{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,13]],"date-time":"2026-10-13T01:25:30Z","timestamp":1791854730152,"version":"4.3.4"},"update-to":[{"DOI":"10.1371\/journal.ppat.1010883","type":"new_version","label":"New version","source":"publisher","updated":{"date-parts":[[2023,4,17]],"date-time":"2023-04-17T00:00:00Z","timestamp":1681689600000}}],"reference-count":75,"publisher":"Public Library of Science (PLoS)","issue":"3","license":[{"start":{"date-parts":[[2023,3,30]],"date-time":"2023-03-30T00:00:00Z","timestamp":1680134400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100000275","name":"Leverhulme Trust","doi-asserted-by":"publisher","award":["RPG-2020-236"],"award-info":[{"award-number":["RPG-2020-236"]}],"id":[{"id":"10.13039\/501100000275","id-type":"DOI","asserted-by":"publisher"}]},{"name":"The National Secretariat of Science, Technology and Innovation of the Republic of Panama","award":["Studentship"],"award-info":[{"award-number":["Studentship"]}]},{"DOI":"10.13039\/501100001807","name":"Funda\u00e7\u00e3o de Amparo \u00e0 Pesquisa do Estado de S\u00e3o Paulo","doi-asserted-by":"publisher","award":["2019\/03997-2"],"award-info":[{"award-number":["2019\/03997-2"]}],"id":[{"id":"10.13039\/501100001807","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001807","name":"Funda\u00e7\u00e3o de Amparo \u00e0 Pesquisa do Estado de S\u00e3o Paulo","doi-asserted-by":"publisher","award":["2021\/13166-0"],"award-info":[{"award-number":["2021\/13166-0"]}],"id":[{"id":"10.13039\/501100001807","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["www.plospathogens.org"],"crossmark-restriction":false},"short-container-title":["PLoS Pathog"],"abstract":"<jats:p>Metagenomic studies have demonstrated that viruses are extremely diverse and abundant in insects, but the difficulty of isolating them means little is known about the biology of these newly discovered viruses. To overcome this challenge in <jats:italic>Drosophila<\/jats:italic>, we created a cell line that was more permissive to infection and detected novel viruses by the presence of double-stranded RNA. We demonstrate the utility of these tools by isolating La Jolla virus (LJV) and Newfield virus (NFV) from several wild <jats:italic>Drosophila<\/jats:italic> populations. These viruses have different potential host ranges, with distinct abilities to replicate in five <jats:italic>Drosophila<\/jats:italic> species. Similarly, in some species they cause high mortality and in others they are comparatively benign. In three species, NFV but not LJV caused large declines in female fecundity. This sterilization effect was associated with differences in tissue tropism, as NFV but not LJV was able to infect <jats:italic>Drosophila melanogaster<\/jats:italic> follicular epithelium and induce follicular degeneration in the ovary. We saw a similar effect in the invasive pest of fruit crops <jats:italic>Drosophila suzukii<\/jats:italic>, where oral infection with NFV caused reductions in the fecundity, suggesting it has potential as a biocontrol agent. In conclusion, a simple protocol allowed us to isolate new viruses and demonstrate that viruses identified by metagenomics have a large effect on the fitness of the model organism <jats:italic>D<\/jats:italic>. <jats:italic>melanogaster<\/jats:italic> and related species.<\/jats:p>","DOI":"10.1371\/journal.ppat.1010883","type":"journal-article","created":{"date-parts":[[2023,3,30]],"date-time":"2023-03-30T17:57:13Z","timestamp":1680199033000},"page":"e1010883","update-policy":"https:\/\/doi.org\/10.1371\/journal.ppat.corrections_policy","source":"Crossref","is-referenced-by-count":18,"title":["Hunting Drosophila viruses from wild populations: A novel isolation approach and characterisation of viruses"],"prefix":"10.1371","volume":"19","author":[{"given":"Gaspar","family":"Bruner-Montero","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Carlos