{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,18]],"date-time":"2026-04-18T20:40:38Z","timestamp":1776544838079,"version":"3.51.2"},"reference-count":45,"publisher":"Public Library of Science (PLoS)","issue":"3","license":[{"start":{"date-parts":[[2023,3,17]],"date-time":"2023-03-17T00:00:00Z","timestamp":1679011200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100007219","name":"Natural Science Foundation of Shanghai","doi-asserted-by":"publisher","award":["19ZR1462600"],"award-info":[{"award-number":["19ZR1462600"]}],"id":[{"id":"10.13039\/100007219","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81971969"],"award-info":[{"award-number":["81971969"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["82272369"],"award-info":[{"award-number":["82272369"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81772225"],"award-info":[{"award-number":["81772225"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["www.plospathogens.org"],"crossmark-restriction":false},"short-container-title":["PLoS Pathog"],"abstract":"<jats:p>\n                    Schistosomiasis is a zoonotic parasitic disease.\n                    <jats:italic>Schistosoma japonicum<\/jats:italic>\n                    eggs deposited in the liver tissue induce egg granuloma formation and liver fibrosis, seriously threatening human health. Natural killer (NK) cells kill activated hepatic stellate cells (HSCs) or induce HSC apoptosis and inhibit the progression of liver fibrosis. However, the function of NK cells in liver fibrosis caused by\n                    <jats:italic>S<\/jats:italic>\n                    .\n                    <jats:italic>japonicum<\/jats:italic>\n                    infection is significantly inhibited. The mechanism of this inhibition remains unclear. Twenty mice were percutaneously infected with\n                    <jats:italic>S<\/jats:italic>\n                    .\n                    <jats:italic>japonicum<\/jats:italic>\n                    cercariae. Before infection and 2, 4, 6, and 8 weeks after infection, five mice were euthanized and dissected at each time point. Hepatic NK cells were isolated and transcriptome sequenced. The sequencing results showed that\n                    <jats:italic>Tigit<\/jats:italic>\n                    expression was high at 4\u20136 weeks post infection. This phenomenon was verified by reverse transcription quantitative PCR (RT-qPCR) and flow cytometry. NK cells derived from\n                    <jats:italic>Tigit<\/jats:italic>\n                    <jats:sup>-\/-<\/jats:sup>\n                    and wild-type (WT) mice were co-cultured with HSCs. It was found that\n                    <jats:italic>Tigit<\/jats:italic>\n                    <jats:sup>-\/-<\/jats:sup>\n                    NK cells induced apoptosis in a higher proportion of HSCs than WT NK cells. Schistosomiasis infection models of\n                    <jats:italic>Tigit<\/jats:italic>\n                    <jats:sup>-\/-<\/jats:sup>\n                    and WT mice were established. The proportion and killing activity of hepatic NK cells were significantly higher in\n                    <jats:italic>Tigit<\/jats:italic>\n                    <jats:sup>-\/-<\/jats:sup>\n                    mice than in WT mice. The degree of liver fibrosis in\n                    <jats:italic>Tigit<\/jats:italic>\n                    <jats:sup>-\/-<\/jats:sup>\n                    mice was significantly lower than that in WT mice. NK cells were isolated from\n                    <jats:italic>Tigit<\/jats:italic>\n                    <jats:sup>-\/-<\/jats:sup>\n                    and WT mice and injected via the tail vein into WT mice infected with\n                    <jats:italic>S<\/jats:italic>\n                    .\n                    <jats:italic>japonicum<\/jats:italic>\n                    . The degree of liver fibrosis in mice that received NK cell infusion reduced significantly, but there was no significant difference between mice that received NK cells from\n                    <jats:italic>Tigit<\/jats:italic>\n                    <jats:sup>-\/-<\/jats:sup>\n                    and WT mice, respectively. Our findings indicate that\n                    <jats:italic>Tigit<\/jats:italic>\n                    knockout enhanced the function of NK cells and reduced the degree of liver fibrosis in schistosomiasis, thus providing a novel strategy for treating hepatic fibrosis induced by schistosomiasis.\n                  <\/jats:p>","DOI":"10.1371\/journal.ppat.1011242","type":"journal-article","created":{"date-parts":[[2023,3,17]],"date-time":"2023-03-17T13:37:38Z","timestamp":1679060258000},"page":"e1011242","update-policy":"https:\/\/doi.org\/10.1371\/journal.ppat.corrections_policy","source":"Crossref","is-referenced-by-count":9,"title":["Inhibition of hepatic natural killer cell function via the TIGIT receptor in schistosomiasis-induced liver fibrosis"],"prefix":"10.1371","volume":"19","author":[{"given":"Yuan","family":"Gao","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiaocheng","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tingting","family":"Jiang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hao","family":"Zhou","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hua","family":"Liu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yuan","family":"Hu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1974-0047","authenticated-orcid":true,"given":"Jianping","family":"Cao","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"340","published-online":{"date-parts":[[2023,3,17]]},"reference":[{"issue":"7","key":"ppat.1011242.ref001","doi-asserted-by":"crossref","first-page":"582","DOI":"10.1016\/j.pt.2020.04.012","article-title":"Schistosomiasis 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