{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,12]],"date-time":"2026-09-12T02:44:43Z","timestamp":1789181083310,"version":"build-2803163510"},"update-to":[{"DOI":"10.1371\/journal.ppat.1011831","type":"new_version","label":"New version","source":"publisher","updated":{"date-parts":[[2023,12,27]],"date-time":"2023-12-27T00:00:00Z","timestamp":1703635200000}}],"reference-count":62,"publisher":"Public Library of Science (PLoS)","issue":"12","license":[{"start":{"date-parts":[[2023,12,13]],"date-time":"2023-12-13T00:00:00Z","timestamp":1702425600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"the National Key Research and Development Program of China","award":["2021YFC2300800, 2021YFC2300802 and 2021YFC2300804"],"award-info":[{"award-number":["2021YFC2300800, 2021YFC2300802 and 2021YFC2300804"]}]},{"name":"the Shanxi Provincial Agricultural and Rural Research Program","award":["LXXMsxnd202101"],"award-info":[{"award-number":["LXXMsxnd202101"]}]},{"DOI":"10.13039\/501100004775","name":"the Natural Science Foundation of Gansu Province, China","doi-asserted-by":"crossref","award":["23JRRA555"],"award-info":[{"award-number":["23JRRA555"]}],"id":[{"id":"10.13039\/501100004775","id-type":"DOI","asserted-by":"crossref"}]},{"name":"the Research Funding from Lanzhou Veterinary Research Institute","award":["CAASASTIP-JBGS-20210801"],"award-info":[{"award-number":["CAASASTIP-JBGS-20210801"]}]},{"DOI":"10.13039\/501100011136","name":"the State Key Laboratory of Veterinary Etiological Biology","doi-asserted-by":"crossref","award":["SKLVEB2020YQRC01"],"award-info":[{"award-number":["SKLVEB2020YQRC01"]}],"id":[{"id":"10.13039\/501100011136","id-type":"DOI","asserted-by":"crossref"}]},{"name":"the Fundamental Research Funds of the Chinese Academy of Agricultural Sciences","award":["1610032021017"],"award-info":[{"award-number":["1610032021017"]}]}],"content-domain":{"domain":["www.plospathogens.org"],"crossmark-restriction":false},"short-container-title":["PLoS Pathog"],"abstract":"<jats:p>\n                    Protein phosphatases are post-translational regulators of\n                    <jats:italic>Toxoplasma gondii<\/jats:italic>\n                    proliferation, tachyzoite-bradyzoite differentiation and pathogenesis. Here, we identify the putative protein phosphatase 6 (TgPP6) subunits of\n                    <jats:italic>T<\/jats:italic>\n                    .\n                    <jats:italic>gondii<\/jats:italic>\n                    and elucidate their role in the parasite lytic cycle. The putative catalytic subunit TgPP6C and regulatory subunit TgPP6R likely form a complex whereas the predicted structural subunit TgPP6S, with low homology to the human PP6 structural subunit, does not coassemble with TgPP6C and TgPP6R. Functional studies showed that TgPP6C and TgPP6R are essential for parasite growth and replication. The ablation of TgPP6C significantly reduced the synchronous division of the parasite\u2019s daughter cells during endodyogeny, resulting in disordered rosettes. Moreover, the six conserved motifs of TgPP6C were required for efficient endodyogeny. Phosphoproteomic analysis revealed that ablation of TgPP6C predominately altered the phosphorylation status of proteins involved in the regulation of the parasite cell cycle. Deletion of TgPP6C significantly attenuated the parasite virulence in mice. Immunization of mice with TgPP6C-deficient type I RH strain induced protective immunity against challenge with a lethal dose of RH or PYS tachyzoites and Pru cysts. Taken together, the results show that TgPP6C contributes to the cell division, replication and pathogenicity in\n                    <jats:italic>T<\/jats:italic>\n                    .\n                    <jats:italic>gondii<\/jats:italic>\n                    .\n                  <\/jats:p>","DOI":"10.1371\/journal.ppat.1011831","type":"journal-article","created":{"date-parts":[[2023,12,13]],"date-time":"2023-12-13T13:35:26Z","timestamp":1702474526000},"page":"e1011831","update-policy":"https:\/\/doi.org\/10.1371\/journal.ppat.corrections_policy","source":"Crossref","is-referenced-by-count":11,"title":["The Toxoplasma protein phosphatase 6 catalytic subunit (TgPP6C) is essential for cell cycle progression and virulence"],"prefix":"10.1371","volume":"19","author":[{"given":"Qin-Li","family":"Liang","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lan-Bi","family":"Nie","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hany M.","family":"Elsheikha","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ting-Ting","family":"Li","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Li-Xiu","family":"Sun","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhi-Wei","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Meng","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bao-Quan","family":"Fu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2530-4628","authenticated-orcid":true,"given":"Xing-Quan","family":"Zhu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jin-Lei","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"340","published-online":{"date-parts":[[2023,12,13]]},"reference":[{"issue":"9425","key":"ppat.1011831.ref001","doi-asserted-by":"crossref","first-page":"1965","DOI":"10.1016\/S0140-6736(04)16412-X","article-title":"Toxoplasmosis","volume":"363","author":"JG