M.","family":"Luque","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"C\u00e1ssia Siqueira","family":"Cesar","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shuai Dominique","family":"Ding","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jonathan P.","family":"Day","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7470-8157","authenticated-orcid":true,"given":"Francis Michael","family":"Jiggins","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"340","published-online":{"date-parts":[[2023,3,30]]},"reference":[{"key":"ppat.1010883.ref001","doi-asserted-by":"crossref","first-page":"119","DOI":"10.1146\/annurev-virology-092818-015851","article-title":"Expanding the RNA virosphere by unbiased metagenomics","volume":"6","author":"Y-Z Zhang","year":"2019","journal-title":"Annual Review of Virology"},{"key":"ppat.1010883.ref002","unstructured":"Obbard DJ. A comprehensive list of published Drosophila viruses (excluding retroelements) https:\/\/obbard.bio.ed.ac.uk\/data.html2022 [cited 2022]."},{"issue":"1","key":"ppat.1010883.ref003","doi-asserted-by":"crossref","DOI":"10.1093\/ve\/veab031","article-title":"The discovery, distribution, and diversity of DNA viruses associated with Drosophila melanogaster in Europe","volume":"7","author":"MA Wallace","year":"2021","journal-title":"Virus Evolution"},{"issue":"Suppl 2","key":"ppat.1010883.ref004","first-page":"13","article-title":"Twenty-Five New Viruses Associated with the Drosophilidae (Diptera).","volume":"12","author":"CL Webster","year":"2016","journal-title":"Evol Bioinform Online."},{"issue":"7","key":"ppat.1010883.ref005","doi-asserted-by":"crossref","first-page":"e1002210","DOI":"10.1371\/journal.pbio.1002210","article-title":"The discovery, distribution, and evolution of viruses associated with Drosophila melanogaster","volume":"13","author":"CL Webster","year":"2015","journal-title":"PLoS biology"},{"key":"ppat.1010883.ref006","doi-asserted-by":"crossref","first-page":"74","DOI":"10.1016\/j.jip.2018.04.001","article-title":"Identification and characterization of natural viruses associated with the invasive insect pest Drosophila suzukii","volume":"154","author":"T Carrau","year":"2018","journal-title":"Journal of invertebrate pathology"},{"key":"ppat.1010883.ref007","article-title":"Galbut virus infection minimally influences Drosophila melanogaster fitness traits in a strain and sex-dependent manner.","author":"ST Cross","year":"2021","journal-title":"bioRxiv"},{"issue":"6","key":"ppat.1010883.ref008","doi-asserted-by":"crossref","first-page":"e1007050","DOI":"10.1371\/journal.ppat.1007050","article-title":"Isolation of a natural DNA virus of Drosophila melanogaster, and characterisation of host resistance and immune responses.","volume":"14","author":"WH Palmer","year":"2018","journal-title":"PLoS Pathogens."},{"key":"ppat.1010883.ref009","first-page":"625","author":"G Brun","year":"1980"},{"issue":"1","key":"ppat.1010883.ref010","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1038\/s42003-021-02838-z","article-title":"Wolbachia reduces virus infection in a natural population of Drosophila","volume":"4","author":"R Cogni","year":"2021","journal-title":"Communications Biology"},{"issue":"20","key":"ppat.1010883.ref011","doi-asserted-by":"crossref","first-page":"e01070","DOI":"10.1128\/JVI.01070-20","article-title":"Partitiviruses infecting Drosophila melanogaster and Aedes aegypti exhibit efficient biparental vertical transmission","volume":"94","author":"ST Cross","year":"2020","journal-title":"Journal of Virology"},{"issue":"8","key":"ppat.1010883.ref012","doi-asserted-by":"crossref","first-page":"e12421","DOI":"10.1371\/journal.pone.0012421","article-title":"Host range and specificity of the Drosophila C virus.","volume":"5","author":"M Kapun","year":"2010","journal-title":"PLoS One"},{"issue":"29","key":"ppat.1010883.ref013","doi-asserted-by":"crossref","first-page":"e2122026119","DOI":"10.1073\/pnas.2122026119","article-title":"A