{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T20:59:11Z","timestamp":1790801951013,"version":"4.1.0"},"update-to":[{"DOI":"10.1371\/journal.ppat.1011707","type":"new_version","label":"New version","source":"publisher","updated":{"date-parts":[[2023,10,12]],"date-time":"2023-10-12T00:00:00Z","timestamp":1697068800000}}],"reference-count":77,"publisher":"Public Library of Science (PLoS)","issue":"10","license":[{"start":{"date-parts":[[2023,10,2]],"date-time":"2023-10-02T00:00:00Z","timestamp":1696204800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"NIH","award":["AI123360"],"award-info":[{"award-number":["AI123360"]}]},{"name":"NIH","award":["GM089778"],"award-info":[{"award-number":["GM089778"]}]},{"name":"Ruth L. Kirschstein National Research Service Award","award":["GM007185"],"award-info":[{"award-number":["GM007185"]}]},{"name":"UCLA Molecular Biology Institute","award":["Whitcome Fellowship"],"award-info":[{"award-number":["Whitcome Fellowship"]}]},{"name":"Ruth L. Kirschstein National Research Service Award","award":["AI007323"],"award-info":[{"award-number":["AI007323"]}]}],"content-domain":{"domain":["www.plospathogens.org"],"crossmark-restriction":false},"short-container-title":["PLoS Pathog"],"abstract":"<jats:p>The inner membrane complex (IMC) of <jats:italic>Toxoplasma gondii<\/jats:italic> is essential for all phases of the parasite\u2019s life cycle. One of its most critical roles is to act as a scaffold for the assembly of daughter buds during replication by endodyogeny. While many daughter IMC proteins have been identified, most are recruited after bud initiation and are not essential for parasite fitness. Here, we report the identification of IMC43, a novel daughter IMC protein that is recruited at the earliest stages of daughter bud initiation. Using an auxin-inducible degron system we show that depletion of IMC43 results in aberrant morphology, dysregulation of endodyogeny, and an extreme defect in replication. Deletion analyses reveal a region of IMC43 that plays a role in localization and a C-terminal domain that is essential for the protein\u2019s function. TurboID proximity labelling and a yeast two-hybrid screen using IMC43 as bait identify 30 candidate IMC43 binding partners. We investigate two of these: the essential daughter protein IMC32 and a novel daughter IMC protein we named IMC44. We show that IMC43 is responsible for regulating the localization of both IMC32 and IMC44 at specific stages of endodyogeny and that this regulation is dependent on the essential C-terminal domain of IMC43. Using pairwise yeast two-hybrid assays, we determine that this region is also sufficient for binding to both IMC32 and IMC44. As IMC43 and IMC32 are both essential proteins, this work reveals the existence of a bud assembly complex that forms the foundation of the daughter IMC during endodyogeny.<\/jats:p>","DOI":"10.1371\/journal.ppat.1011707","type":"journal-article","created":{"date-parts":[[2023,10,2]],"date-time":"2023-10-02T17:30:00Z","timestamp":1696267800000},"page":"e1011707","update-policy":"https:\/\/doi.org\/10.1371\/journal.ppat.corrections_policy","source":"Crossref","is-referenced-by-count":15,"title":["Identification of IMC43, a novel IMC protein that collaborates with IMC32 to form an essential daughter bud assembly complex in Toxoplasma gondii"],"prefix":"10.1371","volume":"19","author":[{"given":"Rebecca R.","family":"Pasquarelli","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Peter S.","family":"Back","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jihui","family":"Sha","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"James A.","family":"Wohlschlegel","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8481-0638","authenticated-orcid":true,"given":"Peter