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Here, we find that LGP2 inhibits Zika virus (ZIKV) and tick-borne encephalitis virus (TBEV) replication independent of IFN induction. Co-immunoprecipitation (Co-IP) and confocal immunofluorescence data suggest that LGP2 likely colocalizes with the replication complex (RC) of ZIKV by interacting with viral RNA-dependent RNA polymerase (RdRP) NS5. We further verify that the regulatory domain (RD) of LGP2 directly interacts with RdRP of NS5 by biolayer interferometry assay. Data from <jats:italic>in vitro<\/jats:italic> RdRP assays indicate that LGP2 may inhibit polymerase activities of NS5 at pre-elongation but not elongation stages, while an RNA-binding-defective LGP2 mutant can still inhibit RdRP activities and virus replication. Taken together, our work suggests that LGP2 can inhibit flavivirus replication through direct interaction with NS5 protein and downregulates its polymerase pre-elongation activities, demonstrating a distinct role of LGP2 beyond its function in innate immune responses.<\/jats:p>","DOI":"10.1371\/journal.ppat.1011620","type":"journal-article","created":{"date-parts":[[2023,9,1]],"date-time":"2023-09-01T17:40:28Z","timestamp":1693590028000},"page":"e1011620","update-policy":"https:\/\/doi.org\/10.1371\/journal.ppat.corrections_policy","source":"Crossref","is-referenced-by-count":8,"title":["LGP2 directly interacts with flavivirus NS5 RNA-dependent RNA polymerase and downregulates its pre-elongation activities"],"prefix":"10.1371","volume":"19","author":[{"given":"Zhongyuan","family":"Tan","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiqin","family":"Wu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Li","family":"Huang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ting","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhenhua","family":"Zheng","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jianhui","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xianliang","family":"Ke","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuan","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yan","family":"Liu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hanzhong","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jianping","family":"Tao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8264-7523","authenticated-orcid":true,"given":"Peng","family":"Gong","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"340","published-online":{"date-parts":[[2023,9,1]]},"reference":[{"key":"ppat.1011620.ref001","doi-asserted-by":"crossref","first-page":"649","DOI":"10.1146\/annurev.mi.44.100190.003245","article-title":"Flavivirus 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Rothenfusser","year":"2005","journal-title":"The Journal of Immunology"},{"issue":"5","key":"ppat.1011620.ref026","doi-asserted-by":"crossref","first-page":"1478","DOI":"10.1002\/hep.29050","article-title":"Laboratory of genetics and physiology 2 (LGP2) plays an essential role in hepatitis C virus infection-induced interferon responses.","volume":"65","author":"L Hei","year":"2017","journal-title":"Hepatology"},{"issue":"2","key":"ppat.1011620.ref027","doi-asserted-by":"crossref","first-page":"214","DOI":"10.1093\/infdis\/jiu076","article-title":"Protective role of LGP2 in influenza virus pathogenesis","volume":"210","author":"M Si-Tahar","year":"2014","journal-title":"The Journal of Infectious Diseases"},{"issue":"4","key":"ppat.1011620.ref028","doi-asserted-by":"crossref","first-page":"1512","DOI":"10.1073\/pnas.0912986107","article-title":"LGP2 is a positive regulator of RIG-I\u2013and MDA5-mediated antiviral responses","volume":"107","author":"T 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conformation-based intra-molecular initiation factor identified in the flavivirus RNA-dependent RNA polymerase","volume":"16","author":"J Wu","year":"2020","journal-title":"Plos Pathogens"},{"issue":"3","key":"ppat.1011620.ref047","doi-asserted-by":"crossref","first-page":"1567","DOI":"10.1093\/nar\/gkaa1250","article-title":"Crystal structure of a tick-borne flavivirus RNA-dependent RNA polymerase suggests a host adaptation hotspot in RNA viruses","volume":"49","author":"J Yang","year":"2021","journal-title":"Nucleic Acids Research"},{"issue":"1","key":"ppat.1011620.ref048","doi-asserted-by":"crossref","first-page":"e2211425120","DOI":"10.1073\/pnas.2211425120","article-title":"Structural basis of transition from initiation to elongation in de novo viral RNA-dependent RNA polymerases","volume":"120","author":"J Wu","year":"2023","journal-title":"Proceedings of the National Academy of Sciences of the United States of 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