novel transposable element-mediated mechanism causes antiviral resistance in Drosophila through truncating the Veneno protein","volume":"119","author":"O Brosh","year":"2022","journal-title":"Proceedings of the National Academy of Sciences"},{"issue":"10","key":"ppat.1010883.ref014","doi-asserted-by":"crossref","first-page":"e26564","DOI":"10.1371\/journal.pone.0026564","article-title":"A DNA virus of Drosophila","volume":"6","author":"RL Unckless","year":"2011","journal-title":"PloS one"},{"issue":"3","key":"ppat.1010883.ref015","doi-asserted-by":"crossref","first-page":"261","DOI":"10.1007\/BF01317341","article-title":"Comparison of various physiological traits in flies (Drosophila melanogaster) of wild origin, infected or uninfected by the hereditary rhabdovirus sigma","volume":"69","author":"A. Fleuriet","year":"1981","journal-title":"Archives of Virology"},{"issue":"3","key":"ppat.1010883.ref016","doi-asserted-by":"crossref","first-page":"761","DOI":"10.1111\/j.1558-5646.2012.01832.x","article-title":"The dynamics of reciprocal selective sweeps of host resistance and a parasite counter-adaptation in Drosophila.","volume":"67","author":"L Wilfert","year":"2013","journal-title":"Evolution: International Journal of Organic Evolution."},{"issue":"6","key":"ppat.1010883.ref017","doi-asserted-by":"crossref","first-page":"580","DOI":"10.1016\/j.cub.2006.01.065","article-title":"Natural selection drives extremely rapid evolution in antiviral RNAi genes","volume":"16","author":"DJ Obbard","year":"2006","journal-title":"Current biology"},{"issue":"1621","key":"ppat.1010883.ref018","doi-asserted-by":"crossref","first-page":"2027","DOI":"10.1098\/rspb.2007.0611","article-title":"The age and evolution of an antiviral resistance mutation in Drosophila melanogaster.","volume":"274","author":"J Bangham","year":"2007","journal-title":"Proceedings of the Royal Society B: Biological Sciences."},{"issue":"10","key":"ppat.1010883.ref019","doi-asserted-by":"crossref","first-page":"e1002337","DOI":"10.1371\/journal.pgen.1002337","article-title":"Successive increases in the resistance of Drosophila to viral infection through a transposon insertion followed by a duplication","volume":"7","author":"MM Magwire","year":"2011","journal-title":"PLoS genetics"},{"key":"ppat.1010883.ref020","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.jip.2015.07.009","article-title":"Insect pathogens as biological control agents: back to the future","volume":"132","author":"L Lacey","year":"2015","journal-title":"Journal of invertebrate pathology"},{"issue":"6","key":"ppat.1010883.ref021","doi-asserted-by":"crossref","first-page":"1839","DOI":"10.1093\/jee\/98.6.1839","article-title":"Effective control of a field population of Helicoverpa armigera by using the small RNA virus Helicoverpa armigera stunt virus (Tetraviridae: Omegatetravirus).","volume":"98","author":"PD Christian","year":"2005","journal-title":"Journal of economic entomology"},{"issue":"3","key":"ppat.1010883.ref022","doi-asserted-by":"crossref","first-page":"e0003629","DOI":"10.1371\/journal.pntd.0003629","article-title":"Viral RNA intermediates as targets for detection and discovery of novel and emerging mosquito-borne viruses","volume":"9","author":"CA O\u2019Brien","year":"2015","journal-title":"PLoS neglected tropical diseases"},{"issue":"5571","key":"ppat.1010883.ref023","doi-asserted-by":"crossref","first-page":"1319","DOI":"10.1126\/science.1070948","article-title":"Induction and suppression of RNA silencing by an animal virus","volume":"296","author":"H Li","year":"2002","journal-title":"Science"},{"issue":"6","key":"ppat.1010883.ref024","doi-asserted-by":"crossref","first-page":"1066","DOI":"10.3390\/v13061066","article-title":"An Aedes aegypti-derived Ago2 knockout cell line to investigate arbovirus infections","volume":"13","author":"C