{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,23]],"date-time":"2026-10-23T12:49:26Z","timestamp":1792759766629,"version":"4.5.0"},"update-to":[{"DOI":"10.1371\/journal.ppat.1011152","type":"new_version","label":"New version","source":"publisher","updated":{"date-parts":[[2023,5,11]],"date-time":"2023-05-11T00:00:00Z","timestamp":1683763200000}}],"reference-count":51,"publisher":"Public Library of Science (PLoS)","issue":"5","license":[{"start":{"date-parts":[[2023,5,1]],"date-time":"2023-05-01T00:00:00Z","timestamp":1682899200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100000057","name":"National Institute of General Medical Sciences","doi-asserted-by":"publisher","award":["R35GM118027-01"],"award-info":[{"award-number":["R35GM118027-01"]}],"id":[{"id":"10.13039\/100000057","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100006492","name":"Division of Intramural Research, National Institute of Allergy and Infectious Diseases","doi-asserted-by":"publisher","award":["5 R01 AI150669-03"],"award-info":[{"award-number":["5 R01 AI150669-03"]}],"id":[{"id":"10.13039\/100006492","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000049","name":"National Institute on Aging","doi-asserted-by":"publisher","award":["T32AG000213"],"award-info":[{"award-number":["T32AG000213"]}],"id":[{"id":"10.13039\/100000049","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["www.plospathogens.org"],"crossmark-restriction":false},"short-container-title":["PLoS Pathog"],"abstract":"<jats:p>Hyphal growth is essential for host colonization during <jats:italic>Aspergillus<\/jats:italic> infection. The transcription factor ZfpA regulates <jats:italic>A<\/jats:italic>. <jats:italic>fumigatus<\/jats:italic> hyphal development including branching, septation, and cell wall composition. However, how ZfpA affects fungal growth and susceptibility to host immunity during infection has not been investigated. Here, we use the larval zebrafish-<jats:italic>Aspergillus<\/jats:italic> infection model and primary human neutrophils to probe how ZfpA affects <jats:italic>A<\/jats:italic>. <jats:italic>fumigatus<\/jats:italic> pathogenesis and response to antifungal drugs <jats:italic>in vivo<\/jats:italic>. ZfpA deletion promotes fungal clearance and attenuates virulence in wild-type hosts and this virulence defect is abrogated in neutrophil-deficient zebrafish. ZfpA deletion also increases susceptibility to human neutrophils <jats:italic>ex vivo<\/jats:italic> while overexpression impairs fungal killing. Overexpression of ZfpA confers protection against the antifungal caspofungin by increasing chitin synthesis during hyphal development, while ZfpA deletion reduces cell wall chitin and increases caspofungin susceptibility in neutrophil-deficient zebrafish. These findings suggest a protective role for ZfpA activity in resistance to the innate immune response and antifungal treatment during <jats:italic>A<\/jats:italic>. <jats:italic>fumigatus<\/jats:italic> infection.<\/jats:p>","DOI":"10.1371\/journal.ppat.1011152","type":"journal-article","created":{"date-parts":[[2023,5,1]],"date-time":"2023-05-01T17:28:19Z","timestamp":1682962099000},"page":"e1011152","update-policy":"https:\/\/doi.org\/10.1371\/journal.ppat.corrections_policy","source":"Crossref","is-referenced-by-count":30,"title":["Aspergillus fumigatus transcription factor ZfpA regulates hyphal development and alters susceptibility to antifungals and neutrophil killing during infection"],"prefix":"10.1371","volume":"19","author":[{"given":"Taylor J.","family":"Schoen","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dante 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