and partial protectiveness against TBE virus infection in mice","volume":"16","author":"AS Ershova","year":"2016","journal-title":"BMC Infectious 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Kato","year":"2020","journal-title":"Immunity"},{"issue":"20","key":"ppat.1010573.ref062","doi-asserted-by":"crossref","first-page":"11726","DOI":"10.1128\/JVI.01140-14","article-title":"Combined effects of the structural heterogeneity and dynamics of flaviviruses on antibody recognition","volume":"88","author":"KA Dowd","year":"2014","journal-title":"Journal of virology"},{"issue":"1","key":"ppat.1010573.ref063","doi-asserted-by":"crossref","first-page":"185","DOI":"10.1146\/annurev-virology-092917-043300","article-title":"The Many Faces of a Dynamic Virion: Implications of Viral Breathing on Flavivirus Biology and Immunogenicity","volume":"5","author":"KA Dowd","year":"2018","journal-title":"Annual review of virology"},{"issue":"4","key":"ppat.1010573.ref064","doi-asserted-by":"crossref","first-page":"570","DOI":"10.3201\/eid0504.990423","article-title":"A focus of deer tick virus transmission in the northcentral United States","volume":"5","author":"GD Ebel","year":"1999","journal-title":"Emerg Infect Dis"},{"issue":"6","key":"ppat.1010573.ref065","doi-asserted-by":"crossref","first-page":"971","DOI":"10.4269\/ajtmh.2008.79.971","article-title":"Stable Prevalence of Powassan Virus in Ixodes scapularis in a Northern Wisconsin Focus.","volume":"79","author":"DE Brackney","year":"2008","journal-title":"The American Journal of Tropical Medicine and Hygiene Am J Trop Med Hyg"},{"issue":"11","key":"ppat.1010573.ref066","doi-asserted-by":"crossref","first-page":"e1008061","DOI":"10.1371\/journal.ppat.1008061","article-title":"Human monoclonal antibodies against chikungunya virus target multiple distinct epitopes in the E1 and E2 glycoproteins","volume":"15","author":"JA Quiroz","year":"2019","journal-title":"PLoS Pathog"},{"issue":"10","key":"ppat.1010573.ref067","doi-asserted-by":"crossref","first-page":"1908","DOI":"10.1038\/nprot.2016.102","article-title":"Sequencing and cloning of antigen-specific antibodies from mouse memory B cells","volume":"11","author":"L von Boehmer","year":"2016","journal-title":"Nature protocols"},{"issue":"37","key":"ppat.1010573.ref068","doi-asserted-by":"crossref","DOI":"10.1073\/pnas.2100104118","article-title":"Near-germline human monoclonal antibodies neutralize and protect against multiple arthritogenic alphaviruses","volume":"118","author":"RJ Malonis","year":"2021","journal-title":"Proc Natl Acad Sci U S A"},{"issue":"1\u20132","key":"ppat.1010573.ref069","doi-asserted-by":"crossref","first-page":"41","DOI":"10.1016\/j.jim.2007.01.008","article-title":"Antibody internalization studied using a novel IgG binding toxin fusion","volume":"321","author":"Y Mazor","year":"2007","journal-title":"Journal of immunological methods"},{"key":"ppat.1010573.ref070","doi-asserted-by":"crossref","first-page":"D1006","DOI":"10.1093\/nar\/gkn838","article-title":"IMGT, the international ImMunoGeneTics information system","volume":"37","author":"MP Lefranc","year":"2009","journal-title":"Nucleic acids research"},{"key":"ppat.1010573.ref071","volume-title":"Protein NMR Spectroscopy: Principles and Practice","author":"J Cavanagh","year":"2010"},{"issue":"8","key":"ppat.1010573.ref072","doi-asserted-by":"crossref","first-page":"1529","DOI":"10.1016\/j.bpj.2017.03.011","article-title":"NMRbox: A Resource for Biomolecular NMR Computation","volume":"112","author":"MW Maciejewski","year":"2017","journal-title":"Biophys J"},{"issue":"3","key":"ppat.1010573.ref073","doi-asserted-by":"crossref","first-page":"277","DOI":"10.1007\/BF00197809","article-title":"NMRPipe: a multidimensional spectral processing system based on UNIX pipes","volume":"6","author":"F Delaglio","year":"1995","journal-title":"J Biomol NMR"},{"issue":"2","key":"ppat.1010573.ref074","doi-asserted-by":"crossref","first-page":"111","DOI":"10.1007\/s10858-016-0060-y","article-title":"CcpNmr AnalysisAssign: a flexible platform for integrated NMR analysis","volume":"66","author":"SP Skinner","year":"2016","journal-title":"J Biomol NMR"},{"issue":"1","key":"ppat.1010573.ref075","doi-asserted-by":"crossref","first-page":"117","DOI":"10.1351\/pac199870010117","article-title":"Recommendations for the presentation of NMR structures of proteins and nucleic acids (IUPAC Recommendations 1998).","volume":"70","author":"JL Markley","year":"1998","journal-title":"Pure and applied chemistry"},{"issue":"4","key":"ppat.1010573.ref076","doi-asserted-by":"crossref","first-page":"425","DOI":"10.1007\/s10858-015-9928-5","article-title":"Improved reliability, accuracy and quality in automated NMR structure calculation with ARIA","volume":"62","author":"F Mareuil","year":"2015","journal-title":"J Biomol NMR"},{"issue":"Pt 5","key":"ppat.1010573.ref077","doi-asserted-by":"crossref","first-page":"905","DOI":"10.1107\/S0907444998003254","article-title":"Crystallography & NMR system: A new software suite for macromolecular structure determination","volume":"54","author":"AT 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