Scherer","year":"2021","journal-title":"Viruses"},{"issue":"7053","key":"ppat.1010883.ref025","doi-asserted-by":"crossref","first-page":"1040","DOI":"10.1038\/nature03870","article-title":"Animal virus replication and RNAi-mediated antiviral silencing in Caenorhabditis elegans","volume":"436","author":"R Lu","year":"2005","journal-title":"Nature"},{"issue":"10","key":"ppat.1010883.ref026","doi-asserted-by":"crossref","first-page":"5059","DOI":"10.1128\/JVI.80.10.5059-5064.2006","article-title":"Double-stranded RNA is produced by positive-strand RNA viruses and DNA viruses but not in detectable amounts by negative-strand RNA viruses","volume":"80","author":"F Weber","year":"2006","journal-title":"Journal of virology"},{"issue":"4","key":"ppat.1010883.ref027","doi-asserted-by":"crossref","first-page":"1606","DOI":"10.1073\/pnas.0911353107","article-title":"Virus discovery by deep sequencing and assembly of virus-derived small silencing RNAs","volume":"107","author":"Q Wu","year":"2010","journal-title":"Proceedings of the National Academy of Sciences"},{"issue":"7634","key":"ppat.1010883.ref028","doi-asserted-by":"crossref","first-page":"539","DOI":"10.1038\/nature20167","article-title":"Redefining the invertebrate RNA virosphere","volume":"540","author":"M Shi","year":"2016","journal-title":"Nature"},{"issue":"5","key":"ppat.1010883.ref029","doi-asserted-by":"crossref","first-page":"740","DOI":"10.3390\/v13050740","article-title":"Organization of the structural protein region of La Jolla Virus isolated from the invasive pest insect Drosophila suzukii.","volume":"13","author":"T Carrau","year":"2021","journal-title":"Viruses"},{"issue":"12","key":"ppat.1010883.ref030","doi-asserted-by":"crossref","first-page":"e1005265","DOI":"10.1371\/journal.ppat.1005265","article-title":"The structure of the RNA-dependent RNA polymerase of a permutotetravirus suggests a link between primer-dependent and primer-independent polymerases","volume":"11","author":"DS Ferrero","year":"2015","journal-title":"PLoS pathogens"},{"issue":"12","key":"ppat.1010883.ref031","first-page":"13","article-title":"Twenty-five new viruses associated with the Drosophilidae (Diptera).","volume":"12","author":"CL Webster","year":"2016","journal-title":"Evol Bioinform."},{"issue":"1883","key":"ppat.1010883.ref032","doi-asserted-by":"crossref","first-page":"20181165","DOI":"10.1098\/rspb.2018.1165","article-title":"No detectable effect of Wolbachia w Mel on the prevalence and abundance of the RNA virome of Drosophila melanogaster.","volume":"285","author":"M Shi","year":"2018","journal-title":"Proceedings of the Royal Society B: Biological Sciences."},{"issue":"1","key":"ppat.1010883.ref033","first-page":"vey009","article-title":"The virome of Drosophila suzukii, an invasive pest of soft fruit","volume":"4","author":"NC Medd","year":"2018","journal-title":"Virus evolution"},{"issue":"9","key":"ppat.1010883.ref034","doi-asserted-by":"crossref","first-page":"929","DOI":"10.3390\/v12090929","article-title":"Viral metagenomic analysis of Aedes albopictus mosquitos from southern Switzerland.","volume":"12","author":"J Kubacki","year":"2020","journal-title":"Viruses"},{"key":"ppat.1010883.ref035","doi-asserted-by":"crossref","first-page":"e68353","DOI":"10.7554\/eLife.68353","article-title":"Single mosquito metatranscriptomics identifies vectors, emerging pathogens and reservoirs in one assay.","volume":"10","author":"J Batson","year":"2021","journal-title":"Elife"},{"issue":"3","key":"ppat.1010883.ref036","doi-asserted-by":"crossref","first-page":"799","DOI":"10.1007\/s00705-016-3158-3","article-title":"Genome sequence, prevalence and quantification of the first iflavirus identified in a phytoplasma insect vector","volume":"162","author":"S Abb\u00e0","year":"2017","journal-title":"Archives of virology"},{"issue":"5","key":"ppat.1010883.ref037","doi-asserted-by":"crossref","first-page":"575","DOI":"10.3390\/v12050575","article-title":"Tolerance of honey bees to Varroa mite in the absence