{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,15]],"date-time":"2026-10-15T03:54:58Z","timestamp":1792036498512,"version":"4.4.0"},"update-to":[{"DOI":"10.1371\/journal.ppat.1011239","type":"new_version","label":"New version","source":"publisher","updated":{"date-parts":[[2023,3,23]],"date-time":"2023-03-23T00:00:00Z","timestamp":1679529600000}}],"reference-count":43,"publisher":"Public Library of Science (PLoS)","issue":"3","license":[{"start":{"date-parts":[[2023,3,13]],"date-time":"2023-03-13T00:00:00Z","timestamp":1678665600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["31930005"],"award-info":[{"award-number":["31930005"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["32000018 and 32170193"],"award-info":[{"award-number":["32000018 and 32170193"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100012165","name":"Key Technologies Research and Development Program","doi-asserted-by":"publisher","award":["2021YFC2300400"],"award-info":[{"award-number":["2021YFC2300400"]}],"id":[{"id":"10.13039\/501100012165","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000057","name":"National Institute of General Medical Sciences","doi-asserted-by":"publisher","award":["R35GM124594"],"award-info":[{"award-number":["R35GM124594"]}],"id":[{"id":"10.13039\/100000057","id-type":"DOI","asserted-by":"publisher"}]},{"name":"The Kamangar family in the form of an endowed chair"}],"content-domain":{"domain":["www.plospathogens.org"],"crossmark-restriction":false},"short-container-title":["PLoS Pathog"],"abstract":"<jats:p>\n                    <jats:italic>Candida auris<\/jats:italic>\n                    is an emerging multidrug-resistant fungal pathogen and a new global threat to human health. A unique morphological feature of this fungus is its multicellular aggregating phenotype, which has been thought to be associated with defects in cell division. In this study, we report a new aggregating form of two clinical\n                    <jats:italic>C<\/jats:italic>\n                    .\n                    <jats:italic>auris<\/jats:italic>\n                    isolates with increased biofilm forming capacity due to enhanced adherence of adjacent cells and surfaces. Unlike the previously reported aggregating morphology, this new aggregating multicellular form of\n                    <jats:italic>C<\/jats:italic>\n                    .\n                    <jats:italic>auris<\/jats:italic>\n                    can become unicellular after treatment with proteinase K or trypsin. Genomic analysis demonstrated that amplification of the subtelomeric adhesin gene\n                    <jats:italic>ALS4<\/jats:italic>\n                    is the reason behind the strain\u2019s enhanced adherence and biofilm forming capacities. Many clinical isolates of\n                    <jats:italic>C<\/jats:italic>\n                    .\n                    <jats:italic>auris<\/jats:italic>\n                    have variable copy numbers of\n                    <jats:italic>ALS4<\/jats:italic>\n                    , suggesting that this subtelomeric region exhibits instability. Global transcriptional profiling and quantitative real-time PCR assays indicated that genomic amplification of\n                    <jats:italic>ALS4<\/jats:italic>\n                    results in a dramatic increase in overall levels of transcription. Compared to the previously characterized nonaggregative\/yeast-form and aggregative-form strains of\n                    <jats:italic>C<\/jats:italic>\n                    .\n                    <jats:italic>auris<\/jats:italic>\n                    , this new Als4-mediated aggregative-form strain of\n                    <jats:italic>C<\/jats:italic>\n                    .\n                    <jats:italic>auris<\/jats:italic>\n                    displays several unique characteristics in terms of its biofilm formation, surface colonization, and virulence.\n                  <\/jats:p>","DOI":"10.1371\/journal.ppat.1011239","type":"journal-article","created":{"date-parts":[[2023,3,13]],"date-time":"2023-03-13T13:39:24Z","timestamp":1678714764000},"page":"e1011239","update-policy":"https:\/\/doi.org\/10.1371\/journal.ppat.corrections_policy","source":"Crossref","is-referenced-by-count":93,"title":["Clinical isolates of Candida auris with enhanced adherence and biofilm formation due to genomic amplification of ALS4"],"prefix":"10.1371","volume":"19","author":[{"given":"Jian","family":"Bing","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhangyue","family":"Guan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tianhong","family":"Zheng","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhijie","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shuru","family":"Fan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Craig L.","family":"Ennis","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Clarissa J.","family":"Nobile","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4761-7548","authenticated-orcid":true,"given":"Guanghua","family":"Huang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"340","published-online":{"date-parts":[[2023,3,13]]},"reference":[{"issue":"10","key":"ppat.1011239.ref001","doi-asserted-by":"crossref","first-page":"e1008921","DOI":"10.1371\/journal.ppat.1008921","article-title":"Candida auris: Epidemiology, biology, antifungal resistance, and virulence.","volume":"16","author":"H Du","year":"2020","journal-title":"PLoS Pathog."},{"issue":"8\u20139","key":"ppat.1011239.ref002","doi-asserted-by":"crossref","first-page":"607","DOI":"10.1016\/j.crvi.2011.05.008","article-title":"The genetic code of the fungal CTG clade","volume":"334","author":"MA Santos","year":"2011","journal-title":"Comptes rendus 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