J.","family":"Bradley","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"340","published-online":{"date-parts":[[2023,10,2]]},"reference":[{"issue":"1","key":"ppat.1011707.ref001","doi-asserted-by":"crossref","first-page":"41","DOI":"10.1079\/AHR2005100","article-title":"Biology and epidemiology of Toxoplasma gondii in man and animals","volume":"6","author":"DE Hill","year":"2005","journal-title":"Anim Health Res Rev"},{"issue":"12","key":"ppat.1011707.ref002","doi-asserted-by":"crossref","first-page":"597","DOI":"10.1016\/j.pt.2004.09.006","article-title":"Clinical features and pathogenesis of severe malaria","volume":"20","author":"CL Mackintosh","year":"2004","journal-title":"Trends Parasitol"},{"issue":"5","key":"ppat.1011707.ref003","doi-asserted-by":"crossref","first-page":"e0004729","DOI":"10.1371\/journal.pntd.0004729","article-title":"The Burden of Cryptosporidium Diarrheal Disease among Children < 24 Months of Age in Moderate\/High Mortality Regions of Sub-Saharan Africa and South Asia, Utilizing Data from the Global Enteric Multicenter Study (GEMS)","volume":"10","author":"SO Sow","year":"2016","journal-title":"PLoS Negl Trop Dis"},{"issue":"8","key":"ppat.1011707.ref004","doi-asserted-by":"crossref","first-page":"590","DOI":"10.1111\/j.1365-3024.2010.01209.x","article-title":"Chasing the golden egg: vaccination against poultry coccidiosis","volume":"32","author":"PA Sharman","year":"2010","journal-title":"Parasite Immunol"},{"issue":"4","key":"ppat.1011707.ref005","doi-asserted-by":"crossref","first-page":"291","DOI":"10.1007\/s11250-006-4181-2","article-title":"Estimated direct economic costs associated with tick-borne diseases on cattle in Tanzania","volume":"38","author":"FM Kivaria","year":"2006","journal-title":"Trop Anim Health Prod"},{"issue":"1","key":"ppat.1011707.ref006","doi-asserted-by":"crossref","first-page":"1","DOI":"10.3347\/kjp.2003.41.1.1","article-title":"Review of Neospora caninum and neosporosis in animals","volume":"41","author":"JP Dubey","year":"2003","journal-title":"Korean J Parasitol"},{"issue":"12","key":"ppat.1011707.ref007","doi-asserted-by":"crossref","first-page":"1385","DOI":"10.1016\/j.ijpara.2009.04.003","article-title":"Toxoplasmosis snapshots: global status of Toxoplasma gondii seroprevalence and implications for pregnancy and congenital toxoplasmosis","volume":"39","author":"G Pappas","year":"2009","journal-title":"Int J Parasitol"},{"issue":"12\u201313","key":"ppat.1011707.ref008","doi-asserted-by":"crossref","first-page":"1217","DOI":"10.1016\/S0020-7519(00)00124-7","article-title":"Toxoplasma gondii: from animals to humans","volume":"30","author":"AM Tenter","year":"2000","journal-title":"Int J Parasitol"},{"issue":"2","key":"ppat.1011707.ref009","doi-asserted-by":"crossref","first-page":"211","DOI":"10.1093\/clinids\/15.2.211","article-title":"Toxoplasmic Encephalitis in AIDS","volume":"15","author":"BJ Luft","year":"1992","journal-title":"Clin Infect Dis"},{"issue":"4","key":"ppat.1011707.ref010","doi-asserted-by":"crossref","first-page":"23","DOI":"10.1891\/0730-0832.20.4.23","article-title":"Congenital Toxoplasmosis","volume":"20","author":"S. 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Ludwiczak","year":"2019","journal-title":"Bioinforma Oxf Engl"},{"issue":"5009","key":"ppat.1011707.ref034","doi-asserted-by":"crossref","first-page":"1162","DOI":"10.1126\/science.252.5009.1162","article-title":"Predicting coiled coils from protein sequences","volume":"252","author":"A Lupas","year":"1991","journal-title":"Science"},{"issue":"6","key":"ppat.1011707.ref035","doi-asserted-by":"crossref","first-page":"1423","DOI":"10.1016\/j.cell.2016.08.019","article-title":"A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes","volume":"166","author":"SM Sidik","year":"2016","journal-title":"Cell"},{"issue":"12","key":"ppat.1011707.ref036","doi-asserted-by":"crossref","first-page":"917","DOI":"10.1038\/nmeth.1401","article-title":"An