of deformed wing virus.","volume":"12","author":"JM Roberts","year":"2020","journal-title":"Viruses"},{"issue":"3","key":"ppat.1010883.ref038","doi-asserted-by":"crossref","first-page":"368","DOI":"10.1007\/s11262-019-01652-4","article-title":"Nine new RNA viruses associated with the fire ant Solenopsis invicta from its native range","volume":"55","author":"SM Valles","year":"2019","journal-title":"Virus Genes"},{"issue":"11","key":"ppat.1010883.ref039","doi-asserted-by":"crossref","first-page":"1041","DOI":"10.3390\/v11111041","article-title":"Viruses in the invasive hornet Vespa velutina.","volume":"11","author":"A Dalmon","year":"2019","journal-title":"Viruses"},{"issue":"4","key":"ppat.1010883.ref040","doi-asserted-by":"crossref","first-page":"527","DOI":"10.1099\/jgv.0.000757","article-title":"ICTV virus taxonomy profile: Iflaviridae","volume":"98","author":"S Valles","year":"2017","journal-title":"The Journal of general virology"},{"issue":"11","key":"ppat.1010883.ref041","doi-asserted-by":"crossref","first-page":"1218","DOI":"10.3390\/v12111218","article-title":"Metagenomic approach with the NetoVIR enrichment protocol reveals virus diversity within ethiopian honey bees (Apis mellifera simensis).","volume":"12","author":"H Gebremedhn","year":"2020","journal-title":"Viruses"},{"issue":"6","key":"ppat.1010883.ref042","doi-asserted-by":"crossref","first-page":"818","DOI":"10.1099\/jgv.0.001073","article-title":"Metagenomic analysis of Varroa-free Australian honey bees (Apis mellifera) shows a diverse Picornavirales virome.","volume":"99","author":"JM Roberts","year":"2018","journal-title":"Journal of General Virology"},{"issue":"4","key":"ppat.1010883.ref043","doi-asserted-by":"crossref","first-page":"2261","DOI":"10.1111\/tbed.13878","article-title":"Next generation sequencing study on RNA viruses of Vespa velutina and Apis mellifera sharing the same foraging area","volume":"68","author":"F Marzoli","year":"2021","journal-title":"Transboundary and Emerging Diseases"},{"issue":"3","key":"ppat.1010883.ref044","first-page":"3_117","article-title":"Isolation of the Thosea asigna virus (TaV) from the epizootic Setothosea asigna larvae collected in South Sumatra and a study on its pathogenicity to Limacodidae larvae in Japan.","volume":"79","author":"M Sugiharti","year":"2011","journal-title":"Journal of insect biotechnology and sericology"},{"issue":"2","key":"ppat.1010883.ref045","doi-asserted-by":"crossref","first-page":"147","DOI":"10.3390\/v12020147","article-title":"Entomological assessment of the status and risk of mosquito-borne arboviral transmission in Ghana.","volume":"12","author":"M Amoa-Bosompem","year":"2020","journal-title":"Viruses"},{"issue":"8","key":"ppat.1010883.ref046","doi-asserted-by":"crossref","first-page":"866","DOI":"10.3390\/v12080866","article-title":"Aedes aegypti from Amazon basin harbor high diversity of novel viral species.","volume":"12","author":"GdO Ribeiro","year":"2020","journal-title":"Viruses"},{"issue":"5","key":"ppat.1010883.ref047","doi-asserted-by":"crossref","first-page":"791","DOI":"10.3390\/v13050791","article-title":"Characterisation of the viral community associated with the Alfalfa Weevil (Hypera postica) and its host plant, Alfalfa (Medicago sativa).","volume":"13","author":"S Fran\u00e7ois","year":"2021","journal-title":"Viruses"},{"issue":"3","key":"ppat.1010883.ref048","doi-asserted-by":"crossref","first-page":"287","DOI":"10.3390\/v12030287","article-title":"New viral sequences identified in the Flavescence Dor\u00e9e phytoplasma vector Scaphoideus titanus.","volume":"12","author":"S Ottati","year":"2020","journal-title":"Viruses"},{"issue":"12","key":"ppat.1010883.ref049","doi-asserted-by":"crossref","first-page":"1376","DOI":"10.3390\/v12121376","article-title":"Soybean thrips (Thysanoptera: Thripidae) harbor highly diverse populations of arthropod, fungal and plant viruses.","volume":"12","author":"T