{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T14:24:38Z","timestamp":1787063078024,"version":"build-2736575974"},"update-to":[{"DOI":"10.1371\/journal.ppat.1011903","type":"new_version","label":"New version","source":"publisher","updated":{"date-parts":[[2024,6,7]],"date-time":"2024-06-07T00:00:00Z","timestamp":1717718400000}}],"reference-count":94,"publisher":"Public Library of Science (PLoS)","issue":"5","license":[{"start":{"date-parts":[[2024,5,28]],"date-time":"2024-05-28T00:00:00Z","timestamp":1716854400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100020968","name":"Boehringer Ingelheim Animal Health","doi-asserted-by":"crossref","award":["BB\/T002727\/1"],"award-info":[{"award-number":["BB\/T002727\/1"]}],"id":[{"id":"10.13039\/100020968","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100000268","name":"Biotechnology and Biological Sciences Research Council","doi-asserted-by":"publisher","award":["BB\/T002727\/1"],"award-info":[{"award-number":["BB\/T002727\/1"]}],"id":[{"id":"10.13039\/501100000268","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100000849","name":"National Centre for the Replacement, Refinement and Reduction of Animals in Research","doi-asserted-by":"publisher","award":["NC\/N001486\/1"],"award-info":[{"award-number":["NC\/N001486\/1"]}],"id":[{"id":"10.13039\/501100000849","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100016337","name":"Department for the Economy, Northern Ireland","doi-asserted-by":"crossref","id":[{"id":"10.13039\/100016337","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/100016337","name":"Department for the Economy, Northern Ireland","doi-asserted-by":"crossref","id":[{"id":"10.13039\/100016337","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/100016337","name":"Department for the Economy, Northern Ireland","doi-asserted-by":"crossref","id":[{"id":"10.13039\/100016337","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/100016337","name":"Department for the Economy, Northern Ireland","doi-asserted-by":"crossref","id":[{"id":"10.13039\/100016337","id-type":"DOI","asserted-by":"crossref"}]},{"name":"Department for Agriculture, Environment and Rural Affairs, Northern Ireland"}],"content-domain":{"domain":["www.plospathogens.org"],"crossmark-restriction":false},"short-container-title":["PLoS Pathog"],"abstract":"<jats:p>\n                    The common liver fluke (\n                    <jats:italic>Fasciola hepatica<\/jats:italic>\n                    ) causes the disease fasciolosis, which results in considerable losses within the global agri-food industry. There is a shortfall in the drugs that are effective against both the adult and juvenile life stages within the mammalian host, such that new drug targets are needed. Over the last decade the stem cells of parasitic flatworms have emerged as reservoirs of putative novel targets due to their role in development and homeostasis, including at host-parasite interfaces. Here, we investigate and characterise the proliferating cells that underpin development in\n                    <jats:italic>F<\/jats:italic>\n                    .\n                    <jats:italic>hepatica<\/jats:italic>\n                    . We provide evidence that these cells are capable of self-renewal, differentiation, and are sensitive to ionising radiation\u2014 all attributes of neoblasts in other flatworms. Changes in cell proliferation were also noted during the early stages of\n                    <jats:italic>in vitro<\/jats:italic>\n                    juvenile growth\/development (around four to seven days post excystment), which coincided with a marked reduction in the nuclear area of proliferating cells. Furthermore, we generated transcriptomes from worms following irradiation-based ablation of neoblasts, identifying 124 significantly downregulated transcripts, including known stem cell markers such as\n                    <jats:italic>fgfrA<\/jats:italic>\n                    and\n                    <jats:italic>plk1<\/jats:italic>\n                    . Sixty-eight of these had homologues associated with neoblast-like cells in\n                    <jats:italic>Schistosoma mansoni<\/jats:italic>\n                    . Finally, RNA interference mediated knockdown of histone\n                    <jats:italic>h2b<\/jats:italic>\n                    (a marker of proliferating cells), ablated neoblast-like cells and impaired worm development\n                    <jats:italic>in vitro<\/jats:italic>\n                    . In summary, this work demonstrates that the proliferating cells of\n                    <jats:italic>F<\/jats:italic>\n                    .\n                    <jats:italic>hepatica<\/jats:italic>\n                    are equivalent to neoblasts of other flatworm species and demonstrate that they may serve as attractive targets for novel anthelmintics.\n                  <\/jats:p>","DOI":"10.1371\/journal.ppat.1011903","type":"journal-article","created":{"date-parts":[[2024,5,28]],"date-time":"2024-05-28T14:39:27Z","timestamp":1716907167000},"page":"e1011903","update-policy":"https:\/\/doi.org\/10.1371\/journal.ppat.corrections_policy","source":"Crossref","is-referenced-by-count":8,"title":["Neoblast-like stem cells of Fasciola hepatica"],"prefix":"10.1371","volume":"20","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-0030-1594","authenticated-orcid":true,"given":"Paul","family":"McCusker","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nathan G.","family":"Clarke","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Erica","family":"Gardiner","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rebecca","family":"Armstrong","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Erin M.","family":"McCammick","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Paul","family":"McVeigh","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Emily","family":"Robb","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Duncan","family":"Wells","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Madelyn","family":"Nowak-Roddy","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Abdullah","family":"Albaqami","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Angela","family":"Mousley","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jonathan A.","family":"Coulter","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"John","family":"Harrington","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nikki J.","family":"Marks","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Aaron G.","family":"Maule","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"340","published-online":{"date-parts":[[2024,5,28]]},"reference":[{"key":"ppat.1011903.ref001","volume-title":"Diseases in domestic animals caused by flukes. 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