{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,28]],"date-time":"2026-02-28T06:46:53Z","timestamp":1772261213990,"version":"3.50.1"},"update-to":[{"DOI":"10.1371\/journal.ppat.1011877","type":"new_version","label":"New version","source":"publisher","updated":{"date-parts":[[2024,1,5]],"date-time":"2024-01-05T00:00:00Z","timestamp":1704412800000}}],"reference-count":41,"publisher":"Public Library of Science (PLoS)","issue":"12","license":[{"start":{"date-parts":[[2023,12,21]],"date-time":"2023-12-21T00:00:00Z","timestamp":1703116800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100021778","name":"Agencia Nacional de Promoci\u00f3n de la Investigaci\u00f3n, el Desarrollo Tecnol\u00f3gico y la Innovaci\u00f3n","doi-asserted-by":"publisher","award":["PICT-2018-02338 and PICT2020-4010"],"award-info":[{"award-number":["PICT-2018-02338 and PICT2020-4010"]}],"id":[{"id":"10.13039\/501100021778","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100021778","name":"Agencia Nacional de Promoci\u00f3n de la Investigaci\u00f3n, el Desarrollo Tecnol\u00f3gico y la Innovaci\u00f3n","doi-asserted-by":"publisher","award":["PICT2020-2472"],"award-info":[{"award-number":["PICT2020-2472"]}],"id":[{"id":"10.13039\/501100021778","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100010269","name":"Wellcome Trust","doi-asserted-by":"publisher","award":["221859\/Z\/20\/Z"],"award-info":[{"award-number":["221859\/Z\/20\/Z"]}],"id":[{"id":"10.13039\/100010269","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100002923","name":"Consejo Nacional de Investigaciones Cient\u00edficas y T\u00e9cnicas","doi-asserted-by":"publisher","award":["PIBAA 28720210100978C"],"award-info":[{"award-number":["PIBAA 28720210100978C"]}],"id":[{"id":"10.13039\/501100002923","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["www.plospathogens.org"],"crossmark-restriction":false},"short-container-title":["PLoS Pathog"],"abstract":"<jats:p>Shiga-toxin producing <jats:italic>Escherichia coli<\/jats:italic> (STEC) infections can cause from bloody diarrhea to Hemolytic Uremic Syndrome. The STEC intestinal infection triggers an inflammatory response that can facilitate the development of a systemic disease. We report here that neutrophils might contribute to this inflammatory response by secreting Interleukin 1 beta (IL-1\u03b2). STEC stimulated neutrophils to release elevated levels of IL-1\u03b2 through a mechanism that involved the activation of caspase-1 driven by the NLRP3-inflammasome and neutrophil serine proteases (NSPs). Noteworthy, IL-1\u03b2 secretion was higher at lower multiplicities of infection. This secretory profile modulated by the bacteria:neutrophil ratio, was the consequence of a regulatory mechanism that reduced IL-1\u03b2 secretion the higher were the levels of activation of both caspase-1 and NSPs, and the production of NADPH oxidase-dependent reactive oxygen species. Finally, we also found that inhibition of NSPs significantly reduced STEC-triggered IL-1\u03b2 secretion without modulating the ability of neutrophils to kill the bacteria, suggesting NSPs might represent pharmacological targets to be evaluated to limit the STEC-induced intestinal inflammation.<\/jats:p>","DOI":"10.1371\/journal.ppat.1011877","type":"journal-article","created":{"date-parts":[[2023,12,21]],"date-time":"2023-12-21T18:59:05Z","timestamp":1703185145000},"page":"e1011877","update-policy":"https:\/\/doi.org\/10.1371\/journal.ppat.corrections_policy","source":"Crossref","is-referenced-by-count":5,"title":["Regulation of human neutrophil IL-1\u03b2 secretion induced by Escherichia coli O157:H7 responsible for hemolytic uremic syndrome"],"prefix":"10.1371","volume":"19","author":[{"given":"Florencia","family":"Sabbione","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Irene Angelica","family":"Keitelman","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Carolina Maiumi","family":"Shiromizu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alexia","family":"Vereertbrugghen","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Douglas","family":"Vera Aguilar","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Paolo Nahuel","family":"Rubatto Birri","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Manuela","family":"Pizzano","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mar\u00eda Victoria","family":"Ramos","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Federico","family":"Fuentes","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lucas","family":"Saposnik","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Agostina","family":"Cernutto","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Juliana","family":"Cassataro","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Carolina Cristina","family":"Jancic","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jerem\u00edas Gaston","family":"Galletti","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marina Sandra","family":"Palermo","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Anal\u00eda Silvina","family":"Trevani","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"340","published-online":{"date-parts":[[2023,12,21]]},"reference":[{"key":"ppat.1011877.ref001","doi-asserted-by":"crossref","first-page":"1722","DOI":"10.1016\/S0140-6736(22)01202-8","article-title":"Haemolytic uraemic syndrome","volume":"400","author":"Mini Michael","year":"2022","journal-title":"Lancet"},{"issue":"4","key":"ppat.1011877.ref002","doi-asserted-by":"crossref","first-page":"100","DOI":"10.3390\/microorganisms6040100","article-title":"Recent Advances in Shiga Toxin-Producing Escherichia coli Research in Latin America.","volume":"6","author":"A Torres","year":"2018","journal-title":"Microorganisms."},{"key":"ppat.1011877.ref003","doi-asserted-by":"crossref","first-page":"1019","DOI":"10.1189\/jlb.0308157","article-title":"Interactions between Shiga toxins and human polymorphonuclear leukocytes [Internet].","volume":"84","author":"M Brigotti","year":"2008","journal-title":"Journal of Leukocyte Biology"},{"key":"ppat.1011877.ref004","first-page":"1","article-title":"Shiga Toxin-Associated Hemolytic Uremic Syndrome: Care Management.","author":"B Travert","year":"2021"},{"issue":"10","key":"ppat.1011877.ref005","doi-asserted-by":"crossref","first-page":"827","DOI":"10.1002\/iub.1206","article-title":"Advances in pathogenesis and therapy of hemolytic uremic syndrome caused by Shiga toxin-2","volume":"65","author":"C Ibarra","year":"2013","journal-title":"IUBMB Life"},{"issue":"1","key":"ppat.1011877.ref006","doi-asserted-by":"crossref","first-page":"78","DOI":"10.1152\/ajpgi.90347.2008","article-title":"Macropinocytosis in Shiga toxin 1 uptake by human intestinal epithelial cells and transcellular transcytosis","volume":"21205","author":"I Malyukova","year":"2009","journal-title":"Am J Physiol\u2014Gastrointest Liver Physiol"},{"issue":"6","key":"ppat.1011877.ref007","doi-asserted-by":"crossref","first-page":"626","DOI":"10.3390\/toxins3060626","article-title":"Shiga toxin interaction with human intestinal epithelium.","volume":"3","author":"S. 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