{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,22]],"date-time":"2026-10-22T02:54:30Z","timestamp":1792637670741,"version":"4.5.0"},"update-to":[{"DOI":"10.1371\/journal.ppat.1011367","type":"new_version","label":"New version","source":"publisher","updated":{"date-parts":[[2023,5,17]],"date-time":"2023-05-17T00:00:00Z","timestamp":1684281600000}}],"reference-count":62,"publisher":"Public Library of Science (PLoS)","issue":"5","license":[{"start":{"date-parts":[[2023,5,5]],"date-time":"2023-05-05T00:00:00Z","timestamp":1683244800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100009340","name":"Children's Discovery Institute","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100009340","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000888","name":"W. M. 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Classical isolates are considered urgent threats due to their antibiotic resistance profiles, while hv\n                    <jats:italic>Kp<\/jats:italic>\n                    isolates have historically been antibiotic susceptible. Recently, however, increased rates of antibiotic resistance have been observed in both hv\n                    <jats:italic>Kp<\/jats:italic>\n                    and c\n                    <jats:italic>Kp<\/jats:italic>\n                    , further underscoring the need for preventive and effective immunotherapies. Two distinct surface polysaccharides have gained traction as vaccine candidates against\n                    <jats:italic>K<\/jats:italic>\n                    .\n                    <jats:italic>pneumoniae<\/jats:italic>\n                    : capsular polysaccharide and the O-antigen of lipopolysaccharide. While both targets have practical advantages and disadvantages, it remains unclear which of these antigens included in a vaccine would provide superior protection against matched\n                    <jats:italic>K<\/jats:italic>\n                    .\n                    <jats:italic>pneumoniae<\/jats:italic>\n                    strains. Here, we report the production of two bioconjugate vaccines, one targeting the K2 capsular serotype and the other targeting the O1 O-antigen. Using murine models, we investigated whether these vaccines induced specific antibody responses that recognize K2:O1\n                    <jats:italic>K<\/jats:italic>\n                    .\n                    <jats:italic>pneumoniae<\/jats:italic>\n                    strains. While each vaccine was immunogenic in mice, both c\n                    <jats:italic>Kp<\/jats:italic>\n                    and hv\n                    <jats:italic>Kp<\/jats:italic>\n                    strains exhibited decreased O-antibody binding in the presence of capsule. Further, O1 antibodies demonstrated decreased killing in serum bactericidal assays with encapsulated strains, suggesting that the presence of\n                    <jats:italic>K<\/jats:italic>\n                    .\n                    <jats:italic>pneumoniae<\/jats:italic>\n                    capsule blocks O1-antibody binding and function. Finally, the K2 vaccine outperformed the O1 vaccine against both c\n                    <jats:italic>Kp<\/jats:italic>\n                    and hv\n                    <jats:italic>Kp<\/jats:italic>\n                    in two different murine infection models. These data suggest that capsule-based vaccines may be superior to O-antigen vaccines for targeting hv\n                    <jats:italic>Kp<\/jats:italic>\n                    and some c\n                    <jats:italic>Kp<\/jats:italic>\n                    strains, due to capsule blocking the O-antigen.\n                  <\/jats:p>","DOI":"10.1371\/journal.ppat.1011367","type":"journal-article","created":{"date-parts":[[2023,5,5]],"date-time":"2023-05-05T13:38:47Z","timestamp":1683293927000},"page":"e1011367","update-policy":"https:\/\/doi.org\/10.1371\/journal.ppat.corrections_policy","source":"Crossref","is-referenced-by-count":66,"title":["Capsular polysaccharide inhibits vaccine-induced O-antigen antibody binding and function across both classical and hypervirulent K2:O1 strains of Klebsiella pneumoniae"],"prefix":"10.1371","volume":"19","author":[{"given":"Paeton L.","family":"Wantuch","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cory J.","family":"Knoot","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lloyd S.","family":"Robinson","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Evgeny","family":"Vinogradov","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nichollas E.","family":"Scott","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2158-3546","authenticated-orcid":true,"given":"Christian M.","family":"Harding","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1081-772X","authenticated-orcid":true,"given":"David A.","family":"Rosen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"340","published-online":{"date-parts":[[2023,5,5]]},"reference":[{"issue":"7","key":"ppat.1011367.ref001","doi-asserted-by":"crossref","first-page":"e909","DOI":"10.1016\/S2214-109X(20)30205-9","article-title":"Initial findings from a novel population-based child mortality surveillance approach: a descriptive study","volume":"8","author":"AW Taylor","year":"2020","journal-title":"Lancet Glob Health"},{"issue":"6","key":"ppat.1011367.ref002","doi-asserted-by":"crossref","first-page":"669","DOI":"10.2217\/fmb.12.43","article-title":"Hypervirulent Klebsiella pneumoniae: the next superbug?","volume":"7","author":"AS Shon","year":"2012","journal-title":"Future Microbiol."},{"issue":"18","key":"ppat.1011367.ref003","doi-asserted-by":"crossref","first-page":"1732","DOI":"10.1056\/NEJMoa1801550","article-title":"Changes in prevalence of health care-associated infections 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