Thekke-Veetil","year":"2020","journal-title":"Viruses"},{"key":"ppat.1010883.ref050","doi-asserted-by":"crossref","first-page":"49","DOI":"10.1016\/j.virusres.2017.08.012","article-title":"Genomes of viral isolates derived from different mosquitos species","volume":"242","author":"M Sadeghi","year":"2017","journal-title":"Virus research"},{"issue":"8","key":"ppat.1010883.ref051","doi-asserted-by":"crossref","first-page":"e00736","DOI":"10.1016\/j.heliyon.2018.e00736","article-title":"Persistent viruses in mosquito cultured cell line suppress multiplication of flaviviruses","volume":"4","author":"R Fujita","year":"2018","journal-title":"Heliyon"},{"issue":"6890","key":"ppat.1010883.ref052","doi-asserted-by":"crossref","first-page":"735","DOI":"10.1038\/nature00810","article-title":"Geographic structure and dynamics of coevolutionary selection","volume":"417","author":"JN Thompson","year":"2002","journal-title":"Nature"},{"issue":"7171","key":"ppat.1010883.ref053","doi-asserted-by":"crossref","first-page":"870","DOI":"10.1038\/nature06291","article-title":"Host-parasite \u2019Red Queen\u2019 dynamics archived in pond sediment.","volume":"450","author":"E Decaestecker","year":"2007","journal-title":"Nature"},{"key":"ppat.1010883.ref054","doi-asserted-by":"crossref","DOI":"10.3389\/fpls.2015.01084","article-title":"Epidemiological and evolutionary outcomes in gene-for-gene and matching allele models.","volume":"6","author":"PH Thrall","year":"2016","journal-title":"Front Plant Sci"},{"issue":"7","key":"ppat.1010883.ref055","doi-asserted-by":"crossref","first-page":"e1004256","DOI":"10.1371\/journal.ppat.1004256","article-title":"Novel Drosophila viruses encode host-specific suppressors of RNAi.","volume":"10","author":"JT van Mierlo","year":"2014","journal-title":"PLoS pathogens."},{"key":"ppat.1010883.ref056","doi-asserted-by":"crossref","first-page":"335","DOI":"10.2307\/2407221","article-title":"Host castration as a parasitic strategy","author":"M. Baudoin","year":"1975","journal-title":"Evolution"},{"issue":"5","key":"ppat.1010883.ref057","doi-asserted-by":"crossref","first-page":"472","DOI":"10.1002\/evl3.247","article-title":"Between virus correlations in the outcome of infection across host species: Evidence of virus by host species interactions","volume":"5","author":"RM Imrie","year":"2021","journal-title":"Evolution letters"},{"issue":"3","key":"ppat.1010883.ref058","doi-asserted-by":"crossref","first-page":"e1004728","DOI":"10.1371\/journal.ppat.1004728","article-title":"The causes and consequences of changes in virulence following pathogen host shifts.","volume":"11","author":"B Longdon","year":"2015","journal-title":"PLoS pathogens."},{"issue":"9","key":"ppat.1010883.ref059","doi-asserted-by":"crossref","first-page":"e1002260","DOI":"10.1371\/journal.ppat.1002260","article-title":"Host phylogeny determines viral persistence and replication in novel hosts.","volume":"7","author":"B Longdon","year":"2011","journal-title":"PLoS pathogens."},{"issue":"10","key":"ppat.1010883.ref060","doi-asserted-by":"crossref","first-page":"2158","DOI":"10.3390\/v14102158","article-title":"Pathogenicity of La Jolla Virus in Drosophila suzukii following Oral Administration.","volume":"14","author":"Y Linscheid","year":"2022","journal-title":"Viruses"},{"key":"ppat.1010883.ref061","doi-asserted-by":"crossref","first-page":"138","DOI":"10.1016\/j.jip.2017.06.010","article-title":"Analysis of virus susceptibility in the invasive insect pest Drosophila suzukii","volume":"148","author":"K-Z Lee","year":"2017","journal-title":"Journal of invertebrate pathology"},{"key":"ppat.1010883.ref062","article-title":"A review of the invasion of Drosophila suzukii in Europe and a draft research agenda for integrated pest management.","author":"A Cini","year":"2012"},{"key":"ppat.1010883.ref063","article-title":"A fasta file of known Drosophila virus sequences","author":"DJ Obbard","year":"2022"},{"issue":"8","key":"ppat.1010883.ref064","doi-asserted-by":"crossref","first-page":"1494","DOI":"10.1038\/nprot.2013.084","article-title":"De novo transcript sequence reconstruction from RNA-seq using the Trinity platform for reference generation and analysis","volume":"8","author":"BJ Haas","year":"2013","journal-title":"Nature protocols"},{"issue":"5","key":"ppat.1010883.ref065","doi-asserted-by":"crossref","first-page":"1792","DOI":"10.1093\/nar\/gkh340","article-title":"MUSCLE: multiple sequence alignment with high accuracy and high throughput","volume":"32","author":"RC Edgar","year":"2004","journal-title":"Nucleic Acids Res"},{"issue":"7","key":"ppat.1010883.ref066","doi-asserted-by":"crossref","first-page":"1253","DOI":"10.1093\/molbev\/msn083","article-title":"jModelTest: Phylogenetic model averaging","volume":"25","author":"D. Posada","year":"2008","journal-title":"Mol Biol Evol"},{"issue":"3","key":"ppat.1010883.ref067","doi-asserted-by":"crossref","first-page":"539","DOI":"10.1093\/sysbio\/sys029","article-title":"MrBayes 3.2: efficient bayesian phylogenetic inference and model choice across a large model space","volume":"61","author":"F Ronquist","year":"2012","journal-title":"Syst Biol"},{"issue":"1","key":"ppat.1010883.ref068","doi-asserted-by":"crossref","first-page":"191","DOI":"10.1099\/0022-1317-79-1-191","article-title":"The novel genome organization of the insect picorna-like virus Drosophila C virus suggests this virus belongs to a previously undescribed virus family","volume":"79","author":"KN Johnson","year":"1998","journal-title":"Journal of General Virology"},{"issue":"3","key":"ppat.1010883.ref069","doi-asserted-by":"crossref","first-page":"493","DOI":"10.1093\/oxfordjournals.aje.a118408","article-title":"A simple method of estimating fifty per cent endpoints","volume":"27","author":"LJ Reed","year":"1938","journal-title":"American journal of epidemiology"},{"key":"ppat.1010883.ref070","author":"R Core Team.","year":"2022"},{"key":"ppat.1010883.ref071","author":"H. Wickham","year":"2016"},{"issue":"1","key":"ppat.1010883.ref072","doi-asserted-by":"crossref","first-page":"1","DOI":"10.18637\/jss.v069.i01","article-title":"Least-Squares Means: The R Package lsmeans.","volume":"69","author":"RV Lenth","year":"2016","journal-title":"J Stat Softw."},{"key":"ppat.1010883.ref073","unstructured":"Therneau TM. coxme: mixed effects cox models. R package version 2.2\u20135. URL: http:\/\/CRANR-projectorg\/package=coxme. 2015."},{"issue":"2","key":"ppat.1010883.ref074","doi-asserted-by":"crossref","first-page":"378","DOI":"10.32614\/RJ-2017-066","article-title":"glmmTMB balances speed and flexibility among packages for zero-inflated generalized linear mixed modeling.","volume":"9","author":"ME Brooks","year":"2017","journal-title":"R J"},{"key":"ppat.1010883.ref075","author":"J Fox","year":"2018"}],"updated-by":[{"DOI":"10.1371\/journal.ppat.1010883","type":"new_version","label":"New version","source":"publisher","updated":{"date-parts":[[2023,4,17]],"date-time":"2023-04-17T00:00:00Z","timestamp":1681689600000}}],"container-title":["PLOS Pathogens"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/dx.plos.org\/10.1371\/journal.ppat.1010883","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,4,17]],"date-time":"2023-04-17T17:43:27Z","timestamp":1681753407000},"score":1,"resource":{"primary":{"URL":"https:\/\/dx.plos.org\/10.1371\/journal.ppat.1010883"}},"subtitle":[],"editor":[{"given":"Elizabeth A.","family":"McGraw","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2023,3,30]]},"references-count":75,"journal-issue":{"issue":"3","published-online":{"date-parts":[[2023,3,30]]}},"URL":"https:\/\/doi.org\/10.1371\/journal.ppat.1010883","relation":{"new_version":[{"id-type":"doi","id":"10.1371\/journal.ppat.1010883","asserted-by":"object"}]},"ISSN":["1553-7374"],"issn-type":[{"value":"1553-7374","type":"electronic"}],"subject":[],"published":{"date-parts":[[2023,3,30]]}}}