auxin-based degron system for the rapid depletion of proteins in nonplant cells","volume":"6","author":"K Nishimura","year":"2009","journal-title":"Nat 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Biotechnol"},{"issue":"6817","key":"ppat.1011707.ref040","doi-asserted-by":"crossref","first-page":"211","DOI":"10.1038\/35051615","article-title":"The protein-protein interaction map of Helicobacter pylori","volume":"409","author":"JC Rain","year":"2001","journal-title":"Nature"},{"issue":"3","key":"ppat.1011707.ref041","doi-asserted-by":"crossref","first-page":"376","DOI":"10.1101\/gr.2659105","article-title":"Protein interaction mapping: a Drosophila case study","volume":"15","author":"E Formstecher","year":"2005","journal-title":"Genome Res"},{"issue":"5","key":"ppat.1011707.ref042","doi-asserted-by":"crossref","first-page":"752","DOI":"10.1016\/j.chom.2020.09.011","article-title":"A Comprehensive Subcellular Atlas of the Toxoplasma Proteome via hyperLOPIT Provides Spatial Context for Protein Functions","volume":"28","author":"K Barylyuk","year":"2020","journal-title":"Cell Host 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Motifs. 7. The four-helix bundle: what determines a fold?","volume":"9","author":"S Kamtekar","year":"1995","journal-title":"FASEB J Off Publ Fed Am Soc Exp Biol"},{"issue":"2","key":"ppat.1011707.ref047","doi-asserted-by":"crossref","first-page":"130","DOI":"10.1016\/j.tibs.2016.10.007","article-title":"Coiled Coils\u2013A Model System for the 21st Century","volume":"42","author":"AN Lupas","year":"2017","journal-title":"Trends Biochem Sci"},{"issue":"5","key":"ppat.1011707.ref048","doi-asserted-by":"crossref","first-page":"e0205721","DOI":"10.1128\/mBio.02057-21","article-title":"Revisiting the Role of Toxoplasma gondii ERK7 in the Maintenance and Stability of the Apical Complex","volume":"12","author":"N Dos Santos Pacheco","year":"2021","journal-title":"mBio"},{"issue":"10","key":"ppat.1011707.ref049","doi-asserted-by":"crossref","first-page":"1622","DOI":"10.1128\/EC.00114-06","article-title":"Identification of PhIL1, a novel cytoskeletal protein of the Toxoplasma gondii pellicle, through photosensitized labeling with 5-[125I]iodonaphthalene-1-azide","volume":"5","author":"SD Gilk","year":"2006","journal-title":"Eukaryot Cell"},{"issue":"22","key":"ppat.1011707.ref050","doi-asserted-by":"crossref","first-page":"jcs260083","DOI":"10.1242\/jcs.260083","article-title":"IMC10 and LMF1 mediate mitochondrial morphology through mitochondrion-pellicle contact sites in Toxoplasma gondii","volume":"135","author":"RO Oliveira Souza","year":"2022","journal-title":"J Cell Sci"},{"issue":"1","key":"ppat.1011707.ref051","doi-asserted-by":"crossref","first-page":"321","DOI":"10.1186\/1471-2148-11-321","article-title":"Structural and evolutionary divergence of eukaryotic protein kinases in Apicomplexa","volume":"11","author":"E Talevich","year":"2011","journal-title":"BMC Evol Biol"},{"issue":"1","key":"ppat.1011707.ref052","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1111\/mmi.13715","article-title":"The serine\/threonine phosphatases of apicomplexan parasites","volume":"106","author":"C Yang","year":"2017","journal-title":"Mol Microbiol"},{"issue":"9","key":"ppat.1011707.ref053","doi-asserted-by":"crossref","first-page":"e1002948","DOI":"10.1371\/journal.ppat.1002948","article-title":"A unique protein phosphatase with kelch-like domains (PPKL) in Plasmodium modulates ookinete differentiation, motility and invasion","volume":"8","author":"DS Guttery","year":"2012","journal-title":"PLoS Pathog"},{"key":"ppat.1011707.ref054","article-title":"The protein phosphatase PPKL is a key regulator of daughter parasite development in Toxoplasma gondii","author":"C