{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T22:07:19Z","timestamp":1790806039163,"version":"4.1.0"},"update-to":[{"DOI":"10.1371\/journal.ppat.1010994","type":"new_version","label":"New version","source":"publisher","updated":{"date-parts":[[2022,12,22]],"date-time":"2022-12-22T00:00:00Z","timestamp":1671667200000}}],"reference-count":47,"publisher":"Public Library of Science (PLoS)","issue":"12","license":[{"start":{"date-parts":[[2022,12,12]],"date-time":"2022-12-12T00:00:00Z","timestamp":1670803200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"THSTI Intramural grant","award":["THSTI-T001"],"award-info":[{"award-number":["THSTI-T001"]}]},{"DOI":"10.13039\/100000865","name":"Bill and Melinda Gates Foundation","doi-asserted-by":"publisher","award":["Investment INV-030592"],"award-info":[{"award-number":["Investment INV-030592"]}],"id":[{"id":"10.13039\/100000865","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100017220","name":"Regional Centre for Biotechnology","doi-asserted-by":"publisher","award":["RCB- C01001"],"award-info":[{"award-number":["RCB- C01001"]}],"id":[{"id":"10.13039\/100017220","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Ministry of Science and Technology, Government of India"},{"name":"ESRF Access Program of the Department of Biotechnology","award":["BT\/PR36150\/INF\/22\/214\/2020"],"award-info":[{"award-number":["BT\/PR36150\/INF\/22\/214\/2020"]}]}],"content-domain":{"domain":["www.plospathogens.org"],"crossmark-restriction":false},"short-container-title":["PLoS Pathog"],"abstract":"<jats:p>The emergence of new variants of SARS-CoV-2 necessitates unremitting efforts to discover novel therapeutic monoclonal antibodies (mAbs). Here, we report an extremely potent mAb named P4A2 that can neutralize all the circulating variants of concern (VOCs) with high efficiency, including the highly transmissible Omicron. The crystal structure of the P4A2 Fab:RBD complex revealed that the residues of the RBD that interact with P4A2 are a part of the ACE2-receptor-binding motif and are not mutated in any of the VOCs. The pan coronavirus pseudotyped neutralization assay confirmed that the P4A2 mAb is specific for SARS-CoV-2 and its VOCs. Passive administration of P4A2 to K18-hACE2 transgenic mice conferred protection, both prophylactically and therapeutically, against challenge with VOCs. Overall, our data shows that, the P4A2 mAb has immense therapeutic potential to neutralize the current circulating VOCs. Due to the overlap between the P4A2 epitope and ACE2 binding site on spike-RBD, P4A2 may also be highly effective against a number of future variants.<\/jats:p>","DOI":"10.1371\/journal.ppat.1010994","type":"journal-article","created":{"date-parts":[[2022,12,12]],"date-time":"2022-12-12T18:38:25Z","timestamp":1670870305000},"page":"e1010994","update-policy":"https:\/\/doi.org\/10.1371\/journal.ppat.corrections_policy","source":"Crossref","is-referenced-by-count":12,"title":["A broadly neutralizing monoclonal antibody overcomes the mutational landscape of emerging SARS-CoV-2 variants of concern"],"prefix":"10.1371","volume":"18","author":[{"given":"Hilal Ahmad","family":"Parray","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Naveen","family":"Narayanan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sonal","family":"Garg","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zaigham Abbas","family":"Rizvi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tripti","family":"Shrivastava","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sachin","family":"Kushwaha","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Janmejay","family":"Singh","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Praveenkumar","family":"Murugavelu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anbalagan","family":"Anantharaj","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Farha","family":"Mehdi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nisha","family":"Raj","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shivam","family":"Singh","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jyotsna","family":"Dandotiya","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Asha","family":"Lukose","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Deepti","family":"Jamwal","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sandeep","family":"Kumar","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Adarsh