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They aim to prevent the infection of vectors and thereby reduce the subsequent exposure of a human population to infectious mosquitoes. The effectiveness of these approaches has been shown to depend on the initial intensity of infection in mosquitoes, often measured as the mean number of oocysts resulting from an infectious blood meal in absence of intervention. In mosquitoes exposed to a high intensity of infection, current TBI candidates are expected to be ineffective at completely blocking infection but will decrease parasite load and therefore, potentially also affect key parameters of vector transmission. The present study investigated the consequences of changes in oocyst intensity on subsequent parasite development and mosquito survival. To address this, we experimentally produced different intensities of infection for <jats:italic>Anopheles gambiae<\/jats:italic> females from Burkina Faso by diluting gametocytes from three natural <jats:italic>Plasmodium falciparum<\/jats:italic> local isolates and used a newly developed non-destructive method based on the exploitation of mosquito sugar feeding to track parasite and mosquito life history traits throughout sporogonic development. Our results indicate the extrinsic incubation period (EIP) of <jats:italic>P<\/jats:italic>. <jats:italic>falciparum<\/jats:italic> and mosquito survival did not vary with parasite density but differed significantly between parasite isolates with estimated EIP<jats:sub>50<\/jats:sub> of 16 (95% CI: 15\u201318), 14 (95% CI: 12\u201316) and 12 (95% CI: 12\u201313) days and median longevity of 25 (95% CI: 22\u201329), 15 (95% CI: 13\u201315) and 18 (95% CI: 17\u201319) days for the three isolates respectively. Our results here do not identify unintended consequences of the decrease of parasite loads in mosquitoes on the parasite incubation period or on mosquito survival, two key parameters of vectorial capacity, and hence support the use of transmission blocking strategies to control malaria.<\/jats:p>","DOI":"10.1371\/journal.ppat.1011084","type":"journal-article","created":{"date-parts":[[2023,5,17]],"date-time":"2023-05-17T17:31:04Z","timestamp":1684344664000},"page":"e1011084","update-policy":"https:\/\/doi.org\/10.1371\/journal.ppat.corrections_policy","source":"Crossref","is-referenced-by-count":20,"title":["Intervention reducing malaria parasite load in vector mosquitoes: No impact on Plasmodium falciparum extrinsic incubation period and the survival of Anopheles gambiae"],"prefix":"10.1371","volume":"19","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-9285-027X","authenticated-orcid":true,"given":"Edwige","family":"Guissou","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dari Fr\u00e9d\u00e9ric","family":"Da","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Domombabele Fran\u00e7ois de Sales","family":"Hien","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Koudraogo Bienvenue","family":"Yameogo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Serge Rakiswende","family":"Yerbanga","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Georges Anicet","family":"Ou\u00e9draogo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kounbobr Roch","family":"Dabir\u00e9","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thierry","family":"Lef\u00e8vre","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anna","family":"Cohuet","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"340","published-online":{"date-parts":[[2023,5,17]]},"reference":[{"key":"ppat.1011084.ref001","unstructured":"OMS. 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