{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,16]],"date-time":"2026-10-16T08:59:12Z","timestamp":1792141152311,"version":"4.4.0"},"update-to":[{"DOI":"10.1371\/journal.ppat.1011058","type":"new_version","label":"New version","source":"publisher","updated":{"date-parts":[[2023,6,2]],"date-time":"2023-06-02T00:00:00Z","timestamp":1685664000000}}],"reference-count":83,"publisher":"Public Library of Science (PLoS)","issue":"5","license":[{"start":{"date-parts":[[2023,5,22]],"date-time":"2023-05-22T00:00:00Z","timestamp":1684713600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100006492","name":"Division of Intramural Research, National Institute of Allergy and Infectious Diseases","doi-asserted-by":"publisher","award":["AI132356"],"award-info":[{"award-number":["AI132356"]}],"id":[{"id":"10.13039\/100006492","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000057","name":"National Institute of General Medical Sciences","doi-asserted-by":"publisher","award":["GM146795"],"award-info":[{"award-number":["GM146795"]}],"id":[{"id":"10.13039\/100000057","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100006492","name":"Division of Intramural Research, National Institute of Allergy and Infectious Diseases","doi-asserted-by":"publisher","award":["T32AI055396"],"award-info":[{"award-number":["T32AI055396"]}],"id":[{"id":"10.13039\/100006492","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000057","name":"National Institute of General Medical Sciences","doi-asserted-by":"publisher","award":["T32GM007270"],"award-info":[{"award-number":["T32GM007270"]}],"id":[{"id":"10.13039\/100000057","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["www.plospathogens.org"],"crossmark-restriction":false},"short-container-title":["PLoS Pathog"],"abstract":"<jats:p>\n                    <jats:italic>Listeria monocytogenes<\/jats:italic>\n                    (\n                    <jats:italic>Lm<\/jats:italic>\n                    ) is an intracellular foodborne pathogen which causes the severe disease listeriosis in immunocompromised individuals. Macrophages play a dual role during\n                    <jats:italic>Lm<\/jats:italic>\n                    infection by both promoting dissemination of\n                    <jats:italic>Lm<\/jats:italic>\n                    from the gastrointestinal tract and limiting bacterial growth upon immune activation. Despite the relevance of macrophages to\n                    <jats:italic>Lm<\/jats:italic>\n                    infection, the mechanisms underlying phagocytosis of\n                    <jats:italic>Lm<\/jats:italic>\n                    by macrophages are not well understood. To identify host factors important for\n                    <jats:italic>Lm<\/jats:italic>\n                    infection of macrophages, we performed an unbiased CRISPR\/Cas9 screen which revealed pathways that are specific to phagocytosis of\n                    <jats:italic>Lm<\/jats:italic>\n                    and those that are required for internalization of bacteria generally. Specifically, we discovered the tumor suppressor PTEN promotes macrophage phagocytosis of\n                    <jats:italic>Lm<\/jats:italic>\n                    and\n                    <jats:italic>L<\/jats:italic>\n                    .\n                    <jats:italic>ivanovii<\/jats:italic>\n                    , but not other Gram-positive bacteria. Additionally, we found that PTEN enhances phagocytosis of\n                    <jats:italic>Lm<\/jats:italic>\n                    via its lipid phosphatase activity by promoting adherence to macrophages. Using conditional knockout mice lacking\n                    <jats:italic>Pten<\/jats:italic>\n                    in myeloid cells, we show that PTEN-dependent phagocytosis is important for host protection during oral\n                    <jats:italic>Lm<\/jats:italic>\n                    infection. Overall, this study provides a comprehensive identification of macrophage factors involved in regulating\n                    <jats:italic>Lm<\/jats:italic>\n                    uptake and characterizes the function of one factor, PTEN, during\n                    <jats:italic>Lm<\/jats:italic>\n                    infection\n                    <jats:italic>in vitro<\/jats:italic>\n                    and\n                    <jats:italic>in vivo<\/jats:italic>\n                    . Importantly, these results demonstrate a role for opsonin-independent phagocytosis in\n                    <jats:italic>Lm<\/jats:italic>\n                    pathogenesis and suggest that macrophages play a primarily protective role during foodborne listeriosis.\n                  <\/jats:p>","DOI":"10.1371\/journal.ppat.1011058","type":"journal-article","created":{"date-parts":[[2023,5,22]],"date-time":"2023-05-22T13:31:30Z","timestamp":1684762290000},"page":"e1011058","update-policy":"https:\/\/doi.org\/10.1371\/journal.ppat.corrections_policy","source":"Crossref","is-referenced-by-count":8,"title":["A genome-wide screen in macrophages identifies PTEN as required for myeloid restriction of Listeria monocytogenes infection"],"prefix":"10.1371","volume":"19","author":[{"given":"Rochelle C.","family":"Glover","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nicole H.","family":"Schwardt","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shania-Kate E.","family":"Leano","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Madison E.","family":"Sanchez","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maureen K.","family":"Thomason","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andrew J.","family":"Olive","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0770-7784","authenticated-orcid":true,"given":"Michelle L.","family":"Reniere","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"340","published-online":{"date-parts":[[2023,5,22]]},"reference":[{"issue":"11","key":"ppat.1011058.ref001","doi-asserted-by":"crossref","first-page":"5868","DOI":"10.1099\/ijsem.0.004494","article-title":"Listeria valentina sp. nov., isolated from a water trough and the faeces of healthy sheep","volume":"70","author":"JJ Quereda","year":"2020","journal-title":"Int J Syst Evol Microbiol"},{"issue":"3","key":"ppat.1011058.ref002","doi-asserted-by":"crossref","first-page":"584","DOI":"10.1128\/CMR.14.3.584-640.2001","article-title":"Listeria Pathogenesis and Molecular Virulence Determinants","volume":"14","author":"JA V\u00e1zquez-Boland","year":"2001","journal-title":"Clin Microbiol Rev"},{"issue":"11","key":"ppat.1011058.ref003","doi-asserted-by":"crossref","first-page":"e1003015","DOI":"10.1371\/journal.ppat.1003015","article-title":"InlA Promotes Dissemination of Listeria monocytogenes to the Mesenteric Lymph Nodes during Food Borne Infection of Mice","volume":"8","author":"EN Bou Ghanem","year":"2012","journal-title":"PLoS Pathog"},{"issue":"2","key":"ppat.1011058.ref004","doi-asserted-by":"crossref","first-page":"720","DOI":"10.1128\/IAI.05958-11","article-title":"Oral Infection with Signature-Tagged Listeria monocytogenes Reveals Organ-Specific Growth and Dissemination Routes in Guinea Pigs","volume":"80","author":"JA Melton-Witt","year":"2011","journal-title":"Infect Immun"},{"issue":"6","key":"ppat.1011058.ref005","doi-asserted-by":"crossref","first-page":"1407","DOI":"10.1111\/mmi.14836","article-title":"Role of internalin proteins in the pathogenesis of Listeria monocytogenes","volume":"116","author":"K Ireton","year":"2021","journal-title":"Mol Microbiol"},{"issue":"8","key":"ppat.1011058.ref006","doi-asserted-by":"crossref","first-page":"3146","DOI":"10.1128\/IAI.00503-15","article-title":"Intracellular Listeria 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