K.","family":"Chiranjivi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Samridhi","family":"Dhyani","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nitesh","family":"Mishra","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sanjeev","family":"Kumar","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kamini","family":"Jakhar","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sudipta","family":"Sonar","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anil Kumar","family":"Panchal","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Manas Ranjan","family":"Tripathy","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shirlie Roy","family":"Chowdhury","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shubbir","family":"Ahmed","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sweety","family":"Samal","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shailendra","family":"Mani","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sankar","family":"Bhattacharyya","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Supratik","family":"Das","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Subrata","family":"Sinha","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kalpana","family":"Luthra","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gaurav","family":"Batra","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Devinder","family":"Sehgal","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guruprasad R.","family":"Medigeshi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chandresh","family":"Sharma","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Amit","family":"Awasthi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pramod Kumar","family":"Garg","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Deepak T.","family":"Nair","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rajesh","family":"Kumar","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"340","published-online":{"date-parts":[[2022,12,12]]},"reference":[{"key":"ppat.1010994.ref001","first-page":"1","article-title":"Omicron escapes the majority of existing SARS-CoV-2 neutralizing antibodies","author":"Y Cao","year":"2021","journal-title":"Nature"},{"key":"ppat.1010994.ref002","article-title":"An infectious SARS-CoV-2 B.1.1.529 Omicron virus escapes neutralization by therapeutic monoclonal antibodies","author":"LA VanBlargan","year":"2022","journal-title":"Nat Med"},{"key":"ppat.1010994.ref003","first-page":"581","article-title":"Structure of the SARS-CoV-2 spike receptor-binding domain bound to the ACE2 receptor","year":"2020","journal-title":"Nature"},{"key":"ppat.1010994.ref004","article-title":"Extremely potent monoclonal antibodies neutralize Omicron and other SARS-CoV-2 variants","author":"Z Chen","year":"2022","journal-title":"medRxiv"},{"key":"ppat.1010994.ref005","doi-asserted-by":"crossref","first-page":"eabk3445","DOI":"10.1126\/scitranslmed.abk3445","article-title":"Predicting the mutational drivers of future SARS-CoV-2 variants of concern","volume":"14","author":"MC Maher","year":"2022","journal-title":"Sci Transl Med"},{"key":"ppat.1010994.ref006","doi-asserted-by":"crossref","first-page":"106698","DOI":"10.1016\/j.ijsu.2022.106698","article-title":"The recently emerged BA.4 and BA.5 lineages of Omicron and their global health concerns amid the ongoing wave of COVID-19 pandemic\u2013Correspondence","volume":"103","author":"RK Mohapatra","year":"2022","journal-title":"Int J Surg"},{"key":"ppat.1010994.ref007","doi-asserted-by":"crossref","first-page":"603","DOI":"10.1038\/s41586-022-05053-w","article-title":"Antibody evasion by SARS-CoV-2 Omicron subvariants BA.2.12.1, BA.4 and BA.5","volume":"608","author":"Q Wang","year":"2022","journal-title":"Nature"},{"key":"ppat.1010994.ref008","article-title":"Imprinted antibody responses against SARS-CoV-2 Omicron sublineages","author":"Y-J