{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,8]],"date-time":"2026-10-08T05:51:24Z","timestamp":1791438684240,"version":"4.3.3"},"reference-count":77,"publisher":"Public Library of Science (PLoS)","issue":"11","license":[{"start":{"date-parts":[[2023,11,21]],"date-time":"2023-11-21T00:00:00Z","timestamp":1700524800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001407","name":"Department of Biotechnology, Ministry of Science and Technology, India","doi-asserted-by":"publisher","award":["BT\/COE\/34\/SP15138\/2015"],"award-info":[{"award-number":["BT\/COE\/34\/SP15138\/2015"]}],"id":[{"id":"10.13039\/501100001407","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100009053","name":"The Wellcome Trust DBT India Alliance","doi-asserted-by":"publisher","award":["IA\/TSG\/21\/1\/600261"],"award-info":[{"award-number":["IA\/TSG\/21\/1\/600261"]}],"id":[{"id":"10.13039\/501100009053","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["www.plospathogens.org"],"crossmark-restriction":false},"short-container-title":["PLoS Pathog"],"abstract":"<jats:p>\n                    Signalling pathways in malaria parasite remain poorly defined and major reason for this is the lack of understanding of the function of majority of parasite protein kinases and phosphatases in parasite signalling and its biology. In the present study, we have elucidated the function of Protein Kinase 2 (PfPK2), which is known to be indispensable for the survival of human malaria parasite\n                    <jats:italic>Plasmodium falciparum<\/jats:italic>\n                    . We demonstrate that it is involved in the invasion of host erythrocytes, which is critical for establishing infection. In addition, PfPK2 may also be involved in the maturation of the parasite post-invasion. PfPK2 regulates the release of microneme proteins like Apical Membrane Antigen 1 (AMA1), which facilitates the formation of Tight Junction between the merozoite and host erythrocyte- a key step in the process of invasion. Comparative phosphoproteomics studies revealed that PfPK2 may be involved in regulation of several key proteins involved in invasion and signalling. Furthermore, PfPK2 regulates the generation of cGMP and the release of calcium in the parasite, which are key second messengers for the process of invasion. These and other studies have shed light on a novel signalling pathway in which PfPK2 acts as an upstream regulator of important cGMP-calcium signalling, which plays an important role in parasite invasion.\n                  <\/jats:p>","DOI":"10.1371\/journal.ppat.1011770","type":"journal-article","created":{"date-parts":[[2023,11,21]],"date-time":"2023-11-21T13:23:38Z","timestamp":1700573018000},"page":"e1011770","update-policy":"https:\/\/doi.org\/10.1371\/journal.ppat.corrections_policy","source":"Crossref","is-referenced-by-count":8,"title":["Protein kinase PfPK2 mediated signalling is critical for host erythrocyte invasion by malaria parasite"],"prefix":"10.1371","volume":"19","author":[{"given":"Rahul Singh","family":"Rawat","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ankit","family":"Gupta","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Neelam","family":"Antil","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sonika","family":"Bhatnagar","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Monika","family":"Singh","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Akanksha","family":"Rawat","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"T. S. Keshava","family":"Prasad","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6861-0508","authenticated-orcid":true,"given":"Pushkar","family":"Sharma","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"340","published-online":{"date-parts":[[2023,11,21]]},"reference":[{"key":"ppat.1011770.ref001","volume-title":"World Malaria Report","author":"World Health Oganization","year":"2022"},{"key":"ppat.1011770.ref002","doi-asserted-by":"crossref","first-page":"101919","DOI":"10.1016\/j.parint.2019.04.016","article-title":"Efficacy and resistance of different artemisinin-based combination therapies: a systematic review and network meta-analysis","volume":"74","author":"PG Mathenge","year":"2020","journal-title":"Parasitology 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J Biochem"},{"issue":"1","key":"ppat.1011770.ref033","doi-asserted-by":"crossref","first-page":"212","DOI":"10.1093\/nar\/gkg081","article-title":"PlasmoDB: the Plasmodium genome resource. 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Thomas","year":"2012","journal-title":"PLoS One"},{"issue":"3","key":"ppat.1011770.ref052","doi-asserted-by":"crossref","first-page":"e33845","DOI":"10.1371\/journal.pone.0033845","article-title":"Subcellular location, phosphorylation and assembly into the motor complex of GAP45 during Plasmodium falciparum schizont development","volume":"7","author":"MA Ridzuan","year":"2012","journal-title":"PLoS One"},{"issue":"27","key":"ppat.1011770.ref053","doi-asserted-by":"crossref","first-page":"16946","DOI":"10.1074\/jbc.272.27.16946","article-title":"Role of the glycine triad in the ATP-binding site of cAMP-dependent protein kinase","volume":"272","author":"W Hemmer","year":"1997","journal-title":"J Biol Chem"},{"key":"ppat.1011770.ref054","doi-asserted-by":"crossref","first-page":"181","DOI":"10.1016\/j.phrs.2018.07.009","article-title":"Post-translational modifications at the ATP-positioning G-loop that regulate protein kinase activity","volume":"135","author":"SF Steinberg","year":"2018","journal-title":"Pharmacol Res"},{"issue":"6","key":"ppat.1011770.ref055","doi-asserted-by":"crossref","first-page":"e1000941","DOI":"10.1371\/journal.ppat.1000941","article-title":"Protein kinase a dependent phosphorylation of apical membrane antigen 1 plays an important role in erythrocyte invasion by the malaria parasite","volume":"6","author":"K Leykauf","year":"2010","journal-title":"PLoS Pathog"},{"issue":"9","key":"ppat.1011770.ref056","doi-asserted-by":"crossref","first-page":"e1000599","DOI":"10.1371\/journal.ppat.1000599","article-title":"A cyclic GMP signalling module that regulates gliding motility in a malaria parasite","volume":"5","author":"RW Moon","year":"2009","journal-title":"PLoS Pathog"},{"issue":"9","key":"ppat.1011770.ref057","doi-asserted-by":"crossref","first-page":"5589","DOI":"10.1074\/jbc.M708465200","article-title":"Role of Ca2+\/Calmodulin-PfPKB Signaling Pathway in Erythrocyte Invasion by Plasmodium falciparum","volume":"283","author":"A Vaid","year":"2008","journal-title":"J Biol Chem"},{"issue":"13","key":"ppat.1011770.ref058","doi-asserted-by":"crossref","DOI":"10.1126\/sciadv.abe5396","article-title":"Ca(2+) signals critical for egress and gametogenesis in malaria parasites depend on a multipass membrane protein that interacts with PKG","volume":"7","author":"AC Balestra","year":"2021","journal-title":"Science advances"},{"key":"ppat.1011770.ref059","doi-asserted-by":"crossref","first-page":"D447","DOI":"10.1093\/nar\/gku1003","article-title":"STRING v10: protein-protein interaction networks, integrated over the tree of life","volume":"43","author":"D Szklarczyk","year":"2015","journal-title":"Nucleic Acids Res"},{"issue":"30","key":"ppat.1011770.ref060","doi-asserted-by":"crossref","first-page":"27910","DOI":"10.1074\/jbc.M304193200","article-title":"The digestive food vacuole of the malaria parasite is a dynamic intracellular Ca2+ store","volume":"278","author":"GA 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