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To understand the molecular mechanism of MuV infection, a functional analysis of the involved host factors is required. However, little is known about the host factors involved in MuV infection, especially those involved in the late stage of infection. Here, we identified 638 host proteins that have close proximity to MuV glycoproteins, which are a major component of the viral particles, by proximity labeling and examined comprehensive protein\u2013protein interaction networks of the host proteins. From siRNA screening and immunoprecipitation results, we found that a SNARE subfamily protein, USE1, bound specifically to the MuV fusion (F) protein and was important for MuV propagation. In addition, USE1 plays a role in complete N-linked glycosylation and expression of the MuV F protein.<\/jats:p>","DOI":"10.1371\/journal.ppat.1010949","type":"journal-article","created":{"date-parts":[[2022,12,8]],"date-time":"2022-12-08T18:24:01Z","timestamp":1670523841000},"page":"e1010949","update-policy":"https:\/\/doi.org\/10.1371\/journal.ppat.corrections_policy","source":"Crossref","is-referenced-by-count":9,"title":["SNARE protein USE1 is involved in the glycosylation and the expression of mumps virus fusion protein and important for viral propagation"],"prefix":"10.1371","volume":"18","author":[{"given":"Yaqing","family":"Liu","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8613-4717","authenticated-orcid":true,"given":"Hiroshi","family":"Katoh","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tsuyoshi","family":"Sekizuka","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chaewon","family":"Bae","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Aika","family":"Wakata","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fumihiro","family":"Kato","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Masafumi","family":"Sakata","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Toshiyuki","family":"Yamaji","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhiyu","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Makoto","family":"Takeda","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"340","published-online":{"date-parts":[[2022,12,8]]},"reference":[{"issue":"9616","key":"ppat.1010949.ref001","doi-asserted-by":"crossref","first-page":"932","DOI":"10.1016\/S0140-6736(08)60419-5","article-title":"Mumps.","volume":"371","author":"A Hviid","year":"2008","journal-title":"Lancet"},{"issue":"2","key":"ppat.1010949.ref002","doi-asserted-by":"crossref","first-page":"242","DOI":"10.1002\/path.4445","article-title":"Molecular biology, pathogenesis and pathology of mumps virus","volume":"235","author":"S Rubin","year":"2015","journal-title":"J Pathol"},{"issue":"41","key":"ppat.1010949.ref003","doi-asserted-by":"crossref","first-page":"11579","DOI":"10.1073\/pnas.1608383113","article-title":"Trisaccharide containing \u03b12,3-linked sialic acid is a receptor for mumps virus","volume":"113","author":"M Kubota","year":"2016","journal-title":"Proc Natl Acad Sci U S A"},{"issue":"4","key":"ppat.1010949.ref004","doi-asserted-by":"crossref","first-page":"427","DOI":"10.1016\/j.sbi.2007.08.016","article-title":"Structural basis of viral invasion: lessons from paramyxovirus F","volume":"17","author":"RA Lamb","year":"2007","journal-title":"Curr Opin Struct Biol"},{"key":"ppat.1010949.ref005","first-page":"61","article-title":"Transcription and replication of nonsegmented negative-strand RNA viruses","volume":"283","author":"SP Whelan","year":"2004","journal-title":"Curr Top Microbiol Immunol"},{"issue":"8","key":"ppat.1010949.ref006","doi-asserted-by":"crossref","first-page":"3019","DOI":"10.3390\/v6083019","article-title":"Paramyxovirus glycoprotein incorporation, assembly and budding: a three way dance for infectious particle production.","volume":"6","author":"F El Najjar","year":"2014","journal-title":"Viruses"},{"key":"ppat.1010949.ref007","volume-title":"Paramyxoviridae: The Viruses and Their Replication.","author":"RA Lamb","year":"2006","edition":"5"},{"issue":"10","key":"ppat.1010949.ref008","doi-asserted-by":"crossref","first-page":"753","DOI":"10.1038\/nrm934","article-title":"Furin at the cutting edge: from protein traffic to embryogenesis and disease","volume":"3","author":"G. Thomas","year":"2002","journal-title":"Nat Rev Mol Cell Biol"},{"issue":"1","key":"ppat.1010949.ref009","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1006\/viro.1993.1561","article-title":"Paramyxovirus fusion: a hypothesis for changes","volume":"197","author":"RA Lamb","year":"1993","journal-title":"Virology"},{"issue":"2","key":"ppat.1010949.ref010","doi-asserted-by":"crossref","first-page":"332","DOI":"10.1006\/viro.2001.0899","article-title":"Carbohydrate modifications of the NDV fusion protein heptad repeat domains influence maturation and fusion activity","volume":"283","author":"L McGinnes","year":"2001","journal-title":"Virology"},{"issue":"Pt 3","key":"ppat.1010949.ref011","doi-asserted-by":"crossref","first-page":"705","DOI":"10.1099\/0022-1317-76-3-705","article-title":"Influence of N-linked oligosaccharide chains on the processing, cell surface expression and function of the measles virus fusion protein","volume":"76","author":"A 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Diabate","year":"1984","journal-title":"Eur J Biochem"},{"issue":"11","key":"ppat.1010949.ref033","doi-asserted-by":"crossref","first-page":"5355","DOI":"10.1016\/S0021-9258(19)69207-0","article-title":"Carbohydrate structures of HVJ (Sendai virus) glycoproteins.","volume":"256","author":"H Yoshima","year":"1981","journal-title":"J Biol Chem"},{"issue":"19","key":"ppat.1010949.ref034","doi-asserted-by":"crossref","first-page":"9669","DOI":"10.1016\/S0021-9258(19)83568-8","article-title":"The carbohydrate structure of the glycoproteins of the paramyxovirus SV5 grown in bovine kidney cells","volume":"254","author":"P Prehm","year":"1979","journal-title":"J Biol Chem"},{"issue":"4","key":"ppat.1010949.ref035","doi-asserted-by":"crossref","first-page":"271","DOI":"10.1111\/j.1348-0421.1996.tb03346.x","article-title":"Isolation and characterization of mumps virus strains in a mumps outbreak with a high incidence of aseptic meningitis","volume":"40","author":"H Saito","year":"1996","journal-title":"Microbiol Immunol"},{"issue":"6","key":"ppat.1010949.ref036","doi-asserted-by":"crossref","DOI":"10.1128\/JVI.02220-16","article-title":"Heat Shock Protein 90 Ensures Efficient Mumps Virus Replication by Assisting with Viral Polymerase Complex Formation","volume":"91","author":"H Katoh","year":"2017","journal-title":"J Virol"},{"issue":"6","key":"ppat.1010949.ref037","doi-asserted-by":"crossref","first-page":"3188","DOI":"10.1128\/JVI.03343-14","article-title":"Heat Shock Protein 70 Regulates Degradation of the Mumps Virus Phosphoprotein via the Ubiquitin-Proteasome Pathway","volume":"89","author":"H Katoh","year":"2015","journal-title":"J Virol"},{"issue":"4","key":"ppat.1010949.ref038","doi-asserted-by":"crossref","first-page":"703","DOI":"10.3201\/eid2204.151116","article-title":"Cross-Neutralization between Human and African Bat Mumps Viruses","volume":"22","author":"H Katoh","year":"2016","journal-title":"Emerg Infect Dis"},{"issue":"Pt 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