Park","year":"2022","journal-title":"bioRxiv"},{"key":"ppat.1010994.ref009","article-title":"LY-CoV1404 (bebtelovimab) potently neutralizes SARS-CoV-2 variants","author":"K Westendorf","year":"2022","journal-title":"bioRxiv"},{"key":"ppat.1010994.ref010","doi-asserted-by":"crossref","first-page":"691","DOI":"10.1038\/s41422-022-00677-z","article-title":"Selection and structural bases of potent broadly neutralizing antibodies from 3-dose vaccinees that are highly effective against diverse SARS-CoV-2 variants, including Omicron sublineages","volume":"32","author":"L Wang","year":"2022","journal-title":"Cell Res"},{"key":"ppat.1010994.ref011","article-title":"Antibody-mediated broad sarbecovirus neutralization through ACE2 molecular mimicry","author":"Y-J Park","year":"2021","journal-title":"bioRxiv"},{"key":"ppat.1010994.ref012","doi-asserted-by":"crossref","first-page":"110770","DOI":"10.1016\/j.celrep.2022.110770","article-title":"Structures of Omicron spike complexes and implications for neutralizing antibody development","volume":"39","author":"H Guo","year":"2022","journal-title":"Cell Reports"},{"key":"ppat.1010994.ref013","doi-asserted-by":"crossref","first-page":"eabp9312","DOI":"10.1126\/sciimmunol.abp9312","article-title":"An ACE2-blocking antibody confers broad neutralization and protection against Omicron and other SARS-CoV-2 variants of concern","volume":"7","author":"W Du","year":"2022","journal-title":"Sci Immunol"},{"key":"ppat.1010994.ref014","doi-asserted-by":"crossref","first-page":"546","DOI":"10.1038\/s41586-022-04581-9","article-title":"Molecular basis of receptor binding and antibody neutralization of Omicron","volume":"604","author":"Q Hong","year":"2022","journal-title":"Nature"},{"key":"ppat.1010994.ref015","doi-asserted-by":"crossref","first-page":"491","DOI":"10.1038\/s41422-022-00638-6","article-title":"Immune escape by SARS-CoV-2 Omicron variant and structural basis of its effective neutralization by a broad neutralizing human antibody VacW-209","volume":"32","author":"B Ju","year":"2022","journal-title":"Cell Res"},{"key":"ppat.1010994.ref016","doi-asserted-by":"crossref","first-page":"950","DOI":"10.1126\/science.abe3354","article-title":"Ultrapotent human antibodies protect against SARS-CoV-2 challenge via multiple mechanisms","volume":"370","author":"MA Tortorici","year":"2020","journal-title":"Science"},{"key":"ppat.1010994.ref017","unstructured":"Structural basis for potent antibody neutralization of SARS-CoV-2 variants including B.1.1.529\u2014PubMed. [cited 23 Oct 2022]. Available: https:\/\/pubmed.ncbi.nlm.nih.gov\/35324257\/"},{"key":"ppat.1010994.ref018","unstructured":"Germinal center-derived broadly neutralizing antibodies adapt to SARS-CoV-2 antigenic drift | bioRxiv. [cited 23 Oct 2022]. Available: https:\/\/www.biorxiv.org\/content\/10.1101\/2022.01.26.477937v1.full"},{"key":"ppat.1010994.ref019","article-title":"A potent SARS-CoV-2 neutralizing antibody recognizing a conserved epitope with broad mutant variant and SARS-CoV activity","author":"AJ Pelzek","year":"2022","journal-title":"bioRxiv"},{"key":"ppat.1010994.ref020","article-title":"SARS-CoV-2 hijacks neutralizing dimeric IgA for enhanced nasal infection and injury","author":"B Zhou","year":"2021","journal-title":"bioRxiv"},{"key":"ppat.1010994.ref021","first-page":"2022","article-title":"Structural basis of Omicron neutralization by affinity-matured public antibodies","author":"DJ Sheward","year":"2022","journal-title":"bioRxiv"},{"key":"ppat.1010994.ref022","unstructured":"Kinetics of viral load, immunological mediators and characterization of a SARS-CoV-2 isolate in mild COVID-19 patients during acute phase of infection | medRxiv. [cited 26 Feb 2022]. Available: https:\/\/www.medrxiv.org\/content\/10.1101\/2020.11.05.20226621v2"},{"key":"ppat.1010994.ref023","first-page":"0","article-title":"Effectiveness of ChAdOx1 nCoV-19 vaccine against SARS-CoV-2 infection during the delta (B.1.617.2) variant surge in India: a test-negative, case-control study and a mechanistic study of post-vaccination immune responses","author":"R Thiruvengadam","year":"2021","journal-title":"The Lancet Infectious