{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,12]],"date-time":"2026-04-12T16:27:08Z","timestamp":1776011228067,"version":"3.50.1"},"update-to":[{"DOI":"10.1371\/journal.ppat.1011425","type":"new_version","label":"New version","source":"publisher","updated":{"date-parts":[[2023,6,21]],"date-time":"2023-06-21T00:00:00Z","timestamp":1687305600000}}],"reference-count":57,"publisher":"Public Library of Science (PLoS)","issue":"6","license":[{"start":{"date-parts":[[2023,6,8]],"date-time":"2023-06-08T00:00:00Z","timestamp":1686182400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"crossref","award":["R21AI154026"],"award-info":[{"award-number":["R21AI154026"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/100000001","name":"National Science Foundation","doi-asserted-by":"publisher","award":["EEC 1941543"],"award-info":[{"award-number":["EEC 1941543"]}],"id":[{"id":"10.13039\/100000001","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100020392","name":"Good Ventures Foundation","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100020392","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["www.plospathogens.org"],"crossmark-restriction":false},"short-container-title":["PLoS Pathog"],"abstract":"<jats:p><jats:italic>Cryptosporidium hominis<\/jats:italic> is a serious cause of childhood diarrhea in developing countries. The development of therapeutics is impeded by major technical roadblocks including lack of cryopreservation and simple culturing methods. This impacts the availability of optimized\/standardized singular sources of infectious parasite oocysts for research and human challenge studies. The human <jats:italic>C<\/jats:italic>. <jats:italic>hominis<\/jats:italic> TU502 isolate is currently propagated in gnotobiotic piglets in only one laboratory, which limits access to oocysts. Streamlined cryopreservation could enable creation of a biobank to serve as an oocyst source for research and distribution to other investigators requiring <jats:italic>C<\/jats:italic>. <jats:italic>hominis<\/jats:italic>. Here, we report cryopreservation of <jats:italic>C<\/jats:italic>. <jats:italic>hominis<\/jats:italic> TU502 oocysts by vitrification using specially designed specimen containers scaled to 100 \u03bcL volume. Thawed oocysts exhibit ~70% viability with robust excystation and 100% infection rate in gnotobiotic piglets. The availability of optimized\/standardized sources of oocysts may streamline drug and vaccine evaluation by enabling wider access to biological specimens.<\/jats:p>","DOI":"10.1371\/journal.ppat.1011425","type":"journal-article","created":{"date-parts":[[2023,6,8]],"date-time":"2023-06-08T17:48:29Z","timestamp":1686246509000},"page":"e1011425","update-policy":"https:\/\/doi.org\/10.1371\/journal.ppat.corrections_policy","source":"Crossref","is-referenced-by-count":7,"title":["Scalable cryopreservation of infectious Cryptosporidium hominis oocysts by vitrification"],"prefix":"10.1371","volume":"19","author":[{"given":"Justyna J.","family":"Jaskiewicz","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Denise Ann 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