Montoya","year":"2004","journal-title":"Lancet"},{"issue":"1","key":"ppat.1011831.ref002","first-page":"e00115","article-title":"Epidemiology, pathophysiology, diagnosis, and management of cerebral toxoplasmosis","volume":"34","author":"HM Elsheikha","year":"2021","journal-title":"Clin Microbiol Rev"},{"issue":"4","key":"ppat.1011831.ref003","doi-asserted-by":"crossref","first-page":"e0140921","DOI":"10.1128\/mBio.01409-21","article-title":"Organelle dynamics in apicomplexan parasites","volume":"12","author":"JMJ Verhoef","year":"2021","journal-title":"mBio"},{"issue":"3","key":"ppat.1011831.ref004","doi-asserted-by":"crossref","first-page":"e1002093","DOI":"10.1371\/journal.pbio.1002093","article-title":"A novel bipartite centrosome coordinates the apicomplexan cell cycle","volume":"13","author":"ES Suvorova","year":"2015","journal-title":"PLoS 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15","key":"ppat.1011831.ref008","first-page":"3344","article-title":"The Toxoplasma gondii centrosome is the platform for internal daughter budding as revealed by a Nek1 kinase mutant","volume":"126","author":"CT Chen","year":"2013","journal-title":"J Cell Sci"},{"issue":"1","key":"ppat.1011831.ref009","doi-asserted-by":"crossref","first-page":"125","DOI":"10.1016\/j.molbiopara.2005.09.015","article-title":"Golgi and centrosome cycles in Toxoplasma gondii","volume":"145","author":"J Hartmann","year":"2006","journal-title":"Mol Biochem Parasitol"},{"issue":"Pt 9","key":"ppat.1011831.ref010","doi-asserted-by":"crossref","first-page":"1559","DOI":"10.1242\/jcs.021089","article-title":"Organellar dynamics during the cell cycle of Toxoplasma gondii","volume":"121","author":"M Nishi","year":"2008","journal-title":"J Cell Sci"},{"issue":"5","key":"ppat.1011831.ref011","doi-asserted-by":"crossref","first-page":"632","DOI":"10.1111\/cmi.12285","article-title":"The inner membrane complex through 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Shi","year":"2009","journal-title":"Cell"},{"issue":"1","key":"ppat.1011831.ref016","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1111\/mmi.13715","article-title":"The serine\/threonine phosphatases of apicomplexan parasites","volume":"106","author":"C Yang","year":"2017","journal-title":"Mol Microbiol"},{"issue":"1","key":"ppat.1011831.ref017","doi-asserted-by":"crossref","first-page":"7560","DOI":"10.1038\/s41467-022-35267-5","article-title":"The protein phosphatase 2A holoenzyme is a key regulator of starch metabolism and bradyzoite differentiation in Toxoplasma gondii","volume":"13","author":"JL Wang","year":"2022","journal-title":"Nat Commun"},{"issue":"10","key":"ppat.1011831.ref018","doi-asserted-by":"crossref","first-page":"532","DOI":"10.1007\/s00018-022-04556-z","article-title":"The beta subunit of AMP-activated protein kinase is critical for cell cycle progression and parasite development in Toxoplasma gondii","volume":"79","author":"JC Yang","year":"2022","journal-title":"Cell Mol Life SCI"},{"issue":"12","key":"ppat.1011831.ref019","doi-asserted-by":"crossref","first-page":"2631","DOI":"10.1111\/pce.12364","article-title":"Protein phosphatases PP2A, PP4 and PP6: mediators and regulators in development and responses to environmental cues","volume":"37","author":"C Lillo","year":"2014","journal-title":"Plant Cell Environ"},{"key":"ppat.1011831.ref020","doi-asserted-by":"crossref","unstructured":"Cohen PTW. 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Back","year":"2023","journal-title":"mBio"},{"issue":"1","key":"ppat.1011831.ref033","doi-asserted-by":"crossref","first-page":"2813","DOI":"10.1038\/s41467-021-22956-w","article-title":"Toxoplasma LIPIN is essential in channeling host lipid fluxes through membrane biogenesis and lipid storage","volume":"12","author":"S Dass","year":"2021","journal-title":"Nat Commun"},{"key":"ppat.1011831.ref034","doi-asserted-by":"crossref","first-page":"15710","DOI":"10.1038\/ncomms15710","article-title":"Myosin-dependent cell-cell communication controls synchronicity of division in acute and chronic stages of Toxoplasma gondii","volume":"8","author":"K Fr\u00e9nal","year":"2017","journal-title":"Nat Commun"},{"issue":"12","key":"ppat.1011831.ref035","doi-asserted-by":"crossref","first-page":"1069","DOI":"10.1002\/cm.21077","article-title":"Targeted proteomic dissection of Toxoplasma cytoskeleton sub-compartments using MORN1","volume":"69","author":"A Lorestani","year":"2012","journal-title":"Cytoskeleton (Hoboken)"},{"key":"ppat.1011831.ref036","doi-asserted-by":"crossref","first-page":"e50598","DOI":"10.7554\/eLife.50598","article-title":"Differential requirements for cyclase-associated protein (CAP) in actin-dependent processes of Toxoplasma gondii","volume":"8","author":"A Hunt","year":"2019","journal-title":"Elife"},{"issue":"1","key":"ppat.1011831.ref037","doi-asserted-by":"crossref","first-page":"18","DOI":"10.1111\/j.1462-5822.2010.01514.x","article-title":"A family of intermediate filament-like proteins is sequentially assembled into the cytoskeleton of Toxoplasma gondii","volume":"13","author":"BR Anderson-White","year":"2011","journal-title":"Cell Microbiol"},{"issue":"22","key":"ppat.1011831.ref038","doi-asserted-by":"crossref","first-page":"jcs260083","DOI":"10.1242\/jcs.260083","article-title":"IMC10 and LMF1 mediate mitochondrial morphology through mitochondrion-pellicle contact sites in Toxoplasma 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