Diseases"},{"key":"ppat.1010994.ref024","doi-asserted-by":"crossref","first-page":"460","DOI":"10.3201\/eid2802.212422","article-title":"Probable Transmission of SARS-CoV-2 Omicron Variant in Quarantine Hotel, Hong Kong, China, November 2021","volume":"28","author":"H Gu","year":"2022","journal-title":"Emerg Infect Dis"},{"key":"ppat.1010994.ref025","article-title":"Identification of an anti-SARS-CoV-2 receptor binding domain directed human monoclonal antibody from a na\u00efve semi-synthetic library","author":"HA Parray","year":"2020","journal-title":"J Biol Chem"},{"key":"ppat.1010994.ref026","article-title":"Cross-neutralization of SARS-CoV-2 by HIV-1 specific broadly neutralizing antibodies and polyclonal plasma","author":"N Mishra","year":"2020","journal-title":"Immunology"},{"key":"ppat.1010994.ref027","doi-asserted-by":"crossref","first-page":"87","DOI":"10.1186\/1472-6750-12-87","article-title":"A novel strategy for efficient production of anti-V3 human scFvs against HIV-1 clade C","volume":"12","author":"R Kumar","year":"2012","journal-title":"BMC Biotechnol"},{"key":"ppat.1010994.ref028","doi-asserted-by":"crossref","first-page":"1011","DOI":"10.1007\/s12010-018-2862-8","article-title":"Isolation and Characterization of Cross-Neutralizing Human Anti-V3 Single-Chain Variable Fragments (scFvs) Against HIV-1 from an Antigen Preselected Phage Library","volume":"187","author":"R Kumar","year":"2019","journal-title":"Applied Biochemistry and Biotechnology"},{"key":"ppat.1010994.ref029","doi-asserted-by":"crossref","first-page":"108020","DOI":"10.1016\/j.intimp.2021.108020","article-title":"Non-neutralizing SARS CoV-2 directed polyclonal antibodies demonstrate cross-reactivity with the HA glycans of influenza virus","volume":"99","author":"P Murugavelu","year":"2021","journal-title":"International Immunopharmacology"},{"key":"ppat.1010994.ref030","unstructured":"Sub-optimal Neutralisation of Omicron (B.1.1.529) Variant by Antibodies induced by Vaccine alone or SARS-CoV-2 Infection plus Vaccine (Hybrid Immunity) post 6-months | medRxiv. [cited 20 Feb 2022]. Available: https:\/\/www.medrxiv.org\/content\/10.1101\/2022.01.04.22268747v1"},{"key":"ppat.1010994.ref031","doi-asserted-by":"crossref","first-page":"e3136","DOI":"10.1002\/btpr.3136","article-title":"A rapid novel strategy for screening of antibody phage libraries for production, purification, and functional characterization of amber stop codons containing single-chain antibody fragments","author":"R Perween","year":"2021","journal-title":"Biotechnol Prog"},{"key":"ppat.1010994.ref032","doi-asserted-by":"crossref","first-page":"1757","DOI":"10.1107\/S1399004715011918","article-title":"Fully automatic characterization and data collection from crystals of biological macromolecules","volume":"71","author":"O Svensson","year":"2015","journal-title":"Acta Crystallogr D Biol Crystallogr"},{"key":"ppat.1010994.ref033","doi-asserted-by":"crossref","first-page":"271","DOI":"10.1107\/S0907444910048675","article-title":"iMOSFLM: a new graphical interface for diffraction-image processing with MOSFLM","volume":"67","author":"TGG Battye","year":"2011","journal-title":"Acta Crystallogr D Biol Crystallogr"},{"key":"ppat.1010994.ref034","doi-asserted-by":"crossref","first-page":"1204","DOI":"10.1107\/S0907444913000061","article-title":"How good are my data and what is the resolution?","volume":"69","author":"PR Evans","year":"2013","journal-title":"Acta Crystallogr D Biol Crystallogr"},{"key":"ppat.1010994.ref035","doi-asserted-by":"crossref","first-page":"658","DOI":"10.1107\/S0021889807021206","article-title":"Phaser crystallographic software","volume":"40","author":"AJ McCoy","year":"2007","journal-title":"J Appl Crystallogr"},{"key":"ppat.1010994.ref036","doi-asserted-by":"crossref","first-page":"109433","DOI":"10.1016\/j.celrep.2021.109433","article-title":"A SARS-CoV-2 neutralizing antibody selected from COVID-19 patients binds to the ACE2-RBD interface and is tolerant to most known RBD mutations","volume":"36","author":"F Bertoglio","year":"2021","journal-title":"Cell Rep"},{"key":"ppat.1010994.ref037","doi-asserted-by":"crossref","first-page":"2126","DOI":"10.1107\/S0907444904019158","article-title":"Coot: model-building tools