Yang","year":"2023","journal-title":"BioRxiv"},{"issue":"8","key":"ppat.1011707.ref055","doi-asserted-by":"crossref","first-page":"1106","DOI":"10.1111\/cmi.12571","article-title":"The conserved apicomplexan Aurora kinase TgArk3 is involved in endodyogeny, duplication rate and parasite virulence","volume":"18","author":"L Berry","year":"2016","journal-title":"Cell 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selectable marker","volume":"262","author":"K Kim","year":"1993","journal-title":"Science"},{"issue":"24","key":"ppat.1011707.ref059","doi-asserted-by":"crossref","first-page":"14010","DOI":"10.1074\/jbc.271.24.14010","article-title":"Insertional tagging, cloning, and expression of the Toxoplasma gondii hypoxanthine-xanthine-guanine phosphoribosyltransferase gene: Use as a selectable marker for stable transformation","volume":"271","author":"RG Donald","year":"1996","journal-title":"J Biol Chem"},{"issue":"12","key":"ppat.1011707.ref060","doi-asserted-by":"crossref","first-page":"5749","DOI":"10.1073\/pnas.92.12.5749","article-title":"Insertional mutagenesis and marker rescue in a protozoan parasite: cloning of the uracil phosphoribosyltransferase locus from Toxoplasma gondii","volume":"92","author":"RG Donald","year":"1995","journal-title":"Proc Natl Acad Sci U S A"},{"issue":"2","key":"ppat.1011707.ref061","doi-asserted-by":"crossref","first-page":"235","DOI":"10.1016\/0166-6851(96)02598-4","article-title":"A novel epitope tag system to study protein targeting and organelle biogenesis in Trypanosoma brucei","volume":"77","author":"P Bastin","year":"1996","journal-title":"Mol Biochem Parasitol"},{"issue":"12","key":"ppat.1011707.ref062","first-page":"3610","article-title":"Isolation of monoclonal antibodies specific for human c-myc proto-oncogene product","volume":"5","author":"GI Evan","year":"1985","journal-title":"Mol Cell Biol"},{"issue":"6","key":"ppat.1011707.ref063","doi-asserted-by":"crossref","first-page":"568","DOI":"10.1038\/nmeth.3365","article-title":"High-performance probes for light and electron microscopy","volume":"12","author":"S Viswanathan","year":"2015","journal-title":"Nat 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depends on palmitoylation but not myristoylation","volume":"184","author":"C Fung","year":"2012","journal-title":"Mol Biochem Parasitol"},{"issue":"3","key":"ppat.1011707.ref067","doi-asserted-by":"crossref","first-page":"349","DOI":"10.1016\/j.chom.2016.02.006","article-title":"Phosphatidic Acid-Mediated Signaling Regulates Microneme Secretion in Toxoplasma","volume":"19","author":"HE Bullen","year":"2016","journal-title":"Cell Host Microbe"},{"issue":"6","key":"ppat.1011707.ref068","doi-asserted-by":"crossref","first-page":"e100450","DOI":"10.1371\/journal.pone.0100450","article-title":"Efficient Genome Engineering of Toxoplasma gondii Using CRISPR\/Cas9","volume":"9","author":"SM Sidik","year":"2014","journal-title":"PLoS ONE"},{"issue":"4","key":"ppat.1011707.ref069","doi-asserted-by":"crossref","first-page":"530","DOI":"10.1128\/EC.00358-08","article-title":"Tagging of endogenous genes in a Toxoplasma gondii strain lacking Ku80","volume":"8","author":"MH 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Xu","year":"2015","journal-title":"J Proteomics"},{"issue":"1","key":"ppat.1011707.ref073","doi-asserted-by":"crossref","first-page":"21","DOI":"10.1021\/pr015504q","article-title":"DTASelect and Contrast: Tools for Assembling and Comparing Protein Identifications from Shotgun Proteomics","volume":"1","author":"DL Tabb","year":"2002","journal-title":"J Proteome Res"},{"issue":"1","key":"ppat.1011707.ref074","doi-asserted-by":"crossref","first-page":"13.4.1","DOI":"10.1002\/0471250953.bi1304s16","article-title":"Validation of Tandem Mass Spectrometry Database Search Results Using DTASelect","volume":"16","author":"D, L. 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