for molecular graphics","volume":"60","author":"P Emsley","year":"2004","journal-title":"Acta Crystallogr D Biol Crystallogr"},{"key":"ppat.1010994.ref038","doi-asserted-by":"crossref","first-page":"861","DOI":"10.1107\/S2059798319011471","article-title":"Macromolecular structure determination using X-rays, neutrons and electrons: recent developments in Phenix","volume":"75","author":"D Liebschner","year":"2019","journal-title":"Acta Crystallogr D Struct Biol"},{"key":"ppat.1010994.ref039","doi-asserted-by":"crossref","first-page":"235","DOI":"10.1107\/S0907444910045749","article-title":"Overview of the CCP4 suite and current developments","volume":"67","author":"MD Winn","year":"2011","journal-title":"Acta Crystallogr D Biol Crystallogr"},{"key":"ppat.1010994.ref040","doi-asserted-by":"crossref","first-page":"864","DOI":"10.1126\/science.abn8652","article-title":"Structural basis of SARS-CoV-2 Omicron immune evasion and receptor engagement","volume":"375","author":"M McCallum","year":"2022","journal-title":"Science"},{"key":"ppat.1010994.ref041","article-title":"Structural diversity of the SARS-CoV-2 Omicron spike","author":"SM-C Gobeil","year":"2022","journal-title":"bioRxiv"},{"key":"ppat.1010994.ref042","doi-asserted-by":"crossref","first-page":"eabg5016","DOI":"10.1126\/sciadv.abg5016","article-title":"High-salt diet mediates interplay between NK cells and gut microbiota to induce potent tumor immunity","volume":"7","author":"ZA Rizvi","year":"2021","journal-title":"Sci Adv"},{"key":"ppat.1010994.ref043","doi-asserted-by":"crossref","first-page":"e73522","DOI":"10.7554\/eLife.73522","article-title":"Golden Syrian hamster as a model to study cardiovascular complications associated with SARS-CoV-2 infection","volume":"11","author":"ZA Rizvi","year":"2022","journal-title":"Elife"},{"key":"ppat.1010994.ref044","doi-asserted-by":"crossref","first-page":"205","DOI":"10.1016\/j.jim.2008.09.017","article-title":"Systematic design and testing of nested (RT-)PCR primers for specific amplification of mouse rearranged\/expressed immunoglobulin variable region genes from small number of B cells","volume":"339","author":"S Rohatgi","year":"2008","journal-title":"J Immunol Methods"},{"key":"ppat.1010994.ref045","doi-asserted-by":"crossref","first-page":"W503","DOI":"10.1093\/nar\/gkn316","article-title":"IMGT\/V-QUEST: the highly customized and integrated system for IG and TR standardized V-J and V-D-J sequence analysis","volume":"36","author":"X Brochet","year":"2008","journal-title":"Nucleic Acids Res"},{"key":"ppat.1010994.ref046","first-page":"695","article-title":"IMGT\/V-QUEST: IMGT standardized analysis of the immunoglobulin (IG) and T cell receptor (TR) nucleotide sequences.","volume":"2011","author":"V Giudicelli","year":"2011","journal-title":"Cold Spring Harb Protoc"},{"key":"ppat.1010994.ref047","doi-asserted-by":"crossref","first-page":"W34","DOI":"10.1093\/nar\/gkt382","article-title":"IgBLAST: an immunoglobulin variable domain sequence analysis tool","volume":"41","author":"J Ye","year":"2013","journal-title":"Nucleic Acids Res"}],"updated-by":[{"DOI":"10.1371\/journal.ppat.1010994","type":"new_version","label":"New version","source":"publisher","updated":{"date-parts":[[2022,12,22]],"date-time":"2022-12-22T00:00:00Z","timestamp":1671667200000}}],"container-title":["PLOS Pathogens"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/dx.plos.org\/10.1371\/journal.ppat.1010994","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,12,2]],"date-time":"2023-12-02T21:26:31Z","timestamp":1701552391000},"score":1,"resource":{"primary":{"URL":"https:\/\/dx.plos.org\/10.1371\/journal.ppat.1010994"}},"subtitle":[],"editor":[{"given":"Sujan","family":"Shresta","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2022,12,12]]},"references-count":47,"journal-issue":{"issue":"12","published-online":{"date-parts":[[2022,12,12]]}},"URL":"https:\/\/doi.org\/10.1371\/journal.ppat.1010994","relation":{"new_version":[{"id-type":"doi","id":"10.1371\/journal.ppat.1010994","asserted-by":"object"}]},"ISSN":["1553-7374"],"issn-type":[{"value":"1553-7374","type":"electronic"}],"subject":[],"published":{"date-parts":